Minimal alteration in muscle lipid genes following stabilized weight loss
Open accessAbstract
Variations in skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), carntine palmitoyltransferase-1 (CPT-1), perilipin protein 2 (PLIN2), and adipose tissue triglyceride lipase (ATGL), and comparative gene identification-58 (CGI-58) have been described as playing important roles in the metabolic regulation of lipid oxidation, and may influence intramyocellular lipid (IMCL) and muscle lipid droplet size (LDS). While acute changes in caloric balance and/or aerobic capacity may affect lipid metabolism, the influence of sustained weight loss derived from caloric restriction with weight loss (CWL) compared with exercise training with weight loss (EWL) on the abovementioned parameters has not been fully elucidated. Using a combination of metabolic feeding and/or supervised exercise training, we evaluated the influence of stabilized weight loss elicited by CWL compared with EWL without the confounding influence of acute alterations in caloric balance on molecular markers of mitochondrial metabolism and lipid droplet size in middle-aged overweight individuals with impaired glucose tolerance. There were no significant changes in PGC-1α, CPT-1, PLIN2, ATGL and, CGI-58 messenger RNA (mRNA) in CWL and EWL. While there were no changes in ATGL mRNA in CWL, there was a strong trend (P = 0.05) for the ΔATGL mRNA in EWL with stabilized weight loss. There were no significant changes in IMCL or LDS within skeletal muscle in CWL or EWL, respectively. In conclusion, under the conditions of chronic caloric balance following dietary or exercise-based interventions, mediators of mitochondrial function, IMCL and LDS, were largely unaffected. Future studies should focus on intervention-based changes in protein expression and/or phosphorylation and the relationship to physiological endpoints.
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The effects of caloric restriction on intramyocellular lipids are mixed, with studies reporting increases, decreases and no change.
“the effects of CR have been shown to be mixed, with studies reporting increases, decreases and no change [124–127].”
From Targeting Intramyocellular Lipids to Improve Aging Muscle Function (2025) · cites this paper as [125]
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Targeting Intramyocellular Lipids to Improve Aging Muscle Function
Russ, D. W., Manickam, R., & Tipparaju, S. M. (2025) · Lipids in Health and Disease · doi:10.1186/s12944-025-02622-6 · Cited by 15 · see citing works
- Authors
- Coker, R. H., Robinette, L., & Kern, P. A.
- Affiliations
- University of Alaska Fairbanks (US)
- University of Arkansas for Medical Sciences (US)
- University of Kentucky (US)
- Venue
- Applied Physiology, Nutrition, and Metabolism
- Publisher
- Canadian Science Publishing
- Published
- 2017-12
- Volume
- 42
- Issue
- 12
- Pages
- 1277-1282
- ISSN
- 1715-5312 (ISSN-L)
- 1715-5320
- OpenAlex
- W2743269720
- Cited by
- 3
- References
- 32
- Citations by year
- 2025: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Applied Physiology Nutrition and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (acceptedVersion)
- Funders
- American Heart Association
- National Institutes of Health (K01 DK, UL1TR001998)
- National Institute on Aging (P30AG028718)
- National Institute of Diabetes and Digestive and Kidney Diseases (K01DK064716)
- National Center for Research Resources (M01RR014288)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- skeletal muscle lipid metabolism, intramyocellular lipid, lipid droplet size, PGC-1α, carnitine palmitoyltransferase 1, PLIN2, adipose triglyceride lipase, CGI-58, mitochondrial metabolism, impaired glucose tolerance, gene expression
- MeSH terms
- Aged, Aged, 80 and over, Female, Gene Expression Regulation, Humans, Male, Middle Aged, Muscle Proteins, Weight Loss, Exercise, Muscle, Skeletal, Caloric Restriction, Lipid Metabolism
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