IFN-γ induces lipogenesis in mouse mesangial cells via the JAK2/STAT1 pathway
Abstract
The objective is to investigate the effect of high mobility group box-1 (HMGB1) on lipid deposition in γ-interferon (IFN-γ)-stimulated mouse mesangial cell line (MMC) and to determine whether the Janus kinase 2 and signal transducer and activator of transcription 1 (JAK2/STAT1) signaling pathway plays an important role in this process. We employed a control group, an IFN-γ stimulation group, and an IFN-γ + AG490 (JAK2 inhibitor) group. RNA interference aimed at sterol regulatory element-binding protein-1 (SREBP-1) or HMGB1 was used to investigate the effect of these proteins on IFN-γ-induced lipid deposition. Western blotting was used to detect phospho (p)-JAK2, JAK2, p-STAT1, STAT1, SREBP-1, fatty acid synthase (FAS), and HMGB1 protein expression. RT-PCR was used to detect SREBP-1, FAS, and HMGB1 mRNA. Oil Red O staining and the triglyceride assay were used to detect lipid deposition and triglyceride content. Results were as follows: 1) IFN-γ increased MMC cell lipid deposition, triglyceride content, and p-JAK2, p-STAT1, SREBP-1, and FAS expression; 2) SREBP-1 inhibition prevented FAS upregulation and attenuated IFN-γ-induced MMC cell lipid deposition and triglyceride content; 3) HMGB1 upregulated SREBP-1 and FAS mRNA and protein levels, which increased lipid deposition in MMC cells. Small interfering RNA-mediated inhibition of HMGB1 decreased SREBP-1 and FAS expression and lipid accumulation; 4) AG490 decreased upregulation of HMGB1 and p-JAK2/p-STAT1, as well as IFN-γ-induced lipogenesis. In conclusion, the JAK2/STAT1 pathway mediates IFN-γ-induced lipogenesis in MMC cells through regulation of HMGB1/SREBP-1/FAS.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Between IFN-β and control conditions in all cells, the top 25 differentially active TFs, including the top-ranked SREBP, are consistent with previously reported interferon induction of lipogenesis.
“Between IFN-β and control conditions in all cells, the top 25 differentially active TFs, including the top-ranked SREBP (Supplementary Fig. 16d), are consistent with previously reported interferon induction of lipogenesis57, which has not been described in the previous scRNA-seq analysis56.”
From Transformer for one stop interpretable cell type annotation (2023) · cites this paper as [57]
Cites
Tsokos, G. C. (2016) · Elsevier eBooks · doi:10.1016/b978-0-323-37754-6.50094-4 · Cited by 3,990 · see citing works
Darnell, J. E. (1997) · Science · doi:10.1126/science.277.5332.1630 · Cited by 3,979 · see citing works
JAK-STAT Signaling: From Interferons to Cytokines
Schindler, C., Levy, D. E., & Decker, T. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.r700016200 · Cited by 1,263 · see citing works
Murine Models of Systemic Lupus Erythematosus
Perry, D. J., Sang, A. L., Yin, Y., Zheng, Y.-Y., & Morel, L. M. (2011) · BioMed Research International · doi:10.1155/2011/271694 · Cited by 379 · see citing works
Sun, L., Halaihel, N., Zhang, W., Rogers, T. E., & Levi, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m110650200 · Cited by 330 · see citing works
Borba, E. F., & Bonfá, E. (1997) · Lupus · doi:10.1177/096120339700600610 · Cited by 289 · see citing works
Pisetsky, D. S., Erlandsson-Harris, H., & Andersson, U. (2008) · Arthritis Research & Therapy · doi:10.1186/ar2440 · Cited by 188 · see citing works
Svenungsson, E., Gunnarsson, I., Fei, G., Lundberg, I. E., Klareskog, L., & Frostegård, J. (2003) · Arthritis & Rheumatism · doi:10.1002/art.11264 · Cited by 164 · see citing works
Elevated Th17 cells are accompanied by FoxP3+ Treg cells decrease in patients with lupus nephritis
Xing, Q., Wang, B., Su, H., et al. (2011) · Rheumatology International · doi:10.1007/s00296-010-1771-0 · Cited by 138 · see citing works
Schindler, C., & Strehlow, I. (1999) · Advances in pharmacology · doi:10.1016/s1054-3589(08)60111-8 · Cited by 129 · see citing works
Kutty, R. K., Nagineni, C. N., Samuel, W., Vijayasarathy, C., Hooks, J. J., & Redmond, T. M. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.10.042 · Cited by 121 · see citing works
Yoshizaki, A., Komura, K., Iwata, Y., Ogawa, F., Hara, T., Muroi, E., Takenaka, M., Shimizu, K., Hasegawa, M., Fujimoto, M., et al. (2008) · Journal of Clinical Immunology · doi:10.1007/s10875-008-9252-x · Cited by 111 · see citing works
Kuniyasu, H., Yano, S., Sasaki, T., Sasahira, T., Sone, S., & Ohmori, H. (2005) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)62296-1 · Cited by 110 · see citing works
Bacterial Endotoxin Induces the Release of High Mobility Group Box 1 via the IFN-β Signaling Pathway
Kim, J., Kim, S.-J., Lee, I., Lee, M., Uematsu, S., Akira, S., & Oh, K. I. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0801364 · Cited by 99 · see citing works
Systemic lupus erythematosus and “lupus dyslipoproteinemia”
de Carvalho, J. F., Bonfá, E., & Borba, E. F. (2007) · Autoimmunity Reviews · doi:10.1016/j.autrev.2007.11.016 · Cited by 99 · see citing works
Sheu, M., Chao, K. F., Sung, Y. J., Lin, W., Lin‐Shiau, S. Y., & Liu, S. (2004) · Cellular Signalling · doi:10.1016/j.cellsig.2004.11.015 · Cited by 62 · see citing works
Self-citationSegal, R., Dayan, M., Globerson, A., et al. (1997) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(97)01891-5 · Cited by 57 · see citing works
Hao, J., Zhu, L., Zhao, S., et al. (2011) · Experimental Cell Research · doi:10.1016/j.yexcr.2011.02.003 · Cited by 51 · see citing works
Self-citationNajima, Y., Yahagi, N., Takeuchi, Y., Matsuzaka, T., Sekiya, M., Nakagawa, Y., Amemiya-Kudo, M., Okazaki, H., Okazaki, S., Tamura, Y., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m414549200 · Cited by 47 · see citing works
Telles, R. W., Lanna, C., Ferreira, G. A., & Ribeiro, A. L. P. (2010) · Lupus · doi:10.1177/0961203309359781 · Cited by 46 · see citing works
Cytokine imbalance in systemic lupus erythematosus: a study on northern Indian subjects
Arora, V., Verma, J., Marwah, V., Kumar, A., Anand, D., & Das, N. (2012) · Lupus · doi:10.1177/0961203311434937 · Cited by 40 · see citing works
High Mobility Group Box–1 as a Therapeutic Target Downstream of Tumor Necrosis Factor
Czura, C. J., Yang, H., & Tracey, K. J. (2003) · The Journal of Infectious Diseases · doi:10.1086/374753 · Cited by 32 · see citing works
Hao, J., Liu, S., Zhao, S., et al. (2011) · British Journal Of Nutrition · doi:10.1017/s0007114511002613 · Cited by 32 · see citing works
Self-citationFeng, X., Hao, J., Liu, Q., Yang, L., Lv, X., Zhang, Y., Xing, L., Xu, N., & Liu, S. (2012) · Journal of Cellular Biochemistry · doi:10.1002/jcb.24071 · Cited by 32 · see citing works
Self-citationHua, X., Su, J., Svenungsson, E., Hurt‐Camejo, E., Jensen‐Urstad, K., Angelin, B., Båvenholm, P., & Frostegård, J. (2009) · Scandinavian Journal of Rheumatology · doi:10.1080/03009740802541470 · Cited by 30 · see citing works
Impaired JAK2-induced activation of STAT3 in failing human myocytes.
Cambi, G., Lucchese, G., Djeokeng, M. M. H., Modesti, A., Fiaschi, T., Faggian, G., Sani, G., & Modesti, P. (2012) · Molecular Biosystems · doi:10.1039/c2mb25120e · Cited by 27 · see citing works
Elevated levels of HMGB1 in cancerous and inflammatory effusions.
Winter, N., Meyer, A., Richter, A., Krisponeit, D., & Bullerdiek, J. (2009) · PubMed · Cited by 18 · see citing works
Bennemann, K., Galm, O., Wilop, S., Schubert, C., Brümmendorf, T. H., & Jost, E. (2012) · Clinical Epigenetics · doi:10.1186/1868-7083-4-12 · Cited by 10 · see citing works
Johnson, H. M. (2011) · Journal of Clinical & Cellular Immunology · doi:10.4172/2155-9899.1000112 · Cited by 6 · see citing works
[The effect of abnormal lipid metabolism to glomerular injury].
Kondo, S., Yoshizawa, N., Oshima, S., et al. (1991) · PubMed · Cited by 1 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Kang, R., Chen, R., Zhang, Q., Hou, W., Wu, S., Cao, L., Huang, J., Yu, Y., Fan, X.-G., Yan, Z., et al. (2014) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2014.05.001 · Cited by 956 · see citing works
Transformer for one stop interpretable cell type annotation
Chen, J., Xu, H., Tao, W., Chen, Z., Zhao, Y., & Han, J.-D. J. (2023) · Nature Communications · doi:10.1038/s41467-023-35923-4 · Cited by 189 · see citing works
- Authors
- Hao, J., Zhang, Y.-J., Lv, X., Xu, N., Liu, Q.-J., Zhao, S., Feng, X.-J., Xing, L.-L., Kang, P.-P., Li, G.-Y., & Liu, S.-X.
- Affiliations
- Hebei Medical University (CN)
- Department of Pathology, Hebei Medical University, Hebei Key Laboratory of Nephrology, Shijiazhuang, China;
- Department of Nephrology, Second Hospital of Hebei Medical University, Shijiazhuang, China; and
- Department of Nephrology, Third Hospital of Hebei Medical University, Shijiazhuang, China
- Corresponding author
- Liu, Qin-juan
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2013-04-15
- Volume
- 304
- Issue
- 8
- Pages
- C760-C767
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2322101694
- Cited by
- 27
- References
- 30
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2016: 3 · 2015: 2 · 2014: 2
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- interferon-gamma, lipogenesis, mesangial cells, JAK2/STAT1 signaling, HMGB1, SREBP1, fatty acid synthase, lipid accumulation, triglyceride accumulation, diabetic nephropathy
- MeSH terms
- Animals, Cell Line, Interferon-gamma, Signal Transduction, HMGB1 Protein, Lipogenesis, Mesangial Cells, STAT1 Transcription Factor, Mice, Janus Kinase 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
