The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
Role of mast cells in chronic stress induced colonic epithelial barrier dysfunction in the rat
Open accessAbstract
BACKGROUND AND AIMS: Stress may be an important factor in exacerbating inflammatory bowel disease but the underlying mechanism is unclear. Defective epithelial barrier function may allow uptake of luminal antigens that stimulate an immune/inflammatory response. Here, we examined the effect of chronic stress on colonic permeability and the participation of mast cells in this response. METHODS: Mast cell deficient Ws/Ws rats and +/+ littermate controls were submitted to water avoidance stress or sham stress (one hour/day) for five days. Colonic epithelial permeability to a model macromolecular antigen, horseradish peroxidase, was measured in Ussing chambers. Epithelial and mast cell morphology was studied by light and electron microscopy. RESULTS: Chronic stress significantly increased macromolecular flux and caused epithelial mitochondrial swelling in +/+ rats, but not in Ws/Ws rats, compared with non-stressed controls. Stress increased the number of mucosal mast cells and the proportion of cells showing signs of activation in +/+ rats. No mast cells or ultrastructural abnormalities of the epithelium were present in Ws/Ws rats. Increased permeability in +/+ rats persisted for 72 hours after stress cessation. CONCLUSIONS: Chronic stress causes an epithelial barrier defect and epithelial mitochondrial damage, in parallel with mucosal mast cell hyperplasia and activation. The study provides further support for an important role for mast cells in stress induced colonic mucosal pathophysiology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Mechanistically, inflammatory intestinal leukocytes, including IL17+ T cells as well as mast cells, may contribute to the breakdown of the intestinal epithelial barrier and the accompanying downregulation of tight junction proteins CLDN5, occludin (OCLN) and tight junction protein 2 (ZO-2)106,107.”
From Central regulation of stress-evoked peripheral immune responses (2023) · cites this paper as [106]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Mucosal mast cell degranulation within the gut can affect host intestinal integrity (Santos et al., 2001) and has been related to visceral pain (Barbara et al., 2004).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Santos et al., 2001
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Green, D. R., & Reed, J. C. (1998) · Science · doi:10.1126/science.281.5381.1309 · Cited by 9,051
Willner, P. (1997) · Psychopharmacology · doi:10.1007/s002130050456 · Cited by 1,832
Taurog, J. D., Richardson, J. A., Croft, J. T., Simmons, W. A., Zhou, M., FERNÁNDEZ-SUEIRO, J. L., Balish, E., & Hammer, R. E. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.180.6.2359 · Cited by 1,156
Effect of faecal stream diversion on recurrence of Crohn's disease in the neoterminal ileum
Rutgeerts, P., Peeters, M., Hiele, M. I., Vantrappen, G. R., Pennincx, F., Aerts, R., Kerremans, R., & Goboes, K. (1991) · The Lancet · doi:10.1016/0140-6736(91)90663-a · Cited by 758
II. Stress and intestinal barrier function
Söderholm, J. D., & Perdue, M. H. (2001) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.2001.280.1.g7 · Cited by 467
Self-citationStress and Exacerbation in Ulcerative Colitis: A Prospective Study of Patients Enrolled in Remission
Levenstein, S., Prantera, C., Varvo, V., Scribano, M. L., Andreoli, A., Luzi, C., Arcà, M., Berto, E., Milite, G., & Marcheggiano, A. (2000) · American Journal of Gastroenterology · doi:10.1111/j.1572-0241.2000.02012.x · Cited by 451
Gastrointestinal Illness and the Biopsychosocial Model
Drossman, D. A. (1998) · Psychosomatic Medicine · doi:10.1097/00006842-199805000-00007 · Cited by 388
Intestinal absorptive cell tight junctions are linked to cytoskeleton
Madara, J. (1987) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1987.253.1.c171 · Cited by 342
Somasundaram, S. G., Rafi, S., Hayllar, J. S., Sigthorsson, G., Jacob, M., Price, A. B., Macpherson, A. J., Mahmod, T., Scott, D. L., Wrigglesworth, J. M., et al. (1997) · Gut · doi:10.1136/gut.41.3.344 · Cited by 314
Aloe, L., Bracci‐Laudiero, L., Alleva, E., Lambìase, A., Micera, A., & Tirassa, P. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.22.10440 · Cited by 258
Qiu, B., Vallance, B. A., Blennerhassett, P. A., & Collins, S. M. (1999) · Nature Medicine · doi:10.1038/13503 · Cited by 252
Corticotropin-releasing hormone mimics stress-induced colonic epithelial pathophysiology in the rat
Santos, J., Saunders, P. R., Hanssen, N. P. M., Yang, P., Yates, D. A., Groot, J. A., & Perdue, M. H. (1999) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1999.277.2.g391 · Cited by 251
Self-citationDifferential release of serotonin and histamine from mast cells
Theoharides, T. C., Bondy, P. K., Tsakalos, N. D., & Askenase, P. W. (1982) · Nature · doi:10.1038/297229a0 · Cited by 244
Release of mast cell mediators into the jejunum by cold pain stress in humans
Santos, J., Saperas, E., Nogueiras, C., Mourelle, M., Antolín, M., Cadahia, A., & Malagelada, J. (1998) · Gastroenterology · doi:10.1016/s0016-5085(98)70577-3 · Cited by 237
Self-citationStress stimulates transepithelial macromolecular uptake in rat jejunum
Kiliaan, A. J., Saunders, P. R., Bijlsma, P. B., Berin, M. C., Taminiau, J. A., Groot, J. A., & Perdue, M. H. (1998) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1998.275.5.g1037 · Cited by 223
Self-citationTheoharides, T. C., Spanos, C., Pang, X., Alferes, L., Ligris, K., Letourneau, R., Rozniecki, J. J., Webster, E., & Chrousos, G. P. (1995) · Endocrinology · doi:10.1210/endo.136.12.7588332 · Cited by 214
Chronic stress impairs rat growth and jejunal epithelial barrier function: role of mast cells
Santos, J., Benjamin, M. A., Yang, P., Prior, T., & Perdue, M. H. (2000) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.2000.278.6.g847 · Cited by 205
Self-citationEpithelial permeability to proteins in the noninflamed ileum of Crohn's disease?
