An ex vivo model to induce early fibrosis-like changes in human precision-cut lung slices

Idiopathic pulmonary fibrosis

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Murine models of pulmonary fibrosis

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Acellular Normal and Fibrotic Human Lung Matrices as a Culture System for In Vitro Investigation

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Single-cell RNA sequencing identifies diverse roles of epithelial cells in idiopathic pulmonary fibrosis

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Increased stiffness of the rat liver precedes matrix deposition: implications for fibrosis

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WNT5A Is a Regulator of Fibroblast Proliferation and Resistance to Apoptosis

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Exploration of a potent PI3 kinase/mTOR inhibitor as a novel anti-fibrotic agent in IPF

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Shared and distinct mechanisms of fibrosis

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Senolytic drugs target alveolar epithelial cell function and attenuate experimental lung fibrosis ex vivo

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Use of precision cut lung slices as a translational model for the study of lung biology

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The WNT signaling pathways in wound healing and fibrosis

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Increased Extracellular Vesicles Mediate WNT5A Signaling in Idiopathic Pulmonary Fibrosis

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Interplay Between Keratinocytes and Fibroblasts: A Systematic Review Providing a New Angle for Understanding Skin Fibrotic Disorders

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Pathology and pathobiology of pulmonary hypertension: current insights and future directions

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Spray drying siRNA-lipid nanoparticles for dry powder pulmonary delivery

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Recent advances in our understanding of the mechanisms of lung alveolarization and bronchopulmonary dysplasia

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Pharmacotherapy and adjunctive treatment for idiopathic pulmonary fibrosis (IPF)

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Applications and Approaches for Three-Dimensional Precision-Cut Lung Slices. Disease Modeling and Drug Discovery

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Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis

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A lung targeted miR-29 mimic as a therapy for pulmonary fibrosis

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Saracatinib, a Selective Src Kinase Inhibitor, Blocks Fibrotic Responses in Preclinical Models of Pulmonary Fibrosis

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Neutrophilic inflammation during lung development disrupts elastin assembly and predisposes adult mice to COPD

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Novel drug delivery systems and disease models for pulmonary fibrosis

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Dual inhibition of αvβ6 and αvβ1 reduces fibrogenesis in lung tissue explants from patients with IPF

Decaris, M. L., Schaub, J. R., Chen, C., Cha, J., Lee, G. G., Rexhepaj, M., Ho, S. S., Rao, V., Marlow, M. M., Kotak, P., et al. (2021) · Respiratory Research · doi:10.1186/s12931-021-01863-0 · Cited by 94

Precision‐cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases

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Bioengineered 3D Models to Recapitulate Tissue Fibrosis

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Lung regeneration: a tale of mice and men

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Serum metabolic profiling identified a distinct metabolic signature in patients with idiopathic pulmonary fibrosis – a potential biomarker role for LysoPC

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Ex vivo tissue perturbations coupled to single-cell RNA-seq reveal multilineage cell circuit dynamics in human lung fibrogenesis

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Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis

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Human Pulmonary 3D Models For Translational Research

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Hyperoxia Injury in the Developing Lung Is Mediated by Mesenchymal Expression of Wnt5A

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microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis

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Targeting genomic SARS-CoV-2 RNA with siRNAs allows efficient inhibition of viral replication and spread

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Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery

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Current models of pulmonary fibrosis for future drug discovery efforts

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Human Lung Organoids—A Novel Experimental and Precision Medicine Approach

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Harnessing organs-on-a-chip to model tissue regeneration

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Lung repair and regeneration: Advanced models and insights into human disease

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Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

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Embedding of Precision-Cut Lung Slices in Engineered Hydrogel Biomaterials Supports Extended Ex Vivo Culture

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Targeting Alveolar Repair in Idiopathic Pulmonary Fibrosis

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The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices

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Pulmonary fibrosis: Emerging diagnostic and therapeutic strategies

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Lab-scale siRNA and mRNA LNP manufacturing by various microfluidic mixing techniques – an evaluation of particle properties and efficiency

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What have we learned from basic science studies on idiopathic pulmonary fibrosis?

