Quantitative visualization of intracellular lipids concentration in a microalgae cell based on Raman micro-spectroscopy coupled with chemometrics
Abstract
Abstract An in vivo, label-free, nondestructive, and quantitative visualization of the intracellular lipids concentration in individual microalgae cells under nitrogen stress was obtained by integrating Raman chemical imaging and multiple regression analysis. Lipids and biomass were significantly affected by nitrogen stress and induction time. Especially, in the first four days under stress, the higher the stress intensity, the faster the increase in the lipid concentration. A quantitative linear relationship between the Raman spectral response and the lipid concentration of colonial microalgae was established with satisfactory results (Rp2 = 0.925). Characteristic Raman bands for quantitative determination of lipids were then extracted based on competitive adaptive reweighted sampling (CARS). Furthermore, the visualization of intracellular lipid concentration derived from the quantitative linear regression model outperformed that by integration of Raman intensity at a fingerprint band (1266 or 1445 cm−1). This quantitative visualization of intracellular lipids concentration could effectively provide the spatial and temporal dynamic characteristics of intracellular lipids in microalgae cells under nitrogen stress, which should be helpful in revealing intracellular lipid metabolism and accumulation for the utilization of microalgae biomass in biofuel production.
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Li et al. investigated the effect of nitrogen stress on lipids concentration.
“The effect of nitrogen stress on lipids concentration was also investigated by Li et al. [90].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [90]
From the results, it was observed that faster lipid accumulation was achieved in the first four days under stress with increase in stress intensity.
“From the results, it was observed that faster lipid accumulation was achieved in the first four days under stress with increase in stress intensity.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [90]
Using this approach, a quantitative visualization of intracellular lipids concentration in microalgae cells was achieved, effectively providing spatial and temporal dynamic characteristics of the intracellular lipids of the microalgae cells under nitrogen stress.
“Using this approach, a quantitative visualization of intracellular lipids concentration in microalgae cells was achieved, effectively providing spatial and temporal dynamic characteristics of the intracellular lipids of the microalgae cells under nitrogen stress.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [90]
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Musa, M., Ayoko, G. A., Ward, A., Rösch, C., Brown, R. J., & Rainey, T. J. (2019) · Cells · doi:10.3390/cells8080851 · Cited by 97 · see citing works
- Authors
- Li, X., Sha, J., Chu, B., Wei, Y., Huang, W., Zhou, H., Xu, N., & He, Y.
- Affiliations
- State Key Laboratory of Modern Optical Instruments (CN)
- Zhejiang University of Technology (CN)
- Corresponding author
- He, Yong
- Venue
- Sensors and Actuators B: Chemical
- Publisher
- Elsevier BV
- Published
- 2019-08
- Volume
- 292
- Pages
- 7-15
- ISSN
- 0925-4005 (ISSN-L)
- OpenAlex
- W2938499088
- Semantic Scholar
- bdc1c45d43e65c45ef6f4100ee114c7c8eaed98b
- Cited by
- 52
- References
- 48
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 10 · 2023: 4 · 2022: 8 · 2021: 9 · 2020: 5 · 2019: 2
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Sensors and Actuators B Chemical (publishedVersion)
- Funders
- National Natural Science Foundation of China (31771676)
- National Key Research and Development Program of China (2018YFD0700500)
- Zhejiang Province Public Technology Research Program
- Special Funding Projects for Basic Scientific Research Projects in Universities
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- microalgae, nitrogen deficiency, Raman chemical imaging, label-free quantification, competitive adaptive reweighted sampling, lipid accumulation, lipid metabolism, biofuel production
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