Cited by 10,100Gut microbiota and healthOral microbiology and periodontitis researchGastrointestinal motility and disorders

Diet rapidly and reproducibly alters the human gut microbiome

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David, Lawrence A., Maurice, Corinne F., Carmody, Rachel N., Gootenberg, David B., Button, Julie E., Wolfe, Benjamin E., Ling, Alisha V., Devlin, A. Sloan +5 more·2014·Nature

Abstract

Long-term dietary intake influences the structure and activity of the trillions of microorganisms residing in the human gut, but it remains unclear how rapidly and reproducibly the human gut microbiome responds to short-term macronutrient change. Here we show that the short-term consumption of diets composed entirely of animal or plant products alters microbial community structure and overwhelms inter-individual differences in microbial gene expression. The animal-based diet increased the abundance of bile-tolerant microorganisms (Alistipes, Bilophila and Bacteroides) and decreased the levels of Firmicutes that metabolize dietary plant polysaccharides (Roseburia, Eubacterium rectale and Ruminococcus bromii). Microbial activity mirrored differences between herbivorous and carnivorous mammals, reflecting trade-offs between carbohydrate and protein fermentation. Foodborne microbes from both diets transiently colonized the gut, including bacteria, fungi and even viruses. Finally, increases in the abundance and activity of Bilophila wadsworthia on the animal-based diet support a link between dietary fat, bile acids and the outgrowth of microorganisms capable of triggering inflammatory bowel disease. In concert, these results demonstrate that the gut microbiome can rapidly respond to altered diet, potentially facilitating the diversity of human dietary lifestyles.

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Crossref · 10.1038/nature12820

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55 of 55 references verified0 citation errors · 0 not checkable

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  • Both red meat and animal protein decrease levels of Firmicutes, while high dietary fiber increase them.

    “Both red meat and animal protein decrease levels of Firmicutes, while high dietary fiber increase them [133].”

    From The Pathophysiology of Gestational Diabetes Mellitus (2018) · cites this paper as [133]

    The paper’s Abstract reports that an animal-based diet reduced certain Firmicutes that metabolize plant polysaccharides. It does not establish that red meat or animal protein independently caused the decrease, or that Firmicutes as a whole decreased. The passages report higher fiber intake on the plant-based diet, but not an increase in Firmicutes attributable to fiber; no page number is provided in our copy. (LLM, conf=0.86)

    Experimental — accuracy not yet measured
  • Individuals on a Western diet share a similar gut microbiota profile to that observed in obese individuals.

    “Individuals on a Western diet share a similar gut microbiota profile to that observed in obese individuals (David et al., 2014).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as David et al., 2014

    The passage labeled Abstract (page unavailable) says that a Western diet may alter the gut microbiome and that diet-induced changes are suspected of contributing to obesity. It does not say that people eating a Western diet have a gut microbiota profile similar to that of obese individuals; the reported diet intervention makes no such comparison. (LLM, conf=0.82)

    Experimental — accuracy not yet measured
  • Individuals on either an animal- or plant-based diet, when switched to the other diet, experienced substantial changes in gut microbiota composition within 24 hours.

    “For instance, individuals on either an animal-or plant-based diet, when switched to the other diet, experienced substantial changes in the gut microbiota composition, within 24 hours (David et al., 2014).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as David et al., 2014

    The study-design passage and Methods (Diet design) describe separate five-day diet arms, with subjects eating normally outside them—not a direct switch from one experimental diet to the other. The microbial-community passage reports a significant compositional change unique to the animal-based diet, occurring one day after that diet reached the distal gut. It does not establish substantial changes within 24 hours for switches in both directions. (LLM, conf=0.90)

    Experimental — accuracy not yet measured
  • Intake of an animal-based diet shows significant increases in bile-tolerant anaerobes including Alistipes, Bilophila, and Bacteroides.

    “Intake of an animal-based diet showed significant increases in bile-tolerant anaerobes including Alistipes, Bilophila and Bacteroides (Cotillard et al., 2013;David et al., 2014).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as David et al., 2014

  • The secondary bile acid DCA promotes DNA damage and hepatic carcinoma.

    “The secondary BA DCA is known to promote DNA damage and hepatic carcinoma and accumulates significantly on an animal product-based diet in humans [246].”

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Cites

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2 of the 57 references couldn’t be matched to a known paper.

Research works printed but not linked: 5

  1. [22]Bourdichon F Berger B Casaregola S Safety Demonstration of Microbial Food Cultures (MFC) in Fermented Food Products Bulletin of the International Dairy Federation 2012 455
  2. [5]Caporaso JG QIIME allows analysis of high-throughput community sequencing data Nat Methods 2010 7 335 336 PMC3156573 20383131
  3. [12]Maurice CF Haiser HJ Turnbaugh PJ Xenobiotics shape the physiology and gene expression of the active human gut microbiome Cell 2013 152 39 50 23332745 PMC3552296
  4. [13]Turnbaugh PJ The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice Sci Transl Med 2009 1 6ra14 PMC2894525 20368178
  5. [23]Devkota S Dietary-fat-induced taurocholic acid promotes pathobiont expansion and colitis in Il10−/− mice Nature 2012 487 104 108 22722865 PMC3393783

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Authors
David, L. A., Maurice, C. F., Carmody, R. N., Gootenberg, D. B., Button, J. E., Wolfe, B. E., Ling, A. V., Devlin, A. S., Varma, Y., Fischbach, M. A., Biddinger, S. B., Dutton, R. J., & Turnbaugh, P. J.
Affiliations
  • Harvard University (US)
  • Boston Children's Hospital (US)
  • QB3 (US)
Corresponding author
Turnbaugh, Peter J.
Venue
Nature
Published
2013-12-11
Volume
505
Issue
7484
Pages
559-563
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2166562121
Cited by
10,100
References
57
Citations by year
2026: 688 · 2025: 802 · 2024: 924 · 2023: 933 · 2022: 1054 · 2021: 1212 · 2020: 1056 · 2019: 871 · 2018: 737 · 2017: 634 · 2016: 505 · 2015: 420 · 2014: 245 · 2013: 5 · 2012: 1
FWCI
175.57
Type
journal article
Language
en
Open access
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institute of General Medical Sciences (P50GM068763)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK0046200, P30DK046200)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
gut microbiota, dietary intervention, plant-based diet, animal-based diet, macronutrient composition, Bilophila wadsworthia, inflammatory bowel disease, microbial gene expression, dietary fat, bile acids, Firmicutes, Roseburia, Eubacterium rectale, Ruminococcus bromii
MeSH terms
Adult, Bacteria, Bacteroides, Bile Acids and Salts, Diet, Dietary Fats, Feces, Female, Fermentation, Food Microbiology, Humans, Male, Time Factors, Diet, Vegetarian, Inflammatory Bowel Diseases, Gene Expression Regulation, Bacterial, Gastrointestinal Tract, Bilophila, Metagenome, Young Adult, Herbivory, Carnivory, Microbiota

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