Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Papagrigoriou, G., Papazoglou, D., Lazari, D. M., et al. (2019) · Industrial Crops and Products · doi:10.1016/j.indcrop.2019.02.021 · Cited by 1
Exploring the possibility of predicting long‐term oxidative stability in prototype skincare formulations using various lipid oxidation initiators
Thomsen, B. R., Taylor, R., Hermund, D. B., et al. (2019) · International Journal of Cosmetic Science · doi:10.1111/ics.12515 · Cited by 1
Fatty Acid Profiling of Polyethylene Glycol Adapted and Un-Adapted Cell Lines of Oryza sativa L.cv. Swat-1 under Temperature Stress
Shah, S. M. S., Ullah, F., & Shah, S. H. (2019) · American Journal of Molecular Biology · doi:10.4236/ajmb.2019.94011 · Cited by 1
FRACTIONAL COMPOSITION OF WATER-SOLUBLE PROTEIN COMPONENTS OF DRY FERMENTOLIZATE AND STARTER FEED FOR SALMON FISH
Bashtovoy, A., Karaulova, E. P., Николаевна, Т. Г., et al. (2019) · Izvestiya TINRO · doi:10.26428/1606-9919-2019-199-241-248 · Cited by 1
Fatty Acid Characterization of Beef Longissimus from Steers Finished on Fodder Beet or Traditional Winter Forages in New Zealand Compared to US Grain-Fed Beef
Milopoulos, J. T., Garmyn, A. J., Wilkinson, R. C., & Miller, M. F. (2020) · Meat and Muscle Biology · doi:10.22175/mmb.10357 · Cited by 1
Effects of Dietary Omega-3 Fatty Acids (DHA/EPA) and Vitamin E on Fatty Acid Profile of Common Carp, Cyprinus carpio Ovaries
Salih, O. A. (2021) · Biological and Applied Environmental Research · doi:10.51304/baer.2021.5.1.10 · Cited by 1
“The oil was extracted from common carp ovaries using the modified Bligh & Dyer (1959) organic solvent method after drying the samples with a dryer at a temperature of 30 °C until the moisture was completely removed from the samples.”
Method
Constrained principal component analysis with stochastically ordered scores for high-dimensional mass spectrometry data
Hyun, H. J., Kim, Y., Kim, S. J., et al. (2021) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2021.104376 · Cited by 1
Prolonged refrigeration does not alter isoprostanes concentration in human milk
Peila, C., Longini, M., Toni, A. L., et al. (2021) · The Journal of Maternal-Fetal & Neonatal Medicine · doi:10.1080/14767058.2021.2006626 · Cited by 1
“The Bligh–Dyer method was used to extract and separate water-soluble and lipophilic metabolites [10].”
Method
Glycolytic and oxidative muscles under acute glucose supplementation differ in their metabolic responses to fatty acyl-CoA synthetase gene suppression
Jung, Y. H., & Bu, S. Y. (2022) · Journal of Nutrition and Health · doi:10.4163/jnh.2022.55.1.70 · Cited by 1
Cell culture metabolomics and lipidomics
Alecu, I., Sosa‐Miranda, C. D., Sandhu, J. K., Bennett, S. A. L., & Čuperlović‐Culf, M. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85062-9.00012-x · Cited by 1
Ammonia Concentration in the Eluent Influences Fragmentation Pattern of Triacylglycerols in Mass Spectrometry Analysis
Velasco, M., Balgoma, D., & Montero, O. (2022) · Metabolites · doi:10.3390/metabo12050452 · Cited by 1
“Lipid extraction was carried out by the chloroform–methanol method [37], but dichloromethane:methanol (2:1 v/v) was used instead.”
Method
Effect of dietary addition of phenolic compounds from propolis on growth performance, carcass traits, and meat fatty acid profile of feedlot beef cattle
de Aguiar, S. C., Machado, E., Simioni, F. L., et al. (2022) · Semina Ciências Agrárias · doi:10.5433/1679-0359.2022v43n4p1653 · Cited by 1
Composition of the microbial community in long-term organic and conventional farming systems
Talve, T., Talgre, L., Toom, M., et al. (2022) · Zemdirbyste-Agriculture · doi:10.13080/z-a.2022.109.013 · Cited by 1
Generation of a ceramide synthase 6 mouse lacking the DDRSDIE C-terminal motif
Kim, J., Pewzner‐Jung, Y., Joseph, T., Ben‐Dor, S., & Futerman, A. H. (2022) · PLoS ONE · doi:10.1371/journal.pone.0271675 · Cited by 1

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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