Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 12,201–12,300 of 48,846

A comparative study of wax ester digestion in fish
Patton, J. S., & Benson, A. A. (1975) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(75)90125-x · Cited by 53
Hypolipidemic activity of (−)‐hydroxycitrate
Sullivan, A. C., Triscari, J. M., Hamilton, J. G. C., & Ontko, J. A. (1977) · Lipids · doi:10.1007/bf02532964 · Cited by 53
Improvement in the quality of seed storage protein by transformation of Brassica napus with an antisense gene for cruciferin
Kohno‐Murase, J., Murase, M., Ichikawa, H., et al. (1995) · Theoretical and Applied Genetics · doi:10.1007/bf00223289 · Cited by 53
Lipid Activation of CTP:Phosphocholine Cytidylyltransferase α: Characterization and Identification of a Second Activation Domain
Lykidis, A., Jackson, P., & Jackowski, S. (2000) · Biochemistry · doi:10.1021/bi002140r · Cited by 53
Membrane perturbation and the mechanism of lipid-mediated transfer of DNA into cells
Mui, B. L., AHKONG, Q. F., Chow, L., & Hope, M. J. (2000) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(00)00226-1 · Cited by 53
Aerosolized Versus Instilled Exogenous Surfactant in a Nonuniform Pattern of Lung Injury
Lewis, J. F., & McCaig, L. (1993) · American Review of Respiratory Disease · doi:10.1164/ajrccm/148.5.1187 · Cited by 53
Biomarkers of Exposure and Effect in Flounder (Platichthys flesus) Exposed to Sediments of the Adriatic Sea
Viganò, L., Arillo, A., Falugi, C., et al. (2001) · Marine Pollution Bulletin · doi:10.1016/s0025-326x(01)00047-9 · Cited by 53
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate Cytidyltransferase in the Methanogenic ArchaeonMethanothermobacter thermoautotrophicus
Morii, H., Nishihara, M., & Koga, Y. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005925200 · Cited by 54
Effect of solvent adaptation on the antibiotic resistance in Pseudomonas putida S12
Isken, S., Santos, P. M., & de Bont, J. A. M. (1997) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051109 · Cited by 53
Genotype-phenotype relationship in various degrees of arylsulfatase A deficiency
Kappler, J., Leinekugel, P., Conzelmann, E., et al. (1991) · Human Genetics · doi:10.1007/bf00194634 · Cited by 53
Prostacyclin Release by Rat Cardiac Fibroblasts
Yu, H., Gallagher, A. M., Garfin, P. M., et al. (1997) · Hypertension · doi:10.1161/01.hyp.30.5.1047 · Cited by 53
Tumor Necrosis Factor-α Autoregulates Interleukin-6 Synthesis via Activation of Protein Kinase C
Kozawa, O., Suzuki, A., Kaida, T., Tokuda, H., & Uematsu, T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.40.25099 · Cited by 53
Lipid class and fatty acid distributions in particulate and dissolved fractions in the north Adriatic sea
Derieux, S., Fillaux, J., & Saliot, A. (1998) · Organic Geochemistry · doi:10.1016/s0146-6380(98)00089-8 · Cited by 53
High-frequency fluxes of labile compounds in the central Ligurian Sea, northwestern Mediterranean
Goutx, M., Momzikoff, A., Striby, L., Andersen, V., Marty, J., & Vescovali, I. (2000) · Deep Sea Research Part I Oceanographic Research Papers · doi:10.1016/s0967-0637(99)00101-6 · Cited by 52
Inhibitory Effects of Surfactant Protein A on Surfactant Phospholipid Hydrolysis by Secreted Phospholipases A2
Chabot, S., Koumanov, K. S., Lambeau, G. J., Gelb, M. H., Balloy, V., Chignard, M., Whitsett, J. A., & Touqui, L. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.2.995 · Cited by 53
Microbial growth and resuscitation alter community structure after perturbation
Haldeman, D. L., Amy, P. S., Ringelberg, D. B., et al. (1995) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.1995.tb00124.x · Cited by 53
Anaerobiosis induces complex changes in sterol esterification pattern in the yeast Saccharomyces cerevisiae
Valachovič, M., HronskÃ¡, L., & Hapala, I. (2001) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2001.tb10580.x · Cited by 56
Nuclear Localization of Enzymatically Active Green Fluorescent Protein-CTP:Phosphocholine Cytidylyltransferase α Fusion Protein Is Independent of Cell Cycle Conditions and Cell Types
DeLong, C. J., Qin, L., & Cui, Z. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m004644200 · Cited by 53
Cell Surface Display and Intracellular Trafficking of Free Glycosylphosphatidylinositols in Mammalian Cells
Baumann, N. A., Vidugirienė, J., Machamer, C. E., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.10.7378 · Cited by 53
Partitioning of 14c-labelled 2,2′,4,4′-tetrachlorobiphenyl between water and fish lipids
Ewald, G., & Larsson, P. (1994) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620131005 · Cited by 53
Immunocomplexes stimulate different signalling events to chemoattractants in the neutrophil and regulate L-selectin and beta 2-integrin expression differently.
