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The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia
Open accessAbstract
Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide expression profile of tracheal and bronchial human airway epithelia in vivo and compared it with the expression profile of primary cultures of human airway epithelia grown at the air-liquid interface. For comparison, we also investigated the expression profile of Calu-3 cells grown at the air-liquid interface and primary cultures of human airway epithelia submerged in nutrient media. We found that the transcriptional profile of differentiated primary cultures grown at the air-liquid interface most closely resembles that of in vivo airway epithelia, suggesting that the use of primary cultures and the presence of an air-liquid interface are important to recapitulate airway epithelia biology. We describe a high level of similarity between cells of tracheal and bronchial origin within and between different human donors, which suggests a very robust expression profile that is specific to airway cells.
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Summaries of Affymetrix GeneChip probe level data
Irizarry, R. A. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng015 · Cited by 4,887
GOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene lists
Eden, E., Navon, R., Steinfeld, I., Lipson, D., & Yakhini, Z. (2009) · BMC Bioinformatics · doi:10.1186/1471-2105-10-48 · Cited by 3,356
Reich, M., Liefeld, T., Gould, J., Lerner, J., Tamayo, P., & Mesirov, J. P. (2006) · Nature Genetics · doi:10.1038/ng0506-500 · Cited by 2,032
Discovering motifs in ranked lists of DNA sequences
Eden, E., Lipson, D., Yogev, S., & Yakhini, Z. (2007) · PLoS Computational Biology · doi:10.1371/journal.pcbi.0030039 · Cited by 696
Cell number and distribution in human and rat airways.
Mercer, R. R., Russell, M. L., Roggli, V. L., & Crapo, J. D. (1994) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.10.6.8003339 · Cited by 395
Differentiated structure and function of cultures from human tracheal epithelium
Yamaya, M., Finkbeiner, W. E., Chun, S. Y., & Widdicombe, J. H. (1992) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1992.262.6.l713 · Cited by 361
Karp, P. H., Moninger, T. O., Weber, S. P., Nesselhauf, T. S., Launspach, J. L., Zabner, J., & Welsh, M. J. (2003) · Epithelial Cell Culture Protocols · doi:10.1385/1-59259-185-x:115 · Cited by 342
Self-citationWhitcutt, M. J., Adler, K. B., & Wu, R. (1988) · In Vitro Cellular & Developmental Biology · doi:10.1007/bf02628493 · Cited by 254
Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution
Brunskill, E. W., Aronow, B. J., Georgas, K., Rumballe, B., Valerius, M. T., Aronow, J., Kaimal, V., Jegga, A. G., Grimmond, S., McMahon, A. P., et al. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.09.007 · Cited by 216
Modification of gene expression of the small airway epithelium in response to cigarette smoking
Harvey, B.-G., Heguy, A., Leopold, P. L., Carolan, B. J., Ferris, B., & Crystal, R. G. (2006) · Journal of Molecular Medicine · doi:10.1007/s00109-006-0103-z · Cited by 181
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Rezania, A., Bruin, J. E., Arora, P., Rubin, A., Batushansky, I., Asadi, A., O'Dwyer, S., Quiskamp, N., Mojibian, M., Albrecht, T., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.3033 · Cited by 1,575
The innate immune function of airway epithelial cells in inflammatory lung disease
Hiemstra, P. S., McCray, P. B., & Bals, R. (2015) · European Respiratory Journal · doi:10.1183/09031936.00141514 · Cited by 422
Self-citationKeeley, T. P., & Mann, G. E. (2019) · Physiological Reviews · doi:10.1152/physrev.00041.2017 · Cited by 329
Benam, K. H., Novak, R., Nawroth, J., Hirano-Kobayashi, M., Ferrante, T. C., Choe, Y., Prantil-Baun, R., Weaver, J. C., Bahinski, A., Parker, K. K., et al. (2016) · Cell Systems · doi:10.1016/j.cels.2016.10.003 · Cited by 309
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Mahaddalkar, P. U., Scheibner, K., Pfluger, S., Ansarullah, Sterr, M., Beckenbauer, J., Irmler, M., Beckers, J., Knöbel, S., & Lickert, H. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0492-5 · Cited by 115
Metadata
- Authors
- Pezzulo, A. A., Starner, T. D., Scheetz, T. E., Traver, G. L., Tilley, A. E., Harvey, B.-G., Crystal, R. G., McCray, P. B., & Zabner, J.
- Venue
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Publisher
- American Physiological Society
- Published
- 2011-01
- Volume
- 300
- Issue
- 1
- Pages
- L25-L31
- ISSN
- 1040-0605 (ISSN-L)
- 1522-1504
- OpenAlex
- W2024990391
- Cited by
- 388
- References
- 20
- FWCI
- 8.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Pezzulo, A. A., Starner, T. D., Scheetz, T. E., Traver, G. L., Tilley, A. E., Harvey, B.-G., Crystal, R. G., McCray, P. B., & Zabner, J. (2011). The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia. American Journal of Physiology-Lung Cellular and Molecular Physiology. https://doi.org/10.1152/ajplung.00256.2010
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