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Human-Based Advanced in vitro Approaches to Investigate Lung Fibrosis and Pulmonary Effects of COVID-19

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The Antifibrotic Activity of Prostacyclin Receptor Agonism Is Mediated through Inhibition of YAP/TAZ

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Increased expression of OPN contributes to idiopathic pulmonary fibrosis and indicates a poor prognosis

Ji, J., Zheng, S., Liu, Y., Xie, T., Zhu, X., Nie, Y., Shen, Y., & Han, X. (2023) · Journal of Translational Medicine · doi:10.1186/s12967-023-04279-0 · Cited by 51

The evolution ofin vitromodels of lung fibrosis: promising prospects for drug discovery

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A model of human lung fibrogenesis for the assessment of anti-fibrotic strategies in idiopathic pulmonary fibrosis

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Perspectives on precision cut lung slices—powerful tools for investigation of mechanisms and therapeutic targets in lung diseases

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Dynamic expression of HOPX in alveolar epithelial cells reflects injury and repair during the progression of pulmonary fibrosis

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Transcriptional analysis of lung fibroblasts identifies PIM1 signaling as a driver of aging-associated persistent fibrosis

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Inhibition of SARS-CoV-2 replication in the lung with siRNA/VIPER polyplexes

Baldassi, D., Ambike, S. S., Feuerherd, M., Cheng, C., Peeler, D. J., Feldmann, D. P., Porras-Gonzalez, D., Wei, X. C., Keller, L.-A., Kneidinger, N., et al. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.03.051 · Cited by 47

Single Cell RNA-seq and Mass Cytometry Reveals a Novel and a Targetable Population of Macrophages in Idiopathic Pulmonary FibrosisPreprint

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Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

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Lung regeneration: implications of the diseased niche and ageing

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An integrated multiomic and quantitative label-free microscopy-based approach to study pro-fibrotic signalling in ex vivo human precision-cut lung slices

Khan, M. M., Poeckel, D., Halavatyi, A. A., Zukowska, J., Stein, F., Vappiani, J., Sévin, D. C., Tischer, C., Zinn, N., Eley, J. D., et al. (2020) · European Respiratory Journal · doi:10.1183/13993003.00221-2020 · Cited by 44

Update in Pulmonary Fibrosis 2018

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Precision-Cut Lung Slices: Emerging Tools for Preclinical and Translational Lung Research: An Official American Thoracic Society Workshop Report

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Transcriptomic characterization of culture-associated changes in murine and human precision-cut tissue slices

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Histological signatures map anti-fibrotic factors in mouse and human lungs

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GPCR-induced YAP activation sensitizes fibroblasts to profibrotic activity of TGFβ1

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Evaluation of Pirfenidone and Nintedanib in a Human Lung Model of Fibrogenesis

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Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics

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Precision-cut lung slices from bleomycin treated animals as a model for testing potential therapies for idiopathic pulmonary fibrosis

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Distinct niches within the extracellular matrix dictate fibroblast function in (cell free) 3D lung tissue cultures

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The Role of Pulmonary Surfactant Phospholipids in Fibrotic Lung Diseases

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Epithelial stem cells and niches in lung alveolar regeneration and diseases

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A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases

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Inhibition of epithelial cell YAP-TEAD/LOX signaling attenuates pulmonary fibrosis in preclinical models

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3D In Vitro Models: Novel Insights into Idiopathic Pulmonary Fibrosis Pathophysiology and Drug Screening

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Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices

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Modeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoids

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Klotho antagonizes pulmonary fibrosis through suppressing pulmonary fibroblasts activation, migration, and extracellular matrix production: a therapeutic implication for idiopathic pulmonary fibrosis

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Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures

Patel, V., Amin, K., Wahab, A., Marimoutou, M., Ukishima, L., Alvarez, J., Battle, K., Stucki, A., Clippinger, A. J., & Behrsing, H. P. (2023) · Toxicological Sciences · doi:10.1093/toxsci/kfac136 · Cited by 29

