CoChat
Sign Up
Cited by 111Nitric Oxide and Endothelin EffectsHemoglobin structure and functionHeme Oxygenase-1 and Carbon Monoxide

The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.

Heme proteins mediate the conversion of nitrite to nitric oxide in the vascular wall

Open access
Alzawahra, Wael F., Talukder, M. A. Hassan, Liu, Xiaoping, Samouilov, Alexandre, Zweier, Jay L.·2008·American Journal of Physiology-Heart and Circulatory Physiology
DOI 10.1152/ajpheart.00374.2008PMC PMC2519190
Open this paper in CoChat — free account

Abstract

Nitric oxide (NO) has been shown to be the endothelium-derived relaxing factor (EDRF), and its impairment contributes to a variety of cardiovascular disorders. Recently, it has been recognized that nitrite can be an important source of NO; however, questions remain regarding the activity and mechanisms of nitrite bioactivation in vessels and its physiological importance. Therefore, we investigated the effects of nitrite on in vivo hemodynamics in rats and in vitro vasorelaxation in isolated rat aorta under aerobic conditions. Studies were performed to determine the mechanisms by which nitrite is converted to NO. In anesthetized rats, nitrite dose dependently decreased both systolic and diastolic blood pressure with a threshold dose of 10 μM. Similarly, nitrite (10 μM-2 mM) caused vasorelaxation of aortic rings, and NO was shown to be the intermediate factor responsible for this activity. With the use of electrochemical as well as electron paramagnetic resonance (EPR) spectroscopy techniques NO generation was measured from isolated aortic vessels following nitrite treatment. Reduction of nitrite to NO was blocked by heating the vessel, suggesting that an enzymatic process is involved. Organ chamber experiments demonstrated that aortic relaxation induced by nitrite could be blocked by both hemoglobin and soluble guanylyl cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one (ODQ). In addition, both electrochemical and EPR spin-trapping measurements showed that ODQ inhibits nitrite-mediated NO production. These findings thus suggest that nitrite can be a precursor of EDRF and that sGC or other heme proteins inhibited by ODQ catalyze the reduction of nitrite to NO.

Overview

Integrity signals

Registry

Crossref · 10.1152/ajpheart.00374.2008

Cites

65 of 65 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 65 references

Cited by

Sorted by citations

See all 111

Related

Metadata

Authors
Alzawahra, W. F., Talukder, M. A. H., Liu, X., Samouilov, A., & Zweier, J. L.
Affiliations
  • The Ohio State University (US)
  • The Ohio State University Wexner Medical Center (US)
ORCID
Venue
American Journal of Physiology-Heart and Circulatory Physiology
Publisher
American Physiological Society
Published
2008-08
Volume
295
Issue
2
Pages
H499-H508
ISSN
  • 0363-6135 (ISSN-L)
  • 1522-1539
DOI
10.1152/ajpheart.00374.2008
PMC
PMC2519190
OpenAlex
W2111609140
Cited by
111
References
65
Citations by year
2026: 2 · 2025: 1 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 8 · 2015: 11 · 2014: 11 · 2013: 4 · 2012: 20
FWCI
7.24
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (HL-38324, R01HL063744, HL 65608, P01HL065608, HL 63744)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heme Oxygenase-1 and Carbon Monoxide
Keywords
nitrite, nitric oxide, heme proteins, endothelium-derived relaxing factor, soluble guanylate cyclase, hemoglobin, vasorelaxation, aorta, blood pressure, ODQ, spin trapping, cardiovascular physiology
MeSH terms
Soluble Guanylyl Cyclase, Animals, Aorta, Thoracic, Blood Pressure, Dose-Response Relationship, Drug, Electrochemistry, Electron Spin Resonance Spectroscopy, Enzyme Inhibitors, Guanylate Cyclase, Heart Rate, Hemeproteins, Infusions, Intravenous, Male, Nitric Oxide, Nitrites, Oxadiazoles, Oxyhemoglobins, Quinoxalines, Vasodilation, Signal Transduction, Rats, Sprague-Dawley, Receptors, Cytoplasmic and Nuclear, Endothelium-Dependent Relaxing Factors, Rats, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review