The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Stretch induces cytokine release by alveolar epithelial cells in vitro
Abstract
Mechanical ventilation can injure the lung, causing edema and alveolar inflammation. Interleukin-8 (IL-8) plays an important role in this inflammatory response. We postulated that cyclic cell stretch upregulates the production and release of IL-8 by human alveolar epithelium in the absence of structural cell damage or paracrine stimulation. To test this hypothesis, alveolar epithelial cells (A549 cells) were cultured on a deformable silicoelastic membrane. When stretched by 30% for up to 48 h, the cells released 49 ± 34% more IL-8 ( P < 0.001) than static controls. Smaller deformations (20% stretch) produced no consistent increase in IL-8. Stretch of 4 h duration increased IL-8 gene transcription fourfold above baseline. Stretch had no effect on cell proliferation, cell viability as assessed by 51 Cr release assay, or the release of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-α. We conclude that deformation per se can trigger inflammatory signaling and that alveolar epithelial cells may be active participants in the alveolitis associated with ventilator-induced lung injury.
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Cites
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Cited by
Alveolar epithelial type II cell: defender of the alveolus revisited
Fehrenbach, H. (2001) · Respiratory Research · doi:10.1186/rr36 · Cited by 757
Cytokine-mediated inflammation in acute lung injury
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Douville, N. J., Zamankhan, P., Tung, Y.-C., Li, R., Vaughan, B. L., Tai, C.-F., White, J., Christensen, P. J., Grotberg, J. B., & Takayama, S. (2011) · Lab Chip · doi:10.1039/c0lc00251h · Cited by 216
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Metadata
- Authors
- Vlahakis, N. E., Schroeder, M. A., Limper, A. H., & Hubmayr, R. D.
- Venue
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Publisher
- American Physiological Society
- Published
- 1999-07-01
- Volume
- 277
- Issue
- 1
- Pages
- L167-L173
- ISSN
- 1040-0605 (ISSN-L)
- 1522-1504
- OpenAlex
- W2227906064
- Cited by
- 389
- References
- 24
- FWCI
- 13.94
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vlahakis, N. E., Schroeder, M. A., Limper, A. H., & Hubmayr, R. D. (1999). Stretch induces cytokine release by alveolar epithelial cells in vitro. American Journal of Physiology-Lung Cellular and Molecular Physiology. https://doi.org/10.1152/ajplung.1999.277.1.l167
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