Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 12,601–12,700 of 48,846

Reevaluation of the role of the multidrug-resistant P-glycoprotein in cellular cholesterol homeostasis
Goff, W. L., Settle, M., Greene, D. J., Morton, R. E., & Smith, J. D. (2005) · Journal of Lipid Research · doi:10.1194/jlr.m500255-jlr200 · Cited by 50
Lipid and protein deterioration during the chilled storage of minced sea salmon (Pseudopercis semifasciata)
Tironi, V. A., Tomás, M. C., & Añón, M. C. (2007) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2949 · Cited by 51
4GalT6 is involved in the synthesis of lactosylceramide with less intensity than 4GalT5
Tokuda, N., Numata, S., Li, X., Nomura, T., Takizawa, M., Kondo, Y., Yamashita, Y., Hashimoto, N., Kiyono, T., Urano, T., et al. (2013) · Glycobiology · doi:10.1093/glycob/cwt054 · Cited by 52
The impact of increased oxygen conditions on metal-contaminated sediments part II: Effects on metal accumulation and toxicity in aquatic invertebrates
Jonge, M. D., Teuchies, J., Meire, P. M., Blust, R., & Bervoets, L. (2012) · Water Research · doi:10.1016/j.watres.2012.03.035 · Cited by 50
The impact of diet on the growth and proximate composition of juvenile whelks, Dicathais orbita (Gastropoda: Mollusca)
Woodcock, S. H., & Benkendorff, K. (2008) · Aquaculture · doi:10.1016/j.aquaculture.2008.01.036 · Cited by 51
Lipid storage compounds in raw activated sludge microorganisms for biofuels and oleochemicals production
Revellame, E. D., Hernandez, R. A., French, W. T., et al. (2012) · RSC Advances · doi:10.1039/c2ra01078j · Cited by 51
Controls of microalgal biomass and lipid production in municipal wastewater‐fed bioreactors
Sturm, B., Peltier, E. F., Smith, V. H., & deNoyelles, F. J. (2011) · Environmental Progress & Sustainable Energy · doi:10.1002/ep.10586 · Cited by 51
A Phosphatidylinositol Species Acutely Generated by Activated Macrophages Regulates Innate Immune Responses
Gil‐de‐Gómez, L., Astudillo, A. M., Meana, C., et al. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1203494 · Cited by 51
A fish protein hydrolysate alters fatty acid composition in liver and adipose tissue and increases plasma carnitine levels in a mouse model of chronic inflammation
Bjørndal, B., Berge, C., Ramsvik, M. S., Svardal, A. M., Bohov, P., Skorve, J., & Berge, R. K. (2013) · Lipids in Health and Disease · doi:10.1186/1476-511x-12-143 · Cited by 51
Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity
Romanenko, L. A., Uchino, M., Frolova, G. M., et al. (2007) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.64441-0 · Cited by 51
Overexpression of Mitochondrial Sirtuins Alters Glycolysis and Mitochondrial Function in HEK293 Cells
de Moura, M. B., Uppala, R., Zhang, Y., Houten, B. V., & Goetzman, E. S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0106028 · Cited by 52
Pomegranate vinegar attenuates adiposity in obese rats through coordinated control of AMPK signaling in the liver and adipose tissue
Ok, E., Do, G.-M., Lim, Y., Park, J., Park, Y.-J., & Kwon, O. (2013) · Lipids in Health and Disease · doi:10.1186/1476-511x-12-163 · Cited by 52
Complementary transcriptomic, lipidomic, and targeted functional genetic analyses in cultured Drosophila cells highlight the role of glycerophospholipid metabolism in Flock House virus RNA replication
Castorena, K. M., Stapleford, K. A., & Miller, D. J. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-183 · Cited by 51
