Chronic probiotic supplementation with or without glutamine does not influence the eHsp72 response to a multi-day ultra-endurance exercise event
Open accessAbstract
Probiotic and glutamine supplementation increases tissue Hsp72, but their influence on extracellular Hsp72 (eHsp72) has not been investigated. The aim of this study was to investigate the effect of chronic probiotic supplementation, with or without glutamine, on eHsp72 concentration before and after an ultramarathon. Thirty-two participants were split into 3 independent groups, where they ingested probiotic capsules (PRO; n = 11), probiotic + glutamine powder (PGLn; n = 10), or no supplementation (CON; n = 11), over a 12-week period prior to commencement of the Marathon des Sables (MDS). eHsp72 concentration in the plasma was measured at baseline, 7 days pre-race, 6–8 h post-race, and 7 days post-race. The MDS increased eHsp72 concentrations by 124% (F[1,3] = 22.716, p < 0.001), but there was no difference in the response between groups. Additionally, PRO or PGLn supplementation did not modify pre- or post-MDS eHsp72 concentrations compared with CON (p > 0.05). In conclusion, the MDS caused a substantial increase in eHsp72 concentration, indicating high levels of systemic stress. However, chronic PRO or PGLn supplementation did not affect eHsp72 compared with control pre- or post-MDS. Given the role of eHsp72 in immune activation, the commercially available supplements used in this study are unlikely to influence this cascade.
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In 32 marathon competitors, a multi-strain probiotic for 12 weeks of marathon training showed no difference in marathon time to completion compared to control.
“Marshall et al. [81] investigated the effects of a multi-strain probiotic for 12 weeks of marathon training in a group of 32 marathon competitors and found no difference in marathon time to completion compared to the control group.”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [81]
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- Authors
- Marshall, H., Chrismas, B. C. R., Suckling, C. A., Roberts, J. D., Foster, J., & Taylor, L.
- Affiliations
- University of Bedfordshire (GB)
- Qatar University (QA)
- Anglia Ruskin University (GB)
- Loughborough University (GB)
- Venue
- Applied Physiology, Nutrition, and Metabolism
- Publisher
- Canadian Science Publishing
- Published
- 2017-08
- Volume
- 42
- Issue
- 8
- Pages
- 876-883
- ISSN
- 1715-5312 (ISSN-L)
- 1715-5320
- OpenAlex
- W2611385880
- Cited by
- 26
- References
- 59
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 6 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 1 · 2013: 1
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Applied Physiology Nutrition and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (acceptedVersion)
- Anglia Ruskin Research Online (Anglia Ruskin University) (submittedVersion)
- Loughborough University Institutional Repository (Loughborough University) (submittedVersion, cc-by-nc-nd)
- Loughborough University Research Repository (Loughborough University) (submittedVersion, cc-by-nc-nd)
- Research Portal (King's College London) (submittedVersion)
- Qatar University QSpace (Qatar University) (submittedVersion)
- University of Bedfordshire Repository (University of Bedfordshire) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- heat shock proteins, probiotic supplementation, glutamine supplementation, ultra-endurance exercise, ultramarathon, plasma biomarkers, exercise stress
- MeSH terms
- Adolescent, Adult, Anthropometry, Diet, Female, Glutamine, Humans, Male, Middle Aged, Oxygen Consumption, Physical Endurance, Surveys and Questionnaires, Running, Probiotics, HSP72 Heat-Shock Proteins, Young Adult
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