Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 43,101–43,200 of 48,847

Beverage of Brazil Nut and Bocaiuva Almond Enriched with Minerals: Technological Quality and Nutritional Effect in Male Wistar Rats
de Oliveira, B. R., Egea, M. B., de França, S. A., Hernandes, T., & Takeuchi, K. P. (2024) · Foods · doi:10.3390/foods13162533 · Cited by 3
“The composition of the beverages was estimated using established methods [18] as follows: moisture, lipid [19], protein (conversion factor of 5.46 to Brazil nut and 5.18 to bocaiuva almond, No. 991.20), ash, and carbohydrate (3,5-dinitrosalicylic acid method including the sample hydrolysis step…”
MethodInfluential
Unconventional food plants in mixed juices: evaluation of the nutritional, physical-chemical, microbiological, and sensory quality of orange juice with sorrel ( Rumex acetosa ) and taioba ( Xanthosoma sagittifolium )
Mendes, F. D., Uliana, D. S., Silva, A. I. D. S., et al. (2024) · CyTA - Journal of Food · doi:10.1080/19476337.2024.2387716 · Cited by 2
“The determination of lipids was performed by the method of Bligh & Dyer (1959), with minor modifications.”
Fueling the Future: Extraction and Production Technological Development of Marine Macroalgae-Seaweed Biodiesel
Ahmad, M. I., Alsaadi, S., Shakir, M. A., et al. (2024) · Springer eBooks · doi:10.1007/978-981-97-5057-3_13 · Cited by 2
Sulfotransferase 1C2 Increases Mitochondrial Respiration by Converting Mitochondrial Membrane Cholesterol to Cholesterol Sulfate
Kolb, A. L., Corridon, P. R., Ullah, M. M., Pfaffenberger, Z. J., Xu, W. M., Winfree, S., Sandoval, R. M., Hato, T., Witzmann, F. A., Mohallem, R., et al. (2024) · Biochemistry · doi:10.1021/acs.biochem.3c00344 · Cited by 2
Effects of using pomegranate peel, beet leaf, and broccoli flower extracts on oxidative stability and physicochemical properties of cooked ground beef during refrigerated storage
Şimşek, S., & Kılıç, B. (2024) · Food Science & Nutrition · doi:10.1002/fsn3.4419 · Cited by 2
“The extraction of fats from cooked ground meat samples was carried out using the method explained by Bligh and Dyer (1959).”
Method
Development of Profiling Method of Monoacylglycerol Species Based on Liquid Chromatography Tandem Mass Spectrometry
Kawabata, K., Sato, Y., Tokumura, A., et al. (2024) · Chromatography · doi:10.15583/jpchrom.2024.010 · Cited by 2
Sensory acceptance of mango ice cream added with whey protein by consumers affected and not affected by cancer
Peres, A. P., dos Santos, E. B., Morzelle, M. C., et al. (2024) · Brazilian journal of food technology/Brazilian Journal of Food Technology · doi:10.1590/1981-6723.00824 · Cited by 2
Water-soluble oat extract enriched with mango peel flour: preparation, characterization and application in Greek yogurt
GODOI, H. S. D., Raspe, D. T., Stevanato, N., et al. (2024) · Anais da Academia Brasileira de Ciências · doi:10.1590/0001-3765202420231353 · Cited by 2
3,5-Dimethyl-2,4,6-trimethoxychalcone Lessens Obesity and MAFLD in Leptin-Deficient ob/ob Mice
Gaigé, S., Abysique, A., Barbouche, R., Tonetto, A., Maio, A. D., Robin, M., Lormier, A.-T., & Troadec, J. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25189838 · Cited by 2
“Two-way ANOVAs followed by Bonferroni’s multiple comparisons was used to assess effects at different times post treatment (Figure 6A,C). p -values less than 0.05 were considered significant.”
MethodInfluential
Direct analysis by ultra-high-resolution mass spectrometry of lipid A and phospholipids from Acinetobacter baumannii cells
Vergoz, D., Schaumann, A., Schmitz‐Afonso, I., et al. (2024) · Biochimie · doi:10.1016/j.biochi.2024.09.012 · Cited by 2
From ice to neurons: investigating the neuroprotective effects of Antarctic microalgae Chlorella variabilis and Chlorella pyrenoidosa extracts
İnan, B., Mutlu, B., Çakır-Koç, R., et al. (2024) · 3 Biotech · doi:10.1007/s13205-024-04094-4 · Cited by 2
“Carbohydrate, lipid and protein contents of the polar micro-algae were detected by the phenol–sulfuric acid method (Dubois et al. 1956), the Bligh–Dyer method (Bligh and Dyer 1959) and the Lowry method (Lowry et al. 1951), respectively.”
Valorization of Biodiesel-Derived Crude Glycerol for Producing Yeast Oil as an Alternative Biodiesel Production Feedstock: Productivity Improvement in Batch and Repeated Batch Fermentation
Rakkitkanphun, C., Teeka, J., Chiu, C.-H., et al. (2024) · Waste and Biomass Valorization · doi:10.1007/s12649-024-02725-8 · Cited by 2
“A modified method of Bligh and Dyer was used to extract the microbial oil, which was measured as a gram of total oil per liter of fermentation broth (g/L) [36].”
Differences in phytoplankton population vulnerability in response to chemical activity of mixtures
dos Anjos, T. B. O., Nham, Q., Abel, S., Lindehoff, E., Bradshaw, C., & Sobek, A. (2024) · Environmental Science Processes & Impacts · doi:10.1039/d4em00249k · Cited by 2
Effect of the extraction method on the lipid composition of purslane (Portulaca oleracea L.) seed oil
Taneva, S., & Momchilova, S. (2024) · Discover Food · doi:10.1007/s44187-024-00185-6 · Cited by 2
“The procedure of Bligh & Dyer [12] was used as follows: portion of 10 g (precisely weighed) milled purslane seeds were homogenized with 200 ml chloroform–methanol mixture (1:2, v/v) in an Erlenmeyer flask with magnetic stirrer for 3 h.”
Online Direct Infusion Mass Spectrometry of Liquid–Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe
Marques, C., Blaase, L., & Lanekoff, I. (2024) · Metabolites · doi:10.3390/metabo14110587 · Cited by 4
Unraveling the Potential of Yarrowia lipolytica to Utilize Waste Motor Oil as a Carbon Source
Miranda, S. M., Belo, I., & Lopes, M. (2024) · Journal of Fungi · doi:10.3390/jof10110777 · Cited by 2
“Total lipids in the WMO were determined gravimetrically, in triplicate, after extraction with chloroform and methanol (adapted from Bligh and Dyer [32]).”
The Onset of Steatosis Occurs as Early as Seven Days and Progresses to Nonalcoholic Steatohepatitis in a Pediatric Pig Model of Nonalcoholic Fatty Liver Disease
Yadav, R., Gerrard, S. D., Lima, M. R. M., et al. (2024) · Journal of Nutrition · doi:10.1016/j.tjnut.2024.11.009 · Cited by 2
Sirtuin-5 Is Recruited to Hepatic Peroxisomes in Mice Fed Dodecanedioic Acid but Has Little Impact on the Peroxisomal Succinylome
Zhang, Y., Zhang, B. B., Bharathi, S. S., et al. (2024) · Biomolecules · doi:10.3390/biom14121508 · Cited by 2
Transcriptome-guided breeding for Paspalum notatum: producing apomictic hybrids with enhanced omega-3 content
Marino, L., Altabe, S., Colono, C. M., et al. (2024) · Theoretical and Applied Genetics · doi:10.1007/s00122-024-04788-6 · Cited by 2
“Lipid extraction was performed following the method published by Bligh and Dyer (1959).”
Development and characterization of a nut-based beverage of Brazil nut (Bertholletia excelsa) and macadamia (Macadamia integrifolia)
Machado, A. L. B., SILVA, K. S., Miranda, B. M., et al. (2024) · Food Science and Technology · doi:10.5327/fst.02223 · Cited by 2
Mass Spectrometry-Based Non-Targeted Lipidome Analysis and Extraction of Markers for the Authentication of White and Black Truffle Species and Their Origin Determination
Tejedor‐Calvo, E., Marco, P., Fischer, M. J., et al. (2024) · Agriculture · doi:10.3390/agriculture14122350 · Cited by 2
The effects of expanded polystyrene particle on energy metabolism of the sea slater (Ligia cinerascens) originating from a highly EPS-polluted area
Lee, Y.-H., Yoo, J.-W., Jung, J.-H., & Lee, Y.-M. (2024) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2024.110118 · Cited by 2
Chemical Analysis, Antioxidant Activity, and in vitro Digestibility of Crataegus azarolus Leaves and Fruits in Goats
Sammari, H., Jedidi, S., Abidi, A., et al. (2024) · Natural Product Communications · doi:10.1177/1934578x241309741 · Cited by 2
Effects of size and amount and burial depth on early decomposition of maize straw and the links to microbial and nematode communities in the Mollisol of China
Zhang, F., Wang, T., Jiang, H., Zhang, B., Han, Y. F., & Yao, S. (2024) · Soil Use and Management · doi:10.1111/sum.70003 · Cited by 3
Improved fatty acid extraction from microalgae with a novel combined process pulsed electric field-supercritical CO₂ extraction
Thabit, H. M. (2024) · Wasit Journal of Pure sciences · doi:10.31185/wjps.568 · Cited by 2
“Such mechanical disruption methods have varying efficiencies according to the microalgae species, and how their cell wall is built [19].”