Peroxisome dependency of alkyl-containing GPI-anchor biosynthesis in the endoplasmic reticulum
Kanzawa, N., Maeda, Y., Ogiso, H., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0904762106 · Cited by 51
Arsenic trioxide induces accumulation of cytotoxic levels of ceramide in acute promyelocytic leukemia and adult T-cell leukemia/lymphoma cells through de novo ceramide synthesis and inhibition of glucosylceramide synthase activity
Dbaibo, G. S., Kfoury, Y. S., Darwiche, N., et al. (2007) · Haematologica · doi:10.3324/haematol.10968 · Cited by 51
Effects of sphingomyelin/ceramide ratio on the permeability and microstructure of model stratum corneum lipid membranes
Pullmannová, P., Staňková, K., Pospíšilová, M., Školová, B., Zbytovská, J., & Vávrová, K. (2014) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2014.05.001 · Cited by 51
Peanut skin extract reduces lipid oxidation in cooked chicken patties
Munekata, P. E. S., Calomeni, A. D. V., Rodrigues, C. E. D. C., Fávaro-Trindade, C. S., de Alencar, S. M., & Trindade, M. A. (2015) · Poultry Science · doi:10.3382/ps/pev005 · Cited by 51
Role of lysophosphatidic acid acyltransferase 3 for the supply of highly polyunsaturated fatty acids in TM4 Sertoli cells
Koeberle, A., Shindou, H., HARAYAMA, T., et al. (2010) · The FASEB Journal · doi:10.1096/fj.10-162818 · Cited by 51
Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata
Olofsson, M., Lamela, T., Nilsson, E., Bergé, J.-P., Pino, V. D., Uronen, P., & Legrand, C. (2014) · Marine Drugs · doi:10.3390/md12041891 · Cited by 49
Activation of the Liver X Receptor Prevents Lipopolysaccharide-induced Lung Injury
Gong, H., He, J., Lee, J. H., Mallick, E., Gao, X., Li, S., Homanics, G. E., & Xie, W. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.047753 · Cited by 53
A Functional Chitosan‐Enriched Fish Sausage Treated by High Pressure
López-Caballero, M. E., Góamez-Guillén, M. C., Pérez-Mateos, M., et al. (2005) · Journal of Food Science · doi:10.1111/j.1365-2621.2005.tb07145.x · Cited by 51
Growth, nutrition, and soil respiration of a mycorrhiza-defective tomato mutant and its mycorrhizal wild-type progenitor
Cavagnaro, T. R., Langley, A. J., Jackson, L. E., et al. (2008) · Functional Plant Biology · doi:10.1071/fp07281 · Cited by 51
Variation in vegetation and microbial linkages with slope aspect in a montane temperate hardwood forest
Gilliam, F. S., Hédl, R., Chudomelová, M., McCulley, R. L., & Nelson, J. A. (2014) · Ecosphere · doi:10.1890/es13-00379.1 · Cited by 49
Inhibition of serine palmitoyltransferase reduces Aβ and tau hyperphosphorylation in a murine model: a safe therapeutic strategy for Alzheimer's disease
Geekiyanage, H., Upadhye, A., & Chan, C. (2013) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2013.02.001 · Cited by 52
Accumulation of Phosphatidic Acid Increases Vancomycin Resistance in Escherichia coli
Sutterlin, H. A., Zhang, S., & Silhavy, T. J. (2014) · Journal of Bacteriology · doi:10.1128/jb.01876-14 · Cited by 51
Porphyromonas gingivalis Lipids Inhibit Osteoblastic Differentiation and Function
Wang, Y., Jiang, J., Zhu, Q., et al. (2010) · Infection and Immunity · doi:10.1128/iai.00225-10 · Cited by 51
Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red AlgaCyanidioschyzon merolae
Sumiya, N., Kawase, Y., Hayakawa, J., Matsuda, M., Nakamura, M., Era, A., Tanaka, K., Kondo, A., Hasunuma, T., Imamura, S., et al. (2015) · Plant and Cell Physiology · doi:10.1093/pcp/pcv120 · Cited by 50
Vinyl Chloride Metabolites Potentiate Inflammatory Liver Injury Caused by LPS in Mice
Anders, L. C., Lang, A. L., Anwar‐Mohamed, A., et al. (2016) · Toxicological Sciences · doi:10.1093/toxsci/kfw045 · Cited by 51
Functional analysis of two isoforms of phosphatidylethanolamine N-methyltransferase
Morita, S., Takeuchi, A., & Kitagawa, S. (2010) · Biochemical Journal · doi:10.1042/bj20100490 · Cited by 51
Heating effects on physicochemical characteristics and antioxidant activity of flaxseed hull oil (Linum usitatissimum L)
Herchi, W., Ammar, K. B., Bouali, I., et al. (2016) · Food Science and Technology · doi:10.1590/1678-457x.0109 · Cited by 51
Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density
Polburee, P., Yongmanitchai, W., Honda, K., et al. (2016) · Biochemical Engineering Journal · doi:10.1016/j.bej.2016.04.024 · Cited by 51
ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells
Tuohetahuntila, M., Molenaar, M. R., Spee, B., Brouwers, J. F. H. M., Houweling, M., Vaandrager, A. B., & Helms, J. B. (2016) · Journal of Lipid Research · doi:10.1194/jlr.m066415 · Cited by 53
Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues
Liaw, L., Prudovsky, I. A., Koza, R. A., Anunciado-Koza, R. V. P., Siviski, M. E., Lindner, V., Friesel, R. E., Rosen, C. J., Baker, P. R. S., Simons, B. L., et al. (2016) · Journal of Cellular Biochemistry · doi:10.1002/jcb.25522 · Cited by 51
The biochemical composition of Enteromorpha spp. from the Gulf of Gdansk coast on the southern Baltic Sea
Haroon, A. M., Szaniawska, A., Normant, M., & Janas, U. (2000) · Oceanologia · Cited by 53
Cardiac cytochrome c and cardiolipin depletion during anthracycline-induced chronic depression of mitochondrial function
Pereira, G. C., Pereira, S. P., Tavares, L. C., Carvalho, F. S., Novais, S. M., Barbosa, I. A., Santos, M. S., Bjork, J. A., Moreno, A. J., Wallace, K. B., et al. (2016) · Mitochondrion · doi:10.1016/j.mito.2016.07.005 · Cited by 51
Reduced nitrite and biogenic amine concentrations and improved flavor components of Chinese sauerkraut via co-culture of Lactobacillus plantarum and Zygosaccharomyces rouxii
Wu, C., Zheng, J., Huang, J., & Zhou, R. (2013) · Annals of Microbiology · doi:10.1007/s13213-013-0724-8 · Cited by 52
Isolation and Analysis of Phospholipids in Dairy Foods
Pimentel, L. L., Gomes, A., Pintado, M., & Rodríguez-Alcalá, L. M. (2016) · Journal of Analytical Methods in Chemistry · doi:10.1155/2016/9827369 · Cited by 53
Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption
Bates, M. A., Brandenberger, C., Langohr, I. M., Kumagai, K., Lock, A. L., Harkema, J. R., Holian, A., & Pestka, J. J. (2016) · PLoS ONE · doi:10.1371/journal.pone.0160622 · Cited by 53
Ectopic expression of AtDGAT1, encoding diacylglycerol O-acyltransferase exclusively committed to TAG biosynthesis, enhances oil accumulation in seeds and leaves of Jatropha
Maravi, D. K., Kumar, S., Sharma, P. K., Kobayashi, Y., Goud, V. V., Sakurai, N., Koyama, H., & Sahoo, L. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0642-7 · Cited by 50
Wnt Ligands Differentially Regulate Toxicity and Translocation of Graphene Oxide through Different Mechanisms in Caenorhabditis elegans
Zhi, L., Ren, M., Qu, M., et al. (2016) · Scientific Reports · doi:10.1038/srep39261 · Cited by 51
Quercetin improves macrophage reverse cholesterol transport in apolipoprotein E-deficient mice fed a high-fat diet
Cui, Y., Hou, P., Li, F., Liu, Q., Qin, S., Zhou, G., Xu, X., Si, Y., & Guo, S. (2017) · Lipids in Health and Disease · doi:10.1186/s12944-016-0393-2 · Cited by 51
Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production
Cobos, M., Paredes, J. D., Maddox, J. D., Vargas-Arana, G., Ramos, L. F., Aguilar, C., Marapara, J. L., & Castro, J. C. (2017) · Energies · doi:10.3390/en10020224 · Cited by 55
Purity and yield of collagen extracted from southern catfish ( Silurus meridionalis Chen) skin through improved pretreatment methods
Xu, S., Yang, H., Shen, L., & Li, G. (2017) · International Journal of Food Properties · doi:10.1080/10942912.2017.1291677 · Cited by 51
Selective enrichment of Eicosapentaenoic acid (20:5n-3) in N. oceanica CASA CC201 by natural auxin supplementation
Udayan, A., & Arumugam, M. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.03.149 · Cited by 51
Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum
Zhu, B., Tu, C., Shi, H.-P., Yang, G., & Pan, K. (2017) · Process Biochemistry · doi:10.1016/j.procbio.2017.03.013 · Cited by 52
Yeast PAH1-encoded phosphatidate phosphatase controls the expression of CHO1-encoded phosphatidylserine synthase for membrane phospholipid synthesis
Han, G., & Carman, G. M. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.801720 · Cited by 51
Bioconversion of biodiesel-derived crude glycerol into lipids and carotenoids by an oleaginous red yeast Sporidiobolus pararoseus KM281507 in an airlift bioreactor
Manowattana, A., Techapun, C., Watanabe, M., & Chaiyaso, T. (2017) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2017.07.014 · Cited by 52
Specific Physical Exercise Improves Energetic Metabolism in the Skeletal Muscle of Amyotrophic-Lateral- Sclerosis Mice
Desseille, C., Deforges, S., Biondi, O., Houdebine, L., D’amico, D., Lamazière, A., Caradeuc, C., Bertho, G., Bruneteau, G., Weill, L., et al. (2017) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2017.00332 · Cited by 50
Lipidomics Analysis of Behavioral Variant Frontotemporal Dementia: A Scope for Biomarker Development
Kim, W. S., Jary, E., Pickford, R., He, Y., Ahmed, R. M., Piguet, O., Hodges, J. R., & Halliday, G. M. (2018) · Frontiers in Neurology · doi:10.3389/fneur.2018.00104 · Cited by 50
Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies
Slatter, D. A., Percy, C. L., Allen‐Redpath, K., Gajsiewicz, J. M., Brooks, N. J., Clayton, A., Tyrrell, V. J., Rosas, M., Lauder, S. N., Watson, A., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.98459 · Cited by 50
Subventricular zone lipidomic architecture loss in Huntington's disease
Hunter, M., Demarais, N. J., Faull, R. L. M., Grey, A. C., & Curtis, M. A. (2018) · Journal of Neurochemistry · doi:10.1111/jnc.14468 · Cited by 53
GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase
Ishikawa, T., Fang, L., Rennie, E. A., et al. (2018) · PLANT PHYSIOLOGY · doi:10.1104/pp.18.00396 · Cited by 51
Hypocholesterolemic effect of Spirulina platensis (SP) fortified functional soy yogurts on diet-induced hypercholesterolemia
Sengupta, S., Koley, H., Dutta, S., & Bhowal, J. (2018) · Journal of Functional Foods · doi:10.1016/j.jff.2018.07.007 · Cited by 51
Processing techniques of selected oilseed by-products of potential use in animal feed: Effects on proximate nutrient composition, amino acid profile and antinutrients
Duodu, C. P., Adjei‐Boateng, D., Edziyie, R. E., et al. (2018) · Animal nutrition · doi:10.1016/j.aninu.2018.05.007 · Cited by 51
Effects of nano-sizing on lipid bioaccessibility and ex vivo bioavailability from EPA-DHA rich oil in water nanoemulsion
Dey, T. K., Koley, H., Ghosh, M., Dey, S., & Dhar, P. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.09.084 · Cited by 52
miR-107 inhibits CDK6 expression, differentiation, and lipid storage in human adipocytes
Ahonen, M. A., Haridas, P. A. N., Mysore, R., Wabitsch, M., Fischer‐Posovszky, P., & Olkkonen, V. M. (2018) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2018.09.007 · Cited by 51
Comprehensive and Reproducible Untargeted Lipidomic Workflow Using LC-QTOF Validated for Human Plasma Analysis
Forest, A., Ruiz, M., Bouchard, B., Boucher, G., Gingras, O., Daneault, C., Frayne, I. R., Rhainds, D., The iGenoMed Consortium, The NIDDK IBD Genetics Consortium, et al. (2018) · Journal of Proteome Research · doi:10.1021/acs.jproteome.8b00270 · Cited by 52
A potential role for lysophosphatidylcholine in the delivery of long chain polyunsaturated fatty acids to the fetal circulation
Ferchaud‐Roucher, V., Kramer, A. W., Silva, E., et al. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2018.12.007 · Cited by 51
Integrated strategy for the extraction and profiling of bioactive metabolites from Passiflora mollissima seeds combining pressurized-liquid extraction and gas/liquid chromatography–high resolution mass spectrometry
Ballesteros-Vivas, D., Álvarez‐Rivera, G., Ibáñez, E., Parada‐Alfonso, F., & Cifuentes, A. (2019) · Journal of Chromatography A · doi:10.1016/j.chroma.2019.02.031 · Cited by 51
Ceramide Suppresses Influenza A Virus Replication In Vitro
Soudani, N., Hage‐Sleiman, R., Karam, W. G., et al. (2019) · Journal of Virology · doi:10.1128/jvi.00053-19 · Cited by 51
Extramitochondrial cardiolipin suggests a novel function of mitochondria in spermatogenesis
Ren, M., Xu, Y., Erdjument‐Bromage, H., Donelian, A., Phoon, C. K. L., Terada, N., Strathdee, D., Neubert, T. A., & Schlame, M. (2019) · The Journal of Cell Biology · doi:10.1083/jcb.201808131 · Cited by 51
Quality Changes of Fish Burger from Rainbow Trout During Refrigerated Storage.
Taşkaya, L., Kışla, Ş. Ç. D., & Kılınç, B. (2003) · DergiPark (Istanbul University) · Cited by 51
Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis
Brenac, L., Baidoo, E. E. K., Keasling, J. D., & Budin, I. (2019) · Molecular Microbiology · doi:10.1111/mmi.14380 · Cited by 46
The function of nanoemulsion on preservation of rainbow trout fillet
Durmuş, M., Özoğul, Y., Köşker, A. R., Uçar, Y., Küley, E., Ceylan, Z., & Özoğul, F. (2019) · Journal of Food Science and Technology · doi:10.1007/s13197-019-04122-9 · Cited by 53
Meat Quality of Commercial Chickens Reared in Different Production Systems: Industrial, Range and Organic
Gálvez, F., Domínguez, R., Maggiolino, A., et al. (2019) · Annals of Animal Science · doi:10.2478/aoas-2019-0067 · Cited by 51
Universal phase behaviors of intracellular lipid droplets
Shimobayashi, S. F., & Ohsaki, Y. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1916248116 · Cited by 49
Sphingolipid Signature of Human Feto-Placental Vasculature in Preeclampsia
Gaudio, I. D., Sasset, L., Lorenzo, A. D., & Wadsack, C. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031019 · Cited by 50
Rocket Science: The Effect of Spaceflight on Germination Physiology, Ageing, and Transcriptome of Eruca sativa Seeds
Chandler, J. O., Haas, F. B., Khan, S., Bowden, L., Ignatz, M., Enfissi, E. M. A., Gawthrop, F., Griffiths, A., Fraser, P. D., Rensing, S. A., et al. (2020) · Life · doi:10.3390/life10040049 · Cited by 49
“This extraction method is adapted from the classic Bligh and Dyer method [55].”
Method
Osh6 requires Ist2 for localization to ER–PM contacts and efficient phosphatidylserine transport in budding yeast
D’Ambrosio, J. M., Albanèse, V., Lipp, N.-F., Fleuriot, L., Debayle, D., Drin, G., & Čopič, A. (2020) · Journal of Cell Science · doi:10.1242/jcs.243733 · Cited by 50
Nutritional and prebiotic efficacy of the microalga Arthrospira platensis (spirulina) in honey bees
Ricigliano, V. A., & Simone-Finstrom, M. D. (2020) · Apidologie · doi:10.1007/s13592-020-00770-5 · Cited by 54
“Modified Bligh and Dyer extractions (Bligh and Dyer 1959) were performed with EquiSPLASH™ Lipidomix™, and Lipidyzer™ standards (Avanti Polar Lipids), and different chain lengths of phosphatidylglycerol, phosphatidylserine, and phosphatidylinositol (Avanti Polar Lipids) were added as internal…”
Method
Encapsulation of fish oil by carrageenan and gum tragacanth as wall materials and its application to the enrichment of chicken nuggets
Pourashouri, P., Shábanpour, B., Heydari, S., et al. (2020) · LWT · doi:10.1016/j.lwt.2020.110334 · Cited by 51
Release of Anti-Inflammatory Palmitoleic Acid and Its Positional Isomers by Mouse Peritoneal Macrophages
Astudillo, A. M., Meana, C., Arias, M. Á. B., Pérez‐Encabo, A., Balboa, M. A., & Balsinde, J. (2020) · Biomedicines · doi:10.3390/biomedicines8110480 · Cited by 51
“Gas Chromatography/Mass Spectrometry (GC/MS) Analyses Total lipids from approximately 107 cells were extracted according to Bligh and Dyer [32], and internal standards were added.”
MethodInfluential
Valorization of Seaweed Carbohydrates: Autohydrolysis as a Selective and Sustainable Pretreatment
Gomes-Dias, J. S., Romaní, A., Teixeira, J. A., & Rocha, C. M. R. (2020) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.0c05396 · Cited by 51
Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α
Mann, S. N., Hadad, N., Holte, M. N., Rothman, A. R., Sathiaseelan, R., Mondal, S. A., Agbaga, M., Unnikrishnan, A., Subramaniam, M., Hawse, J. R., et al. (2020) · eLife · doi:10.7554/elife.59616 · Cited by 52
Comparison the functional properties of protein Hydrolysates from poultry byproducts and rainbow trout
Taheri, A., Anvar, S. A. A., Ahari, H., & Fogliano, V. (2013) · AquaDocs (United Nations Educational, Scientific and Cultural Organization) · Cited by 51
Development, properties, and stability of antioxidant shrimp muscle protein films incorporating carotenoid-containing extracts from food by-products
Gómez‐Estaca, J., Calvo, M. M., Sánchez-Faure, A., Montero, P., & Gómez-Guillén, M. C. (2015) · LWT · doi:10.1016/j.lwt.2015.05.052 · Cited by 52
Turmeric dye extraction residue for use in bioactive film production: Optimization of turmeric film plasticized with glycerol
Maniglia, B. C., de Paula, R. L., Domingos, J. R., et al. (2015) · LWT · doi:10.1016/j.lwt.2015.07.025 · Cited by 51
Influenza Virosomes in Vaccine Development
Huckriede, A. L. W., Bungener, L. B., Daemen, T., & Wilschut, J. (2003) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(03)73005-5 · Cited by 51
[33] Assessment of prooxidant activity of vitamin E in human low-density lipoprotein and plasma
Witting, P. K., Mohr, D., & Stocker, R. (1999) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(99)99036-5 · Cited by 51
Sterol metabolism in the oomycete Aphanomyces euteiches, a legume root pathogen
Madoui, M., Bertrand‐Michel, J., Gaulin, E., & Dumas, B. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2009.02895.x · Cited by 52
Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes
van Klompenburg, W., Paetzel, M., de Jong, J. M., et al. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00733-9 · Cited by 51
Lipidomic analysis of Toxoplasma gondii tachyzoites rhoptries: further insights into the role of cholesterol
Besteiro, S., Bertrand‐Michel, J., Lebrun, M., et al. (2008) · Biochemical Journal · doi:10.1042/bj20080795 · Cited by 51
Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities
Ghosal, A., Banaś, A., Ståhl, U., et al. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2007.10.007 · Cited by 51
Biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum . I: The use of externally supplied fatty acids
Shiran, D., Khozin‐Goldberg, I., Heimer, Y. M., et al. (1996) · Lipids · doi:10.1007/bf02587913 · Cited by 51
Chemopreventive effects of dietary mustard oil on colon tumor development
Dwivedi, C., Muller, L. A., Goetz-Parten, D. E., et al. (2003) · Cancer Letters · doi:10.1016/s0304-3835(03)00211-8 · Cited by 51
Microscale recovery of total lipids from fish tissue by accelerated solvent extraction
Dodds, E. D., McCoy, M. R., Geldenhuys, A., et al. (2004) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-004-0988-2 · Cited by 51
Lipoproteins of the egg perivitelline fluid ofPomacea canaliculata snails (Mollusca: Gastropoda)
Garin, C. F., Heras, H., & Pollero, R. J. (1996) · Journal of Experimental Zoology · doi:10.1002/(sici)1097-010x(19961201)276:5 · Cited by 51
Impact of Thermal Processing on the Activity of Gallotannins and Condensed Tannins from Hamamelis virginiana Used as Functional Ingredients in Seafood
González, M. J., Torres, J. L., & Medina, I. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf904032y · Cited by 51
Temporal variation of lysosomal capacities in relation to susceptibility of mussels, Mytilus edulis , to summer mortality
Tremblay, R., Myrand, B., & Guderley, H. (1998) · Marine Biology · doi:10.1007/s002270050429 · Cited by 51
Composition and oligosaccharides of a milk sample of the giant panda, Ailuropoda melanoleuca
Nakamura, T., Urashima, T., Mizukami, T., et al. (2003) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/s1096-4959(03)00093-9 · Cited by 51
Mercury in Arctic char (Salvelinus alpinus) populations from Greenland
Rigét, F. F., Asmund, G., & Aastrup, P. (2000) · The Science of The Total Environment · doi:10.1016/s0048-9697(99)00441-6 · Cited by 51
Biologically active polypeptides from the tropical sea anemone Radianthus macrodactylus
Monastyrnaya, M., Zykova, T. A., Apalikova, O. V., et al. (2002) · Toxicon · doi:10.1016/s0041-0101(02)00139-3 · Cited by 51
Cholesterol mobilization and regression of atheroma in cholesterol-fed rabbits induced by large unilamellar vesicles
Rodrigueza, W. V., Klimuk, S. K., Pritchard, P. H., & Hope, M. J. (1998) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(97)00198-3 · Cited by 51
Separation of Biologically Active Lipids from Red Wine
Fragopoulou, E., Nomikos, T., Antonopoulou, S., et al. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf990554p · Cited by 51
Changes in lipid composition during in vitro encystation and fatty acid desaturase activity of Giardia lamblia
Ellis, J. E., Wyder, M. A., Jarroll, E. L., et al. (1996) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(96)02677-1 · Cited by 51
Bioaccumulation of PCB congeners in marine benthic infauna
Magnusson, K., Ekelund, R., Grabic, R., et al. (2006) · Marine Environmental Research · doi:10.1016/j.marenvres.2005.11.004 · Cited by 51
Fractionation of livers following diosgenin treatment to elevate biliary cholesterol
Román, I. D., THEWLES, A., & COLEMAN, R. (1995) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(94)00212-h · Cited by 51
Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.
Krishnamurthi, S., Ruckmani, A., Pukall, R., et al. (2010) · Systematic and Applied Microbiology · doi:10.1016/j.syapm.2010.06.003 · Cited by 51
Metal‐lon stimulation and inhibition of lysophospholipase D which generates bioactive lysophosphatidic acid in rat plasma
Tokumura, A., Miyake, M., Yoshimoto, O., et al. (1998) · Lipids · doi:10.1007/s11745-998-0299-2 · Cited by 51
Archaeosomes with encapsulated antigens for oral vaccine delivery
Li, Z., Zhang, L., Sun, W., Ding, Q., Hou, Y., & Xu, Y. (2011) · Vaccine · doi:10.1016/j.vaccine.2011.05.015 · Cited by 51

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks