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Cited by 416

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Generation of superoxide in cardiomyocytes during ischemia before reperfusion

Becker, Lance B., vanden Hoek, Terry L., Shao, Zuo-Hui, Li, Chang-Qing, Schumacker, Paul T.·1999·American Journal of Physiology-Heart and Circulatory Physiology
DOI 10.1152/ajpheart.1999.277.6.h2240
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Abstract

Although a burst of oxidants has been well described with reperfusion, less is known about the oxidants generated by the highly reduced redox state and low O 2 of ischemia. This study aimed to further identify the species and source of these oxidants. Cardiomyocytes were exposed to 1 h of simulated ischemia while oxidant generation was assessed by intracellular dihydroethidine (DHE) oxidation. Ischemia increased DHE oxidation significantly (0.7 ± 0.1 to 2.3 ± 0.3) after 1 h. Myxothiazol (mitochondrial site III inhibitor) attenuated oxidation to 1.3 ± 0.1, as did the site I inhibitors rotenone (1.0 ± 0.1), amytal (1.1 ± 0.1), and the flavoprotein oxidase inhibitor diphenyleneiodonium (0.9 ± 0.1). By contrast, the site IV inhibitor cyanide, as well as inhibitors of xanthine oxidase (allopurinol), nitric oxide synthase (nitro-l-arginine methyl ester), and NADPH oxidase (apocynin), had no effect. Finally, DHE oxidation increased with Cu- and Zn-containing superoxide dismutase (SOD) inhibition using diethyldithiocarbamate (2.7 ± 0.1) and decreased with exogenous SOD (1.1 ± 0.1). We conclude that significant superoxide generation occurs during ischemia before reperfusion from the ubisemiquinone site of the mitochondrial electron transport chain.

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Metadata

Authors
Becker, L. B., vanden Hoek, T. L., Shao, Z.-H., Li, C.-Q., & Schumacker, P. T.
Venue
American Journal of Physiology-Heart and Circulatory Physiology
Publisher
American Physiological Society
Published
1999-12-01
Volume
277
Issue
6
Pages
H2240-H2246
ISSN
  • 0363-6135 (ISSN-L)
  • 1522-1539
DOI
10.1152/ajpheart.1999.277.6.h2240
OpenAlex
W2109821965
Cited by
416
References
39
FWCI
7.51
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Generation of superoxide in cardiomyocytes during ischemia before reperfusion. https://app.cochat.ai/papers/10.1152/ajpheart.1999.277.6.h2240
The paper
Becker, L. B., vanden Hoek, T. L., Shao, Z.-H., Li, C.-Q., & Schumacker, P. T. (1999). Generation of superoxide in cardiomyocytes during ischemia before reperfusion. American Journal of Physiology-Heart and Circulatory Physiology. https://doi.org/10.1152/ajpheart.1999.277.6.h2240

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.