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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Abstract
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.
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“in addition to gravimetry measurements [14,15], a large panel of chromatography strategies have been developed, like thin-layer chromatography (TLC), high-pressure liquid chromatography (HPLC) and gas chromatography (GC) coupled with mass spectrometry (MS) [16].”
From The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae (2015) · cites this paper as [14]
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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citationBonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18
Research works printed but not linked: 1
- BANCROFT W., 1895, Phys. Iiev., 3, 1
Cited by
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Items 47,301–47,400 of 48,847
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Orekhov, A. N., Tertov, V. V., Pivovarova, E. M., et al. (1990) · Springer eBooks · doi:10.1007/978-94-009-0731-7_46
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Reinelt, L. (2021) · Lancaster EPrints (Lancaster University) · doi:10.17635/lancaster/thesis/1433
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Viner, K. (2017) · QSpace (Queen's University Library)
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Gurgone, F. (2011) · Clío: Revista de historia
[Legal compendium on women's rights and the Chilean child regarding breast feeding].
Scroggie, S. (1981) · PubMed
Elthon, T. E., & McIntosh, L. (1987) · Springer eBooks · doi:10.1007/978-1-4899-3517-5_19
The Effect of Antagonists to PAF Activity, Vascular Permeability Increase.
真理子, 山. (1992) · The Journal of Kansai Medical University · doi:10.5361/jkmu1956.44.1_9
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Kimiko, M. U. T. O., Kurosaka, M., Masumi, U. S. U. I., et al. (1981) · The Journal of Japan Atherosclerosis Society · doi:10.5551/jat1973.9.1_49
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Wright, E. A. (2013) · OhioLink ETD Center (Ohio Library and Information Network)
Orevi, M., Eldor, A., Gödeke, M.-E., et al. (1996) · Advances in experimental medicine and biology · doi:10.1007/978-1-4899-0179-8_13
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SEKIMOTO, H., Shimada, O., Uemura, E., et al. (1982) · The Journal of Japan Atherosclerosis Society · doi:10.5551/jat1973.9.6_887
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Khamankar, D. B., Kamdi, R. R., & Sawane, A. P. (2012) · Environment Conservation Journal · doi:10.36953/ecj.2012.131230
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Balkenhohl, T. J. (2000) · Digitalen Hochschulbibliothek Sachsen-Anhalt (Universitäts- und Landesbibliothek Sachsen-Anhalt) · doi:10.25673/2621
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Grossert, J. S., & Swee, T. (1977) · Springer eBooks · doi:10.1007/978-1-4684-0802-7_10
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Richard, V. R. (2014) · Spectrum Research Repository (Concordia University)
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Sakai, M., MAKINO, T., WATANABE, M., et al. (1983) · The Journal of Japan Atherosclerosis Society · doi:10.5551/jat1973.11.4_911
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Schmitt, C. S., Hewitt, P., Kabiri, M., & Amberg, A. (2013) · Springer eBooks · doi:10.1007/978-3-642-25240-2_61
Borowitzka, M. A. (2012) · CRC Press eBooks · doi:10.1201/b11711-30
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Metadata
- Authors
- Bligh, E. G., & Dyer, W. J.
- Venue
- Canadian Journal of Biochemistry and Physiology
- Publisher
- Canadian Science Publishing
- Published
- 1959-08-01
- Volume
- 37
- Issue
- 8
- Pages
- 911-917
- ISSN
- 0576-5544 (ISSN-L)
- OpenAlex
- W2152311269
- Cited by
- 54,751
- References
- 5
- Citations by year
- 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
- FWCI
- 8.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Biochemistry and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Keywords
- lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
- MeSH terms
- Fishes, Lipids
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