Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer
Abstract
A homogeneous assay method based on the long-lived fluorescence of rare earth cryptates and amplification by nonradiative energy transfer has been developed for immunoassays. The principles of the assay allow a double discrimination of the emitted signal through spectral and temporal selectivity. The cage structure of the complex, ion pairing around europium, as well as double-wavelength detection, fully shield the assay from perturbations of media. Events based on short-range interactions involving biomolecules are of tremendous importance in many domains of biology, either for analytical purposes or for molecular mechanism studies. Therefore, the principles and the reagents used to devise this homogeneous assay were adapted to various models representative of molecular and cellular processes and were chosen from the signaling pathways involved in cellular communication and expression: epidermal growth factor (EGF) receptor-ligand interaction, EGF receptor kinase activity, Jun/Fos protein-protein interaction, and DNA hybridization. Evaluation of the homogeneous assays yielded results compatible with those from comparison assays and demonstrates the versatility and wide range of applicability of this methodology.
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- Authors
- Mathis, G.
- Affiliations
- CIS Bio International, Bagnols sur Cèze, France
- Corresponding author
- Mathis, G
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-09-01
- Volume
- 41
- Issue
- 9
- Pages
- 1391-1397
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2142115916
- Cited by
- 339
- References
- 36
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 10 · 2020: 5 · 2019: 4 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 17 · 2014: 12 · 2013: 13 · 2012: 15
- FWCI
- 11.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Lanthanide and Transition Metal Complexes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- europium complexes, fluorescence resonance energy transfer, homogeneous immunoassay, nonradiative energy transfer, DNA hybridization, molecular interactions
- MeSH terms
- Bridged Bicyclo Compounds, Energy Transfer, Epidermal Growth Factor, Europium, Fluorescent Dyes, Humans, Nucleic Acid Hybridization, Organometallic Compounds, Receptor Protein-Tyrosine Kinases, ErbB Receptors
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