Söderholm, J. D., Peterson, K. H., Olaison, G., Franzén, L., Weström, B. R., Magnusson, K., & Sjödahl, R. I. (1999) · Gastroenterology · doi:10.1016/s0016-5085(99)70551-2 · Cited by 201
Self-citationTsukamoto, T., & Nigám, S. K. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.26.16133 · Cited by 191
Corticotropin‐Releasing Factor and the Brain‐Gut Motor Response to Stress
Taché, Y., Martı́nez, V., Million, M., & Rivier, J. (1999) · Canadian Journal of Gastroenterology and Hepatology · doi:10.1155/1999/375916 · Cited by 177
Perdue, M. H., Masson, S., Wershil, B. K., & Galli, S. J. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115047 · Cited by 162
Self-citationPrevious inflammation alters the response of the rat colon to stress
Collins, S. M., McHugh, K., Jacobson, K., Khan, I., Riddell, R. H., Murase, K., & Weingarten, H. (1996) · Gastroenterology · doi:10.1016/s0016-5085(96)70012-4 · Cited by 148
Anemia and mast cell depletion in mutant rats that are homozygous at "white spotting (Ws)" locus
Niwa, Y., Kasugai, T., Ohno, K., Morimoto, M., Yamazaki, M., Dohmae, K., Nishimune, Y., Kondo, K., & Kitamura, Y. (1991) · Blood · doi:10.1182/blood.v78.8.1936.1936 · Cited by 140
Wershil, B. K., Castagliuolo, I., & Pothoulakis, C. (1998) · Gastroenterology · doi:10.1016/s0016-5085(98)70315-4 · Cited by 135
Vagal Afferent Nerve Fibres Contact Mast Cells in Rat Small Intestinal Mucosa
Williams, R. M., Berthoud, H., & Stead, R. H. (2010) · NeuroImmunoModulation · doi:10.1159/000097346 · Cited by 128
Million, M., Taché, Y., & Anton, P. (1999) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1999.276.4.g1027 · Cited by 128
The Influence of Mast Cells on Pathways of Transepithelial Antigen Transport in Rat Intestine
Berin, M. C., Kiliaan, A. J., Yang, P., Groot, J. A., Kitamura, Y., & Perdue, M. H. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.5.2561 · Cited by 109
Self-citationDvořàk, A. M., McLeod, R. S., Onderdonk, A. B., Monahan-Earley, R. A., Cullen, J., Antonioli, D. A., Morgan, E. S., Blair, J. E., Estrella, P., Cisneros, R. L., et al. (2009) · International Archives of Allergy and Immunology · doi:10.1159/000236338 · Cited by 108
Cold-restraint stress increases rat fecal pellet output and colonic transit
Barone, F. C., Deegan, J. F., Price, W. J., Fowler, P. J., Fondacaro, J. D., & Ormsbee, H. S. (1990) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1990.258.3.g329 · Cited by 106
Colonic mucin release in response to immobilization stress is mast cell dependent
Castagliuolo, I., Wershil, B. K., Karalis, K. P., Pasha, A., Nikulásson, S. Þ., & Pothoulakis, C. (1998) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1998.274.6.g1094 · Cited by 102
Dvořàk, A. M., Tepper, R., Weller, P. F., Morgan, E., Estrella, P., Monahan-Earley, R., & Galli, S. J. (1994) · Blood · doi:10.1182/blood.v83.12.3600.3600 · Cited by 98
Review article: Intestinal permeability in Crohn's disease
Meddings, J. B. (1997) · Alimentary Pharmacology & Therapeutics · doi:10.1111/j.1365-2036.1997.tb00808.x · Cited by 98
Crowe, S. E., Luthra, G. K., & Perdue, M. H. (1997) · Gut · doi:10.1136/gut.41.6.785 · Cited by 97
Self-citationStress and intestinal barrier function
Wang, H. (2002) · Parenteral & Enteral Nutrition · Cited by 97
Degranulation of Basophilic Leukocytes in Allergic Contact Dermatitis Reactions in Man
Dvořàk, A. M., Mihm, M. C., & Dvorak, H. F. (1976) · The Journal of Immunology · doi:10.4049/jimmunol.116.3.687 · Cited by 92
Intestinal permeability in Crohn's disease.