Molad, Y., HAINES, K. A., Anderson, D. C., et al. (1994) · Biochemical Journal · doi:10.1042/bj2990881 · Cited by 53
Effect of lipids and organic solvents on the enzymic formation of zinc protoporphyrin and haem
Mazanowska, A., Neuberger, A., & TAIT, G. (1966) · Biochemical Journal · doi:10.1042/bj0980117 · Cited by 53
5-n-Alkylresorcinols from encysting Azotobacter vinelandii: isolation and characterization
Reusch, R. N., & Sadoff, H. L. (1979) · Journal of Bacteriology · doi:10.1128/jb.139.2.448-453.1979 · Cited by 53
Physical and chemical properties of microvitellogenin. A protein from the egg of the tobacco hornworm moth, Manduca sexta.
Kawooya, J. K., Osir, E. O., & Law, J. H. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67465-4 · Cited by 53
13-Hydroxyoctadecadienoic acid (13-HODE) stimulates prostacyclin production by endothelial cells
Setty, Y., Berger, M., & Stuart, M. J. (1987) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(87)90557-2 · Cited by 53
Interleukin-1β stimulates cytosolic phospholipase A2 in rheumatoid synovial fibroblasts
Hulkower, K. I., Hope, W. C., Chen, T., et al. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)90648-5 · Cited by 53
Importance of the different steps of glycosylation for the activity and secretion of lipoprotein lipase in rat preadipocytes studied with monensin and tunicamycin
Chajek‐Shaul, T., Friedman, G., Knobler, H. Y., et al. (1985) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(85)90235-8 · Cited by 53
Regulation of messenger ribonucleic acid expression of 1 alpha,25-dihydroxyvitamin D3-24-hydroxylase in rat osteoblasts.
Nishimura, A., Shinki, T., Jin, C., et al. (1994) · Endocrinology · doi:10.1210/endo.134.4.8137744 · Cited by 53
Safflower microsomes catalyse oil accumulation in vitro: A model system
Stobart, A. K., Stymne, S., & H�glund, S. (1986) · Planta · doi:10.1007/bf01369772 · Cited by 53
Lipids and fatty acids of a whitefish (Coregonus albula) flesh and roe
Kaitaranta, J. K. (1980) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740311213 · Cited by 53
Effect of dietary calcium and phosphorus on intestinal calcium absorption and vitamin D metabolism
Ribovich, M. L., & DeLuca, H. F. (1978) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(78)90367-3 · Cited by 53
The biosynthesis of linoleate and α-linolenate in homogenates from developing soya bean cotyledons
Stymne, S., & Appelqvist, L. (1980) · Plant Science Letters · doi:10.1016/0304-4211(80)90159-5 · Cited by 53
Sphingolipid-dependent fusion of Semliki Forest virus with cholesterol-containing liposomes requires both the 3-hydroxyl group and the double bond of the sphingolipid backbone
Corver, J., Moesby, L., Erukulla, R. K., et al. (1995) · Journal of Virology · doi:10.1128/jvi.69.5.3220-3223.1995 · Cited by 53
Distribution of apolipoprotein A-IV among lipoprotein subclasses in rat serum.
Dallinga‐Thie, G. M., Groot, P. H. E., & Arie van, T. O. L. (1985) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34300-5 · Cited by 53
Alterations in lipid metabolism induced by 12-O-tetradecanoylphorbol-13-acetate in differentiating human myeloid leukemia cells.
Cabot, M. C., Welsh, C. J., Callaham, M. F., et al. (1980) · PubMed · Cited by 53
Proteins of hepatitis B surface antigen
Shih, J. W., & Gerin, J. L. (1977) · Journal of Virology · doi:10.1128/jvi.21.1.347-357.1977 · Cited by 53
Purification and characterization of a lysophospholipase from a macrophage-like cell line P388D1.
Zhang, Y. Y., & Dennis, E. A. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)81612-5 · Cited by 53
Isolation and characterization of cells resistant to ML236B (compactin) with increased levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Ryan, J., Hardeman, E. C., Endo, A., et al. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69057-5 · Cited by 53
1,25-dihydroxyvitamin D3 receptors. Differential quantitation of endogenously occupied and unoccupied sites.
Hunziker, W. H., Walters, M. R., & Norman, A. W. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)43422-9 · Cited by 53
Purification and reconstitution of Escherichia coli proline carrier using a site specifically cleavable fusion protein.
Hanada, K., Yamato, I., & Anraku, Y. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68624-7 · Cited by 53
Heterogeneity of alveolar surfactant in the rabbit: composition, morphology, and labelling of subfractions isolated by centrifugation of lung lavage
Baritussio, A., Bellina, L., Carraro, R., et al. (1984) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.1984.tb00699.x · Cited by 53
Essential fatty acid deficiency ameliorates acute renal dysfunction in the rat after the administration of the aminonucleoside of puromycin.
Harris, K. P. G., Lefkowith, J. B., Klahr, S., & Schreiner, G. F. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114816 · Cited by 52
Phospholipid and Fatty Acid Characteristics of Erythrocytes in Some Cases of Anaemia
de Gier, J., Deenen, L. L. M. V., Verloop, M. C., et al. (1964) · British Journal of Haematology · doi:10.1111/j.1365-2141.1964.tb00699.x · Cited by 53
Phospholipid and Ca++ dependency of phorbol ester receptors
König, B., Nitto, P. A. D., & Blumberg, P. M. (1985) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240270307 · Cited by 53
Cholesterol Oxides in Commercial Dry Egg Products: Quantitation
TSAI, L. S., & Hudson, C. A. (1985) · Journal of Food Science · doi:10.1111/j.1365-2621.1985.tb13316.x · Cited by 53
Carbon-14 labelling patterns of individual marine phytoplankton from natural populations
Rivkin, R. B. (1985) · Marine Biology · doi:10.1007/bf00392884 · Cited by 53
31P-NMR studies on membrane phospholipids in microsomes, rat liver slices and intact perfused rat liver
KRUIJFF, B. D., Rietveld, A. G., & Cullis, P. R. (1980) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(80)90438-1 · Cited by 53
Surfactant Treatment Before Reperfusion Improves the Immediate Function of Lung Transplants in Rats
Erasmus, M. E., Petersen, A. H., Hofstede, G. J., et al. (1996) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.153.2.8564115 · Cited by 53
[8] Isolation and assay of dolichol and dolichyl phosphate
Adair, W. L., & Keller, R. K. (1985) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(85)11010-4 · Cited by 53
Phospholipase A Activity in the Hemolysis of Sheep and Human Erythrocytes by Rickettsia prowazeki
Winkler, H. H., & Miller, E. T. (1980) · Infection and Immunity · doi:10.1128/iai.29.2.316-321.1980 · Cited by 53
Phospholipase A1 of human blood platelets (Short Communication)
Smith, J. B., Silver, M. J., & Webster, G. R. (1973) · Biochemical Journal · doi:10.1042/bj1310615 · Cited by 53
Occurrence of an endogenous inhibitor of platelet-activating factor in rat liver.
Miwa, M., Hill, C. E., Kumar, R., et al. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75812-8 · Cited by 53
The biosynthesis of plasmalogens from labeled O-alkylglycerols in ehrlich ascites cells
Blank, M. L., Wykle, R. L., Piantadosi, C. A., et al. (1970) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(70)90040-8 · Cited by 53
Effect of sterol depletion on LM cell sterol mutants. Changes in the lipid composition of the plasma membrane and their effects on 3-O-methlglucose transport.
Baldassare, J. J., Saito, Y., & Silbert, D. F. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34174-1 · Cited by 53
Correction of Linoleic Acid Deficiency in Cystic Fibrosis
Mischler, E. H., Parrell, S. W., Farrell, P. M., et al. (1986) · Pediatric Research · doi:10.1203/00006450-198601000-00010 · Cited by 53
Retention of ellipticity between enzymatic states of the Ca2+‐ATPase of sarcoplasmic reticulum
Nakamoto, R. K., & Inesi, G. (1986) · FEBS Letters · doi:10.1016/0014-5793(86)80096-5 · Cited by 53
Bradykinin stimulates phosphodiesteratic cleavage of phosphatidylcholine in cultured endothelial cells
Martin, T. W., & Michaelis, K. C. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(88)81012-x · Cited by 53
Phospholipid molecular species alterations in microsomal membranes as an initial key step during cellular acclimation to low temperature
Dickens, B. F., & Thompson, G. A. (1982) · Biochemistry · doi:10.1021/bi00258a012 · Cited by 53
A direct fluorometric assay of benzo[a]pyrene hydroxylase
Yang, C. S., & Kicha, L. P. (1978) · Analytical Biochemistry · doi:10.1016/0003-2697(78)90494-3 · Cited by 53
Stress hormones mediated lipid accumulation and modulation of specific fatty acids in Nannochloropsis oceanica CASA CC201
Udayan, A., Sabapathy, H., & Arumugam, M. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.123437 · Cited by 51
Latest Trends and Developments in Microalgae as Potential Source for Biofuels: The Case of Diatoms
Mourya, M., Khan, M. J., Ahirwar, A., Schoefs, B., Marchand, J., Rai, A., Varjani, S., Rajendran, K., Banu, J. R., & Vinayak, V. (2022) · Fuel · doi:10.1016/j.fuel.2021.122738 · Cited by 52
Effective harvesting of microalgae by coagulation-flotation
Xia, L., Li, Y., Huang, R., & Song, S. (2017) · Royal Society Open Science · doi:10.1098/rsos.170867 · Cited by 53
Quantitative visualization of intracellular lipids concentration in a microalgae cell based on Raman micro-spectroscopy coupled with chemometrics
Li, X., Sha, J., Chu, B., Wei, Y., Huang, W., Zhou, H., Xu, N., & He, Y. (2019) · Sensors and Actuators B: Chemical · doi:10.1016/j.snb.2019.04.048 · Cited by 52
Enhancement of hepatic docosahexaenoic acid and arachidonic acid contents in C57BL/6J mice by dietary fucoxanthin
Tsukui, T., Baba, N., Hosokawa, M., Sashima, T., & Miyashita, K. (2009) · Fisheries Science · doi:10.1007/s12562-008-0018-4 · Cited by 52
Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development
Dutta, A., & Sinha, D. K. (2017) · Open Biology · doi:10.1098/rsob.170063 · Cited by 51
Thylakoid membrane lipid sulfoquinovosyl-diacylglycerol (SQDG) is required for full functioning of photosystem II in Thermosynechococcus elongatus
Nakajima, Y., Umena, Y., Nagao, R., et al. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.004304 · Cited by 52
Olive Oil, Palm Oil, and Hybrid Palm Oil Distinctly Modulate Liver Transcriptome and Induce NAFLD in Mice Fed a High-Fat Diet
Sales, R. C., Medeiros, P. D. C., Fernandes, F. S., de Velasco, P. C., Gonçalves, F., Martín‐Hernández, R., Mantilla-Escalante, D. C., Gil‐Zamorano, J., Peres, W. A. F., de Souza, S. A. L., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms20010008 · Cited by 52
Enhanced polyunsaturated fatty acid production using food wastes and biofuels byproducts by an evolved strain of Phaeodactylum tricornutum
Wang, X., Balamurugan, S., Liu, S.-F., et al. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.122351 · Cited by 52
Distinct regulation of microbial processes in the immobilization of labile carbon in different soils
Wang, X.-X., Zhang, W., Zhou, F., Liu, Y., He, H.-B., & Zhang, X.-D. (2020) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2020.107723 · Cited by 49
Interactions between substrate characteristics and microbial communities on biogas production yield and rate
Lee, J., Hong, J., Jeong, S., Chandran, K., & Park, K. Y. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.122934 · Cited by 55
Kitchen waste for economical amylase production using Bacillus amyloliquefaciens KCP2
Bhatt, B. M., Prajapati, V., Patel, K. C., et al. (2020) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2020.101654 · Cited by 52
Antithrombotic properties of Spirulina extracts against platelet-activating factor and thrombin
Koukouraki, P., Τσούπρας, Α., Sotiroudis, G., Demopoulos, C. A., & Sotiroudis, T. G. (2020) · Food Bioscience · doi:10.1016/j.fbio.2020.100686 · Cited by 52
Optimization and Comparison of Three Cell Disruption Processes on Lipid Extraction from Microalgae
Quesada-Salas, M. C., Delfau-Bonnet, G., Willig, G., Préat, N., Allais, F., & Ioannou, I. (2021) · Processes · doi:10.3390/pr9020369 · Cited by 54
“The total lipids extracted were measured from samples before and after disruption using a CHCl3: MeOH solvent extraction as second step [28].”
