Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Li, S., Wang, T., Fu, W., Kennett, M. J., Cox, A. D., Lee, D. Y., Vanamala, J. K. P., & Reddivari, L. (2021) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202100152 · Cited by 24
Fatty Acid Profiles of Selected Microalgae Used as Live Feeds for Shrimp Postlarvae in Vietnam
Duc, T., Chang, K. J. L., Jameson, I. D., Hoang, T., Cai, N. B. A., & Pham, H. Q. (2021) · Aquaculture Journal · doi:10.3390/aquacj1010004 · Cited by 23
“Samples (10 mg) were extracted quantitatively by the modified dichloromethane (DCM)-methanol (MeOH)–water Bligh and Dyer (1959) method [24].”
Method
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Zhang, D., Li, Q., Yan, C., & Cong, W. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02607-9 · Cited by 23
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AbdElgawad, H., Okla, M. K., Al-amri, S. S., et al. (2021) · Plants · doi:10.3390/plants10112434 · Cited by 23
Biodiesel potentials of microalgal strains isolated from fresh water environment
Azeez, N. A., Oyelami, S., Adekanmi, A. A., Ologunye, O. B., Adedigba, S. A., Akinola, O. J., & ADEDUNTAN, A. S. (2021) · Environmental Challenges · doi:10.1016/j.envc.2021.100367 · Cited by 24
Can Fish and Shellfish Species from the Black Sea Supply Health Beneficial Amounts of Bioactive Fatty Acids?
Merdzhanova, A., Panayotova, V., Dobreva, D. A., & Peycheva, K. (2021) · Biomolecules · doi:10.3390/biom11111661 · Cited by 25
“Total lipids (TL) were extracted according to the method of Bligh and Dyer [26].”
Method
High intensity of light: A potential stimulus for maximizing biomass by inducing photosynthetic activity in marine microalga, Tetraselmis tetrathele
Farahin, A. W., Ikhsan, N. F. M., Nagao, N., Katayama, T., Imaizumi, Y., Mamat, N. Z., Yusoff, F. M., & Shariff, M. (2021) · Algal Research · doi:10.1016/j.algal.2021.102523 · Cited by 25
Instantaneous characterization of crude vegetable oils via triacylglycerols fingerprint by atmospheric solids analysis probe tandem mass spectrometry with multiple neutral loss scans
Pizzo, J. S., da Cruz, V. H. M., Santos, P. D. S., et al. (2021) · Food Control · doi:10.1016/j.foodcont.2021.108710 · Cited by 23
Drug Repositioning for Fabry Disease: Acetylsalicylic Acid Potentiates the Stabilization of Lysosomal Alpha-Galactosidase by Pharmacological Chaperones
Monticelli, M., Liguori, L., Allocca, M., Bosso, A., Andreotti, G., Lukáš, J., Monti, M. C., Morretta, E., Cubellis, M. V., & Mele, B. H. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23095105 · Cited by 25
“The extraction was accomplished according to the protocol outlined by Bligh and Dyer [88] with a few modifications.”
Method
De novo synthesized polyunsaturated fatty acids operate as both host immunomodulators and nutrients for Mycobacterium tuberculosis
Laval, T., Pedró-Cos, L., Malaga, W., Guenin‐Macé, L., Pawlik, A., Mayau, V., Yahia‐Cherbal, H., Delos, O., Frigui, W., Bertrand‐Michel, J., et al. (2021) · eLife · doi:10.7554/elife.71946 · Cited by 27
Low levels of linoleic acid and α-linolenic acid and high levels of arachidonic acid in plasma phospholipids are associated with hypertension
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Nguyen, H. T., Bao, H. N. D., Dang, H. T. T., et al. (2022) · Foods · doi:10.3390/foods11111531 · Cited by 23
“Lipids were measured according to the Bligh and Dyer [43] method.”
Method
Identification of icaritin derivative IC2 as an SCD-1 inhibitor with anti-breast cancer properties through induction of cell apoptosis
Yang, C., Jin, Y.-Y., Mei, J., et al. (2022) · Cancer Cell International · doi:10.1186/s12935-022-02621-y · Cited by 23
Effect of surfactants on the production and biofunction of Tremella fuciformis polysaccharide through submerged fermentation
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Improvement of phosphate solubilizing bacteria Paenibacillus xylanexedens on the growth of Chlorella pyrenoidosa and wastewater treatment in attached cultivation
Dong, H., Liu, W., Zhang, H., Zheng, X., Duan, H., Zhou, L., Xu, T., & Ruan, R. R. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.135604 · Cited by 23
Insulin-like growth factor 1 receptor mediates photoreceptor neuroprotection
Rajala, A., Teel, K., Bhat, M. A., Batushansky, A., Griffin, T. M., Purcell, L., & Rajala, R. V. S. (2022) · Cell Death and Disease · doi:10.1038/s41419-022-05074-3 · Cited by 22
“Total lipids were extracted following the method of Bligh and Dyer [50], with modifications [51].”
Method
Exploratory lipidome and metabolome profiling contributes to understanding differences in high and normal ultimate pH beef
Antonelo, D. S., dos Santos-Donado, P. R., Ferreira, C. R., et al. (2022) · Meat Science · doi:10.1016/j.meatsci.2022.108978 · Cited by 23
Enhanced productivity of extracellular free fatty acids by gene disruptions of acyl-ACP synthetase and S-layer protein in Synechocystis sp. PCC 6803
Eungrasamee, K., Lindblad, P. C., & Jantaro, S. (2022) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-022-02197-9 · Cited by 26
“The lipids were extracted according to the Bligh and Dyer method [47] with slight modification.”
Method
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Kaur, M., Li, J., Zhang, P., Yang, H., Wang, L., & Xu, M. (2022) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2022.1026771 · Cited by 26
Bioconversion of sugarcane molasses and waste glycerol on single cell oils for biodiesel by the red yeast Rhodotorula glutinis R4 from Antarctica
Sineli, P. E., Maza, D. D., Aybar, M. J., de Figueroa, L. I. C., & Viñarta, S. C. (2022) · Energy Conversion and Management X · doi:10.1016/j.ecmx.2022.100331 · Cited by 23
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Hydrodynamic and geochemical controls on soil carbon mineralization upon entry into aquatic systems
Liu, T., Liu, X., Pan, Q., Liu, S., & Feng, X. (2022) · Water Research · doi:10.1016/j.watres.2022.119499 · Cited by 24
Cardiac Transcriptome Remodeling and Impaired Bioenergetics in Single-Ventricle Congenital Heart Disease
Garcia, A. M., Toni, L. S., Miyano, C. A., Sparagna, G. C., Jonscher, R. L., Phillips, E. K., Karimpour‐Fard, A., Chapman, H. L., Baybayon-Grandgeorge, A. N., Pietra, A. E., et al. (2023) · JACC Basic to Translational Science · doi:10.1016/j.jacbts.2022.09.013 · Cited by 26
Distinctly localized lipid phosphate phosphatases mediate endoplasmic reticulum glycerolipid metabolism in Arabidopsis
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Nutritional composition, heavy metal contents and lipid quality of five marine fish species from Cameroon coast
Manz, J. C. K., Nsoga, J. V. F., Diazenza, J. B., et al. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e14031 · Cited by 23
“Lipid content Total lipid was determined by Bligh and Dyer [21] method using chloroform/methanol/water 2:2:1.”
MethodInfluential
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Carr, E., Barton, Q., Grambo, S., Sullivan, M., Renfro, C. M., Kuo, A., Pangilinan, J. L., Lipzen, A., Keymanesh, K., Savage, E., et al. (2023) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkad110 · Cited by 23
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Ying, Z., van Eenige, R., Ge, X., van Marwijk, C., Lambooij, J. M., Guigas, B., Giera, M. A., de Boer, J. F., Coşkun, T., Qu, H., et al. (2023) · EBioMedicine · doi:10.1016/j.ebiom.2023.104684 · Cited by 26
Characterization of lipids in insect powders
Brogan, E. N., Park, Y., Shen, C., et al. (2023) · LWT · doi:10.1016/j.lwt.2023.115040 · Cited by 23
Effects of winter grazing and N addition on soil phosphorus fractions in an alpine grassland on the Qinghai-Tibet Plateau
Guan, Z., Cao, Z., Li, X. G., et al. (2023) · Agriculture Ecosystems & Environment · doi:10.1016/j.agee.2023.108700 · Cited by 23

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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