Immunomodulatory Effects of Lactobacillus and Bifidobacterium on Both Murine and Human Mitogen-Activated T Cells
Abstract
Background: Beneficial effects of probiotics have been reported for patients with allergic diseases and intestinal disorders. There is increasing interest in studying the role of different strains or combined probiotic administration on immunoregulation. In this study, we investigated whether probiotics modulate the immune response through regulating T cell proliferation and differentiation. Methods: We examined the effect of probiotic I (a combination of Lactobacillus acidophilus and Bifidobacterium bifidus) and probiotic II (a combination of L. acidophilus and B. infantis) on cell survival and proliferation, the progression of the cell cycle, and the production of Th1/Th2 cytokines by mitogen-stimulated murine spleen cells and human peripheral blood mononuclear cells (PBMCs). Results: Our experimental results showed that high concentrations (≧1 × 10 6 CFU/ml) of probiotic I or II inhibited mitogen-induced cell proliferation and arrested the cell cycle at the G0/G1 stage in both mitogen-stimulated spleen cells and PBMCs. In the results of low concentrations (<1 × 10 6 CFU/ml), probiotic I or II enhanced the production of IFN-γ but inhibited the production of IL-4. Our results indicated that high concentrations of probiotic I or II treatment could attenuate mitogen-induced overactive immune responses. On the other hand, low concentrations of probiotic I or II treatment could promote a shift in the Th1/Th2 balance toward Th1-skewed immunity. Conclusion: Dose selection is an important issue for probiotic studies. Our results indicated that probiotics have beneficial effects on regulating T cell-mediated immune responses by attenuating mitogen-induced overactive immune responses and promoting Th1 immune responses.
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The regulation of T-cell-mediated immune responses has also been observed in potential probiotic LAB.
“The regulation of T-cell-mediated immune responses has also been observed in the potential probiotic LAB [37,65].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [65]
Humans likely respond to psychosocial stress with gut microbiome responses comparable to, if distinct from, those of laboratory animals.
“it seems likely that humans respond to psychosocial stress with responses that are comparable to, if distinct from, the reactions of laboratory animals.”
From The Gut Microbiome and the Brain (2014) · cites this paper as [124]
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- Authors
- Li, C.-Y., Lin, H.-C., Lai, C.-H., Lu, J. J.-Y., Wu, S.-F., & Fang, S.-H.
- Affiliations
- National Health Research Institutes (TW)
- China Medical University Hospital (TW)
- China Medical University (TW)
- National Taiwan University of Sport (TW)
- Venue
- International Archives of Allergy and Immunology
- Publisher
- S. Karger AG
- Published
- 2011
- Volume
- 156
- Issue
- 2
- Pages
- 128-136
- ISSN
- 1018-2438 (ISSN-L)
- 1423-0097
- OpenAlex
- W2076307872
- Cited by
- 37
- References
- 45
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 5 · 2012: 2
- FWCI
- 5.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Archives of Allergy and Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- China Medical University Hospital
- China Medical University, Taiwan
- Division of Materials Research
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- probiotics, Lactobacillus acidophilus, Bifidobacterium bifidum, Bifidobacterium infantis, T cell proliferation, cell cycle arrest, G0/G1 arrest, Th1/Th2 balance, interferon-gamma, IL-4, dose-dependent effects, immunomodulation, T cell differentiation
- MeSH terms
- Animals, Bifidobacterium, Cell Cycle, Cell Survival, Flow Cytometry, Humans, Immunologic Factors, Interferon-gamma, Lactobacillus, Leukocytes, Mononuclear, Lymphocyte Activation, Mice, Inbred BALB C, T-Lymphocytes, Interleukin-4, Probiotics, Mice
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