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Cited by 65Gut microbiota and healthAsthma and respiratory diseasesDermatology and Skin Diseases

Alterations of the murine gut microbiome with age and allergic airway disease

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Vital, Marius, Harkema, Jack R., Rizzo, Mike, Tiedje, James, Brandenberger, Christina·2015·Journal of Immunology Research
DOI 10.1155/2015/892568PMC PMC4451525
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Abstract

The gut microbiota plays an important role in the development of asthma. With advanced age the microbiome and the immune system are changing and, currently, little is known about how these two factors contribute to the development of allergic asthma in the elderly. In this study we investigated the associations between the intestinal microbiome and allergic airway disease in young and old mice that were sensitized and challenged with house dust mite (HDM). After challenge, the animals were sacrificed, blood serum was collected for cytokine analysis, and the lungs were processed for histopathology. Fecal pellets were excised from the colon and subjected to 16S rRNA analysis. The microbial community structure changed with age and allergy development, where alterations in fecal communities from young to old mice resembled those after HDM challenge. Allergic mice had induced serum levels of IL-17A and old mice developed a greater allergic airway response compared to young mice. This study demonstrates that the intestinal bacterial community structure differs with age, possibly contributing to the exaggerated pulmonary inflammatory response in old mice. Furthermore, our results show that the composition of the gut microbiota changes with pulmonary allergy, indicating bidirectional gut-lung communications.

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  • The species, strain, substrain, sex, weight, and age of animals are critical factors that can influence most experimental results.

    “The species, strain, substrain, sex, weight, and age of animals are critical factors that can influence most experimental results [103–107].”

    From Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0 (2020) · cites this paper as [105]

    Results §3.3 (page not available) identifies age as a significant factor in murine gut microbiome differences, and Results §3.1 reports greater allergic airway inflammation in old mice. The paper does not establish that species, strain, substrain, sex, or weight influence results, or that these factors affect most experimental results. (LLM, conf=0.85)

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References 28 · Cited by 65

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Authors
Vital, M., Harkema, J. R., Rizzo, M., Tiedje, J., & Brandenberger, C.
Affiliations
  • Helmholtz Centre for Infection Research (DE)
  • Michigan State University (US)
  • Medizinische Hochschule Hannover (DE)
Corresponding author
Brandenberger, Christina
ORCID
Venue
Journal of Immunology Research
Publisher
Wiley
Published
2015
Volume
2015
Pages
1-8
ISSN
  • 2314-7156 (ISSN-L)
  • 2314-8861
DOI
10.1155/2015/892568
PMC
PMC4451525
OpenAlex
W1947199013
Cited by
65
References
28
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 1 · 2022: 6 · 2021: 5 · 2020: 11 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 13 · 2015: 1
FWCI
3.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Immunology Research (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HZI (Helmholtz Centre for Infection Research) (submittedVersion, cc-by-nc-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Michigan University Research Corridor (R83479701, UH3 DK083993)
  • National Institutes of Health (R83479701, UH3 DK083993)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords
gut microbiota, aging, allergic airway disease, asthma, house dust mites, IL-17A, gut-lung axis, lung inflammation, microbial community structure, 16S rRNA
MeSH terms
Gastrointestinal Microbiome, Age Factors, Animals, Asthma, Disease Models, Animal, Hypersensitivity, Intestines, Lung, Male, Mice, Inbred BALB C, Pneumonia, RNA, Ribosomal, 16S, Interleukin-17, Pyroglyphidae, Mice

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