Influential
Lactose-assimilating yeasts with high fatty acid accumulation uncovered by untargeted bioprospecting
Persson, K. O., Onyema, V. O., Nwafor, I. P., Peri, K. V. R., Otti, C., Nnaemeka, P., Onyishi, C. L., Okoye, S., Moneke, A. N., Amadi, O. C., et al. (2024) · Applied and Environmental Microbiology · doi:10.1128/aem.01615-24 · Cited by 2
Quality parameters and storage stability of the Norwegian red sea cucumber (Parastichopus tremulus)
Vu, D. T., Tsoukalas, D. S., Jakobsen, A. N., et al. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.105846 · Cited by 2
Hydrogenation of biomass-derived fatty acid esters and Dunaliella salina catalyzed by Pd on V2O5
Pandey, M., Rai, A., Prasad, N., Mohanta, A., Asthana, R. K., & Ranganath, K. V. S. (2025) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-025-06490-z · Cited by 2
“The lipid was extracted and the total content (% dry weight) was quantified gravimetrically following the modified method of Bligh and Dyer [26].”
Patterns of variations in lipid molecular profile during larval development of red king crab, Paralithodes camtschaticus, and Japanese mitten crab, Eriocheir Japonica
Ermolenko, E. V., Sikorskaya, T. V., Grigorchuk, V. P., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-85901-7 · Cited by 2
Bioactive compounds and composition of Elaeis oleifera mesocarp oil extracted by hydraulic pressing
Antoniassi, R., Wilhelm, A. E., Guedes, A. M. M., et al. (2024) · Grasas y Aceites · doi:10.3989/gya.0646241.2194 · Cited by 2
“(1990), reported a higher content of carotenoids (1577 µg/g) for the oil from Eo and up to 6-fold than Eg, although the extraction was performed by the Bligh and Dyer method (Bligh and Dyer, 1959).”
Alpha 1-antitrypsin mitigates salt-sensitive hypertension in juvenile mice by reducing diacylglycerol concentrations and protein kinase C activity in kidney membranes
Dogan, Y. E., Bala, N., Galban, E. S., et al. (2025) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2024.1485506 · Cited by 2
Effect of nitrogen limitation and irradiance on the biochemical composition of Haslea ostrearia
Monge, H. B., & Sánchez‐Saavedra, M. D. P. (2025) · Algal Research · doi:10.1016/j.algal.2025.103931 · Cited by 2
Are commercial plant extracts efficient antioxidants in cooked and frozen beef, pork, chicken and mechanically deboned chicken meat patties?
Paglarini, C. D. S., Vidal, V. A. S., Neri-Numa, I. A., et al. (2025) · Food and Humanity · doi:10.1016/j.foohum.2025.100514 · Cited by 2
Probiotic-based adsorbent mitigates aflatoxin B1 toxicity in Piaractus mesopotamicus: assessing well-being via changes in tissue architecture and digestive enzyme activity
Ziemniczak, H. M., Conceição, L. M. A., Godoy, A. C., Neu, D. H., Rodrigues, A. T., de Campos, C. M., Acunha, R. M. G., Gandra, J. R., Saturnino, K. C., Pereira, U. D. P., et al. (2025) · Veterinary Research Communications · doi:10.1007/s11259-025-10663-6 · Cited by 2
“Total lipids were determined following the meth - odology of Bligh and Dyer (1959).”
Corynebacterium mayonis sp. nov. isolated from a human blood culture
Mohammed, H. T., Koscianski, C. A., Berent, T. E., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006632 · Cited by 2
Microbial phosphorus in loamy, basalt-derived forest soil is altered by plant nutritional status: a root-splitting study
Butler, O. M., & Warren, C. R. (2025) · Plant and Soil · doi:10.1007/s11104-025-07224-w · Cited by 2
“:P ratios of S. glomulifera seedlings were strongly affected was subjected to the Bligh and Dyer lipid extraction protocol with modifications to optimise the extraction for soils (Bligh and Dyer 1959; Warren 2018).”
Lipid Curvature and Fluidity Influence Lipid Incorporation Disparities in Nanodiscs
Sarcinella, M. C., Jones, J. D., Sorensen, M. J., et al. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c05494 · Cited by 2
Stable isotope analysis reveals individual variations in feeding habits among wild Japanese macaques
Funakawa, K., Suzumura, T., & Koba, K. (2025) · Ecological Research · doi:10.1111/1440-1703.12547 · Cited by 2
Genomic insights into Marinospirillum alkalitolerans sp. nov., a novel PHB producing bacterium from an Indian impact crater, and an emended description of family Oceanospirillaceae
Joshi, A. A., Thite, S., Godbole, D., et al. (2025) · Antonie van Leeuwenhoek · doi:10.1007/s10482-024-02059-4 · Cited by 2
“Bacterial strains MEB164 T , MEB148, M. alkaliphilum DSM 21637 T , and M. celere DSM 18438 T in their logarithmic growth phase, were subjected to polar lipid extraction following the protocol described by Bligh and Dyer (1959), with modifications as outlined by Card (1973).”
Application of microfluidics for revealing physiological metabolic response of algae at the single-cell level
Qiao, Y.-F., Li, S., Liu, T., Chen, Y., Guo, J., Liu, S., & Yan, P. (2025) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2025.115867 · Cited by 2
Ontogenetic and sex variation in the foraging ecology of the salmon shark (Lamna ditropis) in the California current ecosystem
McInturf, A. G., Teixeira, C. R., Boyt, R. B. R., et al. (2025) · Marine Biology · doi:10.1007/s00227-025-04602-x · Cited by 2
“For liver samples, which have higher lipid content, we used a 2:1 chloroform:methanol solution (Bligh and Dyer 1959 ; MacNeil et al. 2005), followed by deionized water rinses to eliminate urea.”
Soil carbon responses to prescribed burning, nitrogen addition, and their interactions in a Mediterranean shrubland
Inclán, R. M., Pérez-Pastor, R. M., Clavero, M. A., Cicuéndez, V., Cobos, J., Remedios, E., & Sánchez-Ledesma, D. M. (2025) · Biogeochemistry · doi:10.1007/s10533-025-01212-z · Cited by 2
“Soil microbial communities were analysed using the microbial phospholipid fatty acid (PLFAs) method (Bligh and Dyer 1959).”
Effect of different sodium nitrate concentrations on the growth, biomass, and biochemical composition of Tetraselmis chuii
Fakhri, M., Amrulloh, A. A., Yuniarti, A., Supriatin, F. E., & Arifin, N. B. (2025) · Journal of Aquaculture and Fish Health · doi:10.20473/jafh.v14i1.64564 · Cited by 3
“Total lipids were extracted following the protocol of Bligh and Dyer (1959) and Feng et al .”
Microalgal mixotrophic cultivation enhances lignocellulosic pretreatment wastewater utilization: Inhibitors detoxification process and C5/C6 sugar high-value conversion
Wang, H., Qin, L., Qi, W., et al. (2025) · Algal Research · doi:10.1016/j.algal.2025.104007 · Cited by 2
Exploring side streams upcycling for crickets farming: Insects biology and chemical composition
Fernandes, A. P., Cazarolli, L. H., Pigatto, T., et al. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.106431 · Cited by 2
Absolute Quantification of Individual Phospholipids in Infant Formula using Phosphorus Nuclear Magnetic Resonance Spectroscopy
Kato, T., Yamamoto, K., Naito, S., et al. (2025) · Journal of Oleo Science · doi:10.5650/jos.ess24232 · Cited by 2
Diet-induced obesity dampens the temporal oscillation of hepatic mitochondrial lipids
Jain, R., Rajendran, R., Rajakumari, S., & Rajakumari, S. (2025) · Journal of Lipid Research · doi:10.1016/j.jlr.2025.100790 · Cited by 3
“Lipids were extracted from isolated liver mitochondria (n = 3 biological replicates per ZT point, where each biological replicate was generated by pooling liver tissue from two mice) using the modified Bligh and Dyer method (39).”
Optimization of Almond Beverage Enriched with Omega-3 Fatty Acids by Adding Brown Flaxseeds (Linum usitatissimum L.) Using D-Optimal Mixing Diagram Method
Hussein, Z. E. H., da Silva, J. M., Castro, M. C., et al. (2025) · Food Technology and Biotechnology · doi:10.17113/ftb.63.02.25.8460 · Cited by 2
“Lipids were extracted from the samples according to the method developed by Bligh and Dyer ( 34 ), which was carried out in triplicate.”
Influence of arsenic exposure on the daily changes of glycerophospholipid turnover and assessment of defence mechanisms in tobacco hairy roots
Gutierrez, S., Ibáñez, S. G., Agostini, E., et al. (2025) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2025.109880 · Cited by 2
Interaction Between Organic Carbon Conversion and Microbial Metabolism in Marginal Seas Sediments During Hydrodynamic Sorting Processes
Yang, Z., Xing, L., Che, Y., Wang, N., Ma, H., Zhao, S., Chen, A., Li, H., & Bao, R. (2025) · Journal of Geophysical Research Oceans · doi:10.1029/2024jc021994 · Cited by 2
Effects of different dietary protein sources on the growth performance, digestive capacity and amino acid profiles of largemouth bass (Micropterus salmoides) larvae
Sun, S., You, Q., Wang, P., et al. (2025) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2025.116343 · Cited by 2
Investigation on the Lipid Profile of Ripened Pu‐erh Tea and Relationships Between Their Changes and Key Aromatic Volatiles
Li, W., Yi, L., Gu, Y., et al. (2025) · Journal of Separation Science · doi:10.1002/jssc.70133 · Cited by 2
Correlation between trace element concentrations in the blood of female hawksbill (Eretmochelys imbricata) and egg quality in nesting populations of São Tomé Island
Morão, I. F. C., Simões, T., Casado, R. B., Vieira, S., Ferreira‐Airaud, B., Caliani, I., Noi, A. D., Casini, S., Fossi, M. C., Lemos, M. F. L., et al. (2025) · Environmental Research · doi:10.1016/j.envres.2025.121594 · Cited by 2
Phosphatidic acid phosphatase LPIN1 in phospholipid metabolism and stemness in hematopoiesis and AML
Huber, K., Garg, S., Schlautmann, L. P., Wang, R., He, L., Huth, R., Pouya, A., Rohde, C., Janssen, M., Lüchtenborg, C., et al. (2025) · HemaSphere · doi:10.1002/hem3.70118 · Cited by 2
“Snap frozen cells were subjected to lipid extractions using an acidic liquid – liquid extraction method, 28 except for plasmalogens, which were extracted under neutral conditions.”
Unexpected 16S rRNA heterogeneity in ‘Acetobacterium dehalogenans’ and reclassification as Acetobacterium malicum subsp. dehalogenans subsp. nov.
Spring, S., Wolf, J., Kirstein, S., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006783 · Cited by 2
“Polar lipids were extracted from freeze-dried biomass using a modified Bligh and Dyer extraction [17].”
Exploring the bioremediation potential of nonpathogenic Pb-resistant strain Klebsiella pneumoniae in Oreochromis niloticus
Zafar, S., Ashraf, A., Asad, F., et al. (2025) · Bioremediation Journal · doi:10.1080/10889868.2025.2503454 · Cited by 2
“The dried fishes were grinded into fine powder which was subjected to calculate the moisture, dry matter, protein, nitrogen free extract, lipid, ash and gross energy (Bligh and Dyer 1959).”
Mercury compound-specific stable isotope fractionation in high-altitude lake ecosystems of the Bolivian Altiplano
Masbou, J., Point, D., Bouchet, S., et al. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.179630 · Cited by 2
Single-cell oil production by Rhodotorula mucilaginosa CCT 7688 employing soybean hull hydrolysate as a carbon and nutrient source
da Rocha, K. R., Roesler, B. C. S., & Burkert, C. A. V. (2025) · Brazilian Journal of Chemical Engineering · doi:10.1007/s43153-025-00569-w · Cited by 2
Effects of Coconut Exocarp Flavonoid and EDTA-2Na on Aldehyde Generation During Pan-Frying Processing of Squid (Dsidicus gigas)
Wang, X., Lin, H., Zhao, M., et al. (2025) · Foods · doi:10.3390/foods14111925 · Cited by 2
“Lipids were extracted from lyophilized powder of squid based on the Bligh–Dyer method [25].”
Description of Catenibacterium mitsuokai subsp. tridentinum subsp. nov., an anaerobic bacterium isolated from human faeces, and emended description of C. mitsuokai
Ricci, L., Selma‐Royo, M., Golzato, D., Servais, C., Nabinejad, A., Marchi, P., Punčochář, M., Trenti, F., Garcia‐Aloy, M., Armanini, F., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006798 · Cited by 3
“Overnight YCFA-GSM broth cultures were pelleted by centrifugation (4,000 r.p.m., 5 min), and cells were washed twice with PBS. Total lipids were extracted and purified [26] and analysed using the Acquity UPLC CSH-C18 column (2.1×100 mm, 1.7 µm; Waters, Milford, MA, USA).”
