Mucin gene expression in intestinal epithelial cells in Crohn's disease
Open accessAbstract
BACKGROUND: Crohn's disease (CD) is a chronic relapsing inflammatory bowel disease of unknown origin. It is characterised by chronic mucosal ulcerations which affect any part of the intestine but most commonly are found in the ileum and proximal colon. AIMS: Studies were undertaken to provide information regarding cell specific expression of mucin genes in the ileum of patients with CD. PATIENTS AND METHODS: Expression of mucin genes was analysed in the ileal mucosa of patients with CD and controls by in situ hybridisation and immunohistochemistry. RESULTS: In healthy ileal mucosa, patients with CD showed a pattern identical to normal controls with main expression of MUC2 and MUC3 , lesser expression of MUC1 and MUC4 , and no expression of MUC5AC , MUC5B , MUC6 , or MUC7 . In the involved mucosa, the pattern was somewhat comparable although heterogeneous to that observed in healthy ileal mucosa. Importantly, a particular mucin gene expression pattern was observed in ileal mucosa close to the ulcer margins in ulcer associated cell lineage, with the appearance of MUC5AC and MUC6 mRNAs and peptides, which are normally restricted to the stomach ( MUC5AC and MUC6 ) and duodenum ( MUC6 ), and disappearance of MUC2 . CONCLUSIONS: Our results suggest that gel forming mucins (more particularly MUC5AC and MUC6) may have a role in epithelial wound healing after mucosal injury in inflammatory bowel diseases in addition to mucosal protection.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
MUC2 gene levels were found to be altered in both ulcerative colitis and Crohn's disease compared to healthy controls.
“MUC2 gene levels were found to be altered in both UC and CD compared to healthy controls.119,120”
From The emerging roles of bacterial proteases in intestinal diseases (2023) · cites this paper as [120]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Riddell, R. H., Goldman, H., Ransohoff, D. F., Appelman, H. D., Fenoglio, C. M., Haggitt, R. C., hren, C., Correa, P., Hamilton, S. R., Morson, B. C., et al. (1983) · Human Pathology · doi:10.1016/s0046-8177(83)80175-0 · Cited by 1,857 · see citing works
Gendler, S. J., & Spicer, A. P. (1995) · Annual Review of Physiology · doi:10.1146/annurev.ph.57.030195.003135 · Cited by 1,001 · see citing works
The Structure and Assembly of Secreted Mucins
Pérez-Vilar, J., & Hill, R. L. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.45.31751 · Cited by 527 · see citing works
Wright, N. A., Pike, C. M., & Elia, G. (1990) · Nature · doi:10.1038/343082a0 · Cited by 466 · see citing works
Audie, J. P., Janin, A., Porchet, N., Copin, M., Gosselin, B. H., & Aubert, J. (1993) · Journal of Histochemistry & Cytochemistry · doi:10.1177/41.10.8245407 · Cited by 458 · see citing works
Self-citationDignass, A. U., Lynch–Devaney, K., Kindon, H., Thim, L., & Podolsky, D. K. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117332 · Cited by 422 · see citing works
CURRENT CONCEPTS OF THE ETIOLOGY AND PATHOGENESIS OF ULCERATIVE COLITIS AND CROHN’S DISEASE
Sartor, R. B. (1995) · Gastroenterology Clinics of North America · doi:10.1016/s0889-8553(21)00207-7 · Cited by 384 · see citing works
Kindon, H., Pothoulakis, C., Thim, L., Lynch–Devaney, K., & Podolsky, D. K. (1995) · Gastroenterology · doi:10.1016/0016-5085(95)90340-2 · Cited by 337 · see citing works
Mucin gene structure and expression: protection vs. adhesion
van Klinken, B. J., Dekker, J. P., Büller, H. A., & Einerhand, A. W. C. (1995) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1995.269.5.g613 · Cited by 319 · see citing works
Wickström, C., Davies, J. R., Eriksen, G. V., Veerman, E. C. I., & Carlstedt, I. (1998) · Biochemical Journal · doi:10.1042/bj3340685 · Cited by 315 · see citing works
Two novel mucin genes down-regulated in colorectal cancer identified by differential display.
