The DOI matches its Crossref record.
Interleukin 1 alters rat sleep: temporal and dose-related effects
Abstract
Rats received various doses of interleukin 1 (IL-1) (range, 0.5-25.0 ng) or pyrogen-free saline intracerebroventricularly during the rest (light) and the active (dark) cycles of the day, and sleep-wake activity and brain temperature were determined for 6 h. Low doses of IL-1 (0.5 ng at night, 2.5 ng during the day) increased both the duration of non-rapid-eye-movement sleep (NREMS) and electroencephalogram (EEG) slow-wave activity during NREMS episodes. Increasing doses of IL-1 had divergent effects on NREMS duration and EEG slow-wave activity, and the direction of the changes depended on the diurnal cycle. Thus NREMS duration was promoted at night and EEG slow-wave amplitudes during the day, whereas NREMS duration during the day and EEG slow-wave amplitudes at night were suppressed after higher doses of IL-1. High doses of IL-1 also induced decreases in rapid-eye-movement sleep during both phases of the day. Each dose of IL-1 that promoted NREMS also tended to increase brain temperature. These results demonstrate that IL-1 promotes NREMS in the rat. However, unlike previously reported findings in rabbits, the circadian rhythm of sleep regulation strongly interferes with the sleep-promoting activity of IL-1 in rats.
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In the citing paper
“These findings established both cytokines, IL-1 and TNF, as substances involved in the homeostatic regulation of sleep (for review, see Refs. 299, 411).”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [411]
In the citing paper
“increasing the availability of those cytokines promoted NREM sleep amount and intensity and suppressed REM sleep amount.”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [411]
In the citing paper
“blocking the biological actions of the cytokines IL-1 and TNF resulted in a reduction of physiological NREM sleep amount or NREM sleep rebound after sleep deprivation.”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [411]
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“IL-1 administration has been associated with sleep induction when administered intracerebroventricularly, and anti-IL-1 β injection has subsequently reduced sleep (Opp and Krueger, 1994; Opp et al., 1991).”
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Zielinski, M. R., & Gibbons, A. J. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822963-7.00312-1 · Cited by 1
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Ingiosi, A. M. (2015) · Deep Blue (University of Michigan)
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Chaskiel, L., & Konsman, J. P. (2008) · Neuroimmune biology · doi:10.1016/s1567-7443(07)10004-1
Krueger, J. M. (1998) · Journal of Musculoskeletal Pain · doi:10.1300/j094v06n03_16
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Wang, F., Zhang, Z., Xia, L., Shi, C.-Y., & Chen, G.-H. (2023) · Research Square · doi:10.21203/rs.3.rs-3191107/v1
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“Administration of IL-1 confirms that brain IL-1 affects neurophysiological functions including sleep (Opp et al., 1991), body temperature regulation (Blatteis and Sehic, 1998), hypothalamus-pituitary-adrenal axis activity (Goshen and Yirmiya,2009), food and water intake (Liu et al., 2019),…”
Metadata
- Authors
- Opp, M. R., Obal, F., & Krueger, J. M.
- Affiliations
- University of Tennessee Health Science Center (US)
- Department of Physiology and Biophysics, University of Tennessee,Memphis 38163.
- ORCID
- Opp, M. R.
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 1991-01-01
- Volume
- 260
- Issue
- 1
- Pages
- R52-R58
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2286922506
- Cited by
- 232
- References
- 0
- Citations by year
- 2026: 1 · 2025: 11 · 2024: 5 · 2023: 5 · 2022: 5 · 2021: 7 · 2020: 8 · 2019: 6 · 2018: 1 · 2017: 6 · 2016: 5 · 2015: 6 · 2014: 3 · 2013: 7 · 2012: 7
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS-27250, NS-25378, R01NS027250, R01NS025378)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroscience of respiration and sleep
- Keywords
- interleukin-1, sleep regulation, NREM sleep, REM sleep, EEG slow-wave activity, circadian rhythm, dose-dependent effects, brain temperature, intracerebroventricular administration, rats
- MeSH terms
- Animals, Brain, Circadian Rhythm, Dose-Response Relationship, Drug, Electroencephalography, Interleukin-1, Light, Male, Rats, Inbred Strains, Sleep, REM, Temperature, Rats
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