Inducible binding of PU.1 and interacting proteins to the Toll-like receptor 4 promoter during endotoxemia

Pedchenko, Tetyana V.; Park, Gye Young; Joo, Myungsoo; Blackwell, Timothy S.; Christman, John W.

doi:10.1152/ajplung.00046.2005

Cited by 24Immune Response and InflammationGenomics and Chromatin DynamicsTuberculosis Research and Epidemiology

Inducible binding of PU.1 and interacting proteins to the Toll-like receptor 4 promoter during endotoxemia

Pedchenko, Tetyana V., Park, Gye Young, Joo, Myungsoo, Blackwell, Timothy S., Christman, John W.·2005·American Journal of Physiology-Lung Cellular and Molecular Physiology

DOI 10.1152/ajplung.00046.2005

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Abstract

We hypothesized that PU.1 and PU.1 interacting proteins (PIP) binding to the Toll-like receptor 4 (TLR4) promoter is involved in endotoxin-induced upregulation of TLR4 gene expression. Our results employing chromatin immunoprecipitation assays indicate that PU.1 binds to the murine TLR4 promoter both in macrophage cells and, most importantly, in whole lung tissue. Treatment of RAW 264.7 cells with endotoxin induced the association of PU.1 and the TLR4 promoter in a time-dependent manner, and this was closely tied to interactions between the TLR4 promoter and the PIP interferon regulatory factors (IRF)4 and IRF8. PU.1 binding was related to increases in steady-state TLR4 mRNA and total TLR4 protein in RAW cells. Endotoxemia in animals caused the similar inducible interaction between PU.1 and IRF4 and the TLR4 promoter in lung tissue of mice that was treated with a single intraperitoneal injection of endotoxin. PU.1 binding to the TLR4 promoter was not enhanced in the lung tissue of endotoxin-resistant C3H/HeJ mice in response to endotoxemia. Transient transfection studies in RAW cells indicate that inducible binding of PU.1 to the TLR4 promoter is abrogated by a Ser148 to Ala mutation in PU.1. These data suggest that induction of PU.1/PIP binding to the TLR4 promoter is involved in endotoxin response in vivo and may mediate transcriptional changes in TLR4 gene expression.

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“1 participates in regulating the expression of a variety of PRRs, including toll-like receptor (TLR)-4 [10], TLR-9 [11], mannose receptor (MR) [12], a dendritic cell-specific C-type lectin (DC-SIGN) [13], and Dectin-1 [14, 15].”
Background
Immune stress up regulates TLR4 and Tollip gene expression in the hypothalamus of ewes
Herman, A. P., Herman, A., Haziak, K., & Tomaszewska-Zaremba, D. (2013) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/66011/2013 · Cited by 14 · see citing works
NF-κB-Inducing Kinase Regulates Cyclooxygenase 2 Gene Expression in Macrophages by Phosphorylation of PU.1
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BackgroundMethodInfluentialSelf-citation
Single Nucleotide Polymorphisms in 5’ Upstream Region of Bovine TLR4 Gene Affecting Expression Profile and Transcription Factor Binding Sites
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Result
Genetic determination of the role of PU.1 in macrophage gene expression
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BackgroundSelf-citation
IRF4 Suppresses Osteogenic Differentiation of Periodontal Ligament Stem Cells by Activating IL‐18 Signaling Pathway in Periodontitis
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Regulación de la Expresión del Receptor Tipo Toll 4 (TLR4) por el Factor de Necrosis Tumoral α y Glucocorticoides
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Regulación de la expresión del Receptor Tipo Toll 4 (TLR4) por el Factor de Necrosis Tumoral α y Glucocorticoides
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Cutting Edge: Toll-Like Receptor 4 (TLR4)-Deficient Mice Are Hyporesponsive to Lipopolysaccharide: Evidence for TLR4 as the Lps Gene Product
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The design of transcription-factor binding sites is affected by combinatorial regulation
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Authors: Pedchenko, T. V., Park, G. Y., Joo, M., Blackwell, T. S., & Christman, J. W.
Affiliations: Vanderbilt University (US)
University of Illinois Chicago (US)
ORCID: Park, Gye Young
Joo, Myungsoo
Blackwell, Timothy S.
Christman, John W.
Venue: American Journal of Physiology-Lung Cellular and Molecular Physiology
Publisher: American Physiological Society
Published: 2005-09
Volume: 289
Issue: 3
Pages: L429-L437
ISSN: 1040-0605 (ISSN-L)
1522-1504
DOI: 10.1152/ajplung.00046.2005
OpenAlex: W2143029206
Cited by: 24
References: 52
Citations by year: 2024: 2 · 2020: 1 · 2019: 2 · 2017: 2 · 2016: 1 · 2014: 1 · 2013: 3
FWCI: 0.86
Type: journal article
Language: en
Open access: closed
Locations: American Journal of Physiology-Lung Cellular and Molecular Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
Keywords: PU.1, TLR4, endotoxemia, IRF4, IRF8, transcriptional regulation, lipopolysaccharide response, lung
MeSH terms: Interferon Regulatory Factor-4, Proto-Oncogene Protein Spi-1, Amino Acid Sequence, Animals, Cell Line, DNA-Binding Proteins, Endotoxins, Lung, Macrophages

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Inducible binding of PU.1 and interacting proteins to the Toll-like receptor 4 promoter during endotoxemia

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