Heterogeneity of electrically evoked dopamine release and reuptake in substantia nigra, ventral tegmental area, and striatum
Abstract
Somatodendritic dopamine (DA) released in substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA) may mediate extrasynaptic neuronal signaling. The concentration of extracellular DA ([DA]o) attained during somatodendritic activation will be governed by the density of release sites and properties of DA uptake. We evaluated these factors in SNc, VTA, and dorsal striatum with carbon-fiber microelectrodes and fast-scan cyclic voltammetry to monitor [DA]o during local electrical stimulation (10 Hz, 5 s) in guinea pig brain slices. Stimulated DA efflux was site specific, with significantly higher [DA]o in caudal (0.48 +/- 0.03 microM, mean +/- SE) than rostral SNc (0.16 +/- 0.01 microM), averaged over their mediolateral extents, and higher [DA]o in VTA (0.74 +/- 0.07 microM) than in medial (0.43 +/- 0.04 microM) or lateral SNc (0.29 +/- 0.05 microM), averaged rostrocaudally. Throughout SNc, evoked [DA]o correlated positively (r = 0.91) with the density of tyrosine-hydroxylase-immunoreactive cells. Modulation of evoked [DA]o by uptake was also site specific. The selective DA uptake inhibitor GBR 12909 significantly increased evoked [DA]o in caudal SNc (to 185 +/- 27%) and striatum (408 +/- 24%), but had no effect in rostral SNc or VTA. Conversely, the norepinephrine (NE) uptake inhibitor desipramine did not alter stimulated [DA]o in caudal SNc or striatum, but caused significant enhancement in rostral SNc (196 +/- 17%) and VTA (126 +/- 12%). Paroxetine, a selective…
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Within the striatum, dopamine re-uptake is exceptionally fast.
“Within the striatum, dopamine re-uptake is exceptionally fast (e.g., [71]).”
From Cortical and basal ganglia contributions to habit learning and automaticity (2010) · cites this paper as [71]
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- Authors
- Cragg, S. J., Rice, M. E., & Greenfield, S. A.
- Affiliations
- University of Oxford (GB)
- Venue
- Journal of Neurophysiology
- Publisher
- American Physiological Society
- Published
- 1997-02-01
- Volume
- 77
- Issue
- 2
- Pages
- 863-873
- ISSN
- 0022-3077 (ISSN-L)
- 1522-1598
- OpenAlex
- W2196775293
- Cited by
- 118
- References
- 78
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 7 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 8
- FWCI
- 2.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurophysiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Keywords
- dopamine reuptake, substantia nigra pars compacta, ventral tegmental area, dorsal striatum, tyrosine hydroxylase, dopamine beta-hydroxylase, GBR 12909, desipramine, paroxetine, norepinephrine uptake, serotonin uptake
- MeSH terms
- Animals, Corpus Striatum, Dopamine, Electric Stimulation, Guinea Pigs, Male, Substantia Nigra, Tegmentum Mesencephali
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