Tissue-informed engineering strategies for modeling human pulmonary diseases

Bailey, K. E., Floren, M. L., D’Ovidio, T. J., Lammers, S. R., Stenmark, K. R., & Magin, C. M. (2018) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00353.2018 · Cited by 32

Innovative three-dimensional models for understanding mechanisms underlying lung diseases: powerful tools for translational research

Nizamoglu, M., Joglekar, M. M., Almeida, C. R., Larsson‐Callerfelt, A., Dupin, I., Guenat, O. T., Henrot, P., van Os, L., Otero, J., Elowsson, L., et al. (2023) · European Respiratory Review · doi:10.1183/16000617.0042-2023 · Cited by 33

Where We Stand: Lung Organotypic Living Systems That Emulate Human-Relevant Host–Environment/Pathogen Interactions

Jiménez-Valdés, R. J., Can, U. I., Niemeyer, B. F., & Benam, K. H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00989 · Cited by 27

Classical and Non-classical Fibrosis Phenotypes Are Revealed by Lung and Cardiac Like Microvascular Tissues On-Chip

Akinbote, A., Beltrán-Sastre, V., Cherubini, M., Visone, R., Hajal, C., Cobanoglu, D., & Haase, K. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.735915 · Cited by 25

Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos

Gvazava, N., Konings, S. C., Cepeda-Prado, E., Skoryk, V. R., Umeano, C. H., Dong, J., Silva, I. A. N., Ottosson, D. R., Leigh, N. D., Wagner, D. E., et al. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.3c08854 · Cited by 26

Stem cell migration drives lung repair in living mice

Chioccioli, M., Liu, S., Magruder, S., Tata, A., Borriello, L., McDonough, J. E., Konkimalla, A., Kim, S., Nouws, J., Gonzalez, D. G., et al. (2024) · Developmental Cell · doi:10.1016/j.devcel.2024.02.003 · Cited by 26

The Use of Precision‐Cut Lung Slices for Studying Innate Immunity to Viral Infections

Michalaki, C., Dean, C. H., & Johansson, C. (2022) · Current Protocols · doi:10.1002/cpz1.505 · Cited by 26

Modeling Pneumonic Plague in Human Precision-Cut Lung Slices Highlights a Role for the Plasminogen Activator Protease in Facilitating Type 3 Secretion

Banerjee, S. K., Huckuntod, S. D., Mills, S. D., Kurten, R. C., & Pechous, R. D. (2019) · Infection and Immunity · doi:10.1128/iai.00175-19 · Cited by 25

Fatty acid nitroalkene reversal of established lung fibrosis

Koudelka, A., Cechova, V., Rojas, M. M., Mitash, N., Bondonese, A., Croix, C. M. S., Ross, M. A., & Freeman, B. Α. (2021) · Redox Biology · doi:10.1016/j.redox.2021.102226 · Cited by 28

Three-dimensional models of the lung: past, present and future: a mini review

Sen, C., Freund, D., & Gomperts, B. N. (2022) · Biochemical Society Transactions · doi:10.1042/bst20190569 · Cited by 25

Molecular characterization of a precision-cut rat liver slice model for the evaluation of antifibrotic compounds

Huang, X., Cai, H., Ammar, R., Zhang, Y., Wang, Y., Ravi, K., Thompson, J. R., & Járai, G. (2018) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00281.2018 · Cited by 24

Extracellular Matrix as a Driver of Chronic Lung Diseases

Burgess, J. K., Weiss, D. J., Westergren‐Thorsson, G., Wigén, J., Dean, C. H., Mumby, S. E., Bush, A., & Adcock, I. M. (2024) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2023-0176ps · Cited by 31

Self‐Assembled Human Skin Equivalents Model Macrophage Activation of Cutaneous Fibrogenesis in Systemic Sclerosis

Huang, M., Smith, A., Watson, M. C., Bhandari, R., Baugh, L. M., Ivanovska, I. L., Watkins, T., Lang, I. M., Trojanowska, M. E., Black, L. D., et al. (2022) · Arthritis & Rheumatology · doi:10.1002/art.42097 · Cited by 23

Comparison of Long-term Human Precision-cut Lung Slice Culture Methodology and Response to Challenge: An Argument for Standardisation

Patel, V., Amin, K., Allen, D., Ukishima, L., Wahab, A., Grodi, C., & Behrsing, H. P. (2021) · Alternatives to Laboratory Animals · doi:10.1177/02611929211061884 · Cited by 22

Application of Precision-Cut Lung Slices as an In Vitro Model for Research of Inflammatory Respiratory Diseases

Liu, Y., Wu, P., Wang, Y., Liu, Y., Yang, H., Zhou, G., Wu, X., & Wen, Q. (2022) · Bioengineering · doi:10.3390/bioengineering9120767 · Cited by 22

The Activation of the RIG-I/MDA5 Signaling Pathway upon Influenza D Virus Infection Impairs the Pulmonary Proinflammatory Response Triggered by Mycoplasma bovis Superinfection

Gaudino, M., Lion, A., Sagné, E., Nagamine, B. S., Oliva, J., Terrier, O., Errazuriz-Cerda, E., Scribe, A., Sikht, F., Simon, E. D. S., et al. (2023) · Journal of Virology · doi:10.1128/jvi.01423-22 · Cited by 22

Alveolar repair following LPS-induced injury requires cell-ECM interactions

Sucre, J. M. S., Bock, F. M., Negretti, N. M., Benjamin, J. T., Gulleman, P. M., Dong, X., Ferguson, K. T., Jetter, C. S., Han, W., Liu, Y., et al. (2023) · JCI Insight · doi:10.1172/jci.insight.167211 · Cited by 23

Mouse Lung Tissue Slice Culture

Wu, X., van Dijk, E. M., Bos, I. S. T., Kistemaker, L. E. M., & Gosens, R. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9086-3_21 · Cited by 22

Albendazole ameliorates aerobic glycolysis in myofibroblasts to reverse pulmonary fibrosis

Zeng, C., Yue, H., Wang, C., Ju, X., Wang, T., Fu, X., Zhou, Q., Zhang, H., He, L., Yu, J., et al. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05655-0 · Cited by 21

Promises and Challenges of Cell-Based Therapies to Promote Lung Regeneration in Idiopathic Pulmonary Fibrosis

Egea-Zorrilla, A., Vera, L., Sáez, B., & Pardo–Saganta, A. (2022) · Cells · doi:10.3390/cells11162595 · Cited by 21

Human lung virtual histology by multi-scale x-ray phase-contrast computed tomography

Reichmann, J., Verleden, S. E., Kühnel, M., Kamp, J. C., Werlein, C., Neubert, L., Müller, J.-H., Bui, T. Q., Ackermann, M., Jonigk, D. D., et al. (2023) · Physics in Medicine and Biology · doi:10.1088/1361-6560/acd48d · Cited by 21

Co-culture of human AT2 cells with fibroblasts reveals a MUC5B phenotype: insights from an organoid model

Yao, Y., Ritzmann, F., Miethe, S., Kattler‐Lackes, K., Colakoglu, B., Herr, C., Kamyschnikow, A., Brand, M., Garn, H., Yildiz, D., et al. (2024) · Molecular Medicine · doi:10.1186/s10020-024-00990-w · Cited by 20

IL-4-induced SOX9 confers lineage plasticity to aged adult lung stem cells

Cai, X. T., Jia, M., Heigl, T., Shamir, E. R., Wong, A. K., Hall, B. M., Arlantico, A., Hung, J., Menon, H. G., Darmanis, S., et al. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114569 · Cited by 20

Biofabrication: An interesting tool to create in vitro model for COVID-19 drug targets

Singh, A. K., Mishra, G., Maurya, A., Kulkarni, G. T., & Awasthi, R. (2020) · Medical Hypotheses · doi:10.1016/j.mehy.2020.110059 · Cited by 19

Molecular characterization of a precision-cut rat lung slice model for the evaluation of antifibrotic drugs

Huang, X., Li, L., Ammar, R., Zhang, Y., Wang, Y., Ravi, K., Thompson, J. R., & Járai, G. (2018) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00339.2018 · Cited by 19

Transcriptomic profiling of induced steatosis in human and mouse precision-cut liver slices

Simon, E., Motyka, M., Prins, G. H., Li, M., Rust, W., Kauschke, S. G., Viollet, C., Olinga, P., & Oldenburger, A. (2023) · Scientific Data · doi:10.1038/s41597-023-02220-0 · Cited by 19

Dehydroepiandrosterone in fibrotic interstitial lung disease: a translational study

Guler, S. A., Machahua, C., Geiser, T., Kocher, G. J., Marti, T. M., Tan, B., Trappetti, V., Ryerson, C. J., & Funke-Chambour, M. (2022) · Respiratory Research · doi:10.1186/s12931-022-02076-9 · Cited by 17

Pulmonary Fibrosis Stakeholder Summit: A Joint NHLBI, Three Lakes Foundation, and Pulmonary Fibrosis Foundation Workshop Report

Montesi, S. B., Gómez, C. R., Beers, M. F., Brown, R., Chattopadhyay, I., Flaherty, K. R., Garcia, C. K., Gomperts, B. N., Hariri, L. P., Hogaboam, C. M., et al. (2023) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.202307-1154ws · Cited by 21

Identification of Paired-related Homeobox Protein 1 as a key mesenchymal transcription factor in pulmonary fibrosis

Marchal‐Duval, E., Homps-Legrand, M., Froidure, A., Jaillet, M., Ghanem, M., Deneuville, L., Justet, A., Maurac, A., Vadel, A., Fortas, E., et al. (2023) · eLife · doi:10.7554/elife.79840 · Cited by 20

Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices

Ram‐Mohan, S., Bai, Y., Schaible, N., Ehrlicher, A. J., Cook, D. P., Suki, B., Stoltz, D. A., Solway, J., Ai, X., & Krishnan, R. (2019) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00297.2019 · Cited by 18

Characterization of Human Precision Cut Lung Slices as an ex vivo model for Idiopathic Pulmonary Fibrosis

Marchini, G., Volpato, F., Spagnoli, B., & Villetti, G. (2025) · doi:10.1183/13993003.congress-2025.pa6086

Ex vivo modeling of human lung fibrogenesis and drug mode of action screens using single cell RNA-seq in precision-cut lung slices

Lang, N. J., Ansari, M., Yang, L., Zhou, S., Porras-Gonzalez, D., Agami, A., Mayr, C. H., Kashani, B. H., Heumos, L., Gerckens, M., et al. (2022) · doi:10.1183/13993003.congress-2022.1851

Human precision-cut lung slices generated from excess donor lungs as a model for IPF and drug screening

Stegmayr, J., Alsafadi, H. N., Lindstedt, S., & Wagner, D. E. (2021) · 12.01 - Idiopathic interstitial pneumonias · doi:10.1183/23120541.lsc-2021.106

Ex vivo modelling of human lung fibrogenesis and drug mode of action screens using single-cell RNA-seq in precision-cut lung slices

Lang, N. J., Ansari, M., Porras-Gonzalez, D., Agami, A., Kashani, B. H., Zhou, S., Yang, L., Gerckens, M., Wei, X., Mayr, C. H., et al. (2022) · doi:10.1183/23120541.lsc-2022.72

Late Breaking Abstract - PCLS as a human, in vitro IPF model – morphological and molecular insights

Scheer, S., Tielemans, B., Riese, J., Dietrich, J., Rattonitz, S. V. S. U., Krüger, I., Alhussein, K., Martin, C., Schlüter, H., Neubert, L., et al. (2025) · doi:10.1183/13993003.congress-2025.pa1902