Williams, S. J., McGuckin, M. A., Gotley, D. C., Eyre, H. J., Sutherland, G. R., & Antalis, T. (1999) · PubMed · Cited by 300 · see citing works
Mucin gene expression in normal, preneoplastic, and neoplastic human gastric epithelium.
Ho, S. B., Shekels, L. L., Toribara, N. W., et al. (1995) · PubMed · Cited by 284 · see citing works
Podolsky, D. K., Lynch–Devaney, K., Stow, J. L., Oates, P. S., Murgue, B., deBeaumont, M., Sands, B. E., & Mahida, Y. R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53305-6 · Cited by 267 · see citing works
Trefoil peptide gene expression in gastrointestinal epithelial cells in inflammatory bowel disease
Wright, N. A., Poulsom, R., Stamp, G. W. H., Noorden, S. V., Sarraf, C., Elia, G., Ahnen, D. J., Jeffery, R. E., Longcroft, J. M., Pike, C. M., et al. (1993) · Gastroenterology · doi:10.1016/0016-5085(93)90830-6 · Cited by 264 · see citing works
Healing of severe recurrent ileitis with azathioprine therapy in patients with Crohn's disease
D’Haens, G., Geboes, K., Ponette, E., et al. (1997) · Gastroenterology · doi:10.1016/s0016-5085(97)70027-1 · Cited by 250 · see citing works
Incidence of inflammatory bowel disease in northern France (1988-1990).
Gower‐Rousseau, C., Salomez, J., Dupas, J. L., Marti, R., Nuttens, M. C., Votte, A., Lemahieu, M., Lemaire, B., Colombel, J., & Cortot, A. (1994) · Gut · doi:10.1136/gut.35.10.1433 · Cited by 241 · see citing works
Self-citationMUC5AC, but not MUC2, is a prominent mucin in respiratory secretions
HOVENBERG, H. W., Davies, J. R., Herrmann, A., Lind�n, C.-J., & Carlstedt, I. (1996) · Glycoconjugate Journal · doi:10.1007/bf00702348 · Cited by 230 · see citing works
Human Mucin Genes Assigned to 11p15.5: Identification and Organization of a Cluster of Genes
Pigny, P., Guyonnet‐Dupérat, V., Hill, A. S., Pratt, W. S., Galiègue‐Zouitina, S., Collyn-d'Hooghe, M., Laine, A., SEUNINGEN, I. V., Degand, P., Gum, J. R., et al. (1996) · Genomics · doi:10.1006/geno.1996.0637 · Cited by 223 · see citing works
Self-citationTomasetto, C., Rio, M., Gautier, C., Wolf, C., Hareuveni, M., Chambon, P., & Lathe, R. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08125.x · Cited by 216 · see citing works
Trefoil peptides: from structure to function
Thim, L. (1997) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050108 · Cited by 216 · see citing works
Sands, B. E., & Podolsky, D. K. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.001345 · Cited by 216 · see citing works
Developmental Mucin Gene Expression in the Human Respiratory Tract
Buisine, M., Devisme, L., Copin, M., et al. (1999) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.20.2.3259 · Cited by 194 · see citing works
Self-citationLongman, R. J., Douthwaite, J., Sylvester, P. A., et al. (2000) · Gut · doi:10.1136/gut.47.6.792 · Cited by 190 · see citing works
Hanby, A. M., Poulsom, R., Singh, S., et al. (1993) · Gastroenterology · doi:10.1016/0016-5085(93)90956-d · Cited by 183 · see citing works
Induction of pS2 and hSP genes as markers of mucosal ulceration of the digestive tract
Rio, M., Chenard, M., Wolf, C., Marcellin, L., Tomasetto, C., Lathe, R., Bellocq, J., & Chambon, P. (1991) · Gastroenterology · doi:10.1016/0016-5085(91)90205-y · Cited by 178 · see citing works
pS2/TFF1 interacts directly with the VWFC cysteine-rich domains of mucins
Tomasetto, C., Masson, R., Linares, J., Wendling, C., Lefèbvre, O., Chenard, M., & Rio, M. (2000) · Gastroenterology · doi:10.1016/s0016-5085(00)70415-x · Cited by 177 · see citing works
Mucin gene expression in human embryonic and fetal intestine
Buisine, M., Devisme, L., Savidge, T., et al. (1998) · Gut · doi:10.1136/gut.43.4.519 · Cited by 173 · see citing works
Self-citationAllelic polymorphism and chromosomal localization of the human oviductin gene (MUC9)
Lapensée, L., Paquette, Y., & Bleau, G. (1997) · Fertility and Sterility · doi:10.1016/s0015-0282(97)00317-8 · Cited by 169 · see citing works
Hauser, F., Poulsom, R., Chinery, R., et al. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.15.6961 · Cited by 165 · see citing works
Reis, C. A., David, L., Carvalho, F., et al. (2000) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540004800307 · Cited by 163 · see citing works
A novel human airway mucin cDNA encodes a protein with unique tandem-repeat organization
Shankar, V., Gilmore, M. S., Elkins, R. C., & Sachdev, G. P. (1994) · Biochemical Journal · doi:10.1042/bj3000295 · Cited by 128 · see citing works
Expression of MUC2 and MUC3 mRNA in human normal, malignant, and inflammatory intestinal tissues.
Weiss, A. A., Babyatsky, M. W., Ogata, S., et al. (1996) · Journal of Histochemistry & Cytochemistry · doi:10.1177/44.10.8813081 · Cited by 128 · see citing works
E-cadherin/catenin complex in benign and malignant melanocytic lesions
Silye, R., Karayiannakis, A. J., Syrigos, K. N., Poole, S., Noorden, S. V., Batchelor, W., Regele, H. M., Sega, W., Boesmueller, H., Krausz, T. N., et al. (1998) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(199812)186:4 · Cited by 120 · see citing works
Abnormalities in Mucin Gene Expression in Crohnʼs Disease
Buisine, M., Desreumaux, P., Debailleul, V., Gambiez, L. P., Geboes, K., Ectors, N., Delescaut, M.-P., Degand, P., Aubert, J., Colombel, J., et al. (1999) · Inflammatory Bowel Diseases · doi:10.1097/00054725-199902000-00004 · Cited by 115 · see citing works
Self-citationBuisine, M., Devisme, L., Degand, P., et al. (2000) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540004801210 · Cited by 115 · see citing works
Self-citationEDITORIAL. p53—INTEGRATING THE COMPLEXITY
Hall, P. A., Meek, D. W., & Lane, D. P. (1996) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(199609)180:1 · Cited by 112 · see citing works
The Carboxyl-terminal Sequence of the Human Secretory Mucin, MUC6
Toribara, N. W., Ho, S. B., Gum, E. T., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.26.16398 · Cited by 111 · see citing works
Buisine, M., Devisme, L., Maunoury, V., et al. (2000) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540004801209 · Cited by 109 · see citing works
Self-citationTrefoil Peptide Gene Expression in Gastrointestinal Epithelial Cells in Inflammatory Bowel Disease
Wright, N. A., Poulsom, R., Stamp, G., Norden, S., Sarraf, C., Elia, G., Ahnen, D. J., Jeffery, R. E., Longcroft, J. M., Pike, C. M., et al. (1992) · Scandinavian Journal of Gastroenterology · doi:10.3109/00365529209096010 · Cited by 100 · see citing works
Mucins in normal and neoplastic human gastrointestinal tissues
Lesuffleur, T., Zweibaum, A., & Real, F. X. (1994) · Critical Reviews in Oncology/Hematology · doi:10.1016/1040-8428(94)90053-1 · Cited by 98 · see citing works
Gendler, S. (1995) · Annual Review of Physiology · doi:10.1146/annurev.physiol.57.1.607 · Cited by 84 · see citing works
Ahnen, D. J., Poulsom, R., Stamp, G. W. H., Elia, G., Pike, C. M., Jeffery, R. E., Longcroft, J. M., Rio, M., Chambon, P., & Wright, N. A. (1994) · The Journal of Pathology · doi:10.1002/path.1711730406 · Cited by 58 · see citing works
Human spasmolytic polypeptide decreases proton permeation through gastric mucus in vivo and in vitro
Tanaka, S., Podolsky, D. K., Engel, E., et al. (1997) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1997.272.6.g1473 · Cited by 58 · see citing works
Podolsky, D. K., Lynch–Devaney, K., Stow, J. L., et al. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50330-1 · Cited by 53 · see citing works
Seib, T., Blin, N., Hilgert, K., et al. (1997) · Genomics · doi:10.1006/geno.1996.4511 · Cited by 49 · see citing works
Klein, O., Colombel, J.-F., Lescut, D., et al. (1995) · PubMed · Cited by 45 · see citing works
Self-citationAbnormalities in mucin gene expression in Crohn's disease
Buisine, M., Desreumaux, P., Debailleul, V., Gambiez, L. P., Geboes, K., Ectors, N., Delescaut, M.-P., Degand, P., Aubert, J., Colombel, J., et al. (2007) · Inflammatory Bowel Diseases · doi:10.1002/ibd.3780050105 · Cited by 27 · see citing works
Self-citationRoberts, I. S. D., & Stoddart, R. W. (1993) · The Journal of Pathology · doi:10.1002/path.1711710105 · Cited by 26 · see citing works
Sands, B. E. (1996) · Annual Review of Physiology · doi:10.1146/annurev.physiol.58.1.253 · Cited by 18 · see citing works
Geboes, K. J., Desreumaux, P., Jouret, A., et al. (1999) · PubMed · Cited by 14 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 2
- Corfield AP, Warren BF. Mucus glycoproteins and their role in colorectal disease. J Pathol 1996;180:8-17.
- Bartman AE, Buisine MP, Aubert JP, et al. The MUC6 secretory mucin gene is expressed in a wide variety of epi- thelial tissues. J Pathol 1998;186:398-405.
Cited by
Sorted by citations
Inflammatory bowel disease: cause and immunobiology
Baumgart, D. C., & Carding, S. R. (2007) · The Lancet · doi:10.1016/s0140-6736(07)60750-8 · Cited by 2,147 · see citing works
Van der Sluis, M., De Koning, B. A. E., De Bruijn, A. C. J. M., Velcich, A., Meijerink, J. P. P., Van Goudoever, J. B., Büller, H. A., Dekker, J., Van Seuningen, I., Renes, I. B., et al. (2006) · Gastroenterology · doi:10.1053/j.gastro.2006.04.020 · Cited by 1,684 · see citing works
Barrier Epithelial Cells and the Control of Type 2 Immunity
Hammad, H., & Lambrecht, B. N. (2015) · Immunity · doi:10.1016/j.immuni.2015.07.007 · Cited by 847 · see citing works
Gaudier, E., Jarry, A., Blottière, H. M., de Coppet, P., Buisine, M. P., Aubert, J. P., Laboisse, C., Cherbut, C., & Hoebler, C. (2004) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00219.2004 · Cited by 365 · see citing works
Self-citationThe emerging roles of bacterial proteases in intestinal diseases
Caminero, A., Guzman, M., Libertucci, J., & Lomax, A. E. (2023) · Gut Microbes · doi:10.1080/19490976.2023.2181922 · Cited by 92 · see citing works
- Authors
- Buisine, M.-P., Desreumaux, P., Leteurtre, E., Copin, M.-C., Colombel, J.-F., Porchet, N., & Aubert, J.-P.
- Affiliations
- Inserm (FR)
- Corresponding author
- Buisine, M-P
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2001-10-01
- Volume
- 49
- Issue
- 4
- Pages
- 544-551
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1728475
- OpenAlex
- W2113474033
- Cited by
- 195
- References
- 50
- Citations by year
- 2026: 12 · 2025: 9 · 2024: 10 · 2023: 14 · 2022: 7 · 2021: 8 · 2020: 6 · 2019: 8 · 2018: 8 · 2017: 8 · 2016: 5 · 2015: 12 · 2014: 8 · 2013: 9 · 2012: 3
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Keywords
- Crohn's disease, mucin gene expression, intestinal epithelial cells, ileal mucosa, MUC2, MUC5AC, MUC6, gel-forming mucins, ulcer healing, mucosal protection, inflammatory bowel disease, MUC3, MUC1, MUC4
- MeSH terms
- Adolescent, Adult, Aged, Cell Line, Crohn Disease, Epithelial Cells, Female, Humans, Ileum, Intestinal Mucosa, Male, Middle Aged, Mucins, RNA, Messenger, Staining and Labeling, Gene Expression, Case-Control Studies, In Situ Hybridization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
