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Cited by 42Ion channel regulation and functionNeuroscience and Neuropharmacology ResearchReceptor Mechanisms and Signaling

Muscarine Modulates Ca 2+ Channel Currents in Rat Sensorimotor Pyramidal Cells Via Two Distinct Pathways

Stewart, Ansalan E., Yan, Zhen, Surmeier, D. James, Foehring, Robert C.·1999·Journal of Neurophysiology
DOI 10.1152/jn.1999.81.1.72
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Abstract

Stewart, Ansalan E., Zhen Yan, D. James Surmeier, and Robert C. Foehring. Muscarine modulates Ca 2+ channel currents in rat sensorimotor pyramidal cells via two distinct pathways. J. Neurophysiol. 81: 72–84, 1999. We used the whole cell patch-clamp technique and single-cell reverse transcription-polymerase chain reaction (RT-PCR) to study the muscarinic receptor-mediated modulation of calcium channel currents in both acutely isolated and cultured pyramidal neurons from rat sensorimotor cortex. Single-cell RT-PCR profiling for muscarinic receptor mRNAs revealed the expression of m1, m2, m3, and m4 subtypes in these cells. Muscarine reversibly reduced Ca 2+ currents in a dose-dependent manner. The modulation was blocked by the muscarinic antagonist atropine. When the internal recording solution included 10 mM ethylene glycol-bis(β-aminoethyl ether)- N,N,N′,N′-tetraacetic acid (EGTA) or 10 mM bis-( o-aminophenoxy)- N,N,N′,N′-tetraacetic acid (BAPTA), the modulation was rapid (τ onset ∼ 1.2 s). Under conditions where intracellular calcium levels were less controlled (0.0–0.1 mM BAPTA), a slowly developing component of the modulation also was observed (τ onset ∼17 s). Both fast and slow components also were observed in recordings with 10 mM EGTA or 20 mM BAPTA when Ca 2+ was added to elevate internal [Ca 2+ ] (∼150 nM). The fast component was due to a reduction in both N- and P-type calcium currents, whereas the slow component involved L-type current. N-ethylmaleimide blocked the fast component but not the slow component of the modulation. Preincubation of cultured neurons with pertussis toxin (PTX) also greatly reduced the fast portion of the modulation. These results suggest a role for both PTX-sensitive G proteins as well as PTX-insensitive G proteins in the muscarinic modulation. The fast component of the modulation was reversed by strong depolarization, whereas the slow component was not. Reblock of the calcium channels by G proteins (at −90 mV) occurred with a median τ of 68 ms. We conclude that activation of muscarinic receptors results in modulation of N- and P-type channels by a rapid, voltage-dependent pathway and of L-type current by a slow, voltage-independent pathway.

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  • Activation of mAChRs on cortical pyramidal neurons reduces the activity of multiple types of Ca2+ channels.

    “Activation of mAChRs on cortical pyramidal neurons reduces the activity of multiple types of Ca2+ channels176”

    From Mechanisms underlying gain modulation in the cortex (2020) · cites this paper as [176]

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Authors
Stewart, A. E., Yan, Z., Surmeier, D. J., & Foehring, R. C.
Affiliations
Department of Anatomy and Neurobiology, University of Tennessee at Memphis, Memphis, Tennessee 38163
ORCID
Venue
Journal of Neurophysiology
Publisher
American Physiological Society
Published
1999-01-01
Volume
81
Issue
1
Pages
72-84
ISSN
  • 0022-3077 (ISSN-L)
  • 1522-1598
DOI
10.1152/jn.1999.81.1.72
OpenAlex
W2332577778
Cited by
42
References
77
Citations by year
2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
1.49
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Neurophysiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Neurological Disorders and Stroke (NS-33579, NS 34696, R01NS033579)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
muscarine, muscarinic receptors, calcium channel currents, sensorimotor cortex, pyramidal neurons, N-type calcium channels, P-type calcium channels, L-type calcium channels, G proteins, pertussis toxin, atropine, intracellular calcium, voltage dependence, receptor desensitization
MeSH terms
Animals, Calcium Channel Blockers, Cells, Cultured, Dose-Response Relationship, Drug, Electric Stimulation, Electrophysiology, Membrane Potentials, Motor Neurons, Muscarine, Neural Pathways, Receptors, Muscarinic, Calcium Channels, Rats, Sprague-Dawley, Pyramidal Cells, Patch-Clamp Techniques, Muscarinic Agonists, GTP-Binding Proteins, Reverse Transcriptase Polymerase Chain Reaction, Rats

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