Adrenergically stimulated blood flow in brown adipose tissue is not dependent on thermogenesis
Abstract
Brown adipose tissue (BAT) thermogenesis relies on blood flow to be supplied with nutrients and oxygen and for the distribution of the generated heat to the rest of the body. Therefore, it is fundamental to understand the mechanisms by which blood flow is regulated and its relation to thermogenesis. Here, we present high-resolution laser-Doppler imaging (HR-LDR) as a novel method for noninvasive in vivo measurement of BAT blood flow in mice. Using HR-LDR, we found that norepinephrine stimulation increases BAT blood flow in a dose-dependent manner and that this response is profoundly modulated by environmental temperature acclimation. Surprisingly, we found that mice lacking uncoupling protein 1 (UCP1) have fully preserved BAT blood flow response to norepinephrine despite failing to perform thermogenesis. BAT blood flow was not directly correlated to systemic glycemia, but glucose injections could transiently increase tissue perfusion. Inguinal white adipose tissue, also known as a brite/beige adipose tissue, was also sensitive to cold acclimation and similarly increased blood flow in response to norepinephrine. In conclusion, using a novel noninvasive method to detect BAT perfusion, we demonstrate that adrenergically stimulated BAT blood flow is qualitatively and quantitatively fully independent of thermogenesis, and therefore, it is not a reliable parameter for the estimation of BAT activation and heat generation.
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Blood flow through BAT can be quickly increased (hyperemia).
“In addition, blood flow through BAT can be quickly increased (hyperemia) (Abreu-Vieira et al., 2015), and, in the setting of acute cold exposure, is optimal for quickly circulating warmed blood, or, in this case, a stress hormone.”
From Origin and function of stress-induced IL-6 in murine models (2020) · cites this paper as Abreu-Vieira et al., 2015
BAT hyperemia and thermogenesis can be decoupled.
“Interestingly, BAT hyperemia and thermogenesis can be decoupled, suggesting that there could be scenarios were uncoupled respiration is not necessary for hyperemia (Abreu-Vieira et al., 2015).”
From Origin and function of stress-induced IL-6 in murine models (2020) · cites this paper as Abreu-Vieira et al., 2015
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Origin and function of stress-induced IL-6 in murine models
Qing, H., Desrouleaux, R., Israni-Winger, K., Mineur, Y. S., Fogelman, N., Zhang, C., Rashed, S., Palm, N. W., Sinha, R., Picciotto, M. R., et al. (2020) · Cell · doi:10.1016/j.cell.2020.05.054 · Cited by 303 · see citing works
- Authors
- Abreu-Vieira, G., Hagberg, C. E., Spalding, K. L., Cannon, B., & Nedergaard, J.
- Affiliations
- Stockholm University (SE)
- Karolinska Institutet (SE)
- Corresponding author
- Nedergaard, Jan
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2015-05-01
- Volume
- 308
- Issue
- 9
- Pages
- E822-E829
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W1997443498
- Cited by
- 34
- References
- 34
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 3 · 2018: 6 · 2017: 7 · 2016: 2 · 2015: 1
- FWCI
- 3.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Vetenskapsrådet (Swedish Research Council)
- DIABAT consortium (European Union Seventh Programme)
- Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)
- Novo Nordisk Foundation
- EC | European Research Council (ERC)
- Wilhelm och Else Stockmanns Stiftelse
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- brown adipose tissue, thermogenesis, tissue blood flow, norepinephrine, adrenergic stimulation, UCP1, cold acclimation, inguinal adipose tissue, laser Doppler imaging, tissue perfusion, glucose injection, heat generation
- MeSH terms
- Acclimatization, Animals, Body Composition, Adipose Tissue, Brown, Female, Hemodynamics, Male, Mice, Inbred C57BL, Norepinephrine, Regional Blood Flow, Laser-Doppler Flowmetry, Mice, Knockout, Adrenergic Agents, Thermogenesis, Mice
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