Influential
Development of the Quantification of Fatty Acid Species without Derivatization and Application to Their Profiling in Sera of Different Mammalian Species
Kawabata, K., OHTSUKI, Y., MURAOKA, H., et al. (2025) · Chromatography · doi:10.15583/jpchrom.2025.005 · Cited by 2
Endogenous Aquaporin-0 Lipid Binding in Ocular Lens Tissue via Native Mass Spectrometry
O’Neale, C. V. T., Harvey, S. R., Chetyrkin, S. V., et al. (2025) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.4c00500 · Cited by 2
Sphingosylphosphorylcholine is a substrate for the Pseudomonas aeruginosa phospholipase C/sphingomyelinase, PlcH
DiGianivittorio, P., Schutz, K. C., Hinkel, L. A., et al. (2025) · Microbiology Spectrum · doi:10.1128/spectrum.00972-25 · Cited by 2
“After incubation, lipids were extracted using the Bligh and Dyer method (58) and were prepared as described in the TLC section.”
Cholesteryl esters and high protein-to-lipid ratios distinguish non-vesicular extracellular particles from extracellular vesicles
Ghosal, S., Leporati, R., Varga, Á., Susánszki, P., Fekete, N., László, T., Bárkai, T., Grébecz, F. K., Khamari, D., Magyar, T. Z., et al. (2026) · Cell Communication and Signaling · doi:10.1186/s12964-026-02880-6 · Cited by 2
“For 167k-NVEPs, which lack a membrane for Annexin V binding, we utilized the reported marker HSP90ß [8, 35], as 167k-NVEP single positive annexin-V clusters was significantly lower than 100k-sEV (Fig.”
Synaptamide regulates astroglial functional activity after cerebral cortex injury
Manzhulo, I., Egoraeva, A. A., Ivashkevich, D., Manzhulo, O. S., & Ponomarenko, A. I. (2025) · Inflammopharmacology · doi:10.1007/s10787-025-01834-7 · Cited by 3
Oceaniferula spumae sp. nov., a novel Verrucomicrobiota bacterium, isolated from sea foam at Noto Peninsula, Ishikawa, Japan
Huang, C., Jiang, C., Sato‐Takabe, Y., Makabe‐Kobayashi, Y., Tsukamoto, Y., Yoshizawa, S., & Hamasaki, K. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006828 · Cited by 2
Signals from the sea: the structural peculiarity of lipid A and weak immunostimulatory lipopolysaccharide from Rheinheimera japonica
Chiara, S. D., Olmeo, F., Andretta, E., et al. (2025) · RSC Chemical Biology · doi:10.1039/d5cb00134j · Cited by 2
Industrial-scale valorization of decolorized soy sauce wastewater into sustainable and cost-effective microalgal biodiesel
Hwang, I. H., Kwak, H. S., Chun, Y., Lee, J. S., & Sim, S. J. (2025) · Algal Research · doi:10.1016/j.algal.2025.104198 · Cited by 2
Combining 13C, 15N, and 2H tracers to measure feeding and metabolic activity in marine, shallow-water sponges – A pilot study
Stratmann, T., Sahonero‐Canavesi, D. X., & van der Meer, M. T. J. (2025) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2025.152123 · Cited by 2
Optimization of the Production of Press Cake Paste From Apricot ( Prunus armeniaca L.) Kernels and Prediction of Shelf‐Life Kinetics of the Product
Turan, B. K., Kanmaz, H., & Hayaloğlu, A. A. (2025) · Journal of Food Science · doi:10.1111/1750-3841.70416 · Cited by 2
A cannabinoid receptor 1 inverse agonist induces weight loss and reduces airway hyperresponsiveness in a mouse model of obese asthma
Morris, C. R., Chandrasekaran, R., Butzirus, I. M., Daphtary, N., Aliyeva, M., Manuel, A. M., Tharp, W. G., Bates, J. H. T., Anathy, V., Poynter, M. E., et al. (2025) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00049.2025 · Cited by 2
Anti‑neuroinflammatory potential of porcine liver decomposition products in improving behavioral abnormalities: Effects on formalin‑ and LPS‑induced inflammation
Sato, I., Maeda, T., Katsuyama, S., et al. (2025) · Biomedical Reports · doi:10.3892/br.2025.2032 · Cited by 2
“The components of PLDP are listed in Table I. PEL was extracted from PLDP using the Bligh & Dyer method (13).”
Effects of Adding Hydroxytyrosol to the Diet of Pigs in the Nursery Phase on Growth Performance, Biochemical Markers, and Fatty Acid Profile
Rofino, R. D. A., Ficagna, C. A., Zamboni, T., Klein, B., Altieri, E. A., O’Connor, K. E., Davis, R., Walsh, M., Tavernari, F. D. C., Boiago, M. M., et al. (2025) · Animals · doi:10.3390/ani15152268 · Cited by 2
“…samples, the profile of deposited fatty acids was analyzed, and the extraction of fatty acids was carried out using the method of researchers [20]; 2.8 g of meat samples, 4.3 mL of water, 16 mL of methanol, and 8 mL of chloroform were added in a 50 mL polypropylene tube and homogenized in…”
Influential
Insect Lipidomics: Advances, Applications, and Physiological Insights
Musselman, L. P., Cedden, D., Güney, G., & Toprak, U. (2025) · Advances in experimental medicine and biology · doi:10.1007/5584_2025_878 · Cited by 2
Ablation of Hepatocyte Derived‐ FGL1 Does Not Aggravate Metabolic Dysfunction‐Associated Steatotic Liver Disease
Personnaz, J., Cannizzo, L., Martin, C. M. P., Desquesnes, A., Sotin, M., DaSilva, J., Perrier, P., Guillou, H., & Kautz, L. (2025) · The FASEB Journal · doi:10.1096/fj.202501997r · Cited by 1
“Lipids from 1 mg of liver weight were extracted using the adapted Bligh and Dyer method [26] in dichloromethane/ methanol (2% acetic acid)/water (2.5:2.5:2 v/v/v).”
Influential
The Evolving Landscape of Analytical Mitochondrial Research: Mass Spectrometry, Its Proteomic and Lipidomic Insights
Dhakne, P., Aher, D., Bhutada, R., & Sengupta, P. (2025) · Critical Reviews in Analytical Chemistry · doi:10.1080/10408347.2025.2545295 · Cited by 2
“Classical methods for extraction of lipids were reported by Floch [62] and Bligh & Dyer [63] in 1957 and 1959, respectively.”
Consumption of a Mediterranean lean fish enriched with platelet-activating factor inhibitors extracted from olive pomace favorably modulates hemostasis and thrombosis in healthy adults with overweight
Antonopoulou, S., Detopoulou, M., Petsini, F., et al. (2025) · Food & Function · doi:10.1039/d5fo02487k · Cited by 2
Obeticholic acid does not restore Western diet-induced alterations in hepatic mitochondrial respiration
Melek, J., Staňková, P., Peterová, E., Maseko, T. E., Špalková, V., Matar, R., Ibrahim, A., Elkalaf, M., Podhola, M., Štefela, A., et al. (2025) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2025.118542 · Cited by 2
Structure and solubility of pea seed-derived ingredients produced by air classification: Relevance of the seed milling process and endogenous lipid accumulation
Gélébart, P., Maraval, G., Kermarrec, A., Ribourg-Birault, L., Geairon, A., Meynier, A., Riaublanc, A., Réau, A., & Berton‐Carabin, C. (2025) · Food Research International · doi:10.1016/j.foodres.2025.117556 · Cited by 2
Effects of sodium hypochlorite disinfection on cultivation of Auxenochlorella pyrenoidosa, Phaeodactylum tricornutum, and Chaetoceros sp.: Physiological, biochemical, and transcriptomic responses
Wang, Q., Ji, H., & Zhang, L. (2025) · Algal Research · doi:10.1016/j.algal.2025.104355 · Cited by 2
Environmental Controls on Crenarchaeol Distributions in Hydrothermal Springs
Calhoun, A., Blewett, J., Colman, D. R., Amenábar, M. J., Harris, C. M., Boyd, E. S., Pearson, A., & Leavitt, W. D. (2026) · Environmental Microbiology · doi:10.1111/1462-2920.70248 · Cited by 2
“Sulphide and Fe (II) concentrations were measured on water samples collected by a high-density polyethylene sampling staff (turbid water samples were filtered through pre-sterilised 0.22 μm Sterivex filters (EMD Millipore, Billerica, MA, USA)), whilst all other parameters were measured in situ near the site of sedimen…”
Influential
SCREENING AND GROWTH KINETIC STUDIES OF WILD CHLOROPHYCEAN FRESH WATER MICROALGAL SPECIES FOR BIOMASS AND BIOFUEL APPLICATIONS
Kumar, S., Pareek, A., & Mukhopadhyay, K. (2014) · International Journal of Pharmacy and Pharmaceutical Sciences · Cited by 2
Confirming the origin of wild and farmed fish
Lees, M., & Thomas, F. (2008) · Elsevier eBooks · doi:10.1533/9781845694920.3.565 · Cited by 2
In vitroeffects of phosphatidylcholine andtransgalactooligosaccharides on the production of 1,2-sn-diacylglycerol byBifidobacterium longumbiovarinfantis
Vulevic, J., & Gibson, G. R. (2008) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2008.03886.x · Cited by 2
Changes in Proximate Composition and Lipid Components in Chub Mackerel Scomber japonicus and Japanese Jack Mackerel Trachurus japonicus with Various Cooking Methods
Moon, S.-K., Kang, J.-Y., Kim, I.-S., et al. (2013) · Korean Journal of Fisheries and Aquatic Sciences · doi:10.5657/kfas.2013.0708 · Cited by 2
Composição proximal e perfil de ácidos graxos do lambari-do-rabo-vermelho (Astyanax fasciatus) de diferentes classes de peso
Furuya, V. R. B., Furuya, W. M., Michelato, M., et al. (2013) · Revista Brasileira de Saúde e Produção Animal · doi:10.1590/s1519-99402013000400020 · Cited by 2
Cholesterol and alpha-tocopherol contents of fish and other seafood from the Straits of Malacca
Azlan, A., Aziz, N. A., Khoo, H. E., et al. (2015) · Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) · Cited by 2
Caracterização químico-nutricional de um isolado protéico de soro de leite, um hidrolisado de colágeno bovino e misturas dos dois produtos Chemical-nutritional characterization of a whey protein isolate, a bovine collagen hydrolysate and mixtures of the two products
Sgarbieri, V. C. (2009) · Cited by 2
Increase of the Plasma Trans-fatty Acid Level in Middle-aged Japanese Men with Non-alcoholic Fatty Liver Disease
Tabuchi, M., Tomioka, K., Hiramatsu, M., et al. (2014) · Nippon Eiyo Shokuryo Gakkaishi · doi:10.4327/jsnfs.67.137 · Cited by 2
Fatty Acid Composition of Cultured Fibroblasts Derived from Healthy Nasal Mucosa and Nasal Polyps
Ayyad, S. J., Roca‐Ferrer, J., & Picado, C. (2016) · Sinusitis · doi:10.3390/sinusitis1010055 · Cited by 2
Existence of cis–Vaccenic Acid in Rice Vinegar Lipids and Its Origin
FUJIMORI, M., Yunoki, K., Hidetsugu, G. O. T. O., et al. (2009) · JOURNAL OF THE BREWING SOCIETY OF JAPAN · doi:10.6013/jbrewsocjapan.104.123 · Cited by 2
Apparent Digestibility and Utilization of Protein and Phosphorus in diets of incorporated with Sprouted Sorghum, Phytase and Protease Enzymes for African Catfi sh (Clarias gariepinus)
Kemigabo, C. (2017) · International Journal of Aquaculture and Fishery Sciences · doi:10.17352/2455-8400.000033 · Cited by 2
Non-enzymatic conversion of primary oxidation products of Docosahexaenoic acid into less toxic acid molecules
Narayanankutty, A., Gopinath, M. K., Vakayil, M., et al. (2018) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2018.05.082 · Cited by 2
Proximate and Macro Element Composition of Four Fish Species from Lower River Benue Makurdi Benue State Nigeria
Ihie, I. S., Gabriel, S. S., & Jo, E.-I. (2018) · Journal of Nutritional Health & Food Science · doi:10.15226/jnhfs.2018.001127 · Cited by 2
Qualitative and Quantitative Estimation of Bacterial Lipid Production
Muralitharan, G., Gayathri, M., & Shunmugam, S. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9484-7_4 · Cited by 2
Evaluation of Bacterial Lipid Production: Quantitative and Qualitative Measurements: Tips and Guidelines
Modiri, S., Zahiri, H. S., & Noghabi, K. A. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9484-7_23 · Cited by 2
Carcass characteristics and fatty acid profile of Santa Inês lamb fed banana leftovers
de Menezes, A. M., Tanure, C. B., Peripolli, V., et al. (2019) · Scientia Agricola · doi:10.1590/1678-992x-2017-0379 · Cited by 2
“Lipids from Longissimus lumborum muscle were extracted using the method described by Bligh and Dyer (1959) and Hartman and Lago (1973).”
Method
Effects of probiotic supplementation and postmortem storage condition on the oxidative stability of M. Pectoralis major of laying hens
Xue, S., Hu, J., Cheng, H. W., et al. (2019) · Poultry Science · doi:10.3382/ps/pez518 · Cited by 2
The Chlamydial Inclusion
Hybiske, K., & Heuer, D. (2019) · Caister Academic Press eBooks · doi:10.21775/9781912530281.04 · Cited by 2
LIPID COMPOSITION AND MEMBRANOPROTECTIVE ACTION OF EXTRACT FROM MARINE GREEN ALGAE ULVA LACTUCA (L.)
Евгеньевна, Ф. С., Kushnerova, N. F., Sprygin, V. G., Drugova, E. S., Lesnikova, L. N., & Merzluakov, V. Y. (2019) · chemistry of plant raw material · doi:10.14258/jcprm.2019035116 · Cited by 2

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks