Biochemical Modulation of Lipid Pathway in Microalgae Dunaliella sp. for Biodiesel Production
Open accessAbstract
Exploitation of renewable sources of energy such as algal biodiesel could turn energy supplies problem around. Studies on a locally isolated strain of Dunaliella sp. showed that the mean lipid content in cultures enriched by 200 mg L−1 myoinositol was raised by around 33% (1.5 times higher than the control). Similarly, higher lipid productivity values were achieved in cultures treated by 100 and 200 mg L−1 myoinositol. Fluorometry analyses (microplate fluorescence and flow cytometry) revealed increased oil accumulation in the Nile red-stained algal samples. Moreover, it was predicted that biodiesel produced from myoinositol-treated cells possessed improved oxidative stability, cetane number, and cloud point values. From the genomic point of view, real-time analyses revealed that myoinositol negatively influenced transcript abundance of AccD gene (one of the key genes involved in lipid production pathway) due to feedback inhibition and that its positive effect must have been exerted through other genes. The findings of the current research are not to interprete that myoinositol supplementation could answer all the challenges faced in microalgal biodiesel production but instead to show that “there is a there there” for biochemical modulation strategies, which we achieved, increased algal oil quantity and enhanced resultant biodiesel quality.
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The addition of myo-inositol to Dunaliella sp. cultures increases cellular lipid content by 33%.
“For example, studies have shown that the addition of myo-inositol to Dunaliella sp. cultures increases cellular lipid content by 33% [104].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [104]
Section 3.2 (page unavailable) reports that myo-inositol increased lipid content in Dunaliella sp., but the 33% figure is the resulting mean lipid content, not the increase. The paper states that this represents a 50% increase over the control, whose lipid content was 22%. (LLM, conf=0.92)
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- Authors
- Talebi, A. F., Tohidfar, M., Mousavi Derazmahalleh, S. M., Sulaiman, A., Baharuddin, A. S., & Tabatabaei, M.
- Affiliations
- Semnan University (IR)
- Biofuel Research Team (IR)
- Genomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran
- Universiti Teknologi MARA System (MY)
- Universiti Putra Malaysia (MY)
- Corresponding author
- Baharuddin, Azhari Samsu
- Tabatabaei, Meisam
- Venue
- BioMed Research International
- Publisher
- Hindawi Limited
- Published
- 2015
- Volume
- 2015
- Pages
- 1-12
- ISSN
- 1110-7243 (ISSN-L)
- 2314-6133
- 2314-6141
- PMC
- PMC4469783
- OpenAlex
- W1586718308
- Cited by
- 32
- References
- 59
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2021: 8 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 4
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- BioMed Research International (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Agricultural Biotechnology Research Institute of Iran
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Dunaliella, microalgae, algal biodiesel, lipid accumulation, lipid productivity, Nile Red staining, biodiesel quality, oxidative stability, cetane number, cloud point, product inhibition, mRNA levels, biochemical modulation
- MeSH terms
- Biotechnology, Fatty Acids, Humans, Lipids, Signal Transduction, Biomass, Lipid Metabolism, Biofuels, Microalgae
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