Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“The composition was determined based on analyses of dry matter, ash, crude protein, total dietary fiber (AOAC 1995) and fat (Bligh and Dyer 1959).”
MethodInfluential
Constructed Wetland and Microalgae: A Revolutionary Approach of Bioremediation and Sustainable Energy Production
Upadhyay, A. K., & Mandotra, S. K. (2020) · Springer eBooks · doi:10.1007/978-981-15-7518-1_2 · Cited by 2
Fatty acid profiles of waste fish skins and potential use for crackers
Yavuzer, E. (2020) · Journal of Anatolian Environmental and Animal Sciences · doi:10.35229/jaes.766584 · Cited by 2
Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes
Hasni, I., Armstrong, N., Decloquement, P., et al. (2020) · Microorganisms · doi:10.3390/microorganisms8111791 · Cited by 2
“Total lipids were extracted according to the Bligh and Dyer protocol [56].”
Method
Corrosion behavior of carbon steel in the presence of iron-oxidizing bacterium Pseudomonas sp. DASEWM1
Sachan, R., & Singh, A. (2020) · Environmental Sustainability · doi:10.1007/s42398-020-00144-8 · Cited by 2
The effect of different application methods of sumac ( Rhus coriaria ) and tarragon ( Artemisia dracunculus ) on nutritional composition, fatty acids and TBARS values of marinated sea bream ( Sparus aurata L., 1758)
Çorapçı, B., Köstekli̇, B., EYUBOĞLU, A., & Kocatepe, D. (2020) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.15102 · Cited by 2
Uskumru, Palamut ve Levrek Balıklarının Fırında Pişirme İşlemi Sonrasında Kalan Balık Sularının Yağ Asidi İçeriğinin Belirlenmesi ve Balık Suyu Çorbası Yapımında Kullanımı
Yavuzer, E. (2020) · Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi · doi:10.25308/aduziraat.757938 · Cited by 2
GLUTENSİZ NUGGETLARDA LİPOLİTİK DEĞİŞİMLER ÜZERİNE DONMUŞ DEPOLAMANIN ETKİSİ
Davarcıoğlu, E. S., & Soncu, E. D. (2020) · GIDA / THE JOURNAL OF FOOD · doi:10.15237/gida.gd20100 · Cited by 2
Double‑lobectomy in a steatotic liver transplantation rat model
Fan, L., Fu, Z., Xiong, Y., et al. (2021) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2021.9687 · Cited by 2
“Lipids from rat livers were prepared using chloroform‐methanol extrac‐ tion (30).”
The role of increased FGF21 in VLDL-TAG secretion and thermogenic gene expression in mice under protein malnutrition
Ozaki‐Masuzawa, Y., Kosaka, H., Abiru, R., Toda, Y., Kawabata, K., Nagata, M., Hara, S., Konishi, M., Itoh, N., Hosono, T., et al. (2021) · Bioscience Biotechnology and Biochemistry · doi:10.1093/bbb/zbab030 · Cited by 2
Effects of Dietary Supplementation with Myo-inositol on Hepatic Expression of Glycolytic and Fructolytic Enzyme Genes in Rats Fed a High-sucrose Diet
Hibi, M., Sugiura, S., Nakagawa, T., Hayakawa, T., & Shimada, M. (2021) · Journal of Oleo Science · doi:10.5650/jos.ess20340 · Cited by 2
Microalgae preparation and lipid extraction by subcritical dimethyl ether
Wang, Q., Oshita, K., Takaoka, M., et al. (2021) · MethodsX · doi:10.1016/j.mex.2021.101353 · Cited by 2
“The B&D method [4] was usually used as a standard for the total lipid content of microalgae.”
Method
Antioxidant Properties of Metabolites from New Extremophiles Microalgal Strain (Southern, Tunisia)
Gammoudi, S., Moussa, I. D., Annabi-Trabelsi, N., Ayadi, H., & Guermazi, W. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.96777 · Cited by 3
Improvements in the cultivation of Botryococcus braunii using commercial fertilisers
Bermejo, E., González, C., Montero, Z., Vı́lchez, C., Garbayo, I., & Cuaresma, M. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02486-0 · Cited by 2
“(1957) and Bligh and Dyer (1959) .”
Köyceğiz Lagünü’nden Avlanan Altınbaş (Chelon auratus) ve Mavri (Chelon labrosus) Kefallerinin Besin Kompozisyonu ve Yağ Asidi Profilinin Mevsimsel Değişimi
Metin, C., Alparslan, Y., & Baygar, T. (2021) · Acta Aquatica Turcica · doi:10.22392/actaquatr.773796 · Cited by 2
Effect of Processing on Physicochemical Properties and Fatty Acid Composition of Fluted Pumpkin (Telfairia occidentalis) Seed Oil
Alademeyin, J. O., & Arawande, J. O. (2016) · Pakistan Journal of Scientific & Industrial Research Series A Physical Sciences · doi:10.52763/pjsir.phys.sci.59.2.2016.83.89 · Cited by 2
Long-chain n-3 polyunsaturated fatty acids contained in liver and muscle tissues of Okinawan long-spine porcupinefish (Diodon holocanthus Linnaeus 1758)
Tashiro, Y. (2021) · Nutrition & Food Science · doi:10.1108/nfs-03-2021-0089 · Cited by 2
Methyloradius palustris gen. nov., sp. nov., a methanol-oxidizing bacterium isolated from snow
Miyadera, T., Kojima, H., & Fukui, M. (2021) · Archives of Microbiology · doi:10.1007/s00203-021-02559-1 · Cited by 2
Pterygoplichthys spp. (Siluriformes: Loricariidae) meal is suitable for the culture of Nile tilapia Oreochromis niloticus (Cichlidae) juveniles
Cano-Salgado, M. P., Álvarez‐González, C. A., Martínez‐García, R., et al. (2021) · Ciência Rural · doi:10.1590/0103-8478cr20200801 · Cited by 2
Using segmental bioimpedance analysis to estimate soft tissue and chemical composition of retail cuts and carcasses of lambs
Moro, A. B., Montanholi, Y. R., Galvani, D. B., et al. (2021) · Meat Science · doi:10.1016/j.meatsci.2021.108644 · Cited by 2
Inhibition of platelet-activating factor (PAF)-induced platelet aggregation by fatty acids from human saliva
Smal, M. A., & Baldo, B. A. (2021) · Platelets · doi:10.1080/09537104.2021.1961705 · Cited by 2
“Pooled mixed human saliva (27 ml) was extracted with chloroform/methanol/water as described by Bligh and Dyer [15].”
Method
Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert
Xue, Z., Zhao, S., Bold, N., et al. (2021) · Molecules · doi:10.3390/molecules26185521 · Cited by 2
“Cells were collected by centrifuge at 12,000 rpm at 4 ◦C, and the cell mass was dried using a vacuum freeze-drying apparatus [39].”
Method
Variables that intervene in the weight of rotifer biomass and fatty acids
Samanamud, C. P. A., Gaspar, W., Inga, G., et al. (2021) · Journal of the World Aquaculture Society · doi:10.1111/jwas.12848 · Cited by 2

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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