The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines
Abstract
Maintenance of intestinal mucosal epithelial integrity requires polyamines that are involved in the multiple signaling pathways controlling gene expression and different epithelial cell functions. Integrity of the intestinal epithelial barrier depends on a complex of proteins composing different intercellular junctions, including tight junctions, adherens junctions, and desmosomes. E-cadherin is primarily found at the adherens junctions and plays a critical role in cell-cell adhesions that are fundamental to formation of the intestinal epithelial barrier. The current study determined whether polyamines regulate intestinal epithelial barrier function by altering E-cadherin expression. Depletion of cellular polyamines by alpha-difluoromethylornithine (DFMO) reduced intracellular free Ca2+ concentration ([Ca2+]cyt), decreased E-cadherin expression, and increased paracellular permeability in normal intestinal epithelial cells (IEC-6 line). Polyamine depletion did not alter expression of tight junction proteins such as zona occludens (ZO)-1, ZO-2, and junctional adhesion molecule (JAM)-1. Addition of exogenous polyamine spermidine reversed the effects of DFMO on [Ca2+]cyt and E-cadherin expression and restored paracellular permeability to near normal. Elevation of [Ca2+]cyt by the Ca2+ ionophore ionomycin increased E-cadherin expression in polyamine-deficient cells. In contrast, reduction of [Ca2+]cyt by polyamine depletion or removal of extracellular Ca2+ not only inhibited expression of E-cadherin mRNA but also decreased the half-life of E-cadherin protein. These results indicate that polyamines regulate intestinal epithelial paracellular barrier function by altering E-cadherin expression and that polyamines are essential for E-cadherin expression at least partially through [Ca2+]cyt.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It was shown that depletion of cellular polyamines by α-difluoromethylornithine reduced intracellular free Ca2+ concentrations in normal IECs (IEC-6 cell line),127 which leads to decreased expression of E-cadherin and increased paracellular permeability.”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [127]
In the citing paper
“These studies suggested that polyamines regulate gut barrier function through upregulating E-cadherin in an intracellular Ca2+-dependent manner.127”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [127]
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cell Adhesion: The Molecular Basis of Tissue Architecture and Morphogenesis
Gumbiner, B. M. (1996) · Cell · doi:10.1016/s0092-8674(00)81279-9 · Cited by 3,471
Tabor, C. W., & Tabor, H. (1984) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.53.070184.003533 · Cited by 3,111
Store depletion and calcium influx
Parekh, A. B., & Penner, R. (1997) · Physiological Reviews · doi:10.1152/physrev.1997.77.4.901 · Cited by 1,398
CADHERINS: A MOLECULAR FAMILY IMPORTANT IN SELECTIVE CELL-CELL ADHESION
Takeichi, M. (1990) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.59.070190.001321 · Cited by 1,301
MOLECULAR AND FUNCTIONAL ANALYSIS OF CADHERIN-BASED ADHERENS JUNCTIONS
Yap, A. S., Brieher, W. M., & Gumbiner, B. M. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.119 · Cited by 818
Gumbiner, B., Stevenson, B., & Grimaldi, A. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1575 · Cited by 799
Quaroni, A., Wands, J. R., Trelstad, R. L., & Isselbacher, K. J. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.80.2.248 · Cited by 778
Rimm, D. L., Koslov, E. R., Kebriaei, P., Cianci, C. D., & Morrow, J. S. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.19.8813 · Cited by 735
Cited by
Sorted by citations
Polyamines and cancer: old molecules, new understanding
Gerner, E. W., & Meyskens, F. L. (2004) · Nature reviews. Cancer · doi:10.1038/nrc1454 · Cited by 1,163
The Human Gut Microbiome – A Potential Controller of Wellness and Disease
Kho, Z. Y., & Lal, S. K. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01835 · Cited by 1,180
Postler, T. S., & Ghosh, S. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.05.008 · Cited by 876
“…of ornithine decarboxylase negatively affects maintenance, repair, and function of the intestinal epithelium, although it is not clear if or how this relates to dietary and microbially derived polyamine absorption (Chen et al., 2007; Guo et al., 2003, 2005; Liu et al., 2009; Lux et al., 1980).”
BackgroundRegulation of Intestinal Barrier Function by Microbial Metabolites
Ghosh, S., Whitley, C. S., Haribabu, B., & Jala, V. R. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.02.007 · Cited by 835
Recent advances in chitosan-based nanoparticles for oral delivery of macromolecules
Chen, M., Mi, F., Liao, Z., Hsiao, C., Sonaje, K., Chung, M., Hsu, L., & Sung, H. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.10.010 · Cited by 453
Metadata
- Authors
- Guo, X., Rao, J. N., Liu, L., Zou, T.-T., Turner, D. J., Bass, B. L., & Wang, J.-Y.
- Affiliations
- University of Maryland, Baltimore (US)
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2003-11
- Volume
- 285
- Issue
- 5
- Pages
- C1174-C1187
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2109680774
- Cited by
- 153
- References
- 66
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 6 · 2021: 4 · 2020: 6 · 2019: 2 · 2018: 2 · 2017: 6 · 2016: 11 · 2015: 9 · 2014: 9 · 2013: 6 · 2012: 9
- FWCI
- 3.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK061972, (DK-57819, DK-61972, R01DK057819)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- polyamines, adherens junctions, paracellular permeability, intestinal epithelial barrier, E-cadherin, intracellular calcium, difluoromethylornithine, spermidine, tight junctions, protein stability, IEC-6 cells
- MeSH terms
- Animals, Calcium, Cell Line, Cell Membrane Permeability, Epithelial Cells, Intestinal Mucosa, Polyamines, Cadherins, Adherens Junctions, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
