Cited by 1,263Adipose Tissue and MetabolismPeroxisome Proliferator-Activated ReceptorsAdipokines, Inflammation, and Metabolic Diseases

Chronic Peroxisome Proliferator-activated Receptor γ (PPARγ) Activation of Epididymally Derived White Adipocyte Cultures Reveals a Population of Thermogenically Competent, UCP1-containing Adipocytes Molecularly Distinct from Classic Brown Adipocytes

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Petrovic, Natasa, Walden, Tomas B., Shabalina, Irina G., Timmons, James A., Cannon, Barbara, Nedergaard, Jan·2010·Journal of Biological Chemistry

Abstract

The recent insight that brown adipocytes and muscle cells share a common origin and in this respect are distinct from white adipocytes has spurred questions concerning the origin and molecular characteristics of the UCP1-expressing cells observed in classic white adipose tissue depots under certain physiological or pharmacological conditions. Examining precursors from the purest white adipose tissue depot (epididymal), we report here that chronic treatment with the peroxisome proliferator-activated receptor gamma agonist rosiglitazone promotes not only the expression of PGC-1alpha and mitochondriogenesis in these cells but also a norepinephrine-augmentable UCP1 gene expression in a significant subset of the cells, providing these cells with a genuine thermogenic capacity. However, although functional thermogenic genes are expressed, the cells are devoid of transcripts for the novel transcription factors now associated with classic brown adipocytes (Zic1, Lhx8, Meox2, and characteristically PRDM16) or for myocyte-associated genes (myogenin and myomirs (muscle-specific microRNAs)) and retain white fat characteristics such as Hoxc9 expression. Co-culture experiments verify that the UCP1-expressing cells are not proliferating classic brown adipocytes (adipomyocytes), and these cells therefore constitute a subset of adipocytes ("brite" adipocytes) with a developmental origin and molecular characteristics distinguishing them as a separate class of cells.

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Crossref · 10.1074/jbc.m109.053942

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  • When stimulated by PPARγ agonist, brown fat cells emerging in white fat express molecular characteristics distinct from the interscapular brown fat cells.

    “When stimulated by PPARγ agonist, they express molecular characteristics distinct from the interscapular brown fat cells 93.”

    From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [93]

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Authors
Petrovic, N., Walden, T. B., Shabalina, I. G., Timmons, J. A., Cannon, B., & Nedergaard, J.
Affiliations
  • Stockholm University (SE)
  • Royal Veterinary College (GB)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2010-03
Volume
285
Issue
10
Pages
7153-7164
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1979026577
Cited by
1,263
References
61
Citations by year
2026: 21 · 2025: 29 · 2024: 39 · 2023: 44 · 2022: 67 · 2021: 85 · 2020: 94 · 2019: 79 · 2018: 99 · 2017: 118 · 2016: 111 · 2015: 114 · 2014: 114 · 2013: 121 · 2012: 63
FWCI
28.37
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion)
Funders
  • European Commission (201100)
  • Vetenskapsrådet
  • Biotechnology and Biological Sciences Research Council (BB/G013128/1)
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
PPARγ, rosiglitazone, brown adipocytes, beige adipocytes, thermogenesis, UCP1, epididymal adipose tissue, PGC-1α, mitochondrial biogenesis, adipocyte precursors, PRDM16, HOXC9, norepinephrine, adipogenesis
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Uncoupling Protein 1, Rosiglitazone, Animals, Cell Differentiation, Cells, Cultured, Epididymis, Gene Expression Regulation, Hypoglycemic Agents, Ion Channels, Male, Mice, Inbred C57BL, Oxygen Consumption, Transcription Factors, Biomarkers, Trans-Activators, Mice, Knockout, Coculture Techniques, Thermogenesis, Mitochondrial Proteins, Thiazolidinediones, PPAR gamma, Voltage-Dependent Anion Channels, Mice, Adipocytes, Brown, Adipocytes, White

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