Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 13,001–13,100 of 48,846

Protein D, the immunoglobulin D-binding protein of Haemophilus influenzae, is a lipoprotein
Janson, H., Hedén, L. O., & Forsgren, A. (1992) · Infection and Immunity · doi:10.1128/iai.60.4.1336-1342.1992 · Cited by 50
The Stress-Responsive dgk Gene from Streptococcus mutans Encodes a Putative Undecaprenol Kinase Activity
Lis, M., & Kuramitsu, H. K. (2003) · Infection and Immunity · doi:10.1128/iai.71.4.1938-1943.2003 · Cited by 50
Carcass characteristics and chemical composition of the Longissimus muscle of crossbred bulls (Bos taurus indicus vs Bos taurus taurus) finished in feedlot
do Prado, I. N., do Prado, R. M., Rotta, P. P., et al. (2008) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/66609/2008 · Cited by 50
The Bryostatins
Pettit, G. R. (1991) · Fortschritte der Chemie Organischer Naturstoffe/Fortschritte der Chemie organischer Naturstoffe/Progress in the chemistry of organic natural products · doi:10.1007/978-3-7091-9119-4_3 · Cited by 50
Evidence for cis-trans isomerization of a double bond in the fatty acids of the psychrophilic bacterium Vibrio sp. strain ABE-1
Morita, N., SHIBAHARA, A., Yamamoto, K., Shinkai, K., KAJIMOTO, G., & Okuyama, H. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.3.916-918.1993 · Cited by 50
A trans-unsaturated fatty acid in a psychrophilic bacterium, Vibrio sp. strain ABE-1
Okuyama, H., Sasaki, S., Higashi, S., et al. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.6.3515-3518.1990 · Cited by 50
Vascular smooth-muscle cells contain AT1 angiotensin receptors coupled to phospholipase D activation
Freeman, E. J., & Tallant, E. A. (1994) · Biochemical Journal · doi:10.1042/bj3040543 · Cited by 50
The estimation of vitamin D and some metabolites in human plasma by mass fragmentography
Seamark, D., Trafford, D. J. H., & Makin, H. L. J. (1980) · Clinica Chimica Acta · doi:10.1016/0009-8981(80)90374-5 · Cited by 50
Biochemical composition of the blue crab Callinectes sapidus exposed to the water-soluble fraction of crude oil
Wang, S. Y., & Stickle, W. B. (1988) · Marine Biology · doi:10.1007/bf00392655 · Cited by 50
A Standard Reference Diet for Crustacean Nutrition Research. I. Evaluation of Two Formulations
Castell, J. D., Kean, J. C., D’Abramo, L. R., et al. (1989) · Journal of the World Aquaculture Society · doi:10.1111/j.1749-7345.1989.tb00560.x · Cited by 50
Purification and properties of a very high density lipoprotein from the hemolymph of the honeybee Apis mellifera
Shipman, B. A., Ryan, R. O., Schmidt, J. O., et al. (1987) · Biochemistry · doi:10.1021/bi00381a015 · Cited by 50
ω3‐Fatty Acids in Some Fish Species from Turkey
Sağlık, S., & İmre, S. (2001) · Journal of Food Science · doi:10.1111/j.1365-2621.2001.tb11318.x · Cited by 50
Inhibitors of CoA-independent transacylase block the movement of arachidonate into 1-ether-linked phospholipids of human neutrophils
Chilton, F. H., Fonteh, A. N., Sung, C.-M., Hickey, D. M. B., Torphy, T. J., Mayer, R. J., Marshall, L. A., Heravi, J., & Winkler, J. D. (1995) · Biochemistry · doi:10.1021/bi00016a011 · Cited by 50
The role of Mg2+-dependent phosphatidate phosphohydrolase in pulmonary glycerolipid biosynthesis
Walton, P. A., & Possmayer, F. (1984) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(84)90139-5 · Cited by 50
Comparison of three lipid extraction methods for fish
Honeycutt, M., McFarland, V., & McCant, D. (1995) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/bf00206688 · Cited by 50
Biosynthesis of cardiolipin in mitochondria isolated from guinea pig liver
Davidson, J. B., & Stanaćev, N. Ž. (1971) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(71)90032-5 · Cited by 50
13-Hydroxyoctadeca-9,11-dienoic acid (13-HODE) inhibits thromboxane A2 synthesis, and stimulates 12-HETE production in human platelets
Setty, Y., Berger, M., & Stuart, M. J. (1987) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(87)90908-9 · Cited by 50
Glucocorticoid stimulation of fatty acid synthesis in explants of human fetal lung
Gonzales, L. W., Ertsey, R. D., Ballard, P. L., Froh, D. K., Goerke, J., & Gonzales, J. (1990) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(90)90049-4 · Cited by 50
Arachidonic acid production byMortierella sp. S-17 influence of C/N ratio
Šajbidor, J., Dobroñová, S., & Čertík, M. (1990) · Biotechnology Letters · doi:10.1007/bf01024404 · Cited by 50
Changes in membrane fatty acids and delta-9 desaturase in senescence accelerated (SAMP8) mouse hippocampus with aging
Kumar, V. B., Vyas, K., Buddhiraju, M., et al. (1999) · Life Sciences · doi:10.1016/s0024-3205(99)00414-2 · Cited by 50
Fractionation ofPneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations
Stefano, J. A. D., Foy, J., Sullivan, D. W., Dawes, S. M., Cushion, M. T., Babcock, G. F., Sleight, R. G., van HALBEEK, H., & Walzer, P. D. (1994) · Parasitology Research · doi:10.1007/bf00932616 · Cited by 50
N–3 fatty acid deficiency in the rat pineal gland: effects on phospholipid molecular species composition and endogenous levels of melatonin and lipoxygenase products
Zhang, H., Hamilton, J., Salem, N., & Kim, H. (1998) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32520-7 · Cited by 48
Maintenance of Differentiated Function of the Surfactant System in Human Fetal Lung Type II Epithelial Cells Cultured on Plastic
Gonzales, L. W., Angampalli, S., Guttentag, S. H., et al. (2001) · Fetal and Pediatric Pathology · doi:10.1080/15513810109168622 · Cited by 50
Fatty acid distribution and lipid metabolism in developing seeds of laurate-producing rape ( Brassica napus L.)
Wiberg, E., Banaś, A., & Stymne, S. (1997) · Planta · doi:10.1007/s004250050200 · Cited by 50
Characterization of the mechanisms involved in the gender differences in hepatic taurocholate uptake
Simon, F. R., Fortune, J., Iwahashi, M., et al. (1999) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1999.276.2.g556 · Cited by 50
Characterization of a specific, high affinity binding macromolecule for 1 alpha, 25-dihydroxyvitamin D3 in cultured chick kidney cells
Simpson, R. U., Franceschi, R. T., & DeLuca, H. F. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70442-6 · Cited by 50
Detergent inactivation of sodium- and potassium-activated adenosinetriphosphatase of the electric eel
Brotherus, J. R., Jost, P. C., Griffith, O. H., et al. (1979) · Biochemistry · doi:10.1021/bi00590a003 · Cited by 50
Diacylglycerol-transporting lipoproteins and flight in Locusta
Mwangi, R. W., & Goldsworthy, G. J. (1981) · Journal of Insect Physiology · doi:10.1016/0022-1910(81)90031-7 · Cited by 50
The metabolism of lyso-platelet-activating factor (1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine) by a calcium-dependent lysophospholipase D in rabbit kidney medulla
Kawasaki, T., & Snyder, F. F. C. (1987) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(87)90314-6 · Cited by 50
Reversible envelope effects during and after killing of Escherichia coli W by a highly-purified rabbit polymorphonuclear leukocyte fraction
Weiss, J. P., Franson, R. C., Schmeidler, K., et al. (1976) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(76)90227-3 · Cited by 50
Bile acid and lysolecithin concentrations in the stomach in patients with duodenal ulcer before operation and after treatment by highly selective vagotomy, partial gastrectomy, or truncal vagotomy and drainage
Dewar, P., King, R., & Johnston, D. (1982) · Gut · doi:10.1136/gut.23.7.569 · Cited by 49
Interaction of erythrocyte protein 4.1 with phospholipids. A monolayer and liposome study
Shiffer, K. A., Goerke, J., Düzgüneş, N., et al. (1988) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(88)90249-0 · Cited by 50
Structure and dynamic properties of dehydroergosterol, 13-113-113-1
Fischer, R. T., Stephenson, F. A., Shafiee, A. R., et al. (1985) · Journal of Biological Physics · doi:10.1007/bf01872877 · Cited by 50
Synthesis of trans unsaturated fatty acids in Pseudomonas putida P8 by direct isomerization of the double bond of lipids
Diefenbach, R., & Keweloh, H. (1994) · Archives of Microbiology · doi:10.1007/bf00264384 · Cited by 50
Lipid peroxidation in low density lipoproteins from human plasma and egg yolk promotes accumulation of 1‐acyl analogues of platelet‐activating factor‐like lipids
Tokumura, A., Toujima, M., Yoshioka, Y., et al. (1996) · Lipids · doi:10.1007/bf02587909 · Cited by 50
Disturbances in the composition of plasma lipoproteins during gram-negative sepsis in the rat
Lanza‐Jacoby, S., Wong, S. H., Tabares, A., Baer, D. J., & Schneider, T. G. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90134-h · Cited by 47
Adrenergic stimulation of phosphatidylinositol labelling in rat vas deferens
Scarnati, O. C. D., & Lapetina, E. G. (1974) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(74)90059-9 · Cited by 50
Oxidized lipoproteins found in patients with NIDDM stimulate radical-induced monocyte chemoattractant protein-1 mRNA expression in cultured human endothelial cells
Takahara, N., Kashiwagi, A., Nishio, Y., Harada, N., Kojima, H., Maegawa, H., Hidaka, H., & Kikkawa, R. (1997) · Diabetologia · doi:10.1007/s001250050731 · Cited by 50
Minimally oxidized LDL is a potent inhibitor of lecithin:cholesterol acyltransferase activity
Bielicki, J. K., Forte, T. M., & McCall, M. R. (1996) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42011-5 · Cited by 50
Reconstitution and Partial Characterization of Phospholipid Flippase Activity from Detergent Extracts of the Bacillus subtilis Cell Membrane
Hrafnsdóttir, S., & Menon, A. K. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.15.4198-4206.2000 · Cited by 50
Immunoglobulin E–dependent Active Fatal Anaphylaxis in Mast Cell–deficient Mice
Choi, I.-H., Shin, Y. M., Park, J. S., et al. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.9.1587 · Cited by 50
Acyl-coenzyme A: Cholesterol acyltransferase inhibitor, avasimibe, stimulates bile acid synthesis and cholesterol 7α-hydroxylase in cultured rat hepatocytes and in vivo in the rat
Post, S. M., Zoeteweij, P. J., Bos, M. H. A., de Wit, E. C., Havinga, R., Kuipers, F., & Princen, H. M. G. (1999) · Hepatology · doi:10.1002/hep.510300230 · Cited by 50
Farnesol inhibits phosphatidylcholine biosynthesis in cultured cells by decreasing cholinephosphotransferase activity
Voziyan, P., Goldner, C. M., & Melnykovych, G. (1993) · Biochemical Journal · doi:10.1042/bj2950757 · Cited by 50
Distinct sites of action of Bcl-2 and Bcl-xL in the ceramide pathway of apoptosis
El-Assaad, W., El-Sabban, M. E., Awaraji, C., et al. (1998) · Biochemical Journal · doi:10.1042/bj3360735 · Cited by 50
Isolation and properties of an extracellular protease of Trichophyton granulosum
Day, W. C., Toncic, P. F., Stratman, S. L., et al. (1968) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(68)90050-8 · Cited by 50
Platelet-activating factor (PAF) stimulates the PAF-synthesizing enzyme acetyl-CoA:1-alkyl-sn-glycero-3-phosphocholine O2-acetyltransferase and PAF synthesis in neutrophils.
Doebber, T. W., & Wu, M. S. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7557 · Cited by 50
Binding of fluorescent-labeled phosphatidylcholine to rat liver nonspecific lipid transfer protein.
Nichols, J. L. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47920-3 · Cited by 50
The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules
Stubbs, C. D., Kinosita, K., Munkonge, F. M., et al. (1984) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(84)90193-7 · Cited by 50
The Effect of Dietary Fat and Tocopherol on Lipolysis and Oxidation in Turkey Meat Stored at Different Temperatures
Sklan, D. J., Tenne, Z., & Budowski, P. (1983) · Poultry Science · doi:10.3382/ps.0622017 · Cited by 50
Chemical modification of acyl-CoA:cholesterol-O-acyltransferase. 1. Identification of acyl-CoA:cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity
Kinnunen, P. M., DeMichele, A., & Lange, L. G. (1988) · Biochemistry · doi:10.1021/bi00419a025 · Cited by 50
Utilization of exogenous fatty acids for complex lipid biosynthesis and its effect on de novo fatty acid formation in Escherichia coli K-12
Silbert, D. F., Ulbright, T. M., & Honegger, J. L. (1973) · Biochemistry · doi:10.1021/bi00725a027 · Cited by 50
Biochemical studies of the excitable membrane of Paramecium tetraurelia. II. Phospholipids of ciliary and other membranes
Andrews, D., & Nelson, D. L. (1979) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(79)90205-0 · Cited by 50
Solubilization, partial purification and characterization of rat liver microsomal diacylglycerol acyltransferase
Polokoff, M. A., & Bell, R. M. (1980) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(80)90060-0 · Cited by 49
The effect of fasting and refeeding on the composition and synthesis of triacylglycerols, phosphatidylcholines, and phosphatidylethanolamines in rat liver
Groener, J. E. M., Klein, W., & GOLDE, L. M. G. V. (1979) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(79)90421-1 · Cited by 50
Characterization of Pneumocystis carinii Preparations Developed for Lipid Analysis
Kaneshiro, E. S., Wyder, M. A., ZHOU, L. H., et al. (1993) · Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.1993.tb04479.x · Cited by 50
Purification of a 100 kDa phospholipase A2 from spleen, lung and kidney: antiserum raised to pig spleen phospholipase A2 recognizes a similar form in bovine lung, kidney and platelets, and immunoprecipitates phospholipase A2 activity
Kim, D. K., & Bonventre, J. V. (1993) · Biochemical Journal · doi:10.1042/bj2940261 · Cited by 50
Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in human hepatoma cell line Hep G2. Effects of inhibitors of cholesterol synthesis on enzyme activity
Boogaard, A. S., Griffioen, M., & Cohen, L. H. (1987) · Biochemical Journal · doi:10.1042/bj2410345 · Cited by 50
Phospholipid asymmetry in renal brush-border membranes
Vénien‐Bryan, C., & Grimellec, C. L. (1988) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(88)90285-4 · Cited by 50
Growth inhibitory protein(s) in the 3T3 cell plasma membrane. Partial purification and dissociation of growth inhibitory events from inhibition of amino acid transport
Raben, D., Lieberman, M. A., & Glaser, L. (1981) · Journal of Cellular Physiology · doi:10.1002/jcp.1041080106 · Cited by 50
Liquid Extraction: Bligh and Dyer
Sündermann, A., Eggers, L. F., & Schwudke, D. (2016) · Springer eBooks · doi:10.1007/978-94-007-7864-1_88-1 · Cited by 50
Species and Strain Selection
Borowitzka, M. A. (2013) · Algae for Biofuels and Energy · doi:10.1007/978-94-007-5479-9_4 · Cited by 50
Triacylglycerol and phytyl ester synthesis in Synechocystis sp. PCC6803
Aizouq, M., Peisker, H., Gutbrod, K., Melzer, M., Hölzl, G., & Dörmann, P. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1915930117 · Cited by 49
“The pellet was extracted three times with chloroform/methanol (1:2) (36).”
Method
Continuous catalytic deoxygenation of model and algal lipids to fuel-like hydrocarbons over Ni–Al layered double hydroxide
Santillan-Jimenez, E., Morgan, T., Loe, R., & Crocker, M. (2015) · Catalysis Today · doi:10.1016/j.cattod.2014.12.004 · Cited by 49
Quantification of phospholipid molecular species by coupled gas chromatography-mass spectrometry of deuterated samples
Dickens, B. F., Ramesha, C. S., & Thompson, G. A. (1982) · Analytical Biochemistry · doi:10.1016/0003-2697(82)90141-5 · Cited by 49
Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194
Gorte, O., Hollenbach, R., Papachristou, I., Steinweg, C., Silve, A., Frey, W., Syldatk, C., & Ochsenreither, K. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00355 · Cited by 48
“The supernatant was discarded and the biomass was preserved for 1 week at −20◦C for downstream processing using BM, HPH, or U combined with chloroform-methanol extraction according to Bligh and Dyer (BD) and Folch (F), or by using ethanol-hexane (EH) as extractants.”
BackgroundMethodInfluential
In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach
Tabernilla, A., Rodrigues, B. D. S., Pieters, A., Caufriez, A., Leroy, K., Campenhout, R. V., Cooreman, A., Gomes, A. R., Arnesdotter, E., Gijbels, E., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22095038 · Cited by 54
Seasonal and multi-annual variation in the abundance of isoprenoid GDGT membrane lipids and their producers in the water column of a meromictic equatorial crater lake (Lake Chala, East Africa)
Baxter, A. J., van Bree, L. G. J., Peterse, F., et al. (2021) · Quaternary Science Reviews · doi:10.1016/j.quascirev.2021.107263 · Cited by 49
Structure of a human intramembrane ceramidase explains enzymatic dysfunction found in leukodystrophy
Vasiliauskaité-Brooks, I., Healey, R. D., Rochaix, P., Saint‐Paul, J., Sounier, R., Grison, C. M., Waltrich-Augusto, T., Fortier, M., Hoh, F., Saied, E. M., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07864-w · Cited by 49
Targeting fungal membrane homeostasis with imidazopyrazoindoles impairs azole resistance and biofilm formation
Revie, N. M., Iyer, K. R., Maxson, M. E., Zhang, J., Yan, S., Fernandes, C. M., Meyer, K. J., Chen, X., Skulska, I., Fogal, M., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-31308-1 · Cited by 50
Recent Trends, Potentials, and Challenges of Biodiesel Production from Discarded Animal Fats: a Comprehensive Review
Binhweel, F., Hossain, M. S., & Ahmad, M. I. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10527-w · Cited by 51
Understanding the Performance of Plant Protein Concentrates as Partial Meat Substitutes in Hybrid Meat Emulsions
dos Santos, M., da Rocha, D. A. V. F., Bernardinelli, O. D., Júnior, F. D. O., de Sousa, D. G., Sabadini, E., Cunha, R. L., Trindade, M. A., & Pollonio, M. A. R. (2022) · Foods · doi:10.3390/foods11213311 · Cited by 56
Drought intensity alters productivity, carbon allocation and plant nitrogen uptake in fast versus slow grassland communities
Oram, N. J., Ingrisch, J., Bardgett, R. D., et al. (2023) · Journal of Ecology · doi:10.1111/1365-2745.14136 · Cited by 49
“…characterized the uptake of recent plant- derived C into broad microbial groups by determining 13C incorporation into PLFAs and NLFAs, which were extracted from frozen soil according to Bligh and Dyer (1959) and Karlowsky, Augusti, Ingrisch, Akanda, et al. (2018) (see Supplementary Methods).”
Method
Interaction between metformin and leucine in reducing hyperlipidemia and hepatic lipid accumulation in diet-induced obese mice
Fu, L., Bruckbauer, A., Li, F., Cao, Q., Cui, X., Wu, R., Shi, H., Zemel, M. B., & Xue, B. (2015) · Metabolism · doi:10.1016/j.metabol.2015.07.006 · Cited by 49
Conditions optimising on the yield of biomass, total lipid, and valuable fatty acids in two strains of Skeletonema menzelii
Jiang, X., Han, Q., Gao, X., & Gao, G. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.08.073 · Cited by 49
XBP-1-Deficient Plasmablasts Show Normal Protein Folding but Altered Glycosylation and Lipid Synthesis
McGehee, A. M., Dougan, S. K., Klemm, E. J., Shui, G., Park, B., Kim, Y., Watson, N., Wenk, M. R., Ploegh, H. L., & Hu, C. A. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0900953 · Cited by 49
The acyl dihydroxyacetone phosphate pathway enzymes for glycerolipid biosynthesis are present in the yeast Saccharomyces cerevisiae
Racenis, P. V., Lai, J., Das, A. K., Mullick, P. C., Hajra, A. K., & Greenberg, M. L. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.17.5702-5710.1992 · Cited by 49
Long-term effects of timber harvesting on hemicellulolytic microbial populations in coniferous forest soils
Leung, H., Maas, K. R., Wilhelm, R. C., & Mohn, W. W. (2015) · The ISME Journal · doi:10.1038/ismej.2015.118 · Cited by 54
Cold Exposure Partially Corrects Disturbances in Lipid Metabolism in a Male Mouse Model of Glucocorticoid Excess
van den Beukel, J. C., Boon, M. R., Steenbergen, J., Rensen, P. C. N., Meijer, O. C., Themmen, A. P. N., & Grefhorst, A. (2015) · Endocrinology · doi:10.1210/en.2015-1092 · Cited by 49
Lipid and sensory quality of canned Atlantic salmon (Salmo salar): Effect of the use of different seaweed extracts as covering liquids
Ortíz, J., Vivanco, J. P., & Aubourg, S. P. (2013) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201300239 · Cited by 49
Isolation and characterization of lipid rafts in E miliania huxleyi : a role for membrane microdomains in host–virus interactions
Rose, S. L., Fulton, J. M., Brown, C. M., Natale, F., Mooy, B. A. S. V., & Bidle, K. D. (2013) · Environmental Microbiology · doi:10.1111/1462-2920.12357 · Cited by 48
Cholesterol implications in plasmid DNA electrotransfer: Evidence for the involvement of endocytotic pathways
Rosazza, C., Phez, É., Escoffre, J., et al. (2011) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2011.05.024 · Cited by 49
Evaluation of Hg species after culinary treatments of fish
Schmidt, L. P. H., Bizzi, C. A., Duarte, F. A., et al. (2014) · Food Control · doi:10.1016/j.foodcont.2014.07.040 · Cited by 49
Lipid alterations in human blood-derived neutrophils lead to formation of neutrophil extracellular traps
Neumann, A., Brogden, G., Jerjomiceva, N., Brodesser, S., Naim, H. Y., & von Köckritz‐Blickwede, M. (2014) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2014.07.005 · Cited by 47
Flight metabolism in Panstrongylus megistus (Hemiptera: Reduviidae): the role of carbohydrates and lipids
Canavoso, L. E., Stariolo, R., & Rubiolo, E. R. (2003) · Memórias do Instituto Oswaldo Cruz · doi:10.1590/s0074-02762003000700009 · Cited by 49
Thiamine Prevents Obesity and Obesity-Associated Metabolic Disorders in OLETF Rats
Tanaka, T., Kono, T., Terasaki, F., YASUI, K., Soyama, A., Otsuka, K., FUJITA, S., YAMANE, K., MANABE, M., Kazuya, U. S. U. I., et al. (2010) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.56.335 · Cited by 50
Randomized Controlled Trial of Docosahexaenoic Acid Supplementation in Midwestern U.S. Human Milk Donors
Valentine, C. J., Morrow, G., Pennell, M. L., Morrow, A. L., Hodge, A., Haban-Bartz, A., Collins, K., & Rogers, L. K. (2012) · Breastfeeding Medicine · doi:10.1089/bfm.2011.0126 · Cited by 48
Spatial and seasonal differences in the PCB content of the mussel Mytilus edulis
Hummel, H., Bogaards, R. H., Nieuwenhuize, J., et al. (1990) · The Science of The Total Environment · doi:10.1016/0048-9697(90)90327-q · Cited by 49
High DHA content in Artemia is ineffective to improve Octopus vulgaris paralarvae rearing
Seixas, P., Rey‐Méndez, M., Valente, L. M. P., et al. (2009) · Aquaculture · doi:10.1016/j.aquaculture.2009.12.021 · Cited by 49
Microsomal Triglyceride Transfer Protein Enhances Cellular Cholesteryl Esterification by Relieving Product Inhibition
Iqbal, J., Rudel, L. L., & Hussain, M. M. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m800398200 · Cited by 49
Distinct Roles of the Phosphatidate Phosphatases Lipin 1 and 2 during Adipogenesis and Lipid Droplet Biogenesis in 3T3-L1 Cells
Sembongi, H., Miranda, M., Han, G., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.488445 · Cited by 49
Chronic Predation Risk Reduces Escape Speed by Increasing Oxidative Damage: A Deadly Cost of an Adaptive Antipredator Response
Janssens, L., & Stoks, R. (2014) · PLoS ONE · doi:10.1371/journal.pone.0101273 · Cited by 49
Sources of organic matter in sediments from the Ord River in tropical northern Australia
Volkman, J. K., Revill, A. T., Bonham, P. I., & Clementson, L. A. (2007) · Organic Geochemistry · doi:10.1016/j.orggeochem.2007.02.017 · Cited by 49
Delivery systems for natural antioxidant compounds: Archaeosomes and archaeosomal hydrogels characterization and release study
González-Paredes, A., Clares, B., Ruiz-Martínez, M. A., et al. (2011) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2011.09.042 · Cited by 49
Erythrocyte membrane omega-3 fatty acid levels and omega-3 fatty acid intake are not associated with conversion to type 1 diabetes in children with islet autoimmunity: The Diabetes Autoimmunity Study in the Young (DAISY)
Miller, M. R., Yin, X., Seifert, J. A., Clare-Salzler, M. J., Eisenbarth, G. S., Rewers, M., & Norris, J. M. (2011) · Pediatric Diabetes · doi:10.1111/j.1399-5448.2011.00760.x · Cited by 50
γ-Oryzanol Reduces Adhesion Molecule Expression in Vascular Endothelial Cells via Suppression of Nuclear Factor-κB Activation
Sakai, S., Murata, T., Tsubosaka, Y., et al. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf2043407 · Cited by 49
Nutritional Analysis of two Local Varieties of Papaya (Carica papaya L.) at Different Maturation Stages
Bari, L., Hassan, P., Absar, N., et al. (2005) · Pakistan Journal of Biological Sciences · doi:10.3923/pjbs.2006.137.140 · Cited by 49
Omega-3 Fatty Acids for Secondary Prevention of Posttraumatic Stress Disorder After Accidental Injury
Matsuoka, Y., Nishi, D., Yonemoto, N., Hamazaki, K., Hashimoto, K., & Hamazaki, T. (2010) · Journal of Clinical Psychopharmacology · doi:10.1097/jcp.0b013e3181d48830 · Cited by 49
The effect of copper ions on the lipid composition of subcellular membranes in Hydrilla verticillata
Розенцвет, О. А., Nesterov, V. N., & Sinyutina, N. F. (2012) · Chemosphere · doi:10.1016/j.chemosphere.2012.04.034 · Cited by 49
Neutral Sphingomyelinase 2 Deficiency Increases Hyaluronan Synthesis by Up-regulation of Hyaluronan Synthase 2 through Decreased Ceramide Production and Activation of Akt
Qin, J., Berdyshev, E. V., Poirer, C., Schwartz, N. B., & Dawson, G. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.304857 · Cited by 49
Amino acid substitution in NPC1 that abolishes cholesterol binding reproduces phenotype of complete NPC1 deficiency in mice
Xie, X., Brown, M. S., Shelton, J. M., Richardson, J. A., Goldstein, J. L., & Liang, G. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1112751108 · Cited by 50

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks