The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method
Abstract
A calcein fluorescence quenching method was applied to measure osmotic water permeability in highly differentiated primary cultures of brain astrocytes from wild-type and aquaporin-4 (AQP-4)-deficient mice. Cells grown on coverglasses were loaded with calcein for measurement of volume changes after osmotic challenge. Hypotonic shock producing twofold cell swelling resulted in a reversible ∼12% increase in calcein fluorescence, which was independent of cytosolic calcein concentration at levels well below where calcein self-quenching occurs. Calcein fluorescence was quenched in <200 ms in response to addition of cytosol in vitro, indicating that the fluorescence signal arises from changes in cytosol concentration. In astrocytes from wild-type CD1 mice, calcein fluorescence increased reversibly in response to hypotonic challenge with a half-time of 0.92 ± 0.05 s at 23°C, corresponding to an osmotic water permeability ( P f) of ∼0.05 cm/s. P f was reduced 7.1-fold in astrocytes from AQP-4-deficient mice. Temperature dependence studies indicated an increased Arrhenius activation energy for water transport in AQP-4-deficient astrocytes (11.3 ± 0.5 vs. 5.5 ± 0.4 kcal/mol). Our studies establish a calcein quenching method for measurement of cell membrane water permeability and indicate that AQP-4 provides the principal route for water transport in astrocytes.
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Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke
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Self-citationNielsen, S., Arnulf Nagelhus, E., Amiry-Moghaddam, M., Bourque, C., Agre, P., & Petter Ottersen, O. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-01-00171.1997 · Cited by 1,442
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Self-citationNeuronal Regulation of Glutamate Transporter Subtype Expression in Astrocytes
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Self-citationKao, H. P., & Verkman, A. S. (1994) · Biophysical Journal · doi:10.1016/s0006-3495(94)80601-0 · Cited by 420
Self-citationFrigeri, A., Gropper, M. A., Umenishi, F., Kawashima, M., Brown, D., & Verkman, A. S. (1995) · Journal of Cell Science · doi:10.1242/jcs.108.9.2993 · Cited by 400
Self-citationAquaporin-4 expression is increased in oedematous human brain tumours
Saadoun, S. (2002) · Journal of Neurology, Neurosurgery & Psychiatry · doi:10.1136/jnnp.72.2.262 · Cited by 376
Fluorescence correlation spectroscopy: Diagnostics for sparse molecules
Maiti, S., Haupts, U., & Webb, W. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.22.11753 · Cited by 317
Impaired Hearing in Mice Lacking Aquaporin-4 Water Channels
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Cited by
Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
A new look at cerebrospinal fluid circulation
Brinker, T., Stopa, E., Morrison, J., & Klinge, P. (2014) · Fluids and Barriers of the CNS · doi:10.1186/2045-8118-11-10 · Cited by 819
Physiological Roles of Aquaporin-4 in Brain
Nagelhus, E. A., & Ottersen, O. P. (2013) · Physiological Reviews · doi:10.1152/physrev.00011.2013 · Cited by 766
The molecular basis of water transport in the brain
Amiry-Moghaddam, M., & Ottersen, O. P. (2003) · Nature Reviews Neuroscience · doi:10.1038/nrn1252 · Cited by 733
Molecular pathophysiology of cerebral edema
Stokum, J. A., Gerzanich, V., & Simard, J. M. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x15617172 · Cited by 587
Metadata
- Authors
- Solenov, E., Watanabe, H., Manley, G. T., & Verkman, A. S.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2004-02
- Volume
- 286
- Issue
- 2
- Pages
- C426-C432
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2046611979
- Cited by
- 329
- References
- 37
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Solenov, E., Watanabe, H., Manley, G. T., & Verkman, A. S. (2004). Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.00298.2003
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