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Fasting for 72 h increases intramyocellular lipid content in nondiabetic, physically fit men
Abstract
The purpose of this study was to determine changes in intramyocellular lipid (IMCL) content in the vastus lateralis of nondiabetic, physically fit males over 72 h of fasting. Six men, mean age 35 yr (range 23–55 yr), body mass index 23.7 kg/m 2(21.2–27.4 kg/m 2), undertook a water-only fast for 84 h. Vastus lateralis IMCL content was determined using proton magnetic resonance spectroscopy after 12 and 84 h of fasting. Venous blood was sampled at 12-h intervals throughout the fast. IMCL-(CH 2)n/water and IMCL-(CH 2)n/total creatine ratios increased from 0.00623 ± 0.00065 to 0.0142 ± 0.0015 ( P = 0.002) and 6.82 ± 0.87 to 14.96 ± 1.73 ( P = 0.001), respectively. Plasma free fatty acid (FFA), serum triglyceride, and whole blood 3-hydroxybutyrate concentrations increased ( P < 0.001, <0.05, <0.03, respectively), whereas plasma glucose and serum insulin concentrations decreased (both P < 0.001) during fasting. In conclusion, 72-h water-only fasting produces a large increase in plasma FFA concentration, a drop in serum insulin concentration, and accumulation of IMCL in the vastus lateralis muscle of nondiabetic, physically fit men.
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“the ∼2.7-fold increase in IMTG levels after 60 h of fasting is slightly higher in comparison with previous reports (12,39).”
From Prolonged fasting identifies skeletal muscle mitochondrial dysfunction as consequence rather than cause of human insulin resistance (2010) · cites this paper as [12]
In the citing paper
“The impressive increase in plasma FFA upon prolonged fasting is in line with previous findings in humans (12,39)”
From Prolonged fasting identifies skeletal muscle mitochondrial dysfunction as consequence rather than cause of human insulin resistance (2010) · cites this paper as [12]
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Metadata
- Authors
- Stannard, S. R., Thompson, M. W., Fairbairn, K., Huard, B., Sachinwalla, T., & Thompson, C. H.
- Affiliations
- The University of Sydney (AU)
- Rayscan Imaging, Liverpool 2170, New South Wales, Australia
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2002-12-01
- Volume
- 283
- Issue
- 6
- Pages
- E1185-E1191
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2115449275
- Cited by
- 108
- References
- 48
- Citations by year
- 2026: 6 · 2024: 2 · 2023: 9 · 2022: 2 · 2021: 3 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 4
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Dietary Effects on Health
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- water-only fasting, intramyocellular lipid, vastus lateralis, plasma free fatty acids, insulin, ketone bodies, beta-hydroxybutyrate, serum triglycerides, muscle lipid accumulation, caloric restriction, physical fitness
- MeSH terms
- Adult, Blood Glucose, Fasting, Fatty Acids, Nonesterified, Humans, Insulin, Intracellular Fluid, Leg, Magnetic Resonance Imaging, Male, Middle Aged, Magnetic Resonance Spectroscopy, Physical Fitness, Reference Values, Time Factors, Triglycerides, Muscle, Skeletal, 3-Hydroxybutyric Acid, Lipid Metabolism
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