Cited by 549Lanthanide and Transition Metal ComplexesAdvanced Fluorescence Microscopy TechniquesLuminescence Properties of Advanced Materials

Principles and Biophysical Applications of Lanthanide-Based Probes

Selvin, Paul R.·2002·Annual Review of Biophysics and Biomolecular Structure

Abstract

▪ Abstract Using luminescent lanthanides, instead of conventional fluorophores, as donor molecules in resonance energy transfer measurements offers many technical advantages and opens up a wide range of new applications. Advantages include farther measurable distances (∼100 Å) with greater accuracy, insensitivity to incomplete labeling, and the ability to use generic relatively large labels, when necessary. Applications highlighted include the study of ion channels in living cells, protein-protein interaction in cells, DNA-protein complexes, and high-throughput screening assays to measure peptide dimerization associated with DNA transcription factors and ligand-receptor interactions.

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Crossref · 10.1146/annurev.biophys.31.101101.140927

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Authors
Selvin, P. R.
Affiliations
University of Illinois Urbana-Champaign (US)
Corresponding author
Selvin, Paul R.
Venue
Annual Review of Biophysics and Biomolecular Structure
Published
2002-06
Volume
31
Issue
1
Pages
275-302
ISSN
  • 1056-8700 (ISSN-L)
  • 1545-4266
OpenAlex
W2124639076
Cited by
549
References
63
Citations by year
2026: 5 · 2025: 8 · 2024: 6 · 2023: 11 · 2022: 17 · 2021: 21 · 2020: 18 · 2019: 20 · 2018: 21 · 2017: 24 · 2016: 24 · 2015: 24 · 2014: 22 · 2013: 44 · 2012: 45
FWCI
0.99
Type
journal article
Language
en
Open access
closed
Locations
  • Annual Review of Biophysics and Biomolecular Structure (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Energy
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 44420)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Lanthanide and Transition Metal Complexes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence Properties of Advanced Materials
Keywords
lanthanide luminescence, resonance energy transfer, donor molecules, ion channels, protein-protein interaction, protein-DNA complex, peptide dimerization, transcription factors, ligand-receptor interactions, high-throughput screening, living cells
MeSH terms
Animals, Biophysics, DNA, Energy Transfer, Ligands, Models, Biological, Models, Chemical, Spectrometry, Fluorescence, Models, Statistical, Protein Structure, Tertiary, Dimerization, Lanthanoid Series Elements

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