Principles and Biophysical Applications of Lanthanide-Based Probes
Abstract
▪ Abstract Using luminescent lanthanides, instead of conventional fluorophores, as donor molecules in resonance energy transfer measurements offers many technical advantages and opens up a wide range of new applications. Advantages include farther measurable distances (∼100 Å) with greater accuracy, insensitivity to incomplete labeling, and the ability to use generic relatively large labels, when necessary. Applications highlighted include the study of ion channels in living cells, protein-protein interaction in cells, DNA-protein complexes, and high-throughput screening assays to measure peptide dimerization associated with DNA transcription factors and ligand-receptor interactions.
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Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,948 · see citing works
Tsien, R. Y. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.509 · Cited by 6,178 · see citing works
Energy transfer: a spectroscopic ruler.
Stryer, L., & Haugland, R. P. (1967) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.58.2.719 · Cited by 1,961 · see citing works
Spragg, S. P. (1979) · Nature · doi:10.1038/278085a0 · Cited by 1,707 · see citing works
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
Griffin, B. A., Adams, S., & Tsien, R. Y. (1998) · Science · doi:10.1126/science.281.5374.269 · Cited by 1,553 · see citing works
The renaissance of fluorescence resonance energy transfer.
Selvin, P. R. (2000) · Nature Structural Biology · doi:10.1038/78948 · Cited by 998 · see citing works
Self-citationFluorescence resonance energy transfer
Clegg, R. M. (1995) · Current Opinion in Biotechnology · doi:10.1016/0958-1669(95)80016-6 · Cited by 932 · see citing works
Europium as a label in time-resolved immunofluorometric assays
Hemmilä, I. A., Dakubu, S., Mukkala, V.-M., Siitari, H., & Lövgren, T. N.-E. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90095-2 · Cited by 833 · see citing works
Dale, R. E., Eisinger, J., & Blumberg, W. E. (1979) · Biophysical Journal · doi:10.1016/s0006-3495(79)85243-1 · Cited by 760 · see citing works
Rhodamine B and rhodamine 101 as reference substances for fluorescence quantum yield measurements
Karstens, T., & Kobs, K. (1980) · The Journal of Physical Chemistry · doi:10.1021/j100451a030 · Cited by 680 · see citing works
Determination of the absolute quantum yield of fluorescent solutions
Weber, G., & Teale, F. W. J. (1957) · Transactions of the Faraday Society · doi:10.1039/tf9575300646 · Cited by 665 · see citing works
Direct Physical Measure of Conformational Rearrangement Underlying Potassium Channel Gating
Mannuzzu, L. M., Moronne, M. M., & Isacoff, E. Y. (1996) · Science · doi:10.1126/science.271.5246.213 · Cited by 604 · see citing works
[13] Fluorescence resonance energy transfer
Selvin, P. R. (1995) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(95)46015-2 · Cited by 521 · see citing works
Self-citationAtomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy
Cha, A., Snyder, G. E., Selvin, P. R., et al. (1999) · Nature · doi:10.1038/45552 · Cited by 474 · see citing works
Self-citationRare earth cryptates and homogeneous fluoroimmunoassays with human sera
Mathis, G. (1993) · Clinical Chemistry · doi:10.1093/clinchem/39.9.1953 · Cited by 393 · see citing works
Characterizing Voltage-Dependent Conformational Changes in the K Channel with Fluorescence
Cha, A., & Bezanilla, F. (1997) · Neuron · doi:10.1016/s0896-6273(00)80403-1 · Cited by 340 · see citing works
Mathis, G. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.9.1391 · Cited by 339 · see citing works
Luminescence energy transfer using a terbium chelate: improvements on fluorescence energy transfer.
Selvin, P. R., & Hearst, J. E. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.21.10024 · Cited by 301 · see citing works
Self-citation[17] The use of singlet-singlet energy transfer to study macromolecular assemblies
Fairclough, R. H., & Cantor, C. R. (1978) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(78)48019-x · Cited by 298 · see citing works
Xiao, M., & Selvin, P. R. (2001) · Journal of the American Chemical Society · doi:10.1021/ja0031669 · Cited by 290 · see citing works
Self-citationSpectroscopic mapping of voltage sensor movement in the Shaker potassium channel
Glauner, K. S., Mannuzzu, L. M., Gandhi, C. S., et al. (1999) · Nature · doi:10.1038/45561 · Cited by 283 · see citing works
Crystal Structure of a σ70 Subunit Fragment from E. coli RNA Polymerase
Malhotra, A., Severinova, E., & Darst, S. A. (1996) · Cell · doi:10.1016/s0092-8674(00)81329-x · Cited by 281 · see citing works
Stoichiometry of a Ligand-gated Ion Channel Determined by Fluorescence Energy Transfer
Farrar, S. J., Whiting, P. J., Bonnert, T. P., & McKernan, R. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.15.10100 · Cited by 269 · see citing works
Clegg, R. M., Murchie, A. I. H., Zechel, A., et al. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.7.2994 · Cited by 266 · see citing works
Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice
Pope, A. (1999) · Drug Discovery Today · doi:10.1016/s1359-6446(99)01340-9 · Cited by 199 · see citing works
Udenfriend, S. (1969) · Elsevier eBooks · doi:10.1016/b978-0-12-705802-3.50005-9 · Cited by 193 · see citing works
Kapanidis, A. N., Ebright, Y. W., & Ebright, R. H. (2001) · Journal of the American Chemical Society · doi:10.1021/ja017074a · Cited by 193 · see citing works
Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay
Siitari, H., Hemmilä, I. A., Soini, E., Lövgren, T. N.-E., & Koistinen, V. (1983) · Nature · doi:10.1038/301258a0 · Cited by 185 · see citing works
Diffusion-Enhanced Fluorescence Energy Transfer
Stryer, L., Thomas, D. D., & Meares, C. F. (1982) · Annual Review of Biophysics and Bioengineering · doi:10.1146/annurev.bb.11.060182.001223 · Cited by 159 · see citing works
Protein mobility and GABA-induced conformational changes in GABAA receptor pore-lining M2 segment
Horenstein, J., Wagner, D. A., Czajkowski, C., & Akabas, M. H. (2001) · Nature Neuroscience · doi:10.1038/87425 · Cited by 155 · see citing works
Synthesis of Europium(III) Chelates Suitable for Labeling of Bioactive Molecules
Takalo, H., Mukkala, V.-M., Mikola, H., Liitti, P., & Hemmilä, I. A. (1994) · Bioconjugate Chemistry · doi:10.1021/bc00027a015 · Cited by 145 · see citing works
Helmann, J. D., & deHaseth, P. L. (1999) · Biochemistry · doi:10.1021/bi990206g · Cited by 141 · see citing works
Saha, A. K., Kross, K., Kloszewski, E. D., et al. (1993) · Journal of the American Chemical Society · doi:10.1021/ja00076a088 · Cited by 140 · see citing works
Vámosi, G., Gohlke, C., & Clegg, R. M. (1996) · Biophysical Journal · doi:10.1016/s0006-3495(96)79300-1 · Cited by 139 · see citing works
Lanthanide-based resonance energy transfer
Selvin, P. R. (1996) · IEEE Journal of Selected Topics in Quantum Electronics · doi:10.1109/2944.577339 · Cited by 126 · see citing works
Self-citationModern bioassays using metal chelates as luminescent probes
Sammes, P. G., & Yahioglu, G. (1996) · Natural Product Reports · doi:10.1039/np9961300001 · Cited by 121 · see citing works
Jovin, T. M., & Arndt‐Jovin, D. J. (1989) · Elsevier eBooks · doi:10.1016/b978-0-12-417760-4.50012-4 · Cited by 115 · see citing works
Rotational movement during cyclic nucleotide-gated channel opening
Johnson, J. P., & Zagotta, W. N. (2001) · Nature · doi:10.1038/35091089 · Cited by 107 · see citing works
Monomolecular Layers and Light
Drexhage, K. H. (1970) · Scientific American · doi:10.1038/scientificamerican0370-108 · Cited by 107 · see citing works
Xu, Y. Y., Pettersson, K. S. I., Blomberg, K. R., Hemmilä, I. A., Mikola, H., & Lövgren, T. N.-E. (1992) · Clinical Chemistry · doi:10.1093/clinchem/38.10.2038 · Cited by 106 · see citing works
Core RNA Polymerase from E. coli Induces a Major Change in the Domain Arrangement of the σ70 Subunit
Callaci, S., Heyduk, E., & Heyduk, T. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80313-5 · Cited by 104 · see citing works
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
Miller, C., Blaustein, R. O., Cole, P. A., et al. (2000) · Nature Structural Biology · doi:10.1038/74076 · Cited by 100 · see citing works
Time-resolved delayed luminescence image microscopy using an europium ion chelate complex
Marriott, G., Heidecker, M., Diamandis, E. P., et al. (1994) · Biophysical Journal · doi:10.1016/s0006-3495(94)80597-1 · Cited by 92 · see citing works
Li, M., & Selvin, P. R. (1997) · Bioconjugate Chemistry · doi:10.1021/bc960085m · Cited by 84 · see citing works
Self-citationCheung, H. C. (2006) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-47058-6_3 · Cited by 82 · see citing works
Heyduk, E., & Heyduk, T. (1997) · Analytical Biochemistry · doi:10.1006/abio.1997.2148 · Cited by 80 · see citing works
Thiol-Reactive Luminescent Chelates of Terbium and Europium
Chen, J., & Selvin, P. R. (1999) · Bioconjugate Chemistry · doi:10.1021/bc980113w · Cited by 77 · see citing works
Self-citationLoo, T. W., & Clarke, D. M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103498200 · Cited by 70 · see citing works
Heyduk, T., & Heyduk, E. (2001) · Analytical Biochemistry · doi:10.1006/abio.2000.4925 · Cited by 69 · see citing works
Blomberg, K. R., Hurskainen, P., & Hemmilä, I. A. (1999) · Clinical Chemistry · doi:10.1093/clinchem/45.6.855 · Cited by 68 · see citing works
Heyduk, E., Heyduk, T., Claus, P. K., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.32.19763 · Cited by 63 · see citing works
Lifetime- and Color-Tailored Fluorophores in the Micro- to Millisecond Time Regime
Chen, J., & Selvin, P. R. (2000) · Journal of the American Chemical Society · doi:10.1021/ja9913986 · Cited by 60 · see citing works
Self-citationXiao, M., Li, H., Snyder, G. E., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15309 · Cited by 59 · see citing works
Sevéus, L., Väisälä, M., Hemmilä, I. A., et al. (1994) · Microscopy Research and Technique · doi:10.1002/jemt.1070280206 · Cited by 58 · see citing works
Mean DNA Bend Angle and Distribution of DNA Bend Angles in the CAP-DNA Complex in Solution
Kapanidis, A. N., Ebright, Y. W., Ludescher, R. D., et al. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4976 · Cited by 56 · see citing works
HOMOGENEOUS TIME-RESOLVED IL-2–IL-2Rα ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER
Stenroos, K., Hurskainen, P., Eriksson, S., et al. (1998) · Cytokine · doi:10.1006/cyto.1997.0321 · Cited by 53 · see citing works
Mathis, G., Socquet, F., Viguier, M., et al. (1997) · PubMed · Cited by 46 · see citing works
A New Twist in the Saga of Charge Movement in Voltage-Dependent Ion Channels
Horn, R. (2000) · Neuron · doi:10.1016/s0896-6273(00)81055-7 · Cited by 39 · see citing works
Root, D. D. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.11.5685 · Cited by 39 · see citing works
Heyduk, E., & Heyduk, T. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.6.3315 · Cited by 33 · see citing works
An improved instrument for measuring time-resolved lanthanide emission and resonance energy transfer
Xiao, M., & Selvin, P. R. (1999) · Review of Scientific Instruments · doi:10.1063/1.1150005 · Cited by 31 · see citing works
Self-citationSynthesis and spectral properties of a new luminescent europium(III) terpyridyl chelate
Cooper, M. E., & Sammes, P. G. (2000) · Journal of the Chemical Society Perkin Transactions 2 · doi:10.1039/b002322l · Cited by 21 · see citing works
Improvements in the Sensitivity of Time Resolved Fluorescence Energy Transfer Assays
Jones, S. G., Lee, D. Y., Wright, J. F., et al. (2001) · Journal of Fluorescence · doi:10.1023/a:1016691414432 · Cited by 18 · see citing works
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Degorce, F. (2009) · Current Chemical Genomics · doi:10.2174/1875397300903010022 · Cited by 501 · see citing works
Modulators of Protein–Protein Interactions
Milroy, L.-G., Grossmann, T. N., Hennig, S., Brunsveld, L., & Ottmann, C. (2014) · Chemical Reviews · doi:10.1021/cr400698c · Cited by 497 · see citing works
Doumazane, E., Scholler, P., Zwier, J. M., Trinquet, E., Rondard, P., & Pin, J. (2011) · The FASEB Journal · doi:10.1096/fj.10-163147 · Cited by 324 · see citing works
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Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114 · see citing works
Schäferling, M., & Nagl, S. (2011) · Methods in Molecular Biology · doi:10.1007/978-1-61779-043-0_19 · Cited by 22 · see citing works
- Authors
- Selvin, P. R.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Selvin, Paul R.
- Venue
- Annual Review of Biophysics and Biomolecular Structure
- Publisher
- Annual Reviews
- Published
- 2002-06
- Volume
- 31
- Issue
- 1
- Pages
- 275-302
- ISSN
- 1056-8700 (ISSN-L)
- 1545-4266
- OpenAlex
- W2124639076
- Cited by
- 549
- References
- 63
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 6 · 2023: 11 · 2022: 17 · 2021: 21 · 2020: 18 · 2019: 20 · 2018: 21 · 2017: 24 · 2016: 24 · 2015: 24 · 2014: 22 · 2013: 44 · 2012: 45
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annual Review of Biophysics and Biomolecular Structure (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 44420)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Lanthanide and Transition Metal Complexes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence Properties of Advanced Materials
- Keywords
- lanthanide luminescence, resonance energy transfer, donor molecules, ion channels, protein-protein interaction, protein-DNA complex, peptide dimerization, transcription factors, ligand-receptor interactions, high-throughput screening, living cells
- MeSH terms
- Animals, Biophysics, DNA, Energy Transfer, Ligands, Models, Biological, Models, Chemical, Spectrometry, Fluorescence, Models, Statistical, Protein Structure, Tertiary, Dimerization, Lanthanoid Series Elements
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