Lactobacillus rhamnosus GG Suppresses Meningitic E. coli K1 Penetration across Human Intestinal Epithelial Cells In Vitro and Protects Neonatal Rats against Experimental Hematogenous Meningitis
Open accessAbstract
The purpose of this study was to examine prophylactic efficacy of probiotics in neonatal sepsis and meningitis caused by E. coli K1. The potential inhibitory effect of Lactobacillus rhamnosus GG (LGG) on meningitic E. coli K1 infection was examined by using (i) in vitro inhibition assays with E44 (a CSF isolate from a newborn baby with E. coli meningitis), and (ii) the neonatal rat model of E. coli sepsis and meningitis. The in vitro studies demonstrated that LGG blocked E44 adhesion, invasion, and transcytosis in a dose-dependent manner. A significant reduction in the levels of pathogen colonization, E. coli bacteremia, and meningitis was observed in the LGG-treated neonatal rats, as assessed by viable cultures, compared to the levels in the control group. In conclusion, probiotic LGG strongly suppresses meningitic E. coli pathogens in vitro and in vivo. The results support the use of probiotic strains such as LGG for prophylaxis of neonatal sepsis and meningitis.
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Caco-2 cells resemble mature enterocyte cells in the small intestine of humans and form crypts, forming a homogeneous typical epithelial monolayer.
“These cells resemble mature enterocyte cells in the small intestine of humans and form crypts, forming a homogeneous typical epithelial monolayer [43-45].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [45]
The Discussion (page not provided) describes Caco-2 cells as a model for small-intestinal epithelial differentiation and says they form a polarized monolayer after differentiation. Section 2.2 (page not provided) likewise describes differentiated monolayers with dome formation. Neither passage says the cells form crypts or that the monolayer is homogeneous; dome formation is not described as crypt formation. (LLM, conf=0.86)
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Cites
Group B Streptococcal Disease in the Era of Intrapartum Antibiotic Prophylaxis
Schrag, S. J., Zywicki, S., Farley, M. M., Reingold, A. L., Harrison, L. H., Lefkowitz, L. B., Hadler, J. L., Danila, R., Cieslak, P. R., & Schuchat, A. (2000) · New England Journal of Medicine · doi:10.1056/nejm200001063420103 · Cited by 1,019 · see citing works
Potential Uses of Probiotics in Clinical Practice
Reid, G., Jaß, J., Sebulsky, M. T., & McCormick, J. K. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.4.658-672.2003 · Cited by 918 · see citing works
Rousset, M. (1986) · Biochimie · doi:10.1016/s0300-9084(86)80177-8 · Cited by 478 · see citing works
Antimicrobial-resistant bacteria in the community setting
Furuya, E. Y., & Lowy, F. D. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1325 · Cited by 386 · see citing works
Probiotic agents and infectious diseases: a modern perspective on a traditional therapy
Alvarez-Olmos, M. I., & Oberhelman, R. A. (2001) · Clinical Infectious Diseases · doi:10.1086/320518 · Cited by 359 · see citing works
Isolauri, E. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.6.1142s · Cited by 291 · see citing works
Weiser, J. N., & Gotschlich, E. C. (1991) · Infection and Immunity · doi:10.1128/iai.59.7.2252-2258.1991 · Cited by 256 · see citing works
Devito, C., Broliden, K., Kaul, R., Svensson, L., Johansen, K., Kiama, P., Kimani, J., Lopalco, L., Piconi, S., Bwayo, J. J., et al. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.9.5170 · Cited by 249 · see citing works
Chen, S. H. M., Stins, M. F., Huang, S.-H., Chen, Y. H., Kwon-Chung, K. J., Chang, Y., Kim, K. S., Suzuki, K., & Jong, A. Y. (2003) · Journal of Medical Microbiology · doi:10.1099/jmm.0.05230-0 · Cited by 189 · see citing works
Administration of Oral Probiotic Bacteria to Pregnant Women Causes Temporary Infantile Colonization
Schultz, M., Göttl, C., Young, R. J., Iwen, P. C., & Vanderhoof, J. A. (2004) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/00005176-200403000-00012 · Cited by 166 · see citing works
Traversal of Candida albicans across Human Blood-Brain Barrier In Vitro
Jong, A., Stins, M. F., Huang, S., Chen, S. H. M., & Kim, K. S. (2001) · Infection and Immunity · doi:10.1128/iai.69.7.4536-4544.2001 · Cited by 142 · see citing works
Enhanced intestinal absorption of salmon calcitonin (sCT) from proliposomes containing bile salts
Song, K., Chung, S., & Shim, C. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.05.016 · Cited by 140 · see citing works
Bacterial penetration across the blood-brain barrier during the development of neonatal meningitis
Huang, S., Stins, M. F., & Kim, K. S. (2000) · Microbes and Infection · doi:10.1016/s1286-4579(00)01277-6 · Cited by 136 · see citing works
Huang, S., & Jong, A. (2001) · Cellular Microbiology · doi:10.1046/j.1462-5822.2001.00116.x · Cited by 124 · see citing works
Huang, S., Chen, Y.-H., Fu, Q., Stins, M. F., Wang, Y., Wass, C. A., & Kim, K. S. (1999) · Infection and Immunity · doi:10.1128/iai.67.5.2103-2109.1999 · Cited by 123 · see citing works
Vendt, N., Grünberg, H., Tuure, T., Malminiemi, O., Wuolijoki, E., Tillmann, V., Sepp, E., & Korpela, R. (2006) · Journal of Human Nutrition and Dietetics · doi:10.1111/j.1365-277x.2006.00660.x · Cited by 120 · see citing works
Schrag, S. J., Hadler, J. L., Arnold, K. E., Martell-Cleary, P., Reingold, A. L., & Schuchat, A. (2006) · PEDIATRICS · doi:10.1542/peds.2005-3083 · Cited by 116 · see citing works
Effect of probiotics on enterocyte bacterial translocation in vitro
Mattar, A., Drongowski, R. A., Coran, A. G., & Harmon, C. M. (2001) · Pediatric Surgery International · doi:10.1007/s003830100591 · Cited by 112 · see citing works
Enhancement of gastric mucosal integrity by Lactobacillus rhamnosus GG
Lam, E. K. Y., Tai, E. K. K., Koo, M. W. L., Wong, H. P. S., Wu, W. K. K., Yu, L., So, W. H. L., Woo, P. C. Y., & Cho, C. H. (2007) · Life Sciences · doi:10.1016/j.lfs.2007.03.018 · Cited by 110 · see citing works
Huang, S., Wan, Z., Chen, Y., Jong, A., & Kim, K. S. (2001) · The Journal of Infectious Diseases · doi:10.1086/319290 · Cited by 105 · see citing works
Daley, A. J., & Isaacs, D. (2004) · The Pediatric Infectious Disease Journal · doi:10.1097/01.inf.0000128782.20060.79 · Cited by 94 · see citing works
Sastre, J. B. L., Colomer, B. F., Cotallo, G. D. C., & Aparicio, A. R. (2005) · Acta Paediatrica · doi:10.1111/j.1651-2227.2005.tb01917.x · Cited by 93 · see citing works
Alarcón, A., PEÑA, P., SALAS, S., Naranjo, M. S., & Omeñaca, F. (2004) · The Pediatric Infectious Disease Journal · doi:10.1097/00006454-200404000-00004 · Cited by 85 · see citing works
Pine, L., Kathariou, S., Quinn, F. D., George, V., Wenger, J. D., & Weaver, R. E. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.5.990-996.1991 · Cited by 83 · see citing works
Intrapartum antibiotic prophylaxis increases the incidence of gram-negative neonatal sepsis
Levine, E. M., Ghai, V., Barton, J. J., & STROM, C. M. (1999) · Infectious Diseases in Obstetrics and Gynecology · doi:10.1002/(sici)1098-0997(1999)7:4 · Cited by 80 · see citing works
Brückener, K. E., el Bayâ, A., Galla, H., & Schmidt, M. A. (2003) · Journal of Cell Science · doi:10.1242/jcs.00378 · Cited by 77 · see citing works
Effect of Lactobacillus rhamnosus GG in Persistent Diarrhea in Indian Children
Basu, S., Chatterjee, M., Ganguly, S., & Chandra, P. K. (2007) · Journal of Clinical Gastroenterology · doi:10.1097/01.mcg.0000248009.47526.ea · Cited by 75 · see citing works
Prevention of group B streptococcal disease in the newborn.
Apgar, B. S., Greenberg, M. H. S. A. G. M., & Yen, G. (2005) · PubMed · Cited by 46 · see citing works
Transcytosis of Gastrointestinal Epithelial Cells by Escherichia coli K1
Burns, J. L., Griffith, A., Barry, J. J., Jonas, M., & Chi, E. Y. (2001) · Pediatric Research · doi:10.1203/00006450-200101000-00010 · Cited by 37 · see citing works
Manzoni, P. (2007) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/01.mpg.0000302971.06115.15 · Cited by 28 · see citing works
Prevention of neonatal group B streptococcal disease: Progress, challenges and dilemmas
Daley, A. J., & Garland, S. (2004) · Journal of Paediatrics and Child Health · doi:10.1111/j.1440-1754.2004.00507.x · Cited by 16 · see citing works
Taking probiotics during pregnancy. Are they useful therapy for mothers and newborns?
Reid, G., & Kirjaivanen, P. (2005) · Europe PMC (PubMed Central) · Cited by 15 · see citing works
The evolving role of infectomics in drug discovery
Huang, S., Wang, X., & Jong, A. (2007) · Expert Opinion on Drug Discovery · doi:10.1517/17460441.2.7.961 · Cited by 6 · see citing works
&NA; (2001) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/00005176-200109000-00038 · Cited by 4 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [1]Huang S-H Chen YH Zhang SX Shen YF E. coli invasion of the blood-brain barrier Advances in Medical Molecular Biology 1999 2 Beijing, China Verlag Heidelberg: China Higher Education Press and Springer 114 136
- [33]Pietzak MM Badger J Huang S-H Thomas DW Shimada H Kim KS Inderlied C. Escherichia coli K1 IbeA is required for efficient intestinal epithelial invasion in vitro and in vivo in neonatal rats Journal of Pediatric Gastroenterology and Nutrition 2001 33 p. 400
Cited by
Sorted by citations
Mechanisms of action of probiotics
Plaza-Diaz, J., Ruiz-Ojeda, F. J., Gil-Campos, M., & Gil, A. (2019) · Advances in Nutrition · doi:10.1093/advances/nmy063 · Cited by 1,423 · see citing works
Sources, isolation, characterisation and evaluation of probiotics
Fontana, L., Bermudez-Brito, M., Plaza-Diaz, J., Muñoz-Quezada, S., & Gil, A. (2013) · British Journal of Nutrition · doi:10.1017/s0007114512004011 · Cited by 348 · see citing works
Mechanisms and therapeutic effectiveness of lactobacilli
Cerbo, A. D., Palmieri, B., Aponte, M., Morales‐Medina, J. C., & Iannitti, T. (2015) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2015-202976 · Cited by 264 · see citing works
Mathipa-Mdakane, M. G., & Thantsha, M. S. (2022) · Foods · doi:10.3390/foods11060785 · Cited by 89 · see citing works
He, X., Zeng, Q., Puthiyakunnon, S., Zeng, Z., Yang, W., Qiu, J., Du, L., Boddu, S., Wu, T., Cai, D., et al. (2017) · Scientific Reports · doi:10.1038/srep43305 · Cited by 54 · see citing works
Preservation of viability and antibacterial activity of Lactobacillus spp. in calcium alginate beads
Brachkova, M. I., Duarte, M. A., & Pinto, J. F. (2010) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2010.08.008 · Cited by 47 · see citing works
Kathayat, D., Closs, G., Helmy, Y. A., Deblais, L., Srivastava, V., & Rajashekara, G. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-021-09840-1 · Cited by 39 · see citing works
Chingwaru, W., & Vidmar, J. (2016) · Asian Pacific Journal of Tropical Medicine · doi:10.1016/j.apjtm.2016.12.009 · Cited by 34 · see citing works
Mahboobi, S., Ghasvarian, M., Ghaem, H., Alipour, H., Alipour, S., & Eftekhari, M. H. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.1018357 · Cited by 31 · see citing works
Ling, N., Wang, X., Liu, D., Shen, Y., Zhang, D., Ou, D., Fan, H., Wang, J., Ding, Y., Zhang, J., et al. (2021) · Food and Chemical Toxicology · doi:10.1016/j.fct.2020.111940 · Cited by 30 · see citing works
Review: The market of probiotics.
Cerbo, A. D., & Palmieri, B. (2015) · PubMed · Cited by 30 · see citing works
Yu, J., Zhang, B., Peng, L., Wu, C., Cao, H., Zhong, J. F., Hoffman, J. A., & Huang, S. (2015) · PLoS ONE · doi:10.1371/journal.pone.0121911 · Cited by 31 · see citing works
Route of infection alters virulence of neonatal septicemia Escherichia coli clinical isolates
Cole, B. K., Scott, E. J., Ilikj, M., Bard, D., Akins, D. R., Dyer, D. W., & Chávez‐Bueno, S. (2017) · PLoS ONE · doi:10.1371/journal.pone.0189032 · Cited by 29 · see citing works
Simões, L., Fernandes, N., de Souza, A., dos Santos, L., Magnani, M., Abrunhosa, L., Teixeira, J., Schwan, R. F., & Dias, D. R. (2022) · Fermentation · doi:10.3390/fermentation8060277 · Cited by 23 · see citing works
Peng, L., Luo, W., Tie, Z., Wan, C., Jiang, Y., Chi, F., Zhao, W., Cao, H., & Huang, S. (2012) · Future Microbiology · doi:10.2217/fmb.12.3 · Cited by 23 · see citing works
Effects of heparosan and heparin on the adhesion and biofilm formation of several bacteria in vitro
Chen, X., Ling, P., Duan, R., & Zhang, T. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.02.006 · Cited by 17 · see citing works
Kaur, A., Chopra, K., Kaur, I. P., & Rishi, P. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01568 · Cited by 16 · see citing works
Lin, R., Zhu, B., Zhang, Y., Bai, Y., Zhi, F., Long, B., Li, Y., Wu, Y., Wu, X., & Fan, H. (2017) · Microbial Pathogenesis · doi:10.1016/j.micpath.2017.01.062 · Cited by 13 · see citing works
Antoine, C., Laforêt, F., Goya‐Jorge, E., Gonza, I., Lebrun, S., Douny, C., Duprez, J.-N., Fall, P. A., Taminiau, B., Scippo, M., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241310580 · Cited by 9 · see citing works
Sagi, S., Balakrishna, K., & Parida, M. (2020) · International Immunopharmacology · doi:10.1016/j.intimp.2020.106617 · Cited by 9 · see citing works
The benefits, limitations and opportunities of preclinical models for neonatal drug development
Campion, S. N., Inselman, A. L., Hayes, B. A., Casiraghi, C., Joseph, D., Facchinetti, F., Salomone, F., Schmitt, G., Hui, J. Y., Davis-Bruno, K. L., et al. (2022) · Disease Models & Mechanisms · doi:10.1242/dmm.049065 · Cited by 9 · see citing works
Hidayat, K., Zhang, L., Wei, H., Zhang, W., Qin, L., Ou, Y., & Li, N. (2025) · Food & Function · doi:10.1039/d5fo01780g · Cited by 5 · see citing works
Xuan, H. M., Umar, S., Zhong, C., Yu, W., Ahmed, I., Wheatley, J. L., Sampath, V., & Chávez‐Bueno, S. (2024) · BMC Microbiology · doi:10.1186/s12866-024-03598-6 · Cited by 5 · see citing works
Probiotics in Neonatal Sepsis Associated with Necrotizing Enterocolitis and Meningitis
Huang, S., Cao, H., & Jong, A. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-374938-3.00018-9 · Cited by 4 · see citing works
Tezera, L. B., Keith, P., Adelina, R., Vyvyan, S., & Davenport, V. (2014) · Advances in Microbiology · doi:10.4236/aim.2014.42013 · Cited by 3 · see citing works
Epidemiology of Early and Late Onset Neonatal Sepsis
Park, S. E. (2012) · Journal of the Korean Society of Neonatology · doi:10.5385/jksn.2012.19.3.115 · Cited by 2 · see citing works
Colla, E. C. X., Meneguzzi, D., Mota, T. F. M., Dutra, J. D. C. F., Carpes, S. T., Appelt, P., Trentin, A. B., Marchioro, M. L. K., Dekker, R. F. H., & da Cunha, M. A. A. (2025) · Fermentation · doi:10.3390/fermentation11020063 · Cited by 1 · see citing works
Shekar, A., Ramlal, S., Kingston, J. J., & Sripathy, M. H. (2018) · International Immunopharmacology · doi:10.1016/j.intimp.2018.08.001 · Cited by 1 · see citing works
Evaluation of the viability of Lactobacillus spp. in different dosage forms
Brachkova, M. I. (2010) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)
Role of microbiota-gut-brain axis in brain infections and possible interventions
Sharma, P., Sharma, B., Vishwakarma, K., Setty, A. R. R., Sandeep, P., Dhiman, N., & Arora, S. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-21680-0.00012-2
- Authors
- Huang, S.-H., He, L., Zhou, Y., Wu, C.-H., & Jong, A.
- Affiliations
- University of Southern California (US)
- Huazhong University of Science and Technology (CN)
- Department of Pediatrics, The Saban Research Institute of Childrens Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA
- Corresponding author
- Huang, Sheng-He
- Venue
- International Journal of Microbiology
- Publisher
- Hindawi Limited
- Published
- 2009
- Volume
- 2009
- Pages
- 1-7
- ISSN
- 1687-918X (ISSN-L)
- 1687-9198
- PMC
- PMC2775688
- OpenAlex
- W2002568542
- Cited by
- 30
- References
- 34
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 2 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- International Journal of Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- United States Public Health Service (R21AT003207, R01-AI40635, R01 NS047599)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus rhamnosus GG, probiotics, Escherichia coli K1, meningitis, neonatal sepsis, intestinal epithelial cells, bacterial adhesion, bacterial invasion, transcytosis, bacteremia, neonatal rat model, prophylaxis
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