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Cited by 28Bone Metabolism and DiseasesAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic Diseases

N-Acetylcysteine (NAC) Ameliorates Lipid-Related Metabolic Dysfunction in Bone Marrow Stromal Cells-Derived Adipocytes

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Raffaele, Marco, Barbagallo, Ignazio, Licari, Maria, Carota, Giuseppe, Sferrazzo, Giuseppe, Spampinato, Mariarita, Sorrenti, Valeria, Vanella, Luca·2018·Evidence-Based Complementary and Alternative Medicine
DOI 10.1155/2018/5310961PMC PMC6207898
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Abstract

Recent experimental data suggest that fatty acids and lipotoxicity could play a role in the initiation and evolution of metabolic bone diseases such as osteoporosis. A functional bone marrow adipose tissue (BMAT) may provide support to surrounding cells and tissues or may serve as a lipid reservoir that protects skeletal osteoblasts from lipotoxicity. The present study examined the effect of N‐acetylcysteine (NAC), a powerful antioxidant and precursor of glutathione, commonly used to treat chronic obstructive pulmonary disease, on triglycerides accumulation in bone marrow stromal cells‐derived adipocytes. Quantification of Oil Red O stained cells showed that lipid droplets decreased following NAC treatment. Additionally, exposure of bone marrow stromal cells (HS‐5) to NAC increased adiponectin, PPAR γ, HO‐1, and SIRT‐1 and increased beta‐oxidation markers such as PPAR α and PPAR δ mRNA levels. As there is now substantial interest in alternative medicine, the observed therapeutic value of NAC should be taken into consideration in diabetic patients.

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  • Relative gene expression levels were normalized with the housekeeping gene GAPDH using the comparative 2−ΔΔCt evaluation method.

    “The relative gene expression levels of each PCR product were obtained according to the threshold cycle (Ct) value and furtherly normalized with the housekeeping gene GAPDH (glyceraldeyde-3-phosphate dehydrogenase) using the comparative 2−ΔΔCt evaluation method [24].”

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Authors
Raffaele, M., Barbagallo, I., Licari, M., Carota, G., Sferrazzo, G., Spampinato, M., Sorrenti, V., & Vanella, L.
Affiliations
University of Catania (IT)
Corresponding author
Vanella, Luca
ORCID
Venue
Evidence-Based Complementary and Alternative Medicine
Publisher
Wiley
Published
2018-01
Volume
2018
Issue
1
Pages
5310961-5310961
ISSN
  • 1741-427X (ISSN-L)
  • 1741-4288
DOI
10.1155/2018/5310961
PMC
PMC6207898
OpenAlex
W2896420505
Cited by
28
References
1
Citations by year
2026: 2 · 2025: 6 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 8
FWCI
1.16
Type
journal article
Language
en
Open access
Licence
Locations
  • Evidence-based Complementary and Alternative Medicine (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
N-acetylcysteine, bone marrow adipose tissue, mesenchymal stem cells, adipocytes, lipotoxicity, triglyceride accumulation, lipid droplets, osteoporosis, metabolic bone disease, adiponectin, PPARγ, PPARα, PPARδ, SIRT1, heme oxygenase-1, fatty acid beta-oxidation, antioxidant therapy, diabetes mellitus

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