HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise

Abstract

To examine the effect of exercise on heat shock protein (HSP) 72 mRNA expression in skeletal muscle, five healthy humans (20 ± 1 yr; 64 ± 3 kg; peak O 2 uptake of 2.55 ± 0.2 l/min) cycled until exhaustion at a workload corresponding to 63% peak O 2 uptake. Muscle was sampled from the vastus lateralis, and muscle temperature was measured at rest (R), 10 min of exercise (Min10), ∼40 min before fatigue (F-40 = 144 ± 7 min), and fatigue (F = 186 ± 15 min). Muscle samples were analyzed for HSP72 mRNA expression, as well as glycogen and lactate concentration. Muscle temperature increased ( P < 0.05) during the first 10 min of exercise but then remained constant for the duration of the exercise. Similarly, lactate concentration increased ( P < 0.05) when Min10 was compared with R but decreased ( P < 0.05) thereafter, such that concentrations at F-40 and F were not different from those at R. In contrast, muscle glycogen concentration fell progressively throughout exercise (486 ± 74 vs. 25 ± 7 mmol/kg dry weight for R and F, respectively; P < 0.05). HSP72 mRNA was detected at R but did not increase by Min10. However, HSP72 mRNA increased ( P < 0.05) 2.2 ± 0.5- and 2.6 ± 0.9-fold, respectively, when F-40 and F were compared with R. These data demonstrate that HSP72 mRNA increases progressively during acute cycling, suggesting that processes that take place throughout concentric exercise are capable of initiating a stress response.