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Three-dimensional transmural organization of perimysial collagen in the heart
Open accessAbstract
There is strong support for the view that the ventricular myocardium has a laminar organization in which myocytes are grouped into branching layers separated by cleavage planes. However, understanding of the extent and functional implications of this architecture has been limited by the lack of a systematic three-dimensional description of the organization of myocytes and associated perimysial collagen. We imaged myocytes and collagen across the left ventricular wall at high resolution in seven normal rat hearts using extended volume confocal microscopy. We developed novel reconstruction and segmentation techniques necessary for the quantitative analysis of three-dimensional myocyte and perimysial collagen organization. The results confirm that perimysial collagen has an ordered arrangement and that it defines a laminar organization. Perimysial collagen is composed of three distinct forms: extensive meshwork on laminar surfaces, convoluted fibers connecting adjacent layers, and longitudinal cords. While myolaminae are the principal form of structural organization throughout most of the wall, they are not seen in the subepicardium, where perimysial collagen is present only as longitudinal cords.
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“In the native heart, overlapping myocardial fibers are arranged into distinct laminae four to six myocytes thick and separated from adjacent laminae by an extracellular collagen network42.”
From A tissue-engineered scale model of the heart ventricle (2018) · cites this paper as [42]
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Metadata
- Authors
- Pope, A. J., Sands, G. B., Smaill, B. H., & LeGrice, I. J.
- Affiliations
- University of Auckland (NZ)
- Venue
- American Journal of Physiology-Heart and Circulatory Physiology
- Publisher
- American Physiological Society
- Published
- 2008-09
- Volume
- 295
- Issue
- 3
- Pages
- H1243-H1252
- ISSN
- 0363-6135 (ISSN-L)
- 1522-1539
- PMC
- PMC2544485
- OpenAlex
- W2117433444
- Cited by
- 205
- References
- 51
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 4 · 2023: 8 · 2022: 9 · 2021: 16 · 2020: 16 · 2019: 14 · 2018: 12 · 2017: 10 · 2016: 11 · 2015: 15 · 2014: 12 · 2013: 15 · 2012: 18
- FWCI
- 7.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ResearchSpace (University of Auckland) (submittedVersion)
- Funders
- National Heart Foundation of New Zealand
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Keywords
- left ventricular wall, 3D reconstruction, collagen fiber arrangement, subepicardium, rat heart
- MeSH terms
- Animals, Collagen, Endocardium, Heart Ventricles, Image Processing, Computer-Assisted, Male, Myocardium, Pericardium, Rats, Inbred WKY, Rats, Wistar, Microscopy, Confocal, Myocytes, Cardiac, Rats
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