Mechanical Stretch Stimulates Interleukin-8 Production in Endometrial Stromal Cells: Possible Implications in Endometrium-Related Events
Abstract
Uterine movement is suggested to play roles in various events related to the uterus. In view of the current concept underscoring the biological implications of mechanical stretch, we speculated that the mechanical stretch exerted by uterine movement might stimulate the production of biochemical mediators in endometrial cells and contribute to inflammation-associating processes, such as menstruation and endometriosis. To address the possible effects of mechanical stretch in the endometrium, endometrial stromal cells (ESC) were cultured on flexible-bottomed culture plates, and cyclic stretch (25% elongation) was applied in serum-free conditions at a rate of two cycles per minute using a computer-operated cell tension system. IL-8 concentrations in the conditioned medium were measured using ELISA, and IL-8 mRNA expression in ESC was measured by RT-PCR. Cyclic stretch increased the secretion of IL-8 from ESC. The increase in IL-8 secretion was inhibited by PD98059, an inhibitor of extracellular signal-regulated kinase 1/2. The increase was also inhibited by progesterone. In addition, the conditioned medium of ESC cultured with cyclic stretch stimulated the mRNA expression of IL-8 in ESC cultured under stationary conditions. These findings imply that uterine movement has an impact on endometrium-related physiology and pathology by stimulating the production of a biochemical mediator(s) in the endometrium.
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Expression of acute and inflammatory cytokines such as interleukin-1β and interleukin 6 and also interleukin-8 facilitate implantation.
“The expression of acute and inflammatory cytokines such as interleukin-1β and interleukin 6 and also interleukin-8 [65, 74] facilitate implantation.”
From The pathophysiology of endometriosis and adenomyosis, tissue injury and repair (2009) · cites this paper as [65]
Even minor increments in mechanical strain result in activation of COX-2 and production of PGE2, the basic biochemical mechanisms underlying tissue injury, and also in production of interleukin-8.
“However, even minor increments in mechanical strain resulted and the activation of COX-2 and the production of PGE2, the basic biochemical mechanisms underlying tissue injury [54], and also in the production of interleukin-8 [65].”
From The pathophysiology of endometriosis and adenomyosis, tissue injury and repair (2009) · cites this paper as [65]
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The pathophysiology of endometriosis and adenomyosis, tissue injury and repair
Leyendecker, G., Wildt, L., & Mall, G. (2009) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-009-1191-0 · Cited by 418 · see citing works
“The expression of acute and inXammatory cytokines such as interleukin-1 and interleukin 6 and also interleukin-8 [65, 74] facilitate implantation.”
BackgroundA new concept of endometriosis and adenomyosis, tissue injury and repair (TIAR)
Leyendecker, G., & Wildt, L. (2011) · hmbci · doi:10.1515/hmbci.2011.002 · Cited by 147 · see citing works
An endometriotic vault fistula presenting with monthly bleeding after hysterectomy
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- Authors
- Harada, M., Osuga, Y., Hirota, Y., Koga, K., Morimoto, C., Hirata, T., Yoshino, O., Tsutsumi, O., Yano, T., & Taketani, Y.
- Affiliations
- The University of Tokyo (JP)
- Corresponding author
- Osuga, Yutaka
- ORCID
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2005-02
- Volume
- 90
- Issue
- 2
- Pages
- 1144-1148
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2030523774
- Cited by
- 48
- References
- 31
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 6 · 2020: 1 · 2019: 2 · 2017: 1 · 2015: 1 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 1.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- mechanical stretch, interleukin-8, endometrial stromal cells, cyclic strain, endometriosis, menstruation, inflammation, ERK signaling, progesterone, endometrium
- MeSH terms
- Adult, Base Sequence, Endometrium, Enzyme Inhibitors, Estradiol, Female, Flavonoids, Gene Expression Regulation, Humans, Menstruation, Progesterone, RNA, Messenger, Stress, Mechanical, Interleukin-8, Stromal Cells, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction
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