The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non–Small Cell Lung Cancer Using Next-Generation Sequencing
Abstract
Purpose: To identify tumor-derived exosomal biomarkers that are able to discriminate between adenocarcinoma and squamous cell carcinoma (SCC) as a noninvasive method in the early diagnosis of non–small cell lung cancer (NSCLC). Experimental Design: Tumor-derived exosomes from the plasma of early-stage NSCLC patients were isolated. Exosomal miRNA profiling of 46 stage I NSCLC patients and 42 healthy individuals was performed using miRNA-seq to identify and validate adenocarcinoma- and SCC-specific miRNAs. The diagnostic accuracy of select miRNAs was tested further with an additional 60 individuals. Results: There were 11 and 6 miRNAs expressed at remarkably higher levels, 13 and 8 miRNAs expressed at lower levels in adenocarcinoma and SCC patients, respectively, compared with healthy volunteers. Distinct adenocarcinoma- and SCC-specific exosomal miRNAs were validated. The reliability of miRNA-seq data was verified with several demonstrated diagnostic potential miRNAs for NSCLC and other carcinomas, as reported in previous studies, such as let-7, miR-21, miR-24, and miR-486. The results indicated that miR-181-5p, miR-30a-3p, miR-30e-3p, and miR-361-5p were adenocarcinoma-specific, and miR-10b-5p, miR-15b-5p, and miR-320b were SCC-specific. The diagnostic accuracy of three combination miRNA panels was evaluated using an AUC value of 0.899, 0.936, and 0.911 for detecting NSCLC, adenocarcinoma, and SCC, respectively. Conclusions: Tumor-derived exosomal miRNAs, adenocarcinoma-specific miR-181-5p, miR-30a-3p, miR-30e-3p and miR-361-5p, and SCC-specific miR-10b-5p, miR-15b-5p, and miR-320b were observed by next-generation sequencing, and their diagnostic accuracy were verified. These miRNAs may be promising and effective candidates in the development of highly sensitive, noninvasive biomarkers for early NSCLC diagnosis. Clin Cancer Res; 23(17); 5311–9. ©2017 AACR.
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Cancer statistics in China, 2015
Chen, W., Zheng, R., Baade, P. D., Zhang, S., Zeng, H., Bray, F., Jemal, A., Yu, X. Q., & He, J. (2016) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.21338 · Cited by 17,038
Reduced Lung-Cancer Mortality with Low-Dose Computed Tomographic Screening
The National Lung Screening Trial Research Team (2011) · New England Journal of Medicine · doi:10.1056/nejmoa1102873 · Cited by 11,064
Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., & Thun, M. J. (2009) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.20006 · Cited by 9,709
Circulating Exosomal microRNAs as Biomarkers of Colon Cancer
Ogata-Kawata, H., Izumiya, M., Kurioka, D., Honma, Y., Yamada, Y., Furuta, K., Gunji, T., Ohta, H., Okamoto, H., Sonoda, H., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0092921 · Cited by 809
Cited by
Li, M.-Y., Liu, L.-Z., & Dong, M. (2021) · Molecular Cancer · doi:10.1186/s12943-021-01312-y · Cited by 215
Self-citationGeekiyanage, H., Rayatpisheh, S., Wohlschlegel, J. A., Brown, R., & Ambros, V. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2008323117 · Cited by 148
Han, F., Huang, D., Huang, X., Wang, W., Yang, S., & Chen, S. (2020) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.15402 · Cited by 42
Long, L., Zhang, X., Bai, J., Li, Y., Wang, X., & Zhou, Y. (2019) · Cancer Management and Research · doi:10.2147/cmar.s198966 · Cited by 21
Lung cancer-derived exosomal miR-132-3p contributed to interstitial lung disease development
Fang, S., Wang, T., Weng, L., Han, X., Zheng, R., & Zhang, H. (2023) · World Journal of Surgical Oncology · doi:10.1186/s12957-023-03095-6 · Cited by 13
Glioblastoma exosomes reprogramming the tumor microenvironment and evading therapeutic challenges
Tatode, A., Premchandani, T., Chaudhary, A. A., Ali, M. A. M., Qutub, M., Taksande, J., Khan, R., Hussain, U. M., & Umekar, M. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-11526-2 · Cited by 3
Metadata
- Authors
- Jin, X., Chen, Y., Chen, H., Fei, S., Chen, D., Cai, X., Liu, L., Lin, B., Su, H., Zhao, L., Su, M., Pan, H., Shen, L., Xie, D., & Xie, C.
- Venue
- Clinical Cancer Research
- Published
- 2017-08-31
- Volume
- 23
- Issue
- 17
- Pages
- 5311-5319
- ISSN
- 1078-0432 (ISSN-L)
- 1557-3265
- OpenAlex
- W2626420049
- Cited by
- 558
- References
- 4
- FWCI
- 18.73
- Type
- journal article
- Language
- en
- Open access
- closed
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