Murphy, M., EASTHAM, E. J., NELSON, R. L., Pearson, A., & Laker, M. F. (1989) · Archives of Disease in Childhood · doi:10.1136/adc.64.3.321 · Cited by 76
Horiguchi, K., & Komuro, T. (1998) · Cell and Tissue Research · doi:10.1007/s004410051119 · Cited by 64
Coelho, A.-M., Fioramonti, J., & Buéno, L. (2000) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.2000.279.4.g781 · Cited by 63
Cirulli, F., Pistillo, L., Acetis, L. D., Alleva, E., & Aloe, L. (1998) · Brain Behavior and Immunity · doi:10.1006/brbi.1998.0505 · Cited by 61
Neuro-Immune Pathobiology of Infectious Enteric Disease
Argenzio, R. A. (1997) · Advances in experimental medicine and biology · doi:10.1007/978-1-4899-1828-4_2 · Cited by 16
Repeated antigen challenge in rats induces a mucosal mast cell hyperplasia
Marshall, J. S. (1993) · Gastroenterology · doi:10.1016/0016-5085(93)90712-l · Cited by 11
The response of rat colonic mucosa to 5-hydroxytryptamine in health and following restraint stress
Goldhill, J., Porquet, M.-F., & Angel, I. (1998) · European Journal of Pharmacology · doi:10.1016/s0014-2999(98)00420-8 · Cited by 10
Hori, T., Wanibuchi, H., Yano, Y., Otani, S., Nishikawa, A., Osugi, H., Kinoshita, H., & Fukushima, S. (1998) · Cancer Letters · doi:10.1016/s0304-3835(97)00504-1 · Cited by 9
Influence of maturation on activity-stress related pathology in the rat colon
Andrianopoulos, G. D., Bombeck, C. T., & Nyhus, L. M. (1986) · Physiology & Behavior · doi:10.1016/0031-9384(86)90487-7 · Cited by 6
Ultrastructure of the duodenal mucosa of rats under stress
Perov, Y. L., & Evdokimov, V. V. (1976) · Bulletin of Experimental Biology and Medicine · doi:10.1007/bf00797164 · Cited by 4
Research works printed but not linked: 1
- 10.1210/en.136.12.5745 · DOI · Paper page
Cited by
Sorted by citations
Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286
Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour
Cryan, J. F., & Dinan, T. G. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3346 · Cited by 4,572
Martin, C. R., Osadchiy, V., Kalani, A., & Mayer, E. A. (2018) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2018.04.003 · Cited by 1,284
Psychobiotics and the manipulation of bacteria–gut–brain signals
Sarkar, A., Lehto, S. M., Harty, S., Dinan, T. G., Cryan, J. F., & Burnet, P. W. J. (2016) · Trends in Neurosciences · doi:10.1016/j.tins.2016.09.002 · Cited by 1,068
The role of microbiome in central nervous system disorders
Wang, Y., & Kasper, L. H. (2014) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2013.12.015 · Cited by 803
Metadata
- Authors
- Santos, J., Yang, P.-C., Söderholm, J. D., Benjamin, M., & Perdue, M. H.
- Affiliations
- McMaster University (CA)
- Corresponding author
- Perdue, M H
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2001-05-01
- Volume
- 48
- Issue
- 5
- Pages
- 630-636
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1728291
- OpenAlex
- W2141354880
- Cited by
- 313
- References
- 46
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 5 · 2023: 10 · 2022: 11 · 2021: 11 · 2020: 16 · 2019: 18 · 2018: 13 · 2017: 12 · 2016: 13 · 2015: 13 · 2014: 10 · 2013: 16 · 2012: 22
- FWCI
- 6.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
- Keywords
- mast cells, chronic stress, colonic permeability, inflammatory bowel disease, water avoidance stress, mitochondrial swelling, mast cell activation
- MeSH terms
- Analysis of Variance, Animals, Cell Membrane Permeability, Electric Conductivity, Gastrointestinal Transit, Horseradish Peroxidase, Intestinal Mucosa, Mast Cells, Microscopy, Electron, Mitochondria, Rats, Mutant Strains, Stress, Psychological, Statistics, Nonparametric, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
