Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose
Abstract
The mucus layer covering the gastrointestinal mucosa is considered the first line of defense against aggressions arising from the luminal content. It is mainly composed of high molecular weight glycoproteins called mucins. Butyrate, a short-chain fatty acid produced during carbohydrate fermentation, has been shown to increase mucin secretion. The aim of this study was to test 1) whether butyrate regulates the expression of various MUC genes, which are coding for protein backbones of mucins, and 2) whether this effect depends on butyrate status as the major energy source of colonocytes. Butyrate was provided at the apical side of human polarized colonic goblet cell line HT29-Cl.16E in glucose-rich or glucose-deprived medium. In glucose-rich medium, butyrate significantly increased MUC3 and MUC5B expression (1.6-fold basal level for both genes), tended to decrease MUC5AC expression, and had no effect on MUC2 expression. In glucose-deprived medium, i.e., when butyrate was the only energy source available, MUC3 and MUC5B increase persisted, whereas MUC5AC expression was significantly enhanced (3.7-fold basal level) and MUC2 expression was strikingly increased (23-fold basal level). Together, our findings show that butyrate is able to upregulate colonic mucins at the transcriptional level and even better when it is the major energy source of the cells. Thus the metabolism of butyrate in colonocytes is closely linked to some of its gene-regulating effects. The distinct effects of butyrate according to the different MUC genes could influence the composition and properties of the mucus gel and thus its protective function.
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SCFAs support the integrity of the intestinal mucosa in vitro and in vivo.
“which have been shown in vitro and in vivo to support the integrity of the intestinal mucosa in a variety of ways (Fukuda et al., 2011; Gaudier et al., 2004; Macia et al., 2015; Willemsen et al., 2003; Wrzosek et al., 2013).”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Gaudier et al., 2004
Butyrate stimulates intestinal mucus production.
“stimulates intestinal mucus production [235]”
From Gut microbiota role in irritable bowel syndrome: New therapeutic strategies (2016) · cites this paper as [235]
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- Authors
- Gaudier, E., Jarry, A., Blottière, H. M., de Coppet, P., Buisine, M. P., Aubert, J. P., Laboisse, C., Cherbut, C., & Hoebler, C.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Nantes Université (FR)
- Département Alimentation Humaine (FR)
- Inserm (FR)
- PhysioPathologie des Adaptations Nutritionnelles (FR)
- Venue
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Publisher
- American Physiological Society
- Published
- 2004-12
- Volume
- 287
- Issue
- 6
- Pages
- G1168-G1174
- ISSN
- 0193-1857 (ISSN-L)
- 1522-1547
- OpenAlex
- W2136633470
- Cited by
- 365
- References
- 54
- Citations by year
- 2026: 22 · 2025: 34 · 2024: 30 · 2023: 37 · 2022: 32 · 2021: 37 · 2020: 21 · 2019: 19 · 2018: 12 · 2017: 21 · 2016: 15 · 2015: 7 · 2014: 7 · 2013: 12 · 2012: 13
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- World Cancer Research Fund International
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- butyrate, MUC genes, mucin, goblet cells, colonic epithelium, glucose deprivation, MUC3, MUC5B, MUC5AC, MUC2, gene expression regulation, short-chain fatty acids, mucus layer
- MeSH terms
- Butyrates, Culture Media, Dose-Response Relationship, Drug, Enzyme Inhibitors, Fatty Acids, Gene Expression Regulation, Glucose, Humans, Hydrogen-Ion Concentration, Hydroxamic Acids, Intestinal Mucosa, L-Lactate Dehydrogenase, Mucins, RNA, Messenger, Time Factors, Cell Death, HT29 Cells, Reverse Transcriptase Polymerase Chain Reaction, Goblet Cells, Mucin-2, Mucin-3
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