Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“Extracts were partitioned according to Bligh and Dyer (1959) using a 1:1:0.9 solution of DCM, methanol, and NaCl (20% aqueous solution) to separate organic extracts from the aqueous phase.”
Influential
Effects of microalgae inoculated diet on growth performance and blood parameters of Nile tilapia (Oreochromis niloticus)
Amira, K. I., RAHMAN, M. R., Khatoon, H., Sıkder, S., Islam, S. A., Afruj, J., JAMAL, F., & Haque, M. E. (2022) · Bangladesh Journal of Fisheries · doi:10.52168/bjf.2021.33.27 · Cited by 2
“Method provided by Bligh and Dyer (1959) was followed to determine the lipid content of microalgae.”
Method
Functional divergence of a pair of Arabidopsis phospho‐base methyltransferases, PMT1 and PMT3 , conferred by distinct N‐terminal sequences
Liu, Y., Tan, Y. R., Chang, C., et al. (2022) · The Plant Journal · doi:10.1111/tpj.15741 · Cited by 2
Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum
Elsaba, Y. M., Boroujerdi, A. F. B., & Abdelsalam, A. (2022) · Mycobiology · doi:10.1080/12298093.2022.2059889 · Cited by 2
“between dry mass and water loss [35,36] using 2:2:1”
Method
Biochemical analysis and evaluation of free radical scavenging activity of bitter gourd seeds' aqueous and ethanolic extracts: Comparative study
Saha, M., & Chatterjee, S. (2022) · Journal of Medicinal Plants Studies · doi:10.22271/plants.2022.v10.i1b.1369 · Cited by 2
“Quantitative Screening of lipid The total lipid content was estimated using the standard method ([30]).”
Method
Production of Two Types of Exopolysaccharide by Novosphingobium rosa.
Matsuyama, H., Kamesaki, T., Sasaki, R., et al. (2003) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.95.152 · Cited by 2
Maradolipids: Diacyltrehalose Glycolipids Specific to Dauer Larva in Caenorhabditis elegans
Penkov, S., Mende, F., Zagoriy, V., et al. (2010) · Angewandte Chemie · doi:10.1002/ange.201004466 · Cited by 2
Effects of Ursodeoxycholic Acid on Growth Performance and Digestive Enzyme Activities of Juvenile Red Sea Bream, Pagrus major
Alam, M. S., Teshima, S., Ishikawa, M., et al. (2001) · Aquaculture Science · doi:10.11233/aquaculturesci1953.49.53 · Cited by 2
A sensitive method for quantification of aceticlastic methanogens and estimation of total methanogenic cells in natural environments based on an analysis of ether-linked glycerolipids
Ohtsubo, S. (1993) · FEMS Microbiology Ecology · doi:10.1016/0168-6496(93)90023-z · Cited by 2
TRANSVERSE DISTRIBUTION OF PHOSPHOLIPIDS IN THE VERTEBRATE PHOTORECEPTOR MEMBRANE
Bonting, S. L., Drenthe, E. H. S., & DAEMEN, F. J. M. (1980) · Elsevier eBooks · doi:10.1016/b978-0-08-025485-2.50004-3 · Cited by 2
Omega 3 and Omega 6 Fatty Acids
Stark, K. D. (2012) · CRC Press eBooks · doi:10.1201/b11653-43 · Cited by 2
Measurement of Phospholipid Metabolism in Intact Neutrophils
Sergeant, S., & McPhail, L. C. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-845-4_7 · Cited by 2
Suspect screening of exogenous compounds using multiple reaction screening (MRM) profiling in human urine samples
Júnior, C. A. M., Edwards, M. E., Lamarca, R. S., et al. (2022) · Journal of Chromatography B · doi:10.1016/j.jchromb.2022.123290 · Cited by 2
Effect of Superoxide dismutase on cryopreservation of mithun semen
Ponraj, P., De, A. K., VIKRAM, R., et al. (2022) · The Indian Journal of Animal Sciences · doi:10.56093/ijans.v92i6.116624 · Cited by 2
Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci
Кононова, Л. И., Lemkina, L. M., & Korobov, V. P. (2022) · Бюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наук · doi:10.29413/abs.2022-7.3.9 · Cited by 2
Nutrient biomass production from agro-industrial residues using Yarrowia lipolytica: screening and optimization of growing conditions
Bitencourt, T. B., Souza, F. A., da Silva, V. G., et al. (2022) · Brazilian journal of food technology/Brazilian Journal of Food Technology · doi:10.1590/1981-6723.28720 · Cited by 2
Solid-liquid Extraction of Soluble Carbohydrates from Soybean Meal: an Experimental Study, Kinetics, and Modeling
Levek, C. A., Hamerski, F., Ribani, R. H., et al. (2022) · Brazilian Archives of Biology and Technology · doi:10.1590/1678-4324-2022210662 · Cited by 2
Challenges for microalgae cultivation in sugarcane processing wastewater (vinasse) for biodiesel production: From the bench to pilot scale
Bastos, R. G., da Silva, M. A., Mattos, L. F. A., et al. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-90971-6.00009-7 · Cited by 2
Dietary product based on sea urchin caviar and Sardinops melanostictus fat
Alrajab, M., & Васильевна, Ш. Л. (2022) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2022.100512 · Cited by 2
“Determination of the Fractional Composition of Lipids Lipids were extracted from the samples by the Bligh and Dyer method [30].”
Method
The Promising Future of Microalgae as Biofuels and Valuable Bioproducts
Dash, S., Parida, S., Sahoo, B., & Rath, B. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-19-0680-0_2 · Cited by 3
Binding of phenochalasin A, an inhibitor of lipid droplet formation in mouse macrophages, on G-actin
Kobayashi, K., Matsuda, D., Tomoda, H., & Ohshiro, T. (2022) · Drug Discoveries & Therapeutics · doi:10.5582/ddt.2022.01053 · Cited by 3
Intact Polar and Core Tetraether Lipids in Sediments from the Haiyang 4 Cold‐seep of the Northern South China Sea and their Implications
Yuejiao, L. I., Su, X., Jiao, L., et al. (2022) · Acta Geologica Sinica - English Edition · doi:10.1111/1755-6724.14928 · Cited by 2
“After freeze-drying and grinding, about 5 g of each sample was used for lipid extraction using the modified Bligh and Dyer protocol (Bligh and Dyer, 1959).”
Method
Physiological properties of Neoporphyra haitanensis conchocelis in response to different temperature
Wang, X., Fu, D., Hui, Z., et al. (2022) · Aquaculture Research · doi:10.1111/are.16067 · Cited by 2
Valuation of wastewater treatment and biodiesel production from microalgae by direct transesterification method
Taher, F., & Mahfouz, A. Y. (2022) · Egyptian Journal of Chemistry · doi:10.21608/ejchem.2022.132259.5818 · Cited by 2
Improved chemical and isotopic labeling of biomembranes in Bacillus subtilis by leveraging CRISPRi inhibition of beta-ketoacyl-ACP synthase (fabF)
Nickels, J. D., Bonifer, K. S., Tindall, R. R., et al. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2022.1011981 · Cited by 2
“, 2000) of the method of Bligh and Dyer (Bligh and Dyer, 1959).”
Method
The effects of feeding benzoic acid and/or active dry yeast (Saccharomyces cerevisiae) on fatty acid composition, sensory attributes, and retail shelf-life of beeflongissimus thoracis
Williams, M. S., Mandell, I. B., Wood, K. M., & Bohrer, B. M. (2022) · Translational Animal Science · doi:10.1093/tas/txac161 · Cited by 3
Diet supplementation with egg yolk powder fattens the beetle Tribolium castaneum
Moura‐Silva, J., Tavares, M. P. S., Almeida‐Oliveira, F., et al. (2023) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.22000 · Cited by 2

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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