Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 12,801–12,900 of 48,846

Animal cell mutants defective in sterol metabolism: A specific selection procedure and partial characterization of defects
Saito, Y., Chou, S.-G., & Silbert, D. F. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.9.3730 · Cited by 51
The acylation of glycerol 3-phosphate in different rat organs and in the liver of different species (including man)
Daae, L. N. W. (1973) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(73)90224-5 · Cited by 51
Microalgae as future food: rich nutrients, safety, production costs and environmental effects
Gao, L., Qin, Y., Zhou, X., Jin, W., He, Z., Li, X., & Wang, Q. (2024) · Science of The Total Environment · doi:10.1016/j.scitotenv.2024.172167 · Cited by 55
Nitrogen, phosphorus and high CO2 modulate photosynthesis, biomass and lipid production in the green alga Chlorella vulgaris
Kumari, K., Samantaray, S., Sahoo, D., & Tripathy, B. C. (2021) · Photosynthesis Research · doi:10.1007/s11120-021-00828-0 · Cited by 50
“Extraction of lipid from the dried biomass was done following the protocol of binary solvents using chloroform and methanol according to Bligh and Dyer (1959).”
Method
Biodegradation of polyethylene by the marine fungus Parengyodontium album
Vaksmaa, A., Vielfaure, H., Polerecky, L., Kienhuis, M. V. M., van der Meer, M. T. J., Pflüger, T., Egger, M., & Niemann, H. (2024) · Science of The Total Environment · doi:10.1016/j.scitotenv.2024.172819 · Cited by 52
Effect of nitrogen source on growth and lipid accumulation in Scenedesmus abundans and Chlorella ellipsoidea
González-Garcinuño, Á., Tabernero, A., Sánchez-Álvarez, J. M., Martin del Valle, E. M., & Galán, M. A. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.09.038 · Cited by 50
Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis
Listenberger, L. L., Townsend, E., Rickertsen, C., Hains, A. E., Brown, E., Inwards, E. G., Stoeckman, A. K., Matis, M. P., Sampathkumar, R. S., Osna, N. A., et al. (2018) · Cells · doi:10.3390/cells7120230 · Cited by 49
Waste from brewing (trub) as a source of protein for the food industry
Saraiva, B. R., Anjo, F. A., Vital, A. C. P., et al. (2019) · International Journal of Food Science & Technology · doi:10.1111/ijfs.14101 · Cited by 50
The vitamin E derivative garcinoic acid from Garcinia kola nut seeds attenuates the inflammatory response
Wallert, M., Bauer, J., Kluge, S., Schmölz, L., Chen, Y., Ziegler, M., Searle, A. K., Maxones, A., Schubert, M., Thürmer, M., et al. (2019) · Redox Biology · doi:10.1016/j.redox.2019.101166 · Cited by 49
AIG1 and ADTRP are endogenous hydrolases of fatty acid esters of hydroxy fatty acids (FAHFAs) in mice
Ertunc, M. E., Kok, B. P., Parsons, W. H., Wang, J. G., Tan, D., Donaldson, C. J., Pinto, A. F. M., Vaughan, J. M., Ngo, N., Lum, K. M., et al. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.012145 · Cited by 50
“Lipids were extracted using a modified version of the BlighDyer method (49).”
Method
Comprehensive lipid analysis of green Arabica coffee beans by LC-HRMS/MS
Silva, A. C. R., da Silva, C. C., Garrett, R., & Rezende, C. M. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109727 · Cited by 49
Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli
Herndon, J., Peters, R. E., Hofer, R. N., Simmons, T. B., Symes, S. J. K., & Giles, D. K. (2020) · BMC Microbiology · doi:10.1186/s12866-020-01988-0 · Cited by 50
Redox imbalance impedes photosynthetic activity in rice by disrupting cellular membrane integrity and induces programmed cell death under submergence
Basu, S., Kumari, S., Kumar, P., Kumar, G., & Rajwanshi, R. (2021) · Physiologia Plantarum · doi:10.1111/ppl.13387 · Cited by 51
A novel microalgal biofilm reactor using walnut shell as substratum for microalgae biofilm cultivation and lipid accumulation
Zou, X., Xu, K., Chang, W., Qu, Y., & Li, Y. (2021) · Renewable Energy · doi:10.1016/j.renene.2021.04.122 · Cited by 48
Valorization of lipidic food waste for enhanced biodiesel recovery through two-step conversion: A novel microalgae-integrated approach
Almutairi, A. W., Al-Hasawi, Z. M., & Abomohra, A. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.125966 · Cited by 50
C24:0 Avoids Cold Exposure-Induced Oxidative Stress and Impaired Fatty Acid β -Oxidation
Sun, S., Cao, X., & Gao, J. (2021) · iScience · doi:10.1016/j.isci.2021.103409 · Cited by 53
“Total lipid was extracted by using the Bligh and Dyer procedure as previously described (Dyer and Bligh, 1959).”
Method
Plant community stability is associated with a decoupling of prokaryote and fungal soil networks
Zandt, D. I. ‘., Kolaříková, Z., Cajthaml, T., & Münzbergová, Z. (2023) · Nature Communications · doi:10.1038/s41467-023-39464-8 · Cited by 55
Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions
Xiao, Y., Zhang, J., Cui, J., Yao, X., Sun, Z., Feng, Y., & Cui, Q. (2015) · Algal Research · doi:10.1016/j.algal.2015.05.019 · Cited by 50
Green propolis modulates gut microbiota, reduces endotoxemia and expression of TLR4 pathway in mice fed a high-fat diet
Roquetto, A. R., Monteiro, N. E. S., Moura, C. S., Toreti, V. C., de Pace, F., Santos, A., Park, Y. K., & Amaya‐Farfán, J. (2015) · Food Research International · doi:10.1016/j.foodres.2015.07.026 · Cited by 52
Comparison of ambient solvent extraction methods for the analysis of fatty acids in non‐starch lipids of flour and starch
Bahrami, N., Yonekura, L., Linforth, R., et al. (2013) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.6449 · Cited by 50
Lipids and Composition of Fatty Acids of Saccharina latissima Cultivated Year-Round in Integrated Multi-Trophic Aquaculture
Marinho, G. S., Holdt, S. L., Jacobsen, C., & Angelidaki, İ. (2015) · Marine Drugs · doi:10.3390/md13074357 · Cited by 51
Effects of Gelatin-Based Edible Films Enriched with Laurel Essential Oil on the Quality of Rainbow Trout (Oncorhynchus mykiss) Fillets During Refrigerated Storage
Alparslan, Y., Baygar, T., Baygar, T., Yapıcı, H. H., & Metin, C. (2014) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 51
Chlamydia trachomatis Relies on Autonomous Phospholipid Synthesis for Membrane Biogenesis
Yao, J., Cherian, P., Frank, M. W., & Rock, C. O. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.657148 · Cited by 51
Chlamydia trachomatis Scavenges Host Fatty Acids for Phospholipid Synthesis via an Acyl-Acyl Carrier Protein Synthetase
Yao, J., Dodson, V. J., Frank, M. W., & Rock, C. O. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.671008 · Cited by 51
Isolation of lipids from biological samples
Furse, S., Egmond, M. R., & Killian, J. A. (2015) · Molecular Membrane Biology · doi:10.3109/09687688.2015.1050468 · Cited by 53
Botryococcus , what to do with it? Effect of nutrient concentration on biorefinery potential
Cabanelas, I. T. D., Marques, S. S. I., de Souza, C. O., Druzian, J. I., & Nascimento, I. A. (2015) · Algal Research · doi:10.1016/j.algal.2015.05.009 · Cited by 50
Iron in fortified biscuits: A simple method for its quantification, bioaccessibility study and physicochemical quality
Rebellato, A. P., Pacheco, B. C., Prado, J. P., et al. (2015) · Food Research International · doi:10.1016/j.foodres.2015.09.028 · Cited by 50
The Immunostimulatory Activity of Unmethylated and Methylated CpG Oligodeoxynucleotide Is Dependent on Their Ability To Colocalize with TLR9 in Late Endosomes
de Jong, S. D., Basha, G., Wilson, K. D., et al. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0802442 · Cited by 50
Identification and characterization of three genes encoding acyl-CoA: diacylglycerol acyltransferase (DGAT) from the microalga Myrmecia incisa Reisigl
Chen, C., Sun, Z., Cao, H., Fang, F.-L., Ouyang, L., & Zhou, Z. (2015) · Algal Research · doi:10.1016/j.algal.2015.09.007 · Cited by 49
Identification, Purification, and Characterization of Monoacylglycerol Acyltransferase from Developing Peanut Cotyledons
Tumaney, A. W., Shekar, S., & Rajasekharan, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100005200 · Cited by 50
Novel reactions in acyl editing of phosphatidylcholine by lysophosphatidylcholine transacylase (LPCT) and acyl-CoA:glycerophosphocholine acyltransferase (GPCAT) activities in microsomal preparations of plant tissues
Lager, I., Głąb, B., Eriksson, L., et al. (2014) · Planta · doi:10.1007/s00425-014-2184-1 · Cited by 50
Experimental evidence for the use of CCR2 antagonists in the treatment of type 2 diabetes
Sullivan, T. J., Miao, Z., Zhao, B. N., Ertl, L. S., Wang, Y., Krasinski, A., Walters, M. J., Powers, J. P., Dairaghi, D. J., Baumgart, T., et al. (2013) · Metabolism · doi:10.1016/j.metabol.2013.06.008 · Cited by 56
Inhibition of stearoyl-CoA desaturase-1 in differentiating 3T3-L1 preadipocytes upregulates elongase 6 and downregulates genes affecting triacylglycerol synthesis
Ralston, J. C., Badoud, F., Cattrysse, B., et al. (2014) · International Journal of Obesity · doi:10.1038/ijo.2014.35 · Cited by 50
Efficiencies of three common lipid extraction methods evaluated by calculating mass balances of the fatty acids
Xiao, L., Mjøs, S. A., & Haugsgjerd, B. O. (2011) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2011.08.003 · Cited by 50
Effect of water content and temperature on seed longevity of seven B rassicaceae species after 5 years of storage
Mira, S., Estrelles, E., & González-Benito, M. E. (2014) · Plant Biology · doi:10.1111/plb.12183 · Cited by 50
Antioxidative Activities of Mushroom ( Flammulina velutipes ) Extract Added to Bigeye Tuna Meat: Dose‐Dependent Efficacy and Comparison with Other Biological Antioxidants
Bao, H. N. D., Ushio, H., & Ohshima, T. (2009) · Journal of Food Science · doi:10.1111/j.1750-3841.2009.01069.x · Cited by 51
ω-3 oil intake during weight loss in obese women results in remodelling of plasma triglyceride and fatty acids
McCombie, G., Browning, L. M., Titman, C. M., Song, M., Shockcor, J. P., Jebb, S. A., & Griffin, J. L. (2009) · Metabolomics · doi:10.1007/s11306-009-0161-7 · Cited by 49
Single-cell oil production by cyanobacterium Aphanothece microscopica Nägeli cultivated heterotrophically in fish processing wastewater
Queiroz, M. I., Hornes, M. O., Manetti, A. G. D. S., & Lopes, E. J. (2011) · Applied Energy · doi:10.1016/j.apenergy.2010.12.047 · Cited by 49
Impact of Silver(I) on the Metabolism of Shewanella oneidensis
Wang, H., Law, N., Pearson, G., et al. (2009) · Journal of Bacteriology · doi:10.1128/jb.01277-09 · Cited by 50
Effect of lime application on microbial community in acidic tea orchard soils in comparison with those in wasteland and forest soils
Dong, X., Huang, X., Yao, H., et al. (2010) · Journal of Environmental Sciences · doi:10.1016/s1001-0742(09)60246-1 · Cited by 50
Dietary Omega-3 Polyunsaturated Fatty Acids Alter the Fatty Acid Composition of Hepatic and Plasma Bioactive Lipids in C57BL/6 Mice: A Lipidomic Approach
Balogun, K. A., Albert, C. J., Ford, D. A., Brown, R., & Cheema, S. K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0082399 · Cited by 51
Resistance to AFN-1252 Arises from Missense Mutations in Staphylococcus aureus Enoyl-acyl Carrier Protein Reductase (FabI)
Yao, J., Maxwell, J. B., & Rock, C. O. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.512905 · Cited by 51
A Novel Nontoxic Inhibitor of the Activation of NADPH Oxidase Reduces Reactive Oxygen Species Production in Mouse Lung
Lee, I., Dodia, C., Chatterjee, S., Zagorski, J., Mesaros, C., Blair, I. A., Feinstein, S. I., Jain, M. K., & Fisher, A. B. (2013) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.112.201079 · Cited by 53
Alkaline Ceramidase 2 (ACER2) and Its Product Dihydrosphingosine Mediate the Cytotoxicity of N-(4-Hydroxyphenyl)retinamide in Tumor Cells
Mao, Z., Sun, W., Xu, R., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.105296 · Cited by 50
Exposure of Enchytraeus albidus to Cd and Zn – Changes in cellular energy allocation (CEA) and linkage to transcriptional, enzymatic and reproductive effects
Novais, S. C., Soares, A. M. V. M., Coen, W. D., et al. (2012) · Chemosphere · doi:10.1016/j.chemosphere.2012.09.030 · Cited by 50
Involvement of Neutral Ceramidase in Ceramide Metabolism at the Plasma Membrane and in Extracellular Milieu
Tani, M., Igarashi, Y., & Ito, M. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m506827200 · Cited by 50
Loading of lipophorin particles with phospholipids at the midgut of Rhodnius prolixus
Atella, G. C., Gondim, K. C., & Masuda, H. (1995) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.940300404 · Cited by 50
Quantification of Phosphatidic Acid in Foodstuffs Using a Thin-Layer-Chromatography-Imaging Technique
Tanaka, T., Kassai, A., Ohmoto, M., Morito, K., Kashiwada, Y., Takaishi, Y., Urikura, M., Morishige, J., Satouchi, K., & Tokumura, A. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf300147y · Cited by 50
Importance of Specific Native Lipids in Controlling the Photocycle of Bacteriorhodopsin
Joshi, M. K., Dracheva, S., Mukhopadhyay, A. K., et al. (1998) · Biochemistry · doi:10.1021/bi980965j · Cited by 50
Action of Shiga Toxin Type-2 and Subtilase Cytotoxin on Human Microvascular Endothelial Cells
Amaral, M. M., Sacerdoti, F., Jancic, C. C., Repetto, H. A., Paton, A. W., Paton, J. C., & Ibarra, C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0070431 · Cited by 53
Rapid and simple extraction of lipids from blood plasma and urine for liquid chromatography-tandem mass spectrometry
Bang, D. Y., Byeon, S. K., & Moon, M. H. (2014) · Journal of Chromatography A · doi:10.1016/j.chroma.2014.01.024 · Cited by 51
Inhibition of leukotriene synthesis, pharmacokinetics, and tolerability of a novel dietary fatty acid formulation in healthy adult subjects
Surette, M. E., Koumenis, I. L., Edens, M. B., et al. (2003) · Clinical Therapeutics · doi:10.1016/s0149-2918(03)80116-9 · Cited by 50
The use of a soy product in juvenile yellowtail kingfish (Seriola lalandi) feeds at different water temperatures: 2. Soy protein concentrate
Bowyer, J. N., Qin, J. G., Smullen, R. P., Adams, L. R., Thomson, M. J. S., & Stone, D. A. J. (2013) · Aquaculture · doi:10.1016/j.aquaculture.2013.06.001 · Cited by 50
Criteria for assessing larval and postlarval quality of Pacific white shrimp (Litopenaeus vannamei, Boone, 1931)
Racotta, I. S. (2003) · Aquaculture · doi:10.1016/j.aquaculture.2003.09.031 · Cited by 50
Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription
Viegas, C. A., Cabral, M. G., Teixeira, M. C., Neumann, G., Heipieper, H. J., & Sá‐Correia, I. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.02.158 · Cited by 49
Identification of Mg2+-dependent Neutral Sphingomyelinase 1 as a Mediator of Heat Stress-induced Ceramide Generation and Apoptosis
Yabu, T., Imamura, S., Yamashita, M., et al. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m805402200 · Cited by 50
Developmental Programming: Exposure to Testosterone Excess Disrupts Steroidal and Metabolic Environment in Pregnant Sheep
Salloum, B. A., Veiga‐Lopez, A., Abbott, D. H., Burant, C., & Padmanabhan, V. (2015) · Endocrinology · doi:10.1210/en.2014-2006 · Cited by 51
Production of lipids and formation and mobilization of lipid bodies in Chlorella vulgaris
Přibyl, P., Cepák, V., & Zachleder, V. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-012-9889-y · Cited by 48
Compositional properties and bioactive potential of waste material from shrimp cooking juice
Pérez‐Santín, E., Calvo, M. M., López-Caballero, M. E., Montero, P., & Gómez-Guillén, M. C. (2013) · LWT · doi:10.1016/j.lwt.2013.05.038 · Cited by 49
Microbial lipid production: screening with yeasts grown on Brazilian molasses
Vieira, J. P. F., Ienczak, J. L., Rossell, C. E. V., et al. (2014) · Biotechnology Letters · doi:10.1007/s10529-014-1624-0 · Cited by 50
Effect of salting processes on chemical composition, textural properties and microstructure of duck egg
Kaewmanee, T., Benjakul, S., & Visessanguan, W. (2009) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.3492 · Cited by 50
Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice
Park, H., Ha, S. K., Eom, H., & Choi, I. (2013) · Food and Chemical Toxicology · doi:10.1016/j.fct.2013.01.060 · Cited by 50
Endosymbiotic heterocystous cyanobacteria synthesize different heterocyst glycolipids than free-living heterocystous cyanobacteria
Schouten, S., Villareal, T. A., Hopmans, E. C., et al. (2012) · Phytochemistry · doi:10.1016/j.phytochem.2012.09.002 · Cited by 50
Soil microbial community response and recovery following group selection harvest: Temporal patterns from an experimental harvest in a US northern hardwood forest
Lewandowski, T. E., Forrester, J. A., Mladenoff, D. J., Stoffel, J. L., Gower, S. T., D’Amato, A. W., & Balser, T. C. (2015) · Forest Ecology and Management · doi:10.1016/j.foreco.2014.12.012 · Cited by 50
Anti-hyperglycemic effects of three medicinal plants in diabetic pregnancy: modulation of T cell proliferation
Yessoufou, A., Gbénou, J., Grissa, O., Hichami, A., Simonin, A.-M., Tabka, Z., Moudachirou, M., Moutairou, K. A., & Khan, N. A. (2013) · BMC Complementary and Alternative Medicine · doi:10.1186/1472-6882-13-77 · Cited by 50
Sequential Ordered Fatty Acid α Oxidation and Δ9 Desaturation Are Major Determinants of Lipid Storage and Utilization in Differentiating Adipocytes
Su, X., Han, X., Yang, J., Mancuso, D. J., Chen, J., Bickel, P. E., & Gross, R. W. (2004) · Biochemistry · doi:10.1021/bi035867z · Cited by 50
Successional changes in the soil microbial community along a vegetation development sequence in a subalpine volcanic desert on Mount Fuji, Japan
Yoshitake, S., Fujiyoshi, M., Watanabe, K., et al. (2012) · Plant and Soil · doi:10.1007/s11104-012-1348-7 · Cited by 50
Differential Mobilization of Blubber Fatty Acids in Lactating Weddell Seals: Evidence for Selective Use
Wheatley, K. E., Nichols, P. D., Hindell, M. A., et al. (2008) · Physiological and Biochemical Zoology · doi:10.1086/590397 · Cited by 50
Analytical approaches to determination of total choline in foods and dietary supplements
Phillips, M. M. (2012) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-5652-5 · Cited by 50
Defining fish community structure in Lake Winnipeg using stable isotopes (δ13C, δ15N, δ34S): Implications for monitoring ecological responses and trophodynamics of mercury & other trace elements
Ofukany, A. F. A., Wassenaar, L. I., Bond, A. L., & Hobson, K. A. (2014) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2014.07.125 · Cited by 50
The role of cholesterol-sphingomyelin membrane nanodomains in the stability of intercellular membrane nanotubes
Kabaso, D. (2012) · International Journal of Nanomedicine · doi:10.2147/ijn.s28723 · Cited by 50
Sorption and degradation of triclosan in sediments and its effect on microbes
Huang, X., Wu, C., Hu, H., Yu, Y., & Liu, J. (2015) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2015.03.002 · Cited by 48
CGI-58/ABHD5-Derived Signaling Lipids Regulate Systemic Inflammation and Insulin Action
Lord, C. C., Betters, J. L., Ivanova, P. T., Milne, S. B., Myers, D. S., Madenspacher, J., Thomas, G., Chung, S., Liu, M., Davis, M. A., et al. (2012) · Diabetes · doi:10.2337/db11-0994 · Cited by 45
Fussy Feeders: Phyllosoma Larvae of the Western Rocklobster (Panulirus cygnus) Demonstrate Prey Preference
Saunders, M. I., THOMPSON, P. A., JEFFS, A. G., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0036580 · Cited by 50
Salinity induces membrane structure and lipid changes in maize mesophyll and bundle sheath chloroplasts
Omoto, E., Iwasaki, Y., Miyake, H., et al. (2015) · Physiologia Plantarum · doi:10.1111/ppl.12404 · Cited by 50
Chronic administration of recombinant IL-6 upregulates lipogenic enzyme expression and aggravates high fat diet-induced steatosis in IL-6 deficient mice
Vida, M., Gavito, A., Pavón, F. J., Bautista, D., Serrano, A., Suárez, J., Arrabal, S., Decara, J. M., Cuevas, M. R., de Fonseca, F. R., et al. (2015) · Disease Models & Mechanisms · doi:10.1242/dmm.019166 · Cited by 53
Influence of gap size on carbon and nitrogen biogeochemical cycling in Northern hardwood forests of the Upper Peninsula, Michigan
Schliemann, S. A., & Bockheim, J. G. (2014) · Plant and Soil · doi:10.1007/s11104-013-2005-5 · Cited by 50
Geranylgeranyl Reductase and Ferredoxin from Methanosarcina acetivorans Are Required for the Synthesis of Fully Reduced Archaeal Membrane Lipid in Escherichia coli Cells
Isobe, K., Ogawa, T., Hirose, K., Yokoi, T., Yoshimura, T., & Hemmi, H. (2013) · Journal of Bacteriology · doi:10.1128/jb.00927-13 · Cited by 50
Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis
Schmidt, H., Hansen, G., Singh, S. K., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1119894109 · Cited by 50
Replacement of fish meal with a matrix of organic plant proteins in organic trout (Oncorhynchus mykiss) feed, and the effects on nutrient utilization and fish performance
Lund, I., Dalsgaard, J., Rasmussen, H. T., et al. (2011) · Aquaculture · doi:10.1016/j.aquaculture.2011.09.028 · Cited by 50
Comparison of lipids, fatty acids and volatile compounds of various kumquat species using HS / GC / MS / FID techniques
Güney, M., Öz, A. T., & Kafkas, E. (2014) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.6817 · Cited by 50
Enhanced palm oil mill effluent treatment and biomethane production by co‐digestion of oil palm empty fruit bunches with Chlorella Sp
Ahmad, A., Shah, S. M. U., Othman, M. F., & Abdullah, M. A. (2014) · The Canadian Journal of Chemical Engineering · doi:10.1002/cjce.22029 · Cited by 50
Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate-capture molecule
Tanaka, T., Tsutsui, H., Hirano, K., et al. (2004) · Journal of Lipid Research · doi:10.1194/jlr.d400010-jlr200 · Cited by 50
Effects of rosemary and green tea extracts on frozen surimi gels fortified with omega‐3 fatty acids
Pérez-Mateos, M., Lanier, T. C., & Boyd, L. C. (2005) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2388 · Cited by 50
Intracellular Phospholipase A1and Acyltransferase, Which Are Involved inCaenorhabditis elegansStem Cell Divisions, Determine thesn-1 Fatty Acyl Chain of Phosphatidylinositol
Imae, R., Inoué, T., Kimura, M., Kanamori, T., Tomioka, N. H., Kage‐Nakadai, E., Mitani, S., & Arai, H. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-03-0195 · Cited by 55
Choline deficiency impairs intestinal lipid metabolism in the lactating rat
da Silva, R. P., Kelly, K. B., Lewis, E. D., et al. (2015) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2015.04.015 · Cited by 50
Myeloperoxidase formation of PAF receptor ligands induces PAF receptor-dependent kidney injury during ethanol consumption
Latchoumycandane, C., Nagy, L. E., & McIntyre, T. M. (2015) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2015.05.020 · Cited by 50
Forage fish quality: seasonal lipid dynamics of herring (Clupea harengus L.) and sprat (Sprattus sprattus L.) in the Baltic Sea
Røjbek, M., Tomkiewicz, J., Jacobsen, C., et al. (2013) · ICES Journal of Marine Science · doi:10.1093/icesjms/fst106 · Cited by 50
A STUDY ON THE FEEDING ECOLOGY OF CHITONS USING ANALYSIS OF GUT CONTENTS AND FATTY ACID MARKERS
Латышев, Н. А. (2004) · Journal of Molluscan Studies · doi:10.1093/mollus/70.3.225 · Cited by 50
TNFα gene knockout differentially affects lipid deposition in liver and skeletal muscle of high-fat-diet mice
Salles, J., Tardif, N., Landrier, J., Mothe‐Satney, I., Guillet, C., Vaysse, C., Combaret, L., Giraudet, C., Patrac, V., Bertrand‐Michel, J., et al. (2012) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2011.12.001 · Cited by 52
Maradolipids: Diacyltrehalose Glycolipids Specific to Dauer Larva in Caenorhabditis elegans
Penkov, S., Mende, F., Zagoriy, V., et al. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.201004466 · Cited by 50
Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival
Dória, M. L., Ribeiro, A. S., Wang, J., Cotrim, C. Z., Domingues, P. M., Williams, C., Domingues, M. R., & Helguero, L. A. (2014) · The FASEB Journal · doi:10.1096/fj.14-249672 · Cited by 50
Discovering and validating between-subject variations in plasma lipids in healthy subjects
Begum, H., Li, B., Shui, G., Cazenave‐Gassiot, A., Soong, R. C. T., Ong, R. T., Little, P., Teo, Y., & Wenk, M. R. (2016) · Scientific Reports · doi:10.1038/srep19139 · Cited by 50
Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid
Kitatani, K., Usui, T., Sriraman, S. K., Toyoshima, M., Ishibashi, M., Shigeta, S., Nagase, S., Sakamoto, M., Ogiso, H., Okazaki, T., et al. (2015) · Oncogene · doi:10.1038/onc.2015.330 · Cited by 51
Animal Models in Eye Research
Robinson, M. L. (2009) · Human Genomics · doi:10.1186/1479-7364-3-4-381 · Cited by 50
Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions
da Silva, H. R., Prete, C. E. C., Gavilanes, F. Z., et al. (2016) · AMB Express · doi:10.1186/s13568-016-0180-5 · Cited by 50
Dietary lipid concentrations influence growth, liver oxidative stress, and serum metabolites of juvenile hybrid snakehead (Channa argus × Channa maculata)
Zhao, P., Li, F., Chen, X., et al. (2016) · Aquaculture International · doi:10.1007/s10499-016-9993-0 · Cited by 50
Metabolic engineering of Pichia pastoris to produce ricinoleic acid, a hydroxy fatty acid of industrial importance
Meesapyodsuk, D., Chen, Y., Ng, S. H., et al. (2015) · Journal of Lipid Research · doi:10.1194/jlr.m060954 · Cited by 50
Kinetics of Peptides and Arginine Production from Microalgae ( Scenedesmus sp .) by Flash Hydrolysis
Garcia-Moscoso, J. L., Teymouri, A., & Kumar, S. (2015) · Industrial & Engineering Chemistry Research · doi:10.1021/ie5047279 · Cited by 51
Comprehensive and Quantitative Analysis of Lysophospholipid Molecular Species Present in Obese Mouse Liver by Shotgun Lipidomics
Wang, C., Wang, M., & Han, X. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b00410 · Cited by 50

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks