Mice bearing Acads mutation display altered postingestive but not 5-s orosensory response to dietary fat
Abstract
A previous survey of mouse inbred strains revealed a wide range in self-selected fat intake, from 26 to 83% of energy. The BALB/cByJ strain selected a lower percentage of fat intake (36%) than all other strains tested except for the CAST/Ei. BALB/cByJ mice are deficient in the short-chain acyl-CoA dehydrogenase (SCAD) enzyme due to a spontaneous mutation in Acads. We hypothesized that this deficiency would alter fat appetite and used three behavioral test paradigms to compare the response of BALB/cByKz. Acads -/- and BALB/cByKz. Acads +/+ mice to fat stimuli. First, during 10-day exposure to a macronutrient self-selection diet, Acads -/- mice consumed proportionately less fat and more carbohydrate than Acads +/+ mice, yet total energy intake was similar between strains. Next, in 48-h two-bottle preference tests, Acads +/+ mice displayed a preference for 50% corn oil, but Acads -/- mice did not. Finally, in brief-access taste tests employing successive 5-s presentations of corn oil in an ascending concentration series ending with 50%, there were no effects of strain on total licks, indicating that Acads does not alter acute orosensory response to this fat stimulus. With 15-s presentations, however, the Acads +/+ mice licked more of the 50% oil than Acads -/-, suggesting orosensory effects related to the increased exposure time. In contrast to corn oil, there were no strain differences in licking response to sucrose solution in either the two-bottle or brief-access taste tests. The observation that SCAD-deficient mice display altered postingestive responses to dietary fat provides further evidence for the metabolic control of feeding.
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Animals will avoid fat intake in states where fat metabolism is defective or blocked.
“there is evidence that animals will avoid fat intake in states where fat metabolism is defective/blocked”
From FGF21 and the physiological regulation of macronutrient preference (2020) · cites this paper as [49]
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- Authors
- Smith Richards, B. K., Belton, B. N., York, B., & Volaufova, J.
- Affiliations
- Pennington Biomedical Research Center (US)
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 2004-02
- Volume
- 286
- Issue
- 2
- Pages
- R311-R319
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2123801449
- Cited by
- 15
- References
- 55
- Citations by year
- 2022: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 53113, R01DK053113)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- short-chain acyl-CoA dehydrogenase deficiency, dietary fat intake, corn oil, sucrose, BALB/cByJ mice, inbred mouse strains
- MeSH terms
- Animals, Corn Oil, Diet, Dietary Fats, Drinking Behavior, Feeding Behavior, Food Preferences, Mice, Inbred BALB C, Mouth, Mutation, Osmolar Concentration, Sensation, Sucrose, Time Factors, Gene Deletion, Butyryl-CoA Dehydrogenase, Mice
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