Method
Untargeted lipidomic approach in studying pinot noir wine lipids and predicting wine origin
Phan, Q., & Tomasino, E. (2021) · Food Chemistry · doi:10.1016/j.foodchem.2021.129409 · Cited by 52
Physiological and Biochemical Responses of Bicarbonate Supplementation on Biomass and Lipid Content of Green Algae Scenedesmus sp. BHU1 Isolated From Wastewater for Renewable Biofuel Feedstock
Singh, R. P., Yadav, P., Kumar, A., Hashem, A., Al-Arjani, A.-B. F., Abd_Allah, E. F., Rodríguez Dorantes, A., & Gupta, R. K. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.839800 · Cited by 53
“The Bligh and Dyer method was used to extract total lipids content from microalgae (Bligh and Dyer, 1959).”
Method
Understanding LC/MS‐Based Metabolomics: A Detailed Reference for Natural Product Analysis
Sarkar, J., Singh, R., & Chandel, S. (2024) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.202400048 · Cited by 52
“The ‘Folch’ and ‘Bligh and Dyer’ methods, recognized as the gold standard extraction procedures, utilize a chloroform/methanol combination in varying proportions and have established the basis for liquid–liquid extraction (LLE) [46, 47].”
Erythrocyte polyunsaturated fatty acid status, memory, cognition and mood in older adults with mild cognitive impairment and healthy controls
Milte, C. M., Parletta, N., Street, S. J., Buckley, J. D., Coates, A. M., & Howe, P. R. C. (2011) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/j.plefa.2011.02.002 · Cited by 51
The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition‐related alterations
Rühl, J., Hein, E., Hayen, H., Schmid, A., & Blank, L. M. (2011) · Microbial Biotechnology · doi:10.1111/j.1751-7915.2011.00286.x · Cited by 52
Variations in the diet of introduced Norway rats (Rattus norvegicus) inferred using stable isotope analysis
Major, H. L., Jones, I. L., Charette, M. R., et al. (2006) · Journal of Zoology · doi:10.1111/j.1469-7998.2006.00230.x · Cited by 52
Utilization of Scenedesmus obliquus biomass as feedstock for biodiesel and other industrially important co-products: An integrated paradigm for microalgal biorefinery
Patnaik, R., & Mallick, N. (2015) · Algal Research · doi:10.1016/j.algal.2015.09.009 · Cited by 52
Pro-oxidant/antioxidant behaviours of ascorbic acid, tocopherol, and plant extracts in n-3 highly unsaturated fatty acid rich oil-in-water emulsions
Jayasinghe, C. V. L., Gotoh, N., & Wada, S. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.05.143 · Cited by 52
Interactions between selected PAHs and the microbial community in rhizosphere of a paddy soil
SU, Y., & Yang, X. (2008) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2008.09.026 · Cited by 52
Inter-specific and geographical variations in the fatty acid composition of mangrove leaves: implications for using fatty acids as a taxonomic tool and tracers of organic matter
Meziane, T., Lee, S. Y., Mfilinge, P. L., Shin, P. K. S., Lam, M. H., & Tsuchiya, M. (2006) · Marine Biology · doi:10.1007/s00227-006-0424-z · Cited by 52
Antioxidant activity of hydroxytyrosol in frankfurters enriched with n-3 polyunsaturated fatty acids
Cofrades, S., Salcedo-Sandoval, L., Delgado‐Pando, G., et al. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2011.04.095 · Cited by 52
Fatty acid composition in wild and cultivated pacu and pintado fish
Tanamati, A., Stevanato, F. B., Visentainer, J. E. L., et al. (2009) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.200800103 · Cited by 52
An adaptive benefit of facultative coprophagy in the German cockroach Blattella germanica
Kopanic, R. J., Holbrook, G. L., Sevala, V. L., et al. (2001) · Ecological Entomology · doi:10.1046/j.1365-2311.2001.00316.x · Cited by 52
Plasma Phosphatidylcholine Alterations in Cystic Fibrosis Patients: Impaired Metabolism and Correlation with Lung Function and Inflammation
Grothe, J., Riethmüller, J., Tschürtz, S. M., et al. (2015) · Cellular Physiology and Biochemistry · doi:10.1159/000373964 · Cited by 52
Lipid hydrolysis and oxidation development in frozen mackerel (Scomber scombrus): Effect of a high hydrostatic pressure pre-treatment
Vázquez, M., Torres, J. A., Gallardo, J. M., et al. (2012) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2012.12.005 · Cited by 52
Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream
Kaneko, G., Yamada, T., Han, Y., Hirano, Y., Khieokhajonkhet, A., Shirakami, H., Nagasaka, R., Kondo, H., Hırono, I., Ushio, H., et al. (2013) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2013.01.003 · Cited by 52
20 Years of Fatty Acid Analysis by Capillary Electrophoresis
Oliveira, M. D., Porto, B. L. S., Faria, I. D. L., Oliveira, P. D., Barra, P. M. D. C., Castro, R., & Sato, R. T. (2014) · Molecules · doi:10.3390/molecules190914094 · Cited by 51
Screening and phylogenetic analysis of lipid-rich microalgae
Li, L., Cui, J., Liu, Q., Ding, Y., & Liu, J. (2015) · Algal Research · doi:10.1016/j.algal.2015.02.028 · Cited by 52
Optimization of the Matrix Solid Phase Dispersion Extraction Procedure for the Analysis of Polybrominated Diphenyl Ethers in Human Placenta
Dassanayake, R. M. A. P. S., Wei, H., Chen, R. C., et al. (2009) · Analytical Chemistry · doi:10.1021/ac901805d · Cited by 52
Novel approach on the risk assessment of oxidized fats and oils for perspectives of food safety and quality. I. Oxidized fats and oils induces neurotoxicity relating pica behavior and hypoactivity
Gotoh, N., Watanabe, H., Osato, R., INAGAKI, K., Iwasawa, A., & Wada, S. (2005) · Food and Chemical Toxicology · doi:10.1016/j.fct.2005.08.023 · Cited by 52
Transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH
Xu, X., Park, J., So, J., Hur, K. Y., & Lee, A. (2014) · Journal of Lipid Research · doi:10.1194/jlr.m045104 · Cited by 52
Different apoptosis ratios and gene expressions in two human cell lines after sevoflurane anaesthesia
Kvolik, S., Dobrošević, B., Marczi, S., et al. (2009) · Acta Anaesthesiologica Scandinavica · doi:10.1111/j.1399-6576.2009.02036.x · Cited by 52
Influence of Lipid Content and Lipoxygenase on Flavor Volatiles in the Tomato Peel and Flesh
Ties, P., & Barringer, S. A. (2012) · Journal of Food Science · doi:10.1111/j.1750-3841.2012.02775.x · Cited by 52
Biochemical composition of zooplankton community grown in freshwater earthen ponds: Nutritional implication in nursery rearing of fish larvae and early juveniles
Mitra, G., Mukhopadhyay, P. K., & Ayyappan, S. (2007) · Aquaculture · doi:10.1016/j.aquaculture.2007.08.026 · Cited by 52
Fish oil improves anxiety‐like, depressive‐like and cognitive behaviors in olfactory bulbectomised rats
Pudell, C., Vicente, B. A., Delattre, A. M., Carabelli, B., Mori, M., Suchecki, D., Machado, R. B., Zanata, S. M., Visentainer, J. V., Santos, O. D. O., et al. (2013) · European Journal of Neuroscience · doi:10.1111/ejn.12406 · Cited by 54
Macular Xanthophylls and ω-3 Long-Chain Polyunsaturated Fatty Acids in Age-Related Macular Degeneration
Arnold, C., Winter, L., Fröhlich, K., Jentsch, S., Dawczynski, J., Jahreis, G., & Böhm, V. (2013) · JAMA Ophthalmology · doi:10.1001/jamaophthalmol.2013.2851 · Cited by 52
Dietary trans‐Fatty Acid Induced NASH is Normalized Following Loss of trans‐Fatty Acids from Hepatic Lipid Pools
Neuschwander‐Tetri, B. A., Ford, D. A., Acharya, S., Gilkey, G. D., Başaranoğlu, M., Tetri, L. H., & Brunt, E. M. (2012) · Lipids · doi:10.1007/s11745-012-3709-7 · Cited by 56

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks