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Cited by 19Aortic aneurysm repair treatmentsMicroRNA in disease regulationConnective tissue disorders research

The Expression Profile of MicroRNAs in Small and Large Abdominal Aortic Aneurysms

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Černá, Václava, Ostašov, Pavel, Pitule, Pavel, Moláček, Jiří, Třeška, Vladislav, Pešta, Martin·2019·Cardiology Research and Practice
DOI 10.1155/2019/8645840PMC PMC6914980
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Abstract

Background. Abdominal aortic aneurysms (AAA) are relatively frequent in elderly population, and their ruptures are related with high mortality rate. There are no actually used laboratory markers predicting the AAA development, course, and rupture. MicroRNAs are small noncoding molecules involved in posttranscriptional gene expression regulation, influencing processes on cell and tissue levels, and are actually in focus due to their potential to become diagnostic or prognostic markers in various diseases. Methods. Tissue samples of AAA patients and healthy controls were collected, from which miRNA was isolated. Microarray including the complete panel of 2549 miRNAs was used to find expression miRNA profiles that were analysed in three subgroups: small (N = 10) and large (N = 6) aneurysms and healthy controls (N = 5). Fold changes between expression in aneurysms and normal tissue were calculated including corresponding p values, adjusted to multiple comparisons. Results. Six miRNAs were found to be significantly dysregulated in small aneurysms (miR-7158-5p, miR-658, miR-517-5p, miR-122-5p, miR-326, and miR-3180) and 162 in large aneurysms, in comparison with the healthy control. Ten miRNAs in large aneurysms with more than two-fold significant change in expression were identified: miR-23a-3p, miR-24-3p, miR-27a-3p, miR-27b-3p, miR-30d-5p, miR-193a-3p, miR-203a-3p, miR-365a-3p, miR-4291, and miR-3663-3p and all, but the last one was downregulated in aneurysmal walls. Conclusion. We confirmed some previously identified miRNAs (miR-23/27/24 family, miR-193a, and miR-30) as associated with AAA pathogenesis. We have found other, yet in AAA unidentified miRNAs (miR-203a, miR-3663, miR-365a, and miR-4291) for further analyses, to investigate more closely their possible role in pathogenesis of aneurysms. If their role in AAA development is proved significant in future, they can become potential markers or treatment targets.

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  • Expression levels of miR-23a-3p are increased in abdominal aortic aneurysm.

    “the expression levels of miR-23a-3p are also increased in abdominal aortic aneurysm (AAA)”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [97]

    Section 3.3 (Results) identifies miR-23a-3p among the miRNAs downregulated in large aneurysmal walls compared with healthy controls. The Abstract (Results) reports the same direction of change, contrary to the claimed increase. (LLM, conf=0.90)

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  • The observation of increased miR-23a-3p in abdominal aortic aneurysm is limited to clinical samples and lacks further investigation of its regulation mechanism.

    “this observation is limited to clinical samples and lacks further investigation of its regulation mechanism [97].”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [97]

    Section 3.3 (page unavailable) reports that miR-23a-3p was downregulated, not increased, in large abdominal aortic aneurysm tissue. Although this study analyzed clinical tissue samples, its reported direction of expression contradicts the observation described in the claim. (LLM, conf=0.91)

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Cited by

Sorted by citations

Authors
Černá, V., Ostašov, P., Pitule, P., Moláček, J., Třeška, V., & Pešta, M.
Affiliations
Charles University (CZ)
Corresponding author
Pešta, Martin
ORCID
Venue
Cardiology Research and Practice
Publisher
Wiley
Published
2019-11-29
Volume
2019
Pages
1-8
ISSN
  • 2090-0597 (ISSN-L)
  • 2090-8016
DOI
10.1155/2019/8645840
PMC
PMC6914980
OpenAlex
W2991124544
Cited by
19
References
3
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 3
FWCI
2.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Cardiology Research and Practice (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Univerzita Karlova v Praze
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Aortic aneurysm repair treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Connective tissue disorders research
Keywords
abdominal aortic aneurysm, miRNA expression profiling, miR-193a, miR-30, miR-203a, miR-122-5p, aneurysm rupture, diagnostic biomarkers, post-transcriptional regulation

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