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Myofibrillar and collagen protein synthesis in human skeletal muscle in young men after maximal shortening and lengthening contractions
Abstract
We aimed to determine whether there were differences in the extent and time course of skeletal muscle myofibrillar protein synthesis (MPS) and muscle collagen protein synthesis (CPS) in human skeletal muscle in an 8.5-h period after bouts of maximal muscle shortening (SC; average peak torque = 225 +/- 7 N.m, means +/- SE) or lengthening contractions (LC; average peak torque = 299 +/- 18 N.m) with equivalent work performed in each mode. Eight healthy young men (21.9 +/- 0.6 yr, body mass index 24.9 +/- 1.3 kg/m2) performed 6 sets of 10 maximal unilateral LC of the knee extensors on an isokinetic dynamometer. With the contralateral leg, they then performed 6 sets of maximal unilateral SC with work matched to the total work performed during LC (10.9 +/- 0.7 vs. 10.9 +/- 0.8 kJ, P = 0.83). After exercise, the participants consumed small intermittent meals to provide 0.1 g.kg(-1).h(-1) of protein and carbohydrate. Prior exercise elevated MPS above rest in both conditions, but there was a more rapid rise after LC (P < 0.01). The increases (P < 0.001) in CPS above rest were identical for both SC and LC and likely represent a remodeling of the myofibrillar basement membrane. Therefore, a more rapid rise in MPS after maximal LC could translate into greater protein accretion and muscle hypertrophy during chronic resistance training utilizing maximal LC.
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“Skeletal muscle protein synthesis signaling, via activation of the mTORC1 pathway, is stimulated in the range of 1–7 days post-damage in response to various eccentric or lengthening contraction protocols in rodents and humans (57–61).”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [60]
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Metadata
- Authors
- Moore, D. R., Phillips, S. M., Babraj, J. A., Smith, K., & Rennie, M. J.
- Affiliations
- McMaster University (CA)
- University of Dundee (GB)
- University of Nottingham (GB)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2005-06
- Volume
- 288
- Issue
- 6
- Pages
- E1153-E1159
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2125844006
- Cited by
- 268
- References
- 25
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 8 · 2023: 10 · 2022: 5 · 2021: 16 · 2020: 13 · 2019: 15 · 2018: 7 · 2017: 21 · 2016: 15 · 2015: 15 · 2014: 13 · 2013: 14 · 2012: 17
- FWCI
- 3.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
- Keywords
- myofibrillar protein synthesis, skeletal muscle, eccentric contraction, shortening contractions, muscle hypertrophy, muscle remodeling, resistance training, knee extensors
- MeSH terms
- Adult, Amino Acids, Blood Chemical Analysis, Blood Glucose, Collagen, Histocytochemistry, Humans, Insulin, Leg, Male, Muscle Contraction, Muscle Proteins, Myofibrils, Oxygen Consumption, Exercise, Muscle, Skeletal
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