Cited by 15Biochemical Analysis and Sensing TechniquesRegulation of Appetite and ObesityLipid metabolism and biosynthesis

Mice bearing Acads mutation display altered postingestive but not 5-s orosensory response to dietary fat

Smith Richards, Brenda K., Belton, Brenda N., York, Barbara, Volaufova, Julia·2004·American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Abstract

A previous survey of mouse inbred strains revealed a wide range in self-selected fat intake, from 26 to 83% of energy. The BALB/cByJ strain selected a lower percentage of fat intake (36%) than all other strains tested except for the CAST/Ei. BALB/cByJ mice are deficient in the short-chain acyl-CoA dehydrogenase (SCAD) enzyme due to a spontaneous mutation in Acads. We hypothesized that this deficiency would alter fat appetite and used three behavioral test paradigms to compare the response of BALB/cByKz. Acads -/- and BALB/cByKz. Acads +/+ mice to fat stimuli. First, during 10-day exposure to a macronutrient self-selection diet, Acads -/- mice consumed proportionately less fat and more carbohydrate than Acads +/+ mice, yet total energy intake was similar between strains. Next, in 48-h two-bottle preference tests, Acads +/+ mice displayed a preference for 50% corn oil, but Acads -/- mice did not. Finally, in brief-access taste tests employing successive 5-s presentations of corn oil in an ascending concentration series ending with 50%, there were no effects of strain on total licks, indicating that Acads does not alter acute orosensory response to this fat stimulus. With 15-s presentations, however, the Acads +/+ mice licked more of the 50% oil than Acads -/-, suggesting orosensory effects related to the increased exposure time. In contrast to corn oil, there were no strain differences in licking response to sucrose solution in either the two-bottle or brief-access taste tests. The observation that SCAD-deficient mice display altered postingestive responses to dietary fat provides further evidence for the metabolic control of feeding.

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Crossref · 10.1152/ajpregu.00488.2003

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Authors
Smith Richards, B. K., Belton, B. N., York, B., & Volaufova, J.
Affiliations
Pennington Biomedical Research Center (US)
Venue
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Published
2004-02
Volume
286
Issue
2
Pages
R311-R319
ISSN
  • 0363-6119 (ISSN-L)
  • 1522-1490
OpenAlex
W2123801449
Cited by
15
References
55
Citations by year
2022: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
0.90
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK 53113, R01DK053113)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
short-chain acyl-CoA dehydrogenase deficiency, dietary fat intake, corn oil, sucrose, BALB/cByJ mice, inbred mouse strains
MeSH terms
Animals, Corn Oil, Diet, Dietary Fats, Drinking Behavior, Feeding Behavior, Food Preferences, Mice, Inbred BALB C, Mouth, Mutation, Osmolar Concentration, Sensation, Sucrose, Time Factors, Gene Deletion, Butyryl-CoA Dehydrogenase, Mice

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