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Cited by 14Natural Antidiabetic Agents StudiesBee Products Chemical AnalysisMedicinal Plant Research

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The Synergistic Beneficial Effect of Thyme Honey and Olive Oil against Diabetes and Its Complications Induced by Alloxan in Wistar Rats

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Lafraxo, Hajar, Bakour, Meryem, Laaroussi, Hassan, El Ghouizi, Asmae, Ousaaid, Driss, Aboulghazi, Abderrazak, Lyoussi, Badiaa·2021·Evidence-Based Complementary and Alternative Medicine
DOI 10.1155/2021/9949056PMC PMC8478563
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Abstract

Diabetes is a metabolic disorder characterized by a chronic increase in blood glucose. Owing to the limitations observed with antidiabetics in modern medicine, medicinal plants and bee products are known as good matrices for the search for new antidiabetic molecules. The present study focused on the evaluation of the hypoglycemic and the protective properties of two natural products widely used in complementary and alternative medicine (thyme honey and olive oil). To achieve this, the study was carried out on Wistar rats rendered diabetic by the injection of a single dose of alloxan monohydrate (65 mg/kg body weight (BW)). First, the physicochemical characterization and the phytochemical analysis of thyme honey and olive oil were carried out, and then in vivo study was conducted on 42 Wistar rats divided into seven groups: three groups were normal, one group was untreated diabetic, and three groups were diabetic rats treated with thyme honey (2 g/kg BW) or olive oil (10 mL/kg BW) or their combination ((1 g/kg BW of thyme honey) and (5 mL/kg BW of olive oil)). During the experiment, the glycemia was measured regularly every 10 days. After 30 days of treatment, the rats were sacrificed. The serum and urine were analyzed to determine hepatic enzymes levels (AST, ALT, ALP, and LDH), lipidic profile (total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein), and kidney parameters (urea, uric acid, creatinine, total protein, sodium, potassium, and chloride). The liver, pancreas, and kidneys were analyzed to evaluate their histological changes and to determine their enzymatic antioxidant content (catalase, GSH, and GPx) and the levels of MDA. The results obtained showed that thyme honey or olive oil, and especially their combination, improved significantly the blood glucose levels and they protect against metabolic changes and the complications induced by diabetes.

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  • FsV contains functional substances that exert their effects in synergy.

    “Interestingly, it contains a wide range of functional substances that exert their effects in synergy [3,20,21].”

    From Fruits Vinegar: Quality Characteristics, Phytochemistry, and Functionality (2021) · cites this paper as [20]

    The Introduction describes numerous compounds in honey and olive oil, and the Conclusion reports that their combination was especially beneficial in diabetic rats. But the paper does not address FsV or establish that its constituent substances act synergistically; the Conclusion says the responsible molecules and mechanism remain unknown. (LLM, conf=0.78)

    Experimental — accuracy not yet measured

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Metadata

Authors
Lafraxo, H., Bakour, M., Laaroussi, H., El Ghouizi, A., Ousaaid, D., Aboulghazi, A., & Lyoussi, B.
Affiliations
  • Sidi Mohamed Ben Abdellah University (MA)
  • Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco
ORCID
Venue
Evidence-Based Complementary and Alternative Medicine
Publisher
Wiley
Published
2021-09-20
Volume
2021
Pages
1-20
ISSN
  • 1741-427X (ISSN-L)
  • 1741-4288
DOI
10.1155/2021/9949056
PMC
PMC8478563
OpenAlex
W3201195070
Cited by
14
References
78
Citations by year
2026: 1 · 2025: 2 · 2023: 4 · 2022: 6 · 2021: 1
FWCI
2.05
Type
journal article
Language
en
Open access
Licence
Locations
  • Evidence-based Complementary and Alternative Medicine (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
University Sidi Mohamed Ben Abdallah (USMBA/PPSE/L08 FSDM 2018)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
thyme honey, olive oil, diabetes mellitus, alloxan-induced diabetes, hypoglycemic effect, diabetes complications, antioxidant enzymes, lipid profile, liver enzymes, renal function, oxidative stress, synergistic effect, bee products, medicinal plants

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