Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 44,201–44,300 of 48,847

Effects of temperature on biofilm formation and resource recovery during anoxyphototrophic treatment of fuel‐synthesis wastewater
Shaikh, S., Gasmi, S., Luyt, A. S., et al. (2025) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.7919 · Cited by 1
Phosphorus fertilization of grasses with dairy processing waste materials and contributions of soil microorganisms towards phosphorus uptake
Velasco‐Sánchez, Á., Hu, Y., Sommers, S. G., et al. (2025) · Geoderma · doi:10.1016/j.geoderma.2025.117406 · Cited by 1
Structural Transition from Closed to Open for the Influenza A M2 Proton Channel as Observed by Proton-Detected Solid-State NMR
Mohr, S., Schattenberg, C. J., Utesch, T., Sawczyc, H., Chevelkov, V., Lange, S., Kozuch, J., Sun, H., & Lange, A. (2025) · Journal of the American Chemical Society · doi:10.1021/jacs.5c05111 · Cited by 2
Building a cell-factory in Crithidia fasciculata: a bio-sustainable system to produce high-value polyunsaturated fatty acids
Cerone, M., Major, L. L., & Smith, T. (2025) · Microbial Cell Factories · doi:10.1186/s12934-025-02760-7 · Cited by 1
“375 µL 2:1 (v/v) of MeOH: CHCl 3 solution was added for biphasic separation based on the following method described by Bligh-Dyer [49].”
An Integrated Platform for High-Throughput Extraction and Mass Spectrometry-Based Quantification of Cholesterol and Sphingosine
Lin, Y., Fang, Y., Gosztyla, M. L., et al. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c06628 · Cited by 1
Untargeted Lipidomics in Fabry Disease of Urine Samples by Low-Resolution Flow Injection Mass Spectrometry (ESI(±)-LTQ MS)
Foletto, R. A., Borges, A. S., Motta, L. C., Lyrio, M. V. V., Barretto, C. T., Campos, L. C. G., Filgueiras, P. R., Baraúna, V. G., & Romão, W. (2025) · ACS Omega · doi:10.1021/acsomega.5c00894 · Cited by 1
Chemical, biochemical and environmental DNA analysis of sediments from two seamounts of the deep Arabian Sea
Raj, D., Anas, A., Jasmin, C., et al. (2025) · Gondwana Research · doi:10.1016/j.gr.2025.06.013 · Cited by 1
Fatty acid binding protein 1 (FABP1) depletion promotes an oxidative metabolic shift in Caco-2 colorectal cancer cells
Borús, D. L., Zadra, G., Minsky, D. M., Costa, M. L., Córsico, B., Storch, J., & Scaglia, N. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2025.159661 · Cited by 2
Hydrogenous and deuterated phospholipid extracts from E s c h e r i c h i a c o l i as biomimetic cytoplasmic bacterial membranes
Corucci, G., Sánchez-Puga, P., Batchu, K. C., et al. (2025) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2025.105515 · Cited by 1
Mitigating Night Biomass Loss in Outdoor Pilot‐Scale Mixotrophic Algal Cultivation of Monoraphidium minutum Using Flue Gas Condensate and Cheese Whey
Nham, Q., Gordon, T., Farnelid, H., et al. (2025) · Biotechnology and Bioengineering · doi:10.1002/bit.70027 · Cited by 1
Effect of Different Drying Methods on Biochemical Composition of Chlorella vulgaris, Microcystis aeruginosa and Haematococcus pluvialis
İnan, B. (2025) · Journal of Anatolian Environmental and Animal Sciences · doi:10.35229/jaes.1657943 · Cited by 1
Isolation and Screening of Indigenous Microalgal Strains from Diverse Ecological Niches for Lipid and Pigment Production Relevant to Sustainable Biorefinery Applications
Vijay, A. K., Prabha, S., Salim, S. A. M., Atheesh, O. S., & George, B. (2025) · BioEnergy Research · doi:10.1007/s12155-025-10869-1 · Cited by 1
“Total lipid content was estimated gravimetrically by following the modified Bligh dyer method [34] described in Vijay et al. [27] and expressed as a percentage of dry cell weight.”
Microalgae-associated Stenotrophomonas maltophilia enhances lutein production and biostimulant activity in Monoraphidium sp.
Manoel, J. A. C., Štěrbová, K., Saini, M., Bárcenas-Pérez, D., Cheel, J., Grivalský, T., Lakatos, G., Lukeš, M., Urajová, P., Ferreira, A., et al. (2025) · Algal Research · doi:10.1016/j.algal.2025.104250 · Cited by 1
Identification of Slime Mold Metabolites That Confer Protection to Commercial Crops against Root-Knot Nematodes
Hayashi, K. Y., Nagamatsu, Y., Kawano, M., et al. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c04345 · Cited by 1
A cryptic START domain regulates deeply conserved transcription factors
Dresden, C. E., Andrianova, E. P., Smith, B. J., Callery, N. I., Kolonay, D., Holub, A. S., Camacho, R. A. U., Choudury, S. G., Higgins, I. J., Zhulin, I. B., et al. (2026) · The Plant Cell · doi:10.1093/plcell/koag267 · Cited by 1
“Total lipids from 15 g of 12-d-old Arabidopsis seedling tissues were extracted using the Bligh-Dyer extraction method (Bligh and Dyer 1959).”
Investigation of the relationship between meat fatty acids and the expression levels of genes associated with lipid metabolism in Kivircik and Hungarian Merino sheep
Daldaban, F., Yalçıntan, H., Keçici, P. D., et al. (2025) · Animal Production Science · doi:10.1071/an25020 · Cited by 1
Environmental Stressors Modulating Seasonal and Daily Carbon Dioxide Assimilation and Productivity in Lessonia spicata
Troncoso, M., Fleming, Z. L., Figueroa, F. L., et al. (2025) · Plants · doi:10.3390/plants14152341 · Cited by 1
“TL was extracted from 0.05 g of freeze-dried L. spicata using chloroform/methanol (2:1, v / v ) according to the work of Bligh and Dyer [54].”
Influential
Optimised preparation of fish tissue samples for the determination of fatty acids by gas chromatography-mass spectrometry
Schöning, V., Irmler, J. R., Shaw, C., & Knopf, K. (2025) · Bulletin of the European Association of Fish Pathologists · doi:10.48045/001c.142606 · Cited by 2
Neurobehavioral and metabolic effects of prenatal low-dose chlorpyrifos in C57BL/6J mice
Kendricks, D. R., Stallone, J., Thomas, D. S., Wilson, L. R., Kirkwood‐Donelson, K. I., Jarmusch, A. K., McPherson, C. A., & Cushman, J. D. (2025) · NeuroToxicology · doi:10.1016/j.neuro.2025.08.001 · Cited by 1
Impact of Elovl5 Deficiency on Cerebellar Excitatory Synaptic Transmission in Mice
Montarolo, F., Gao, F., Loddo, M., et al. (2025) · Journal of Neuroscience · doi:10.1523/jneurosci.1529-24.2025 · Cited by 1
Native putA Overexpression in Synechocystis sp. PCC 6803 Significantly Enhances Polyhydroxybutyrate Production, Further Augmented by the adc1 Knockout Under Prolonged Nitrogen Deprivation
Utharn, S., Lindblad, P. C., & Jantaro, S. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26167815 · Cited by 1
“A dried lipid extract was obtained for analysis by evaporating the mixed chloroform fractions in a fume hood at room temperature (modified from [10,49]).”
Synthesis and Properties of Degradable Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] Derived from Waste Fish Oil
Volova, T. G., Kiselev, E. G., Sukovatyi, A. G., Zhila, N. O., Sapozhnikova, K. Y., Ipatova, N. D., & Shishatskii, P. O. (2025) · Polymers · doi:10.3390/polym17162171 · Cited by 1
“The lipid content was determined gravimetrically with chloro-form extraction [77].”
Evaluation of anti-inflammatory properties of neutral lipids, glycolipids, and phospholipids from Oncorhynchus mykiss eggs in LPS-stimulated macrophages
Rod–in, W., Lee, H. J., Lee, S.-M., Jung, S. K., & Park, W. J. (2025) · Journal of Functional Foods · doi:10.1016/j.jff.2025.106975 · Cited by 1
Comparative Metabolomics Reveals Phosphine-Induced Metabolic Disruptions in Planococcus citri (Risso)
Lee, J., Suh, S., Kim, B.-S., et al. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26168020 · Cited by 1
Effects of rainfall-induced torrential freshwater injections on river and lagoon sediments biogeochemistry
Ennas, C., Puig, P., Palanqués, A., et al. (2025) · Hydrobiologia · doi:10.1007/s10750-025-05981-4 · Cited by 1
“Lipids, after extraction in chloroform: methanol (1:1, vol: vol) (Bligh & Dyer, 1959), and evaporation in a dry hot bath at 100 °C for 20 min, were determined after the sulfuric acid carbonization procedure (Marsh & Weinstein, 1966) and expressed as tripalmitin equivalents.”
Respostas morfológicas e bioquímicas da microalga Picocystis salinarum (Chlorophyta) crescendo em regimes de depleção de nitrogênio no meio de cultura Johnson
Delgado, R. T., Guarieiro, M. D. S., & Fernandes, V. D. O. (2025) · Hoehnea · doi:10.1590/2236-8906e512022 · Cited by 1
Maturity related metabolomic analysis of Balanites aegyptiaca fruits with in vitro and in silico cytotoxicity evaluation
Abdelsalam, A., Mahran, E., Mohamed, E. T., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-15234-y · Cited by 1
“Twenty mg of each replicate was extracted using methanol, chloroform, and water in the proportions of 2:2:1.8 ( V/V/V ) based on the dry mass and water loss ratio 19,20 .”
Influential
Temporal response of belowground and aboveground organisms to microclimate changes in irrigated mediterranean vineyards
Melloul, E., Nicolas, M., Rocher, L., Bischoff, A., Gros, R., & Blight, O. (2025) · Scientific Reports · doi:10.1038/s41598-025-06257-6 · Cited by 1
“Lipids were extracted following a standard method 66 .”
Optimizing photoluminescence in carbon dots-Haematococcus pluvialis algae to increase photosynthesis and bioactive compounds
Zahri, F., Salehi-Lisar, S. Y., Razeghi, J., & Zahri, S. (2025) · Environmental Science and Pollution Research · doi:10.1007/s11356-025-36911-5 · Cited by 2
“The Bligh and Dyer method was utilized to ascertain the lipid content of H. pluvialis (Bligh and Dyer 1959).”
Unveiling the Biological Potential of Indigenous Oscillatoria spp. From Freshwater and Marine Ecosystems Through Advanced Characterization
Nayeem, J., Dey, P., Dey, S. K., & Khatoon, H. (2025) · Food Science & Nutrition · doi:10.1002/fsn3.70868 · Cited by 1
Rheology and prebiotic activity of Ora-pro-Nobis for the development of functional ingredients by 3D food printing
Sviech, F., da Silva, M. F., Goldbeck, R., et al. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107519 · Cited by 1
Study on the adjuvant activity and mechanism of action of a novel Monophosphoryl Lipid A
Zhao, L., Shi, P., Zhang, Y., et al. (2025) · Molecular Immunology · doi:10.1016/j.molimm.2025.08.019 · Cited by 1
Membrane adaptation of Ochrobactrum intermedium under salt stress enables growth promotion in peanut plants
Reguera, Y. B., Cesari, A. B., Biasutti, M. A., et al. (2025) · Journal of Applied Microbiology · doi:10.1093/jambio/lxaf227 · Cited by 1
Biomonitoring of polycyclic aromatic hydrocarbons (PAHs) distribution in wetlands: A case study using Alternanthera ficoidea (L.) plant in West Bengal, India
Dhara, A., & Dutta, R. (2025) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2025.118663 · Cited by 1
Valorization of agro-industrial by-products via aroma-rich fermentation with amazonian and food-grade fungi
Martins, G. A., Rocha, L. D. O., Maróstica, M. R., Sales-Campos, C., Chevreuil, L. R., de Jesus, M. A., & Bicas, J. L. (2025) · Applied Food Research · doi:10.1016/j.afres.2025.101330 · Cited by 2
The impact of the unharvested reeds on the nitrogen and phosphorus cycles in Lake Dongting
Li, H., Deng, H., Li, J., et al. (2025) · Journal of Freshwater Ecology · doi:10.1080/02705060.2025.2557554 · Cited by 1
“Lipid (LIP) was determined according to Bligh and Dyer (1959) and Marsh and Weinstein (1966).”
Leaf Nitrogen Spraying Improves Photosynthesis, Seed Quality and Yield of Soybean Plants Facing post-flowering Phosphorus Deficiency
Laira, M. D., Martins, T. D. S., Almeida, R. L., et al. (2025) · Journal of soil science and plant nutrition · doi:10.1007/s42729-025-02707-2 · Cited by 1
“The seed oil content was determined according to the method described by Blich and Dyer ( 1959).”
Warming reduces soil priming effects in a subtropical forest regardless of soil depth and carbon input amount
Bai, X., Zhang, Q., Feng, J., Wu, X., Zeng, Q., Zhang, X., Yuan, X., Ni, X., & Chen, Y. (2025) · Plant and Soil · doi:10.1007/s11104-025-07849-x · Cited by 1
“Soil microbial community composition was characterized using phospholipid fatty acid (PLFA) analysis following the methodology of Bligh and Dyer (1959).”
Development of plant extracts as substrates for untargeted transporter substrate identification in Xenopus oocytes
Theodorou, C., de-Prado-Parralejo, V., Xu, D., et al. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1640426 · Cited by 1
Improving microalgae harvesting efficiency: electrochemical techniques and parameter optimization
Yakamercan, E., Guleria, S., Karimi, M., Aygün, A., Bhasin, A., & Şimşek, H. (2025) · Environmental Science Water Research & Technology · doi:10.1039/d5ew00518c · Cited by 1
Description of Neorhizobium tunisiense sp. nov., reclassification of Neorhizobium xiangyangii as Pseudorhizobium xiangyangii comb. nov. and proposal of Terrirhizobium terrae gen. nov., comb. nov. to accommodate the divergent species Aliirhizobium terrae
Sbissi, I., Boussoufa, D., Kammoun, I., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006912 · Cited by 1
Effect of the Partial Replacement of Wheat Flour by Soft or Hard Jackfruit (A rtocarpus Heterophyllus Lam) Seeds Flour on the Composition of Cupcakes
Hajj, V. F., Fonseca, G. G., Lopes, A. P., Visentainer, J. V., & Petenuci, M. E. (2025) · Journal of Culinary Science & Technology · doi:10.1080/15428052.2025.2563853 · Cited by 1
“The lipid content was obtained by Bligh and Dyer (1959) method.”
Comparative Analysis of Physicochemical Properties and Bioactive Compounds in Two Hilly Region Pineapple Varieties (Ananas comosus L.) for Value-Added Food Applications
Akbar, G. W., Hojaifa, U., Tania, I., Bristy, N. N., Chowdhury, T., & Absar, N. (2025) · Journal of Plant Science and Phytopathology · doi:10.29328/journal.jpsp.1001157 · Cited by 1
Trophic ecology of Ophiuroidea and Asteroidea in the Clarion-Clipperton-Fracture Zone (Central Pacific)
Stratmann, T., Simon‐Lledó, E., van der Meer, M. T. J., et al. (2025) · Deep Sea Research Part I Oceanographic Research Papers · doi:10.1016/j.dsr.2025.104598 · Cited by 1
Valorization of Desmococcus olivaceus algal biomass and biochemicals in raceway pond phycoremediation of electroplating chrome sludge
Muthukumaran, M. (2025) · Bioresource Technology Reports · doi:10.1016/j.biteb.2025.102305 · Cited by 1
Microparticles of buriti oleosomes produced with different wall materials
Melo, F. D. S., Barroso, L. A., Galvão, A. M. M. T., et al. (2025) · Food Research International · doi:10.1016/j.foodres.2025.117516 · Cited by 1
Enhancing chlorella minutissima biofuel and bioproduct potential: chelated trace elements and sustainable metal recovery
Saini, M., & Nazurally, N. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04555-9 · Cited by 1
“Lipid extraction was performed according to the method of Bligh and Dyer (1959): 1 g of lyophilized bio - mass was mixed with a chloroform: methanol solution (2:1, v/v), sonicated for 15 min, and subjected to phase separation using 0.9% NaCl.”
Influential
Further evidence supporting the existence and accumulation of triacylglycerol and acylplastoquinol in cyanobacteria
Sato, N., Jimbo, H., Yoshitomi, T., Shoji, T., Sato, M., Takeda‐Kamiya, N., Toyooka, K., & Wada, H. (2025) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiaf478 · Cited by 1
“Total lipids were extracted from the harvested cells by the Bligh and Dyer (1959) method.”
The association between fatty acid composition and hypertension and the effects of pharmacotherapeutic approaches: a cross-sectional study
Ayhan, İ., Çam, S. A., Uysal, F., Ayhan, H., Arslan, S. O., & Omar, I. A. (2025) · Pamukkale Medical Journal · doi:10.31362/patd.1707477 · Cited by 1
Functional characterization of lactate metabolism and its key regulatory genes reveals lactate-mediated modulation of carbon and nitrogen metabolism in Phaeodactylum tricornutum
Duan, J., Zhang, Y., Li, C., et al. (2025) · Microbial Cell Factories · doi:10.1186/s12934-025-02779-w · Cited by 1
“After centrifugation and collection, the cells were flash-frozen in liquid nitrogen and freeze-dried, and total lipids were extracted as described previously with minor modifications [19].”
Tailoring mixed microbial consortia for sustainable biodiesel: An attempt towards synergistic biomass production and cost-effective harvesting in pilot scale open cultivation
Saravanabhupathy, S., Karmakar, S., & Banerjee, R. (2025) · Sustainable Chemistry and Pharmacy · doi:10.1016/j.scp.2025.102228 · Cited by 1
Probiotic Fermentation Combined with Dual Enzyme Hydrolysis Improves the Flavor and Processing Characteristics of Sea Cucumber Peptides
Wu, S., Han, G., Chen, G., et al. (2025) · Food Biophysics · doi:10.1007/s11483-025-10025-4 · Cited by 1
“Protein content was determined using the Kjeldahl method [29]; Lipid content was determined gravimetrically after extraction as described by Bligh and Dyer [30]; Determination of total sugars with reference to the method of Li Ying [31].”
Metabolic Effects of Dietary Chlorella vulgaris Evaluated by Multiorgan Metabolomics in Weaning Piglets
Pedro, M. F., Matzapetákis, M., Maia, M. R. G., Fonseca, A. J. M., Cabrita, A. R. J., Alves, S. P., Freire, J. P. B., Bessa, R. J. B., Zervoú, M., & de Almeida, A. M. (2025) · ACS Agricultural Science & Technology · doi:10.1021/acsagscitech.5c00646 · Cited by 1
Calcium oxalate alters lipid profiles and annexin A2 enrichment in membrane fractions of murine inner medullary collecting duct cells
Shahzad, Z., Rafay, R. H., Khan, S. R., et al. (2025) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2025.130868 · Cited by 1
Polyketide synthase–like functionality acquired by plant fatty acid elongase
Kim, H., Park, K., Jia, H., et al. (2026) · Science Advances · doi:10.1126/sciadv.aed2892 · Cited by 1
“Total lipids were extracted from ~ 20 mg of Orychophragmus seeds, soybean seeds, and Arabidopsis seeds by a modified version of the Bligh-Dyer method ( 39 , 40 ).”
Designing biologically-relevant cell membrane models with natural lipid mixtures
Batchu, K. C., Corucci, G., Laux, V., Gabel, F., Fragneto, G., & Luchini, A. (2025) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2025.184474 · Cited by 1
The PA2803-encoded PcrP exhibits a novel non-catalytic function and contributes to polymyxin B resistance in P. aeruginosa
Salpadoru, T., Khanam, S., Borin, V. A., et al. (2025) · Journal of Bacteriology · doi:10.1128/jb.00189-25 · Cited by 1
Plant Lipid Droplets and Derived Lipidic Nano‐Assemblies: Structure, Biogenesis and Pharmaceutical Applications
Hanano, A., Yousfan, A., & Murphy, D. J. (2025) · Plant Biotechnology Journal · doi:10.1111/pbi.70411 · Cited by 1
Assessment of foam drying methods on the retention and characterization of bioactive compounds in Bacaba (Oenocarpus bacaba) powder
de Cól, C. D., Utpott, M., Rios, A. D. O., et al. (2025) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-025-03719-3 · Cited by 1
Distribution and sources of organic matter in submarine canyons incising the Gulf of Palermo, Sicily: A multi-parameter investigation
Paradis, S., Gies, H., Moccia, D., et al. (2025) · Biogeosciences · doi:10.5194/bg-22-5921-2025 · Cited by 1
Pomegranate peel supplementation improves performance and lipid metabolism in laying hens
Bölükbaşı, Ş. C., & Ürüşan, H. (2025) · Archives animal breeding/Archiv für Tierzucht · doi:10.5194/aab-68-629-2025 · Cited by 1
“…24 h, and the dry weights were determined The ratio of wet to dry liver weight was calculated To quantify the total lipid content in the liver, the Bligh and Dyer (1959) method was employed Approximately 1 g of homogenized liver tissue was mixed with a solvent mixture of chloro-form, methanol,…”
Effect of Fractionation Technology on Nutrient Profile of Protein-Rich Ingredients from Hollow Brown Crab (Cancer pagurus)
Abdollahi, M., Pezeshk, S., & Undeland, I. (2025) · Food and Bioprocess Technology · doi:10.1007/s11947-025-04058-3 · Cited by 1
Alternative solvent systems for Yarrowia lipolytica oil extraction: cell disruption, lipid recovery and oil properties
Machado, B. R., Lopes, H. S. P., Sala, L., Duarte, S. H., & Santos, L. O. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04652-9 · Cited by 1
“Lipids were extracted from pretreated biomass, according to Bligh and Dyer ( 1959 ).”
Sewage to energy: harnessing algae-fungi consortium for bioremediation
Yadav, D. K., Patel, A., & Kumar, R. (2025) · Energy Ecology and Environment · doi:10.1007/s40974-025-00395-3 · Cited by 1
Statistical optimization and scaled-up bioreactor fermentation for enhanced edible oil production from palm date waste by Rhodotorula glutinis KAEC-61
Elazzazy, A. M., Baeshen, M. N., Alasmi, K. M., Alqurashi, S. I., Hafiz, H., Saleh, T. S., Haroun, M. Y., Khattab, S. M. R., & Desouky, S. E. (2025) · Scientific Reports · doi:10.1038/s41598-025-22643-6 · Cited by 3
“The supernatant was discarded, and the cell pellet was used for gravimetric lipid quantification and FAME preparation 34 .”
The first report on the dorso-ventral nutritional variations of high value marine finfish, the humphead wrasse (Cheilinus undulatus)
Nugraha, R., Shapawi, R., Mazlan, N. B., et al. (2025) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2025.108563 · Cited by 1
Bridging the gaps: advanced techniques to unlock lipid function in plant reproductive development
Guo, Z., & Chye, M. (2025) · Plant Reproduction · doi:10.1007/s00497-025-00532-2 · Cited by 1
“The Folch method (Folch et al. 1957) and Bligh and Dyer method (Bligh and Dyer 1959) are classical techniques for lipid extraction, with the former predominantly used for solid tissue and the latter more effective for biological fluids (Pati et al. 2016).”
Associations of urinary environmental phenols with obesity and lipid metabolism in children and adolescents: The expanded Chinese National Human Biomonitoring (CNHBM) project
Jiang, N., Chen, Q., Cheng, B.-J., Ke, M., Wang, J., Li, T., Chen, H., Liao, Y., Sun, H. W., & Liu, R. (2025) · Environmental Research · doi:10.1016/j.envres.2025.123297 · Cited by 2
Suplementation of the microalgae Arthrospira platensis powder in the nursery phase of Pacific white shrimp (Penaeus vannamei, Boone 1931) in a biofloc system
Rungo, N., Carvalho, A., Ribeiro, V., et al. (2025) · Aquaculture International · doi:10.1007/s10499-025-02302-3 · Cited by 1
“Total lipids were determined using the cold extraction method with chloroform and methanol, according to the methodology of Bligh and Dyer (1959).”
Tenacibaculum salmonis sp. nov., isolated from Atlantic salmon (Salmo salar L.) fish farmed in Chile
Avendaño-Herrera, R. E., Irgang, R., & Lopez, P. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006963 · Cited by 1
“After harvesting, cells were suspended in an isopropanol/water solution (1:1, v/v) and analysed according to Bligh and Dyer [36] and Tindall et al .”
Indigenous method of microalgal biomass conversion into biodiesel, carotenoids and pharmacological agent
Josephine, A., Kumar, T. S., Sekar, A. K., Rajakumar, S., Kirubagaran, R., & Dharani, G. (2025) · Chemical Papers · doi:10.1007/s11696-025-04466-x · Cited by 1
Ferroptosis in Tubular Epithelial Cells Across Distinct Renal Regions Is a Primary Causal Factor for Lupus Nephritis
Jin, W., Chen, H., Zhang, S., Xu, X., Zhao, P., Hu, X., Xie, G., Du, Y., Wen, C., Lu, L., et al. (2025) · Antioxidants and Redox Signaling · doi:10.1177/15230864251396446 · Cited by 2
“As previously described (Bligh and Dyer, 1959), lipids were extracted from renal samples using a modi ﬁ ed Bligh and Dyer method, incorporating an internal standard.”
Optimizing multiomics sample preparation: comparative evaluation of extraction protocols for HepG2 cells
Arnst, T. F., Hemmer, S., Fecher‐Trost, C., Wagmann, L., & Meyer, M. R. (2025) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-025-06235-x · Cited by 2
“The suitability of the MTBE-based procedure for sole lipidomics analysis was thoroughly described in literature, since it was initially developed as an alternative for established extractions for comprehensive lipid profiling [21, 60–62].”
Standing durations of dead emergent litters influence their underwater decomposition and carbon sequestration in wetlands
Ji, D., Zhou, J., Xie, D., Zhang, X., Wu, F., Yang, J., Gao, J., Deng, J., Wang, Q.-W., Xu, X., et al. (2025) · Plant and Soil · doi:10.1007/s11104-025-08099-7 · Cited by 1
“The detailed procedures for PLFA analysis in this study adhered to a modified method described by Dippold (Dippold and Kuzyakov 2016) and Bligh (Bligh and Dyer 1959).”
Foliar Application of Seaweed-based Biostimulants Enhances Drought Resilience, Physiological Performance, and Seed Oil Quality in Sesame (Sesamum indicum L.)
Alayafi, A. A. M. (2025) · Journal of soil science and plant nutrition · doi:10.1007/s42729-025-02810-4 · Cited by 1
“Oil content was expressed as a percentage of dry seed weight, calculated as (lipid mass/dry seed mass) × 100 (Bligh and Dyer 1959).”
Grumixama extract as natural additive on fermented sausage to control lipid oxidation
Ojoli, G. X., Mangueira, R. A. F., Alves, C. A., Bertuci, M. L., & Barretto, A. C. D. S. (2025) · Meat Science · doi:10.1016/j.meatsci.2025.109999 · Cited by 1
Loss of LafB activity reverses daptomycin resistance in E. faecium
de Mello, S. S., Schultz, B. J., Goh, B., Grote, A., Shea, T., Muscato, J. D., Oh, S. F., Manson, A. L., Earl, A. M., Walker, S., et al. (2025) · mBio · doi:10.1128/mbio.00715-25 · Cited by 2
“A modified Bligh-Dyer method (46, 47) was used to extract total lipids from the bacteria.”
Unveiling the Interplay: Salinity‐Modulated Defence Mechanisms in Medicago truncatula Against Phoma medicaginis Infection
Chaouachi, M., Nouairi, I., Khiari, B., Badri, M., Rickauer, M., Gentzbittel, L., Ben, C., & Djébali, N. (2025) · Plant Pathology · doi:10.1111/ppa.70097 · Cited by 1
Hydroethanolic Soy Extract Rich in Isoflavones Protects Dystrophic Mutant Mice From Oxidative Stress and Inflammation via the ROS / NF ‐ κB Feedback Pathway
Nascimento, B. G., Ferreira, L. L., Corsini, W., da Silva, M. D., Teodoro, F. P., Santos, A. G. M., Macedo, W. D. M. F., Rostagno, M. A., Silva, M. J. D., de Souza, C. V. E., et al. (2025) · BioFactors · doi:10.1002/biof.70062 · Cited by 1
“Therefore, to remove the lipids, a maceration process with hexane was used for 24 h, adapting the method of Bligh and Dyer (1959) [20].”
Synergistic effect of photonic modulation and nutrient deprivation on lipid accumulation in Scenedesmus sp. GUEco1020: A potent microalga for biodiesel production
Bhattacharjee, S., & Baruah, P. P. (2025) · Chemical Engineering Journal Green and Sustainable · doi:10.1016/j.cejgas.2025.100012 · Cited by 1
Description of three new Dryocola species: Dryocola mayonis sp. nov., Dryocola sharpae sp. nov.and Dryocola baronae sp. nov., isolated from human clinical samples
Mohammed, H. T., Koscianski, C. A., Gordy, G. G., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006986 · Cited by 1
“…were extracted from freeze-dried cell material of strains BD586 T , BD613 T , BD626 T and control strain, D. boscaweniae CCUG 76178, with a chloroform:methanol:0.3% aqueous NaCl mixture, with recovery into the chloroform phase (modified from [25], and separated by two-dimensional silica gel TLC.”
Influential
Synergistic co-culture of Desertifilum tharense MASKD1 and bacterial consortium DBPS1 in an integrated biorefinery for sewage remediation and recovery of phycocyanin, biodiesel and green hydrogen
Andhare, P., Dube, S., Sonavane, B., Pataniya, P. M., Sumesh, C. K., Desai, C., Kikani, B. A., & Madamwar, D. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04696-x · Cited by 1
“A modified Bligh and Dyer method was performed to deter - mine total lipids (Bligh and Dyer 1959).”
Evaluating Salinity Stress-Induced Changes in Chlorella vulgaris: Assessing its Suitability for Nutritional and Biodiesel Applications
Gürsoy, A. N., Güngör, Z., Özdemir, T., Çam, A. O., & Akçaalan, R. (2025) · Waste and Biomass Valorization · doi:10.1007/s12649-025-03420-y · Cited by 2
“Modified Bligh and Dyer (1959) method was used to deter - mine the lipid content [41 ].”
Wheat Germ Oil for Enhanced Stability of Microencapsulated Fish Oil During Spray Drying and Optimization of Coating Component Ratios
Özyurt, G., Daday, M. T., Sakarya, Y., et al. (2025) · Food and Bioprocess Technology · doi:10.1007/s11947-025-04142-8 · Cited by 1
The structure-dependent effects of newly synthesised cationic gemini surfactants against yeast cells
Mazurkiewicz, E., Obłąk, E., Paściak, M., Gamian, A., Ciesielska, M., Lamch, Ł., & Wilk, K. A. (2025) · Scientific Reports · doi:10.1038/s41598-025-30210-2 · Cited by 2
“Lipid extraction was conducted using the Bligh-Dyer method 98 .”
Development and characterization of bioplastics based on different varieties of corn starch (Zea mays L.): Morphological, thermal and mechanical properties
Ferraz, M., Nogueira, G. F., Buontempo, R. C., et al. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.149550 · Cited by 1
Sphingobium salicis sp. nov., a novel endophyte with two subspecies from pioneer plants growing on primary substrates
Tournay, R. J., Freeman, J. L., Levish, M., Baker, D., Firrincieli, A., & Doty, S. L. (2025) · Antonie van Leeuwenhoek · doi:10.1007/s10482-025-02214-5 · Cited by 2
“Polar lipids were extracted according to Bligh and Dyer (1959) and separated by two-dimensional thin-layer chromatography as described by Tindall et al. (2010), with lipid spots visualized using molybdophosphoric acid and group-specific spray reagents.”
Exploring the feeding ecology of 16 mesopelagic fish species using fatty acid trophic markers
Zhang, B., Pethybridge, H., Virtue, P., Chang, K. J. L., & Nichols, P. D. (2025) · Marine Biology · doi:10.1007/s00227-025-04769-3 · Cited by 2
“Subsamples (around 0.15 g dry weight) of whole fish samples were extracted overnight following the modified Bligh and Dyer ( 1959 ) method, using a solvent mixture of methanol: dichloromethane: Milli-Q water (MeOH: CH 2 Cl 2 :H 2 O) in the ratio 30:15:12 mL. Phase separation was achieved the…”
Influential
Evaluating compost tea recipes for enhanced nutrient cycling: An innovative management practice for sustainable soil health
Saad, A., Shah, S. H. H., & Carpenter-Boggs, L. (2025) · Applied Soil Ecology · doi:10.1016/j.apsoil.2025.106618 · Cited by 1
Modulatory effects of light intensity and temperature on fatty acid profiles in the diatom Sellaphora minima: Implications for aquaculture and biofuel applications
Krishnan, A., & Joseph, A. (2025) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2025.108792 · Cited by 1
Relations of the O-horizon with canopy tree species and hydropedologic soil types
Studer, E. A., Bailey, S. W., Huene, B. L. V. H., et al. (2025) · Biogeochemistry · doi:10.1007/s10533-025-01290-z · Cited by 1
“PLFA extraction followed Bligh and Dyer (1959).”
Prediction and characterization of lipid-interacting proteins
Alfonso, S., Decosto, C. M., Chatterjee, P., Precord, T., & Dassama, L. M. K. (2025) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2025.11.013 · Cited by 1
Synergistic pollutant removal and high-value biomass production via Galdieria sulphuraria-based starch wastewater treatment
Guo, Z., Liu, W., Chen, B., Yin, Y., Zhang, X., Lin, J., Wang, L., & Wang, Z. (2025) · Journal of Environmental Management · doi:10.1016/j.jenvman.2025.128405 · Cited by 2
A Polymeric Nanocarrier Platform for Rapid and Precise Fatty Acid Tracing in Cells
Vincent, M. P., Ellis, A. E., DeCamp, L. M., Kitchen-Goosen, S. M., Rogers, T. J., Tarach, A. R., Isaguirre, C., Compton, S. E., Lien, E. C., Williams, K. S., et al. (2025) · ACS Applied Bio Materials · doi:10.1021/acsabm.5c01770 · Cited by 1
Feasibility Study of Soybean Oil-Fortified Foods to Alter Blood Content of Linoleic Acid and Body Weight: A Randomized Double-Masked Placebo-Controlled Crossover Trial
Cole, R. M., Colombo, E., Angelotti, A., Sparagna, G. C., Choriego, R. E., Jiménez-Flores, R., Ni, A., & Belury, M. A. (2025) · Journal of Nutrition · doi:10.1016/j.tjnut.2025.101288 · Cited by 1
“In brief, PBMC lipids were isolated using a modified Bligh and Dyer method [52], using 1 μ m tetramyristal CL as an internal standard (Avanti Polar Lipids).”
Characterization of age-related dolichol increases in the retina of the C57BL/6 mouse
Guan, O. Y., Li, Y., Guan, Z., & Wen, R. (2025) · Scientific Reports · doi:10.1038/s41598-025-28509-1 · Cited by 1
“Total lipids were extracted from retinal samples using a modified Bligh and Dyer method 41 as described previously 5,42 .”
Sideways lipid presentation by the antigen-presenting molecule CD1c
Cao, T.-P., Liao, G., Cheng, T., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-67732-2 · Cited by 1
“Molecular replacement was performed using Phaser-MR 49 with the high-resolution structure of CD1c-MPM1 (PDBID:7MX4)asthesearchmodelforCD1cdatasets,CD1a-SM 42:2(PDBID:7KP0)forCD1a-GD3dataset,andCD1b-PC(PDBID:6D64) for CD1b-GD3.”
Comparative antibacterial activity of lipid and pigment extracts from marine diatoms and dinoflagellates shows higher inhibitory effect against Gram-positive bacteria
Toledo, L., Ochoa-Álvarez, N. A., Magallón‐Servín, P., Arjona-López, O., Palacios, E., Hernández-Sandoval, F. E., Arredondo-Vega, B. O., & Estrada, N. (2026) · Next research. · doi:10.1016/j.nexres.2026.101308 · Cited by 1
What Is a Healthy Soil for Winegrape Production? Grower Perspectives Differ From Soil Health Paradigms
Gonzalez‐Maldonado, N., Steenwerth, K. L., Nocco, M. A., et al. (2026) · European Journal of Soil Science · doi:10.1111/ejss.70265 · Cited by 1
The food grade bacterium Lactobacillus helveticus VEL12193 promotes autophagy by releasing membrane vesicles
Bringer, M.-A., Manceau, S., Azzaz, J. A., Buteau, B., Proukhnitzky, L., Aumeunier, A., Bermúdez‐Humarán, L. G., Chain, F., Daniel, C., Jacquin, É., et al. (2026) · Cell Communication and Signaling · doi:10.1186/s12964-025-02616-y · Cited by 1
“Total lipids were extracted with the acidified Bligh & Dyer method [64].”

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks