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Cited by 292Cardiovascular and exercise physiologyPeroxisome Proliferator-Activated ReceptorsAdipose Tissue and Metabolism

Exercise training increases lipid metabolism gene expression in human skeletal muscle

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Tunstall, Rebecca J., Mehan, Kate A., Wadley, Glenn D., Collier, Gregory R., Bonen, Arend, Hargreaves, Mark, Cameron-Smith, David·2002·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00475.2001
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Abstract

The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and β-oxidation of fatty acids (FA), together with the gene expression of transcription factors implicated in the regulation of FA homeostasis were investigated. Seven human subjects (3 male, 4 female, 28.9 ± 3.1 yr of age, range 20–42 yr, body mass index 22.6 kg/m 2, range 17–26 kg/m 2) underwent a 9-day exercise training program of 60 min cycling per day at 63% peak oxygen uptake (V˙o 2 peak; 104 ± 14 W). On days 1 and 9 of the program, muscle biopsies were sampled from the vastus lateralis muscle at rest, at the completion of exercise, and again 3 h postexercise. Gene expression of key components of FA transport [FA translocase (FAT/CD36), plasma membrane-associated FA-binding protein], β-oxidation [carntine palmitoyltransferase(CPT) I, β-hydroxyacyl-CoA dehydrogenase] and transcriptional control [peroxisome proliferator-activated receptor (PPAR)α, PPARγ, PPARγ coactivator 1, sterol regulatory element-binding protein-1c] were unaltered by exercise when measured at the completion and at 3 h postexercise. Training increased total lipid oxidation by 24% ( P < 0.05) for the 1-h cycling bout. This increased capacity for lipid oxidation was accompanied by an increased expression of FAT/CD36 and CPT I mRNA. Similarly, FAT/CD36 protein abundance was also upregulated by exercise training. We conclude that enhanced fat oxidation after exercise training is most closely associated with the genes involved in regulating FA uptake across the plasma membrane (FAT/CD36) and across the mitochondrial membrane (CPT I).

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  • The effect of exercise on glucose tolerance and insulin sensitivity is probably due to increased CPT1 expression and activity during exercise.

    “This effect is probably due to increased CPT1 expression and activity during exercise ([163](#B163));”

    From Mitochondrial fatty acid oxidation in obesity (2013) · cites this paper as [163]

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Authors
Tunstall, R. J., Mehan, K. A., Wadley, G. D., Collier, G. R., Bonen, A., Hargreaves, M., & Cameron-Smith, D.
Affiliations
  • Deakin University (AU)
  • School of Health Sciences, Deakin University, Burwood, Victoria 3125;
  • University of Waterloo (CA)
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2002-07-01
Volume
283
Issue
1
Pages
E66-E72
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00475.2001
OpenAlex
W2094288633
Semantic Scholar
02cc9c5664d9ca524e044a3c9024b3f7ff6ee9f5
Cited by
292
References
42
Citations by year
2026: 4 · 2025: 2 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 9 · 2020: 16 · 2019: 8 · 2018: 7 · 2017: 9 · 2016: 17 · 2015: 10 · 2014: 12 · 2013: 15 · 2012: 15
FWCI
42.55
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Deakin Research Online (Deakin University) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
exercise training, skeletal muscle, lipid metabolism, fatty acid oxidation, fatty acid uptake, FAT/CD36, carnitine palmitoyltransferase 1, fatty acid beta-oxidation, PPARα, PPARγ, SREBP-1c, transcription factors, vastus lateralis, cycling exercise, gene expression
MeSH terms
Fatty Acid-Binding Protein 7, Adult, Carnitine O-Palmitoyltransferase, Carrier Proteins, DNA-Binding Proteins, Exercise Test, Female, Gene Expression Regulation, Humans, Male, Membrane Glycoproteins, Neoplasm Proteins, RNA, Messenger, Time Factors, Transcription Factors, 3-Hydroxyacyl CoA Dehydrogenases, Exercise, Receptors, Cytoplasmic and Nuclear, Muscle, Skeletal, CD36 Antigens, CCAAT-Enhancer-Binding Proteins, Tumor Suppressor Proteins, Organic Anion Transporters, Lipid Metabolism, Fatty Acid-Binding Proteins, Sterol Regulatory Element Binding Protein 1

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