Fatty acids and epithelial permeability: effect of conjugated linoleic acid in Caco-2 cells
Open accessAbstract
Conjugated linoleic acid (CLA) is a collective term referring to the positional and geometric isomers of linoleic acid. This novel fatty acid has been shown to have a number of beneficial actions, including immunomodulatory, anticarcinogenic, and antiatherogenic effects. Tight junctions of epithelial cells determine epithelial membrane integrity and selective paracellular permeability to ions and macromolecules. Occludin and ZO-1 are integral structural components of the tight junction, which are involved in the biogenesis and functional integrity of the epithelial monolayer. This study investigated the effects of two isomers of CLA (cis-9 and trans-10 isomers) on Caco-2 cell transepithelial resistance (TER) development, paracellular epithelial permeability, and occludin and ZO-1 expression. Caco-2 cells were grown in media supplemented with 0.05 mM linoleic acid, cis-9 CLA, or trans-10 CLA for 21 days. The trans-10 CLA isomer delayed Caco-2 cell TER development, which is an in vitro measure of epithelial cell integrity, and increased paracellular epithelial permeability. Immunofluorescent staining of Caco-2 cell epithelial monolayers grown in media supplemented trans-10 CLA showed that the trans-10 CLA isomer altered distribution of occludin and ZO-1. The trans-10 CLA isomer delayed the acquisition of transepithelial resistance and altered the cellular distribution of occludin, which have important implications in relation to epithelial permeability.
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Treatment with the trans-10 CLA isomer caused a redistribution of ZO-1 and OCLN and increased paracellular permeability in Caco-2 colon epithelial cells.
“Treatment with the trans-10 CLA isomer caused a redistribution of ZO-1 and OCLN and increased paracellular permeability in Caco-2 colon epithelial cells.119”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [119]
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Regulation of Tight Junction Permeability by Intestinal Bacteria and Dietary Components1,2
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Regulation of Intestinal Barrier Function by Microbial Metabolites
Ghosh, S., Whitley, C. S., Haribabu, B., & Jala, V. R. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.02.007 · Cited by 835 · see citing works
- Authors
- Roche, H. M., Terres, A. M., Black, I. B., Gibney, M. J., & Kelleher, D.
- Affiliations
- Trinity College Dublin (IE)
- Corresponding author
- Roche, H M
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2001-06-01
- Volume
- 48
- Issue
- 6
- Pages
- 797-802
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1728326
- OpenAlex
- W2138692667
- Cited by
- 67
- References
- 25
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 4 · 2022: 1 · 2021: 3 · 2020: 3 · 2018: 3 · 2017: 3 · 2016: 5 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 5
- FWCI
- 3.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Arrow@dit (Dublin Institute of Technology) (submittedVersion, cc-by-nc-sa)
- HighWire Press Open Archive (submittedVersion)
- Trinity's Access to Research Output (TARA) (Trinity College Dublin) (submittedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- conjugated linoleic acid, tight junctions, occludin, ZO-1, epithelial permeability, transepithelial electrical resistance, Caco-2 cells, intestinal epithelium, linoleic acid, paracellular permeability
- MeSH terms
- Analysis of Variance, Animals, Cell Membrane Permeability, Diuretics, Osmotic, Epithelial Attachment, Humans, Linoleic Acids, Mannitol, Caco